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Effects of Probiotic Lactobacillus gasseri Strains of Breast-Fed Infant in a Murine Model

Journal of Food Science and Engineering 2014年第6期4卷 271-281页

作者： Maria da Penha Piccolo Ramos Joao Alexandre Tres Pancoto Iracilda Zeppone Carlos Rogeria Comastri deCastro Almeida danielle cardoso geraldo maia Livia Carolina de Abreu Ribeiro Femanda Paulin Benzatti Luciana Rodrigues Cunha Aline Costa e Silva Celia Lucia de Luces Fortes Ferreira Departamento de Ciencias da Saude Universidade Federal do Espirito Santo S6o Mateus Espirito Santo CEP. 29.932-540 Brazil Laboratorio de Imunonologia Clinica Universidade Estadual Paulista Faculdade de Ciencias Farmaceuticas Araraquara Sao Paulo CEP: 14.801-902 Brazil Departamento de Ciencia dos Alimentos Universidade Federal da Bahia Eseola de Nutricao Salvador Bahia CEP. 40.110-160 Brazil Departamento de Tecnologia de Alimentos Universidade Federal de Ouro Preto Ouro Preto Minas Gerais CEP: 35.400-970 Brazil Departamento de Tecnologia de Alimentos Universidade Federal de Vigosa Vigosa Minas Gerais CEP: 36.570-000 Brazil

The ingestion of probiotic lactic acid bacteria has been evaluated and noted that it has an effect on the balance of desirable microbiota in the gastrointestinal tract. Lactobacillus gasseri demonstrates good survival in the gastrointestinal tract, and it has been associated with a variety of probiotic activities and roles, including the reduction of fecal mutagenic enzymes, the production of bacteriocins and the stimulation of macrophages immunomodulation. The aim of the study was to evaluate the effects of a pool of L. gasseri strains isolated from the feces of breastfed infants added in the human milk of healthy women. The milk was both pasteurized and unpasteurized, to verify the cell cytotoxicity of macrophages and to quantify the production of immunologic mediators such as IL-4, IL-6, IFN-γ, TNF-α, NO and oxygen intermediary compounds （H2O2）. The administration of raw human milk and pasteurized human milk to infants is a regular, encouraged practice in units of intensive therapy （UITs） and our present investigation verified the beneficial effect of addition of a pool of L. gasseri to pasteurized human milk （PHML）. Our results show that probiotic supplementation helped to maintain cell viability, reduced IL-6 and IFN-γ production and stimulated TNF-α, NO, H2O2, IL-4 production. Nevertheless, the results indicate that the addition of lactobacillus to human milk was not a determinant in the production of TNF-α. L. gasseri added to breast milk did not present a cytotoxic risk, and the addition ofL. gasseri to pasteurized milk of human milk bank would benefit newborns that depend on milk banks for the colonization of more desirable microbiota.

关键词： Probiotic human milk bank immunology Lactobacillus gasseri.

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DNA and histone methylation in gastric carcinogenesis

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World Journal of Gastroenterology 2013年第8期19卷 1182-1192页

作者： danielle Queiroz Calcagno Carolina Oliveira Gigek Elizabeth Suchi Chen Rommel Rodriguez Burbano Marília de Arruda cardoso Smith Department of Morphology and Genetics Federal University of Sao PauloSao PauloSP 04023-900Brazil Laboratory of Human Cytogenetics Institute of Biological SciencesFederal University of ParáBelémPA 66075-110Brazil

Epigenetic alterations contribute significantly to the development and progression of gastric cancer,one of the leading causes of cancer death *** refers to the number of modifications of the chromatin structure that affect gene expression without altering the primary sequence of DNA,and these changes lead to transcriptional activation or silencing of the *** the years,the study of epigenetic processes has increased,and novel therapeutic approaches that target DNA methylation and histone modifications have emerged.A greater understanding of epigenetics and the therapeutic potential of manipulating these processes is necessary for gastric cancer ***,we review recent research on the effects of aberrant DNA and histone methylation on the onset and progression of gastric tumors and the development of compounds that target enzymes that regulate the epigenome.

