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Each Epiphyte on Its Branch: A Comparative Study between Different Phorophytes

Agricultural Sciences 2023年第5期14卷 636-644页

作者： Johnatan Jair de Paula Marchiori Vinicius de Souza oliveira Eduarda Carriço Ana Clara Bayer Bernabé Anderson Mathias Holtz Ronilda Lana Aguiar Ana Beatriz Mamedes Piffer Gilcéa Teixeira Fontana Boone Lusiane de Sousa Ferreira bruna de oliveira magnani Federal University Rural of Rio de Janeiro Seropédica Brazil Center for Agricultural Sciences and Engineering Federal University of Espírito Santo Alegre Brazil Federal Institute of Espírito Santo Campus Itapina Colatina Brazil State University Paulista “Júlio de Mesquita Filho” Botucatu Brazil

Epiphytes are plants that use the mechanical support provided by a host tree, called phorophytes, without emitting haustorial structures. Constituting an important component of the flora, playing an important role in the maintenance of ecosystems, such as water and nutrient cycling, in addition to providing resources, sometimes unique, such as food and shelter, for the canopy fauna. However, the epiphytic flora may show high beta diversity, even when compared with different phorophytes, possibly as a response to the dendromorphological characteristics of the phorophyte. Thus, this work aims to: 1) compare whether the species richness of epiphytes differs in trees with different morphological characteristics, and 2) compare species composition among three tree species. For this, 10 individuals of three tree species were selected: Guaria guidonia, Ficus sp., and Roystonea oleracea. In each individual, the number of species (richness) of vascular epiphytes was recorded and obtained from the species composition. To test differences in richness values between the three trees, we used analysis of variance (ANOVA) followed by Tukey’s test. To understand whether the epiphyte community formed separate groups according to the tree species used, we used the available non-metric multidimensional scaling method (NMDS). Thirty-four species of epiphytes were found in the 30 phorophytes surveyed. The three tree species showed significantly different richness, with the highest found in Guarea guidonea and the lowest in Roystonea oleraceae. The NMDS showed that epiphytes group the three tree species into distinct groups, although Ficus sp individuals present a transitional community between the two other species. Thus, the results suggest that the differences in the richness and composition of the epiphytic flora of the different phorophytes are influenced by their distinct morphological characteristics.

关键词： Atlantic Forest Ecological Reserve Tree Species

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Palm Red Mite Management with Soursop Seed Plant Residue Extracts

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Agricultural Sciences 2023年第4期14卷 541-552页

作者： Ana Beatriz Mamedes Piffer Evellyn Zuqui Bolsoni Isabella Merlo Fornaciari Anderson Mathias Holtz Johnatan Jair de Paula Marchiori Jose Romário de Carvalho Ronilda Lana Aguiar Patrícia Soares Furno Fontes bruna de oliveira magnani Campus Itapina Federal Institute of Espírito Santo Colatina Brazil Campus Seropédica Federal University Rural of Rio de Janeiro Seropédica Brazil Secretary of State for Education of Espírito Santo Gua&#231uí Brazil

Raoiella indica Hirst, 1924 (Prostigmata: Tenuipalpidae), is one of the leading pest mites in palm and banana trees, however, there are few control methods available for this pest species. Therefore, this work aimed to evaluate the acaricidal effect of soursop seed extract (Annona muricata L.) on R. indica adults. The experiment was conducted in a completely randomized design using soursop seed extract with 7 replicates and 12 individuals of R. indica per replicate. The experimental units consisted of discs of coconut palm leaves (4 cm in diameter), with cotton moistened at the bottom of the Petri dish (10.0 × 1.2 cm) and around the disc to maintain turgor and prevent mites from escaping. The application was performed using an airbrush, connected to a calibrated compressor with a constant pressure of 1.3 psi and 1 mL of solution per repeat plate. The acaricidal effect was evaluated 24, 48, and 72 hours after spraying. Mortality data were corrected and submitted to Probit analysis (p ≤ 0.05) using the statistical program R, with the LC50 and LC90 calculated for the extract. At the maximum concentration (15%), the soursop seed extract showed mortality of 70% of individuals of R. indica, and the LC50 was 6.58%. It was concluded that the soursop seed extract showed acaricidal potential on R. indica in the laboratory.

