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Dual role of FGF in proliferation and endoreplication of Drosophila tracheal adult progenitor cells

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Journal of Molecular Cell Biology 2020年第1期12卷 32-41页

作者： cristina de Miguel Josefa cruz David Martin Xavier Franch-Marro Institute of Evolutionary Biology(Consejo Superior de Investigaciones Cientificas-Universitat Pompeu Fabra) Passeig Maritim de la Barceloneta 3708003 BarcelonaSpain

Adult progenitor cells activation is a key event in the formation of adult *** Drosophilat formation of abdominal adult trachea depends on the specific activation of tracheal adult progenitors(tracheoblasts)at the Tr4 and Tr5 spiracular *** of these tracheoblasts generates a pool of tracheal cells that migrate toward the posterior part of the trachea by the activation of the branchless/fibroblast growth factor(Bnl/FGF)signaling to form the abdominal adult ***,we show that,in addition to migration,Bnl/FGF signaling,mediated by the transcription factor Pointed,is also required for tracheoblast *** tracheoblast activation relies on the expression of the FGF ligand bnl in their nearby ***,we show that,in the absence of the transcription factor Cut(Ct),Bnl/FGF signaling induces endoreplication of tracheoblasts partially by promoting fizzy-related ***,our results suggest a dual role of Bnl/FGF signaling in tracheoblasts,inducing both proliferation and endoreplication,depending on the presence or absence of the transcription factor Ct,respectively.

关键词： FGF trachea adult progenitors cut proliferation endoreplication Pnt Fzr

Biochar influences the cane fields’microbiota and the development of pre-sprouted sugarcane seedlings

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Waste Disposal and Sustainable Energy 2023年第1期5卷 75-88页

作者： Bruna cristina Pinto Adriana Barboza Alves Osania Emerenciano ferreira Gustavo Henrique Gravatim Costa Magno Andréde oliveira Augusto Cesar da Silva Bezerra Alan Rodrigues Teixeira Machado Postgraduate Program in Environmental Sciences State University of Minas GeraisFrutal UnityFrutalMinas Gerais38202-436Brazil department of Agricultural and Biological Sciences State University of Minas GeraisFrutal UnityFrutalMinas Gerais38208-436Brazil Postgraduate Program in Geotechnical Engineering Federal University of Ouro PretoOuro PretoMinas Gerais35400-000Brazil department of Transport Engineering Federal Center for Technological Education of Minas GeraisBelo HorizonteMinas Gerais30421-169Brazil department of Exact Sciences State University of Minas GeraisJoão Monlevade UnityJoão MonlevadeMinas Gerais35930-314Brazil

This research investigates the soil conditioning effect of sugar cane fields with biochar produced from sugarcane *** influence on the physicochemical and microbiological conditions of the soil and the agronomic performance of sugarcane seedlings was *** this end,the biochar from sugarcane bagasse was produced by pyrolysis in a double drum oven and mixed with soil at rates of 0,1%,3%,and 5%(in weight).The experiment was installed in a greenhouse with two evaluation periods(30 days and 60 days)and five *** pot received a pre-sprouted cane seedling,and the analysis of colony-forming units(bacteria and fungi),physicochemical characteristics of the soil,and growth and development of sugarcane were performed per *** biochar showed a high fixed carbon content(72%)and a specific surface area estimated by the adsorption of methylene blue of 50 m2 g−***,its structure is porous and contains important nutrients(e.g.,CaO,K2O,and P2O5).In the periods evaluated,the applications of the 3%and 5%(in weight)rates of biochar reduced most of the soil fertility ***,the chemical analyses indicated that the sugarcane field soil collected already possessed high *** microbiota was influenced,but only the application of the 3%(in weight)rate at 60 days after planting showed a significant positive effect on the number of bacteria forming units,with an increase of approximately 385%.On the other hand,no significant positive effect on sugarcane development was ***,biochar application in high-fertility sugarcane plantation soil did not result in gains for sugarcane development in the periods evaluated.