关键词： Epigenetic DNA methylation Histone methylation Gastric cancer Gastric carcinogenesis

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Metastasis-associated lung adenocarcinoma transcript 1 molecular mechanisms in gastric cancer progression

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World Journal of Gastrointestinal Oncology 2023年第9期15卷 1520-1530页

作者： Daniel Mateus de Oliveira Batista Jessica Manoelli Costa da Silva Carolina de Oliveira Gigek Marilia de Arruda cardoso Smith Paulo Pimentel de Assumpcao danielle Queiroz Calcagno Nucleo de Pesquisas em Oncologia Universidade Federal do ParaBelem 66073-000ParaBrazil Departamento de Patologia Universidade Federal de São PauloSao Paulo 04023-062São PauloBrazil Disciplina de Genetica Departamento de Morfologia e GeneticaUniversidade Federal de São PauloSão Paulo 04023-900Sao PauloBrazil

Gastric cancer(GC)remains among the most common cancers worldwide with a high mortality-to-incidence *** evidence suggests that long noncoding RNAs(lncRNAs)are involved in gastric *** transcripts are longer than 200 nucleotides and modulate gene expression at multiple molecular levels,inducing or inhibiting biological processes and ***-associated lung adenocarcinoma transcript 1(MALAT1)is one of the best-studied lncRNAs with comprehensive actions contributing to cancer *** lncRNA regulates gene expression at the transcriptional and posttranscriptional levels through interactions with microRNAs and *** the present review,we discussed the molecular mechanism of MALAT1 and summarized the current knowledge of its expression in ***,we highlighted the potential use of MALAT1 as a biomarker,including liquid biopsy.

关键词： Long noncoding RNA Gastric carcinogenesis Transcriptional levels Posttranscriptional levels Prognostic biomarker Liquid biopsy

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MYC and gastric adenocarcinoma carcinogenesis

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World Journal of Gastroenterology 2008年第39期14卷 5962-5968页

作者： danielle Queiroz Calcagno Mariana Ferreira Leal Paulo Pimentel Assumpo Marília de Arruda cardoso Smith Rommel Rodríguez Burbano Human Cytogenetics Laboratory Biology Science InstituteFederal University of ParáBelém PA 66075-900Brazil Genetics Division Department of Morphology and GeneticsFederal University of So PauloSo Paulo SP 04023-900Brazil Joo de Barros Barreto University Hospital Federal University of ParáBelém PA 66073-000Brazil

MYC is an oncogene involved in cell cycle regulation, cell growth arrest, cell adhesion, metabolism, ribosome biogenesis, protein synthesis, and mitochondrial function. It has been described as a key element of several carcinogenesis processes in humans. Many studies have shown an association between MYC deregulation and gastric cancer. MYC deregulation is also seen in gastric preneoplastic lesions and thus it may have a role in early gastric carcinogenesis. Several studies have suggested that amplification is the main mechanism of MYC deregulation in gastric cancer. In the present review, we focus on the deregulation of the MYC oncogene in gastric adenocarcinoma carcinogenesis, including its association with Helicobacterpylori （Hpylon] and clinical applications.

关键词： MYC Gastric adenocarcinoma Gastric preneoplastic lesions Gastric carcinogenesis Helicobacter pylori

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Combined Selection in Backcross Population of Papaya(Carica papaya L.)by the Mixed Model Methodology

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American Journal of Plant Sciences 2014年第20期5卷 2973-2983页

作者： Helaine Christine Cancela Ramos Messias Gonzaga Pereira Alexandre Pio Viana Lucas Nunes da Luz Deisy Lucia cardoso geraldo Antonio Ferreguetti Laboratory of Plant Breeding Centre for Agricultural Sciences and TechnologyNorth Fluminense State University(UENF)Campos dos GoytacazesBrazil Caliman Agrícola S.A. LinharesBrazil