关键词： Annona muricata Raoiella indica Alternative Control Agro-Industrial Residue

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Biochar influences the cane fields’microbiota and the development of pre-sprouted sugarcane seedlings

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Waste Disposal and Sustainable Energy 2023年第1期5卷 75-88页

作者： bruna Cristina Pinto Adriana Barboza Alves Osania Emerenciano Ferreira Gustavo Henrique Gravatim Costa Magno Andréde oliveira Augusto Cesar da Silva Bezerra Alan Rodrigues Teixeira Machado Postgraduate Program in Environmental Sciences State University of Minas GeraisFrutal UnityFrutalMinas Gerais38202-436Brazil department of Agricultural and Biological Sciences State University of Minas GeraisFrutal UnityFrutalMinas Gerais38208-436Brazil Postgraduate Program in Geotechnical Engineering Federal University of Ouro PretoOuro PretoMinas Gerais35400-000Brazil department of Transport Engineering Federal Center for Technological Education of Minas GeraisBelo HorizonteMinas Gerais30421-169Brazil department of Exact Sciences State University of Minas GeraisJoão Monlevade UnityJoão MonlevadeMinas Gerais35930-314Brazil

This research investigates the soil conditioning effect of sugar cane fields with biochar produced from sugarcane *** influence on the physicochemical and microbiological conditions of the soil and the agronomic performance of sugarcane seedlings was *** this end,the biochar from sugarcane bagasse was produced by pyrolysis in a double drum oven and mixed with soil at rates of 0,1%,3%,and 5%(in weight).The experiment was installed in a greenhouse with two evaluation periods(30 days and 60 days)and five *** pot received a pre-sprouted cane seedling,and the analysis of colony-forming units(bacteria and fungi),physicochemical characteristics of the soil,and growth and development of sugarcane were performed per *** biochar showed a high fixed carbon content(72%)and a specific surface area estimated by the adsorption of methylene blue of 50 m2 g−***,its structure is porous and contains important nutrients(e.g.,CaO,K2O,and P2O5).In the periods evaluated,the applications of the 3%and 5%(in weight)rates of biochar reduced most of the soil fertility ***,the chemical analyses indicated that the sugarcane field soil collected already possessed high *** microbiota was influenced,but only the application of the 3%(in weight)rate at 60 days after planting showed a significant positive effect on the number of bacteria forming units,with an increase of approximately 385%.On the other hand,no significant positive effect on sugarcane development was ***,biochar application in high-fertility sugarcane plantation soil did not result in gains for sugarcane development in the periods evaluated.

关键词： Soil Biometry Microbiology Double drum oven Biochar

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Anaerobic Digestion of Food Waste Using UASB Sludge from a Poultry Slaughterhouse as Inoculum

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Journal of Agricultural Science and Technology(A) 2017年第7期7卷 507-512页

作者： bruna Patricia de oliveira Luisa Helena dos Santos oliveira Gilberto Martins Programa de Pos Graduacao em Energia Universidade Federal do ABC Santo Andre 09210-580 Brazil Programa de Pos Graduacao em Ciencia e Tecnologia Ambiental Universidade Federal do ABC Santo Andre 09210-580 Brazil

In 2010, Brazil endorsed a new national policy of solid wastes, which has a very ambitious target to close up all the dumping areas and to manage adequately all the municipal solid waste, disposing in landfills only the refuse of treated municipal solid waste after 2019. Food waste is the largest representative of organic waste produced in Brazilian municipalities, which can produce both fertilizer and biogas through anaerobic digestion （AD） process, reducing in both cases greenhouse gases emissions. AD assays of some samples of restaurant food wastes were performed in batch laboratory, 500 mL reactors for determination of optimized start-up conditions. The tests were conducted in triplicate, using anaerobic sludge from a poultry slaughterhouse wastewater treatment as inoculum to start up the anaerobic biodigestors. The effect of three different substrates to inoculum mixing ratios 10：90 （S1）, 20：80 （S2） and 30：70 （S3） was evaluated. The food waste used as substrate was previously homogenized and submitted to aerobic mechanical mixing for four days before it was fed in the reactors in anaerobic conditions at the mentioned proportions with the inoculum. The reactors were maintained at 35 ℃ and under mechanical mixing at 60 rpm. The methane production was monitored until its stabilization. Results showed that S1 presented the best performance during the initial 12 d, when it totalized 526 NmLCH4/gVSsubstrate.

关键词： Biogas methane food waste inoculum poultry sludge anaerobic biodigestion.