关键词： Soil Biometry Microbiology Double drum oven Biochar

Different culture conditions applied to in vitro shoot multiplication of two Eucalyptus benthamii explant sources

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Journal of Forestry Research 2020年第3期31卷 857-869页

作者： Natalia Pimentel Esposito-Polesi Leandro Silva de oliveira Francisco Jose Benedini Baccarin cristina Vieira de Almeida Marcilio de Almeida Biological Science department "Luiz de Queiroz"Superior College of AgricultureUniversity of Sao Paulo(ESALQ/USP)Padua Dias Avenue11P.O.Box 9PiracicabaSP 13418-900Brazil Institute of Agriculture Sciences Federal University of Minas Gerais(UFMG)Universitaria Avenue1000Montes ClarosMG 39404-547Brazil State University of Goias(UEG) Rua S7Qd.1S SulPalmeiras de GoiasGO 76190-000Brazil In vitro Palm Consultoria Estudo e Desenvolvimento Biologico LtdaRua Itajobi421PiracicabaSP 13432-000Brazil

Eucalyptus adult material requires more successive subcultures in the in vitro multiplication phase for increased vigor and cellular activity. This study evaluated the endophytic manifestation and shoot multiplication of one 13-year-old Eucalyptus benthamii clone under different culture conditions and used canopy branches(CB) and trunk base material as explant sources. The culture media were wood plant medium(WPM), Murashige and Skoog medium(MS) and JADS(Correia and co-authors medium).Based on the results of the initial multiplication experiment, further tests examined sucrose concentrations and p H. Morphophysiology, dry mass production, endophyticmanifestation and histochemical were determined. Explant sources responded differently to MS and JADS media, but the WPM medium promoted homogeneous *** responses were similar for both explant sources when sucrose concentrations varied. Shoots died in the absence of sucrose, showed high oxidation at 60 g L-1 and optimal development at 30 g L-1. Endophytes were more evident for shoots from the CB origin. Explant sources responded distinctively to treatment due to physiological and intrinsic genetic factors. Therefore, explant sources, different culture media, sucrose concentration and p H may determine micropropagation success and influence the presence and/or intensity of endophytic manifestation.

关键词： Plant tissue culture Explant sources Culture media Sucrose pH

Fraud investigation in commercial coffee by chromatography

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Food Quality and Safety 2018年第3期2卷 121-133页

作者： Víctor de Carvalho Martins Ronoel Luiz de oliveira Godoy Ana cristina Miranda Senna Gouvêa Manuela cristina Pessanha de Araujo Santiago Renata Galhardo Borguini Elaine cristina de oliveira Braga Sidney Pacheco Luzimar da Silva de Mattos do Nascimento departamento de Tecnologia de Alimentos Instituto de TecnologiaUniversidade Federal Rural do Rio de JaneiroCEP 23890-000Seropédica-RJBrazil Laboratório de Cromatografia Líquida Embrapa Agroindústria de AlimentosCEP 23020-470Rio de Janeiro-RJBrazil Centro de Tecnologia Instituto de QuímicaUniversidade Federal do Rio de JaneiroCEP 22290-180Rio de Janeiro-RJBrazil

Coffee is currently the second largest commodity on the world market today,and there is great concern about the quality of the beans exported from producer countries to Europe and *** such as using blends of different species and adding low-cost raw materials,such as chicory,corn,and soybean,impair the sensory and functional characteristics of the drink made from roasted and ground coffee *** is a need to adopt more efficient analytical methods than the microscopy technique currently *** first chromatographic method used to determine fraud was reported in *** method used paper chromatography to differentiate between coffee and chicory based on the free reducing *** of the 1980s,different methods involving high-performance liquid chromatography and gas chromatography were developed in order to demonstrate geographic authenticity,distinction between species,occurrence of adulteration,and the presence of defective beans by determining the monosaccharides,oligosaccharides,tocopherols,fatty acids,volatiles,diterpenes,sterols,and phenolic substances,among *** far as the authors know,there are no papers published in the literature that have compiled such an extensive set of information about these chromatographic methods as *** the last 2 years,there has been a trend to develop analytical methods for ultra-performance liquid chromatography coupled with tandem mass spectrometry to confirm fraud in coffee,due to high sensitivity and selectivity.

关键词： adulteration coffee chromatographic methods carbohydrates volatile substances

A review on the synergism between corrosion and fatigue of magnesium alloys:Mechanisms and processes on the micro-scale

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Journal of Magnesium and Alloys 2024年第8期12卷 3062-3093页

作者： Mara cristina Lopes de oliveira Rejane Maria Pereira da Silva Ricardo M.Souto Renato Altobelli Antunes Federal University of the ABC Center for EngineeringModeling and Applied Social SciencesAv.Dos Estados500109280-560 Santo AndréSPBrazil department of Chemistry-PPGQ State University of PiauíUESPICampus Poeta Torquato NetoPirajáTeresina 64002-150 PIBrazil Institute of Material Science and Nanotechnology University of La LagunaP.O.Box 45638200 La LagunaTenerifeSpain