The selection of superior genotypes based on the simultaneous response to several characteristics of agronomic importance is a key strategy to overcome the scarcity of available varieties of papaya. This study aimed to apply the combined selection by using distinct selection indexes based on both the genetic values obtained by the REML/BLUP methodology and the real measured values to select agronomically superior genotypes of papaya within backcross progenies. The combined selection was carried out based on genetic and phenotypic values, original and standardized, multiplied by the agronomic weights. The results of the analysis of genetic parameters indicate that the evaluated progenies have expressive genetic variability for the considered traits, and that there are real possibilities of genetic progress with the selection. Among the analyzed indexes, the one based on standardized genetic value presented greater consistency in the ranking of genetic material, demonstrating the advantage of data standardization. Five progenies belonging to the BC1 generation, and five to the BC3 generation were selected using this index. A total of 27 plants ag-ronomically superior were selected within the top five progenies and recommended for generation advance, 23 being selected by combined selection and 4 using the direct selection for the four mainly characters in papaya breeding program: production, pulp and fruit firmness and soluble solids. Beyond the selection of superior genotypes for the development of future inbred lines, this study also allowed defining the best strategy to apply the combined selection in papaya using pre-dicted breeding values obtained by BLUP. This strategy may allow higher accuracy in the selection process, thus increasing the chances of success of the breeding programs.

关键词： Papaya Tree Backcross Selection Index Mixed Model REML/BLUP

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Identification of IL11RA and MELK amplification in gastric cancer by comprehensive genomic profiling of gastric cancer cell lines

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World Journal of Gastroenterology 2016年第43期22卷 9506-9514页

作者： danielle Queiroz Calcagno Sylvia Santomi Takeno Carolina Oliveira Gigek Mariana Ferreira Leal Fernanda Wisnieski Elizabeth Suchi Chen Taíssa Maíra Thomaz Araújo Eleonidas Moura Lima Maria Isabel Melaragno Samia Demachki Paulo Pimentel Assumpcao Rommel Rodriguez Burbano Marília cardoso Smith Disciplina de Genética Departamento de Morfologia e GenéticaUniversidade Federal de Sao Paulo Núcleo de Pesquisas em Oncologia Hospital Universitário Joao de Barros Barreto Departamento de Biologia Molecular Universidade Federal da Paraíba Disciplina de Gastroenterologia Cirúrgica Universidade Federal de Sao Paulo Departamento de Ortopedia e Traumatologia Universidade Federal de Sao Paulo

AIM To identify common copy number alterations on gastric cancer cell *** Four gastric cancer cell lines(ACP02, ACP03, AGP01 and PG100) underwent chromosomal comparative genome hybridization and array comparative genome hybridization. We also confirmed the results by fluorescence in situ hybridization analysis using the bacterial artificial chromosome clone and quantitative real time PCR *** The amplification of 9p13.3 was detected in all cell lines by both methodologies. An increase in the copy number of 9p13.3 was also confirmed by fluorescence in situ hybridization analysis. Moreover, the interleukin 11 receptor alpha(IL11RA) and maternal embryonic leucine zipper kinase(MELK) genes, which are present in the 9p13.3 amplicon, revealed gains of the MELK gene in all the cell lines studied. Additionally, a gain in the copy number of IL11 RA and MELK was observed in 19.1%(13/68) and 55.9%(38/68) of primary gastric adenocarcinoma samples, respectively. CONCLUSION The characterization of a small gain region at 9p13.3 in gastric cancer cell lines and primary gastric adenocarcinoma samples has revealed MELK as a candidate target gene that is possibly related to the development of gastric cancer.