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Proposal for the Adaptation of Brazilian Standard ABNT NBR 16274 for Correct Assessment of PV Generator Connected to the Grid with Microinverter

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Journal of Energy and Power Engineering 2018年第12期12卷 591-598页

作者： Felipe de oliveira Rodrigo Teixeira Tatiani Pivem Sandro Petry bruna Larissa Spontoni Laura de oliveira The department of Research and development Nexsolar Solu珲es em Energia Solar LTDACampo GrandeCEP:79002-450Brazil The department of Civil Engineering Universidade Federal de Mato Grosso do SulCampo GrandeCEP:79070-00Brazil

One of the main promises of solar photovoltaic technology has been the use of so-called microinverter which intends to make it possible for anyone to produce their own power *** article presents a discussion of a proposal to adapt the Brazilian Standard NBR 16274(photovoltaic systems connected to the network—minimum requirements for documentation,commissioning tests,inspection and performance evaluation)of grid-connected photovoltaic(PV)system design methodology-oriented inverter module(or microinverters).The article begins by presenting the different methodologies grid-connected PV system projects discussing positive and negative aspects of *** search presents a discussion of the positive and negative aspects of grid-connected PV system design methodology with microinverters,giving a practical example of *** paper is organized as follows:Section 1 provides an introduction explaining the importance of the proposed methodology;Section 2 presents the different types of grid-connected PV system design methodology as well as its positive and negative aspects;Section 3 describes the key differentiators of grid-connected PV system design methodology with use of microinverters finished with practical example of scaling;Section 4 presents the main proposals to adapt and points on conflict of NBR 16274;Section 5 presents the work’s conclusions.

关键词： Solar energy Brazilian standard microinverter

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Candida albicans isolated from urine:Phenotypic and molecular identification,virulence factors and antifungal susceptibility

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Asian Pacific Journal of Tropical Biomedicine 2017年第7期7卷 624-628页

作者： Laura Wiebusch Adriana Araújo de Almeida-Apolonio Luana Mireli Carbonera Rodrigues bruna de Paula Bicudo Danielly Beraldo dos Santos Silva Danielle Ferreira Lonchiati Renata Pires de Araujo Alexéia Barufatti Grisolia Kelly Mari Pires de oliveira Faculdade de Ciencias Biologicas e Ambientais Universidade Federal da Grande DouradosDouradosMSBrazil Faculdade de Medicina Universidade Federal de Mato Grosso do SulCampo GrandeMSBrazil Faculdade de Ciencias da Saude Universidade Federal da Grande DouradosDouradosMSBrazil Faculdade de Ciencias Agrarias e Veterinarias Universidade Estadual Paulista“Julio de Mesquita Filho”JaboticabalSPBrazil

Objective: To isolate Candida albicans(C. albicans) from the urine of hospitalized patients and assess the virulence factors and antifungal susceptibility profiles of the ***: Yeasts were identified using the chromogenic medium CHROMagar?, the VITEK~?2 system, hypertonic Sabouraud broth, tobacco agar, polymerase chain reaction,and DNA sequencing. The evaluated virulence factors were proteinase production,phospholipase production, and biofilm production on polystyrene. The broth microdilution technique was used to determine the minimum inhibitory ***: All yeasts isolated from urine were identified as C. albicans using both classical and molecular methods. Although 91.3% of the isolates showed no phospholipase activity, 56.5% showed strong proteinase activity and 91.7% produced biofilm. All microorganisms were sensitive to fluconazole, voriconazole and amphotericin B, but 56.5% of the yeasts showed resistance to ***: C. albicans isolates from urine have a high capacity for virulence and can be associated with infectious processes. Furthermore, the high percentage of isolates resistant to itraconazole is important because this antifungal agent is commonly used to treat fungal infections in the hospital environment.

关键词： Candidiasis Urinary tract Infection Virulence Antifungal susceptibility

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WJSC 6^(th) Anniversary Special Issues(2):Mesenchymal stem cells Neurotrauma and mesenchymal stem cells treatment:From experimental studies to clinical trials

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World Journal of Stem Cells 2014年第2期6卷 179-194页

作者： Ana Maria Blanco Martinez Camila de oliveira Goulart bruna dos Santos Ramalho Júlia Teixeira oliveira Fernanda Martins Almeida Laboratory of Neurodegeneration and Repair Institute of Biomedical SciencesHealth Science Center Pathology department Faculty of MedicineFederal University of Rio de Janeiro Federal University of Rio de Janeiro Campus Macaé