Understanding the interaction between cyclic stresses and corrosion of magnesium(Mg)and its alloys is increasingly in demand due to the continuous expansion of structural applications of these *** review is dedicated to exploring the corrosion-fatigue mechanisms of these materials,with an emphasis on microscale processes,and the possibility of expanding current knowledge on this topic using scanning electrochemical *** interaction between fatigue and corrosion of Mg alloys is analyzed by considering the microstructural aspects(grain size,precipitates,deformation twins),as well as the formation of ***,in the case of coated alloys,the role of coating defects in these phenomena is also *** this context,the feasibility of using scanning electrochemical microscopy(SECM),scanning vibrating electrode technique(SVET),scanning ion-selective electrode technique(SIET),localized electrochemical impedance spectroscopy(LEIS)and scanning Kelvin probe(SKP)methods to study the corrosion-fatigue interaction of Mg alloys is examined.A comprehensive review of the current literature in this field is presented,and the opportunities and limitations of consolidating the use of these techniques to study the microscale processes involved in Mg corrosion-fatigue are discussed.

关键词： Magnesium alloys Localized corrosion and protection Fatigue Scanning electrochemical microscopy Scanning vibrating electrode technique Scanning ion-selective electrode technique Scanning Kelvin probe Chemical imaging

Vitellogenin content in fat body and ovary homogenates of workers and queens of Melipona quadrifasciata anthidioides during vitellogenesis

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Insect Science 2012年第2期19卷 213-219页

作者： Vagner Tadeu Paes de oliveira Bruno Berger Carminda da cruz-Landim Zila Luz Paulino Simoes departamento de Biologia Instituto de Biociencias de Rio ClaroUNESP-Univ.Bela VistaRio ClaroSao Paulo departamento de Biologia FFFL de Ribeirao PretoUniversidade de Sao PauloUSPSao PauloBrazil

Vitellogenin （Vg） is an egg yolk protein that is produced primarily in the fat body of most female insects. In the advanced social structure of eusocial honeybees, the presence of the queen inhibits egg maturation in the workers＇ ovaries. However in the stingless bee Melipona quadrifasciata, the workers always develop ovaries and lay a certain amount of eggs while provisioning the brood cells with larval food during what is known as the worker nurse phase. The present work is a comparative study of the presence of Vg in homogenates of the fat bodies and ovaries of the nurse workers, and the virgin and physogastric queens of M. quadrifasciata. The presence of Vg was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis and irnmunoblotting using Apis mellifera anti-egg antibody. Vg was not detected in the fat bodies or ovaries of the workers, but it was found in the ovaries of virgin and physogastric queens and in the fat body ofphysogastric queens. The results are discussed, taking into account the reproductive state of the individuals and the other possible roles of Vg, such as a storage protein for metoabolism of other organs.

关键词： egg yolk electrophoresis immunoblotting nurse worker stingless bee virginand physogastric queen

Friction ridge analysis in disaster victim identification(DVI):Brazilian case studies

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Forensic Sciences Research 2022年第2期7卷 323-329页

作者： Marco Antonio de Souza Gabriel de oliveira Urtiaga Renata cristina Grangeiro ferreira Luciene Marques da Silva Jade Kende Gonçalves Umbelino Flávio Roberto de Melo Simone de Jesus Instituto Nacional de Identificação Polícia FederalBrasíliaBrazil Grupo de Identificação Superintendência Regional da Polícia FederalPorto AlegreBrazil departamento de Polícia Técnica Polícia Civil do Distrito FederalBrasíliaBrazil Núcleo de Identificação Superintendência Regional de Polícia FederalBelo HorizonteBrazil Instituto de Identificação Polícia CivilGoiâniaBrazil

depending on the magnitude and nature of a disaster,identifying the victims can be a complex task that requires coordinated work by disaster victim identification(DVI)teams based on pre-established ***,the analysis of fingerprints has been presented as a method to establish,when possible,the identity of the victims during the DVI *** study discusses the importance of this primary method of identification and the results obtained in four different disasters in which Brazilian DVI teams were involved:the Air France Flight AF447 plane crash in the Atlantic Ocean,floods and mudslides in the State of Rio de Janeiro,Brazil,the LaMia Flight 2933 plane crash in Colombia,and the tailings dam collapse in Brumadinho,***,we also report the use of the automatic fingerprint capture and identification system,called Alethia,developed by the Federal Police of Brazil and used in the victim identification process in the two latter events mentioned above.