关键词： IL11RA Gastric cancer Genomic profiling MELK 9p13.3

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Clinical implication of 14-3-3 epsilon expression in gastric cancer

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World Journal of Gastroenterology 2012年第13期18卷 1531-1537页

作者： Mariana Ferreira Leal danielle Queiroz Calcagno Smia Demachki Paulo Pimentel Assumpo Roger Chammas Rommel Rodríguez Burbano Marília de Arruda cardoso Smith Genetics Division Department of Morphology and GeneticFederal University of So PauloSo Paulo 04023-900Brazil Pathology Service Joo de Barros Barreto University HospitalFederal University of ParáBelém 60673-000Brazil Surgery Service Joo de Barros Barreto University HospitalFederal University of ParáBelém 60673-000Brazil Laboratory of Experimental Oncology School of MedicineUniversity of So PauloSo Paulo 01246-903Brazil Human Cytogenetics Laboratory Institute of Biological SciencesFederal University of ParáBelém 66073-000Brazil

AIM:To evaluate for the first time the protein and mRNA expression of 14-3-3εin gastric ***:14-3-3εprotein expression was determined by western blotting,and mRNA expression was examined by real-time quantitative RT-PCR in gastric tumors and their matched non-neoplastic gastric tissue ***:Authors observed a significant reduction of 14-3-3εprotein expression in gastric cancer(GC)samples compared to their matched non-neoplastic *** levels of 14-3-3εwere also associated with diffuse-type GC and early-onset of this *** data suggest that reduced 14-3-3εmay have a role in gastric carcinogenesis ***:Our results reveal that the reduced 14-3-3εexpression in GC and investigation of 14-3-3ε interaction partners may help to elucidate the carcino-genesis process.

关键词： Gastric cancer 14-3-3 epsilon YWHAE Geneexpression Protein expression

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Synthesis, Characterization, and Study of PLGA Copolymer <i>in Vitro</i>Degradation

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Journal of Biomaterials and Nanobiotechnology 2015年第1期6卷 8-19页

作者： Anamaria Teodora Coêlho Rios Silva Barbara Camilla Oliveira cardoso Maria Elisa Scarpelli Ribeiro e Silva Roberto Fernando Souza Freitas Ricardo geraldo Sousa Polymer Science and Technology Laboratory Chemical Engineering Department School of Engineering Federal University of Minas Gerais Belo Horizonte Brazil

The poly(lactic-co-glycolic acid), known as PLGA, is one of the main bioreabsorbable polymers used in the field of medicine today. This copolymer is widely applied in sutures, devices geared toward the controlled release of medication, and the guided regeneration of bone tissue as it presents a short degradation time. This work aimed to synthesize the 82/18 PLGA (expressed by the mass ratio of D,L-lactide and glycolide, respectively), to characterize and study the in Vitro degradation in the form of rods in phosphate buffer solution (PBS). The copolymer was synthesized by opening the cyclic dimer rings of the monomers D,L-lactide and glycolide, in the presence of the tin octanoate initiator and of the lauryl alcohol co-initiator. The characterization of the copolymer and the follow-up of its in vitro degradation were studied using: Differential Scanning Calorimetry (DSC), Thermogravimetry (TG), Infrared Molecular Absorption Spectroscopy with Fourier Transform (FTIR), Rheometry, and Scanning Electron Microscopy (SEM). Through these characterization techniques, it was possible to obtain the glass transition temperature, thermal stability, chemical composition, morphology, and molar mass of both the synthesized and the degraded copolymer. The molar mass of the synthesized copolymer was, approximately, 106 g·mol-1. The degradation rate of PLGA significantly increased from the 19th to the 28th day in PBS. After 28 days in PBS, the glass transition temperature and the molar mass reduced from 45°C to 17°C and from 1.5 × 106 g·mol-1?to 7.5 × 104?g·mol-1, respectively. The pH of the medium has a significant influence on the copolymer degradation profile. When it diminishes, it accelerates the degradation process, resulting in smaller PLGA polymer chains. This pH dependent degradation can be useful for drug release systems.