Mesenchymal stem cell(MSC)therapy has attracted the attention of scientists and clinicians around the *** and pre-clinical experimental studies have highlighted the positive effects of MSC treatment after spinal cord and peripheral nerve *** effects are believed to be due to their ability to differentiate into other cell lineages,modulate inflammatory and immunomodulatory responses,reduce cell apoptosis,secrete several neurotrophic factors and respond to tissue injury,among *** are many pre-clinical studies on MSC treatment for spinal cord injury(SCI)and peripheral nerve ***,the same is not true for clinical trials,particularly those concerned with nerve trauma,indicating the necessity of more well-constructed studies showing the benefits that cell therapy can provide for individuals suffering the consequences of nerve *** for clinical trials for SCI treatment the results obtained so far are not as beneficial as those described in experimental *** these reasons basic and pre-clinical studies dealing with MSC therapy should emphasize the standardization of protocols that could be translated to the clinical set with consistent and positive *** review is based on pre-clinical studies and clinical trials available in the literature from 2010 until *** the time of writing this article there were 43 and 36 pre-clinical and 19 and 1 clinical trials on injured spinal cord and peripheral nerves,respectively.

关键词： Neurotrauma Stem cell therapy Mesenchymal stem cells Pre-clinical studies Clinical trials

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Hepatobiliary phases in magnetic resonance imaging using liverspecific contrast for focal lesions in clinical practice

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World Journal of Hepatology 2022年第7期14卷 1459-1469页

作者： Daniel Alvarenga Fernandes Eduardo Andreazza Dal Lago Felipe Aguera Oliver bruna Melo Coelho Loureiro Daniel Lahan Martins Thiago José Penachim Ricardo Hoelz de oliveira Barros José de ArimatéiaBatista Araújo Filho Larissa Bastos Eloy da Costa Áurea Maria oliveira da Silva Elaine Cristina de Ataíde Ilka de Fátima Santana Ferreira Boin Nelson Marcio Gomes Caserta department of Radiology School of Medical SciencesUniversity of Campinas-UNICAMPCampinas 13083-888São PauloBrazi department of Radiology Medical SchoolSão Paulo State University-UNESPBotucatu 18618-970São PauloBrazil Instituto de Radiologia Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo-InRad/HC-FMUSPSão Paulo 05403-010SPBrazil department of Radiology Sírio-Libanês HospitalSão Paulo 05652-900SPBrazil department of Pathology School of Medical SciencesUniversity of Campinas-UNICAMPCampinas 13083-888São PauloBrazil Liver Transplant Unit Department of SurgerySchool of Medical SciencesUniversity of Campinas-UNICAMPCampinas 13083-888São PauloBrazil

BACKGROUND Challenging lesions,difficult to diagnose through non-invasive methods,constitute an important emotional burden for each patient regarding a still uncertain diagnosis(malignant x benign).In addition,from a therapeutic and prognostic point of view,delay in a definitive diagnosis can lead to worse *** of the main innovative trends currently is the use of molecular and functional methods to *** liver-specific contrast agents havebeen developed and studied in recent years to improve the performance of liver magnetic resonance imaging(MRI).More recently,one of the contrast agents introduced in clinical practice is gadoxetic acid(gadoxetate disodium).AIM To demonstrate the value of the hepatobiliary phases using gadoxetic acid in MRI for the characterization of focal liver lesions(FLL)in clinical *** Overall,302 Lesions were studied in 136 patients who underwent MRI exams using gadoxetic acid for the assessment of *** radiologists independently reviewed the MRI exams using four stages,and categorized them on a 6-point scale,from 0(lesion not detected)to 5(definitely malignant).The stages were:stage 1-images without contrast,stage 2-addition of dynamic phases after contrast(analogous to usual extracellular contrasts),stage 3-addition of hepatobiliary phase after 10 min(HBP 10’),stage 4-hepatobiliary phase after 20 min(HBP 20’)in addition to stage *** The interobserver agreement was high(weighted Kappa coefficient:0.81-1)at all stages in the characterization of benign and malignant *** diagnostic weighted accuracy(Az)was 0.80 in stage 1 and was increased to 0.90 in stage *** of the hepatobiliary phase increased Az to 0.98 in stage 3,which was also 0.98 in stage *** The hepatobiliary sequences improve diagnostic *** growing potential in the era of precision medicine,the improvement and dissemination of the method among medical specialties can bring benefits in the management of patients w

