Effects of lanthanum and gadolinium on proliferation and differentiation of primary osteoblasts

作     者：Zhang Dawei , Zhang Jinchao , Chen Yao , Yang Mengsu and Yao Xinsheng (Department of Natural Products Chemistry, Shenyang Pharmaceutical University, Shenyang 110016, China Shenzhen Research Institute of City University of Hong Kong, Shenzhen 518057, China Department of Biology and Chemistry, City University of Hong Kong, China Department of Chemistry, College of Chemistry & Environmental Science, Hebei University, Baoding 071002, China Key Laboratory for Research and Development of Traditional Chinese Medicine and Natural Medicine at Shenzhen, Shenzhen 518057, China) 

作者机构：Department of Natural Products Chemistry Shenyang Pharmaceutical University Shenyang 110016 China Shenzhen Research Institute of City University of Hong Kong Shenzhen 518057 China Department of Biology and Chemistry City University of Hong Kong China Department of Chemistry College of Chemistry & Environmental Science Hebei University Baoding 071002 China Key Laboratory for Research and Development of Traditional Chinese Medicine and Natural Medicine at Shenzhen Shenzhen 518057 China 

出 版 物：《Progress in Natural Science:Materials International》 (自然科学进展·国际材料(英文))

年 卷 期：2007年第17卷第5期

页      面：618-623页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 070301[理学-无机化学] 0702[理学-物理学] 

基　　金：Supported by National Natural Science Foundation of China (Grant No. 30418007) 

主　　题：lanthanum gadolinium osteoblast-like cells proliferation differentiation transdifferentiation. 

摘      要：The effects of La3+ and Gd3+ on the proliferation, differentiation and adipogenic transdifferentiation of rat calvarial osteoblast-like cells (ROB cells) were evaluated by MTT method, measuring the activity of alkaline phosphatase (ALP) and Oil red O measurement. Both of La3+ and Gd3+ inhibited the proliferation of ROB cells at all concentrations (1×10-5, 1×10-6, 1×10-7, 1×10-8, 1×10-9 mol·L-1). La3+ at concentration of 1×10-5mol·L-1 significantly increased the alkaline phosphatase activity of ROB cells up to 3 folds (P0.01). However, the effects reversed to inhibit at other concentrations. Gd played a negative role in the alkaline phosphatase activity. La3+ inhibited the adipogenic transdifferentiation of ROB cells at all concentrations in a dose-dependent way. However, Gd promoted the adipogenic transdifferentiation of ROB cells at 1×10 and 1×10 mol·L . These findings suggested that the effects of rare earth elements on the proliferation, differentiation and adipogenic transdifferentiation of ROB cells were dependent on their concentrations and species.