关键词： Synthesis Characterization In Vitro Degradation PLGA

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An Emergy-Based Approach to Assess and Valuate Ecosystem Services of Tropical Wetland Pastures in Brazil

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Open Journal of Ecology 2020年第5期10卷 303-319页

作者： Sandra Aparecida Santos Fabio Takahashi Evaldo Luis cardoso Claudio Flores Luiz Orcirio Fialho de Oliveira geraldo da Silva e Souza Eliane Goncalves Gomes Enrique Ortega Embrapa Pantanal CorumbaMSBrasil Universidade de Vicosa FlorestalMGBrasil Embrapa Gado de Corte Campo GrandeMSBrasil Embrapa Sede BrasiliaDFBrasil Universidade de Campinas Barao GeraldoSPBrasil

Wetland grasslands are important ecosystems for raising beef cattle, because they are highly productive and present forages with high quality. Most of these ecosystems are threatened by overgrazing or by being replaced by exotic pastures. Emergy synthesis approach was used to assess and value the services provided by native pastures wetland under three conservation status and also to compare them to exotic pastures on wetlands. The ecosystem service that was assessed included forage provision for calves production estimated from grazing capacity of cow with calf at the foot. Habitat maintenance to plant diversity and wild herbivores were also evaluated. The results showed that natural wetland pastures with better conservation status provided valuable ecosystem services and are highly renewable. Data envelopment analysis (DEA) showed that regardless of the conservation state of the natural pastures, wet native grasslands were more efficient than wet exotic grasslands. Replacing native pastures by exotic ones may reduce plant diversity and the renewability of the system. The proposed method has a holistic approach to pasture ecosystems and is able to help decision-makers to define sustainable management practices and to subsidise public policies when it comes to payments regarding ecosystem services.

关键词： Data Envelopment Analysis Forage Resources Natural Pasture Plant Diversity Sustainability

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Gastric Bypass with Long Pouch and Transit Bipartition for Endoscopic Access to the Remaining Stomach

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Surgical Science 2022年第8期13卷 353-366页

作者： Paulo Reis Esselin de Melo Marcella Giovana Gava-Brandolis Thonya Cruz Braga Danilo Fossalussa Minari Ricardo Augusto Martins Bueno da Costa Jose geraldo Moraes Sampaio Neto Louise Flores Silva José Humberto cardoso Resende Hospital Estadual Alberto Rassi Surgical Skills Coordinator Goi&#226nia Brazil UNIFAN Research Coordinator Aparecida de Goi&#226nia Brazil

Introduction: obesity has a complex and multifactorial etiology, difficult treatment and increasing incidence rates in recent decades. The treatment involves clinical and pharmacological approaches and, in case of lack of results, surgical interventions. Roux-en-Y gastric bypass (RYGB) is one of these surgical interventions in which the stomach is divided, creating a small pouch, and the remaining portion of the stomach become excluded and left without endoscopic access. Objective: to evaluate the results of modified RYGB with long pouch and endoscopic access to the remaining stomach. Materials and Methods: prospective clinical trial with sample selected among patients seen at the Alberto Rassi General State Hospital of Goiânia (HGG) and indicated for bariatric and metabolic surgery confirmed by the medical and multidisciplinary team. The study was conducted from January 2020 to August 2021. Clinical history and laboratory test results of the selected patients were collected through consultations with the medical and multidisciplinary team. Results: twelve participants were included in the study. Of these, 11 (91.7%) were women and the mean age was 46.3 years. The weight before surgery was 112.17 kg (92.00 - 150.00) and the Body Mass Index (BMI) was 44.89 (35.06 - 74.39). After 18 months of surgery, the mean weight was 80.77 kg (±11.92) and the mean BMI was 29.46 (±11.00), showing a significant reduction in both (p = 0.003 and p = 0.002, respectively). All patients underwent endoscopic evaluation of the pouch, remaining stomach and duodenum at 12 months postoperatively. The mean percentage of lost excess weight loss was 68.21%. Conclusion: We conclude that the proposed changes in RYGB (GBLP + GIB – Roux-en-Y gastric bypass with long pouch and gastrointestinal bipartition) did not compromise weight loss or control of type 2 diabetes and other comorbidities and proved to be a safe and effective alternative without gastroduodenal exclusion, enabling a better postop

关键词： Obesity Bariatric Surgery Metabolic Surgery Roux-En-Y Gastric Bypass

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