Different culture conditions applied to in vitro shoot multiplication of two Eucalyptus benthamii explant sources

作     者：Natalia Pimentel Esposito-Polesi Leandro Silva de Oliveira Francisco Jose Benedini Baccarin Cristina Vieira de Almeida Marcilio de Almeida Natalia Pimentel Esposito-Polesi;Leandro Silva de Oliveira;Francisco José Benedini Baccarin;Cristina Vieira de Almeida;Marc?élio de Almeida

作者机构：Biological Science Department"Luiz de Queiroz"Superior College of AgricultureUniversity of Sao Paulo(ESALQ/USP)Padua Dias Avenue11P.O.Box 9PiracicabaSP 13418-900Brazil Institute of Agriculture SciencesFederal University of Minas Gerais(UFMG)Universitaria Avenue1000Montes ClarosMG 39404-547Brazil State University of Goias(UEG)Rua S7Qd.1S SulPalmeiras de GoiasGO 76190-000Brazil In vitro Palm ConsultoriaEstudo e Desenvolvimento Biologico LtdaRua Itajobi421PiracicabaSP 13432-000Brazil 

出 版 物：《Journal of Forestry Research》 (林业研究（英文版）)

年 卷 期：2020年第31卷第3期

页      面：857-869页

核心收录：

学科分类：0907[农学-林学] 08[工学] 0829[工学-林业工程] 09[农学] 

基　　金：supported by the National Council of Technological and Scientific Development(CNPq)Process No.143253/2011-5 to Coordination for the Improvement of Higher Education Personnel(CAPES) 

主　　题：Plant tissue culture Explant sources Culture media Sucrose pH 

摘      要：Eucalyptus adult material requires more successive subcultures in the in vitro multiplication phase for increased vigor and cellular activity. This study evaluated the endophytic manifestation and shoot multiplication of one 13-year-old Eucalyptus benthamii clone under different culture conditions and used canopy branches(CB) and trunk base material as explant sources. The culture media were wood plant medium(WPM), Murashige and Skoog medium(MS) and JADS(Correia and co-authors medium).Based on the results of the initial multiplication experiment, further tests examined sucrose concentrations and p H. Morphophysiology, dry mass production, endophyticmanifestation and histochemical were determined. Explant sources responded differently to MS and JADS media, but the WPM medium promoted homogeneous *** responses were similar for both explant sources when sucrose concentrations varied. Shoots died in the absence of sucrose, showed high oxidation at 60 g L-1 and optimal development at 30 g L-1. Endophytes were more evident for shoots from the CB origin. Explant sources responded distinctively to treatment due to physiological and intrinsic genetic factors. Therefore, explant sources, different culture media, sucrose concentration and p H may determine micropropagation success and influence the presence and/or intensity of endophytic manifestation.