关键词： Liver Liver neoplasms Liver transplantation Medical oncology Diagnostic imaging Magnetic resonance imaging

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Mouthwash with Active Oxygen (blue<sup>®</sup>m) Induces Keratinocytes Proliferation

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Open Journal of Stomatology 2020年第6期10卷 107-114页

作者： bruna Marca Mattei Soraia A. W. Imanishi Grasieli de oliveira Ramos Paloma Santos de Campos Suyany Gabriely Weiss Tatiana Miranda deliberador department of dentistry Universidade do Oeste de Santa Catarina (UNOESC) Joa&#231 aba Brazil department of dentistry Universidade Federal do Rio Grande do Sul (UFRGS) Porto Alegre Brazil School of Health Sciences Department of Dentistry Universidade Positivo Curitiba Brazil

Chlorhexidine is widely used in dentistry to treat various gingival conditions. Its side effects are widely described. Histologically, the gingiva consists of a stratified squamous epithelium, with a predominance of keratinocytes, the latter being fundamental in healing processes. Thus, the objective of this in vitro study was to evaluate the effects of a new product with active oxygen (blue®m) on keratinocytes. Keratinocytes (HACAT) were incubated with different concentrations of blue®m (1, 10 and 100 μl/ml), and another well was used as a control, without the presence of mouthwash. After 24, 48 and 72 hours, cell proliferation was analyzed by CyQUANT®. It was possible to observe that lower concentrations (1 μl/ml) of blue

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Functional biomarker signatures of circulating T-cells and its association with distinct clinical status of leprosy patients and their respective household contacts

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Infectious Diseases of Poverty 2020年第6期9卷 5-15页

作者： Pedro Henrique Ferreira Marçal Rafael Silva Gama Lorena bruna Pereira de oliveira Olindo Assis Martins-Filho Roberta Olmo Pinheiro Euzenir Nunes Sarno Milton Ozório Moraes Lucia Alves de oliveira Fraga Universidade Vale do Rio Doce–Univale Governador ValadaresMGBrazil Grupo Integrado de Pesquisas em Biomarcadores Institute Rene RachouFIOCRUZ-MinasBelo HorizonteMGBrazil Laboratorio de Hanseniase Instituto Oswaldo CruzFundagao Oswaldo Cruz-FIOCRUZ-RJRio de JaneiroRJBrazil Nucleo de Pesquisa em Hansenologia Universidade Federal de Juiz de ForaInstituto de Ciencias da VidaCam pus Governador ValadaresRua Sao Paulo582-CentroGovernador ValadaresMG 30190-002Brazil

Background: Leprosy is a chronic infectious disease classified into two subgroups for therapeutic purposes:paucibacillary(PB)and multibacillary(MB),closely related to the host immune *** this context it is noteworthy looking for immunological biomarkers applicable as complementary diagnostic tools as well as a laboratorial strategy to follow-up leprosy household ***:: The cross-sectional study enrolled 49 participants,including 19 patients and 30 healthy *** blood mononuclear cells(PBMC)were isolated and incubated in the presence of Mycobacterium leprae *** cells were prepared for surface(CD4+and CD8+)and intracytoplasmic cytokine staining(IFN-γ,IL-4 and IL-10).Multiple comparisons amongst groups were carried out by ANOVA,Kruskal–Wallis,Student T or Mann–Whitney *** analysis of categorical variables was performed by *** biomarker signature analysis was conducted using the global median values for each biomarker index as the cut-off edge to identify the proportion of subjects with high biomarker ***: The cytokine signature analysis demonstrated that leprosy patients presented a polyfunctional profile of T-cells subsets,with increased frequency of IFN-γ+T-cell subsets along with IL-10+and IL-4+from CD4+T-cells,as compared to health Controls(Venn diagram report).Moreover,statistical analysis was carried out using parametric or non-parametric variance analysis followed by pairwise multiple comparisons,according to the data normality distribution.L(PB)displayed a polyfunctional profile characterized by enhanced percentage of IFN-γ+,IL-10+and IL-4+produced by most T-cell subsets,as compared to L(MB)that presented a more restricted cytokine functional profile mediated by IL-10+and IL-4+T-cells with minor contribution of IFN-γproduced by CD4+*** was that HHC(MB)exhibited enhanced frequency of IFN-γ+T-cells,contrasting with HHC(PB)that presented a cytokine profile limited t

关键词： Leprosy Mycobacterium leprae Cytokines Household contacts

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