关键词： Forensic sciences disaster victim identification DVI fingerprint ridgeology Alethia System

Effects of Soluble Silicate and Nanosilica Application on Rice Nutrition in an Oxisol

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Pedosphere 2018年第4期28卷 597-606页

作者： Rita de Cassia FELIX ALVAREZ Renato de Mello PRADO Guilherme FELISBERTO Angelica cristina FERNANdeS deUS Raimundo Leonardo LIMA de oliveira Federal University of Mato Grosso do Sul Chapadao do Sul 79560-000 (Brazil) University of the State of Sao Paulo UNESP School of Agronomic Sciences Department of Soils and Fertilizers Jaboticabal 14884-900 (Brazil)

Silicon （Si） has been supplied to plants via application of calcium silicate to soil; however, high doses of calcium silicate are required because of its low solubility. Nanoparticles can reduce Si doses and be applied to seeding furrows. This study investigated the effects of liquid Si sources, i.e., highly soluble silicate （115.2 g L^-1 Si and 60.5 g L^-1 Na20） and nanosilica （〈 200 nm）, on Si uptake by rice plants, plant lignification, plant C：N：P stoichiometry, plant physiology, and grain yield using an Oxisol under greanhouse condistions. The treatments included the application of nanosilica and soluble silicate to seeding furrows at Si doses of 0, 605, 1210, and 2 420 g ha^-1. Plant uptake and treatment effects were evaluated by measuring C and lignin contents, Si, N, and P accumulation, physiological characteristics, and grain yield of rice. The deposition of silica bodies and amorphous silica in the flag leaves was analyzed using scanning electron microscopy. Application of liquid Si increased Si accumulation in rice by 47.3% in relation to the control （0 g ha^-1 Si）, regardless of the Si sources used. Nanosilica application increased leaf lignin content by 112.7% when compared to that in the control. Silicon moderately affected the net C assimilation （increased by 1.83%） and transpiration rates （increased by 48.3%）; however, Si influenced neither plant growth nor grain yield of rice. These results are explained by the lack of biotic or abiotic stress in rice plants during the experiment. To the best of our knowledge, in Brazilian agriculture, this is the first report on the use of nanosilica as a Si fertilizer and its effect on plant nutrition. This study provides evidence that rice plants absorb and accumulate nanoparticles; however, further studies are required to investigate the use of nanoparticles in other plant species.

关键词： C:N:P stoichiometry grain yield leaf lignin net C assimilation plant physiology silicon source

Investigating local corrosion processes of magnesium alloys with scanning probe electrochemical techniques: A review

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Journal of Magnesium and Alloys 2022年第11期10卷 2997-3030页

作者： Mara cristina Lopes de oliveira Rejane Maria Pereira da Silva Ricardo M.Souto Renato Altobelli Antunes Federal University of ABC Center for EngineeringModeling and Applied Social SciencesAv.Dos Estados500109210-580Santo AndréSP Brazil Instituto de Pesquisas Energéticas e Nucleares IPEN/CNENAv.Prof.Lineu Prestes2242São PauloBrazil Institute of Material Science and Nanotechnology University of La LagunaP.O.Box 456La LagunaTenerife 38200Spain

The study of corrosion of magnesium and its alloys has emerged a hot topic in the applications of lightweight structural materials. The inherently high electrochemical activity of bare magnesium surfaces still lacks a convincing mechanism to describe the observed experimental characteristics, and it has prompted the development of various types of protective coatings with the aim of slowing metal dissolution. In recent years, new instruments and techniques have been developed to study with spatial resolution the local corrosion processes that occur in metallic materials in general, and for magnesium and its alloys in particular, both for bare surfaces and coated. Scanning microelectrochemical techniques, such as local electrochemical impedance spectroscopy(LEIS), scanning electrochemical microscopy(SECM), scanning vibrating electrode technique(SVET), scanning ion-selective electrode technique(SIET) and scanning Kelvin probe(SKP) can provide information about the local electrochemical activity of metallic surfaces. In the present work, the applications of these techniques in corrosion studies of magnesium and its alloys are reviewed. Assessment of corrosion mechanisms, barrier properties of conventional coatings and active corrosion behavior of self-healing coatings are examined. Limitations and future developments in this area are discussed.

关键词： Magnesium alloys LEIS SECM SVET SIET SKP