Functional biomarker signatures of circulating T-cells and its association with distinct clinical status of leprosy patients and their respective household contacts

作     者：Pedro Henrique Ferreira Marçal Rafael Silva Gama Lorena Bruna Pereira de Oliveira Olindo Assis Martins-Filho Roberta Olmo Pinheiro Euzenir Nunes Sarno Milton Ozório Moraes Lucia Alves de Oliveira Fraga Pedro Henrique Ferreira Marçal;Rafael Silva Gama;Lorena Bruna Pereira de Oliveira;Olindo Assis Martins-Filho;Roberta Olmo Pinheiro;Euzenir Nunes Sarno;Milton Ozório Moraes;Lucia Alves de Oliveira Fraga

作者机构：Universidade Vale do Rio Doce–UnivaleGovernador ValadaresMGBrazil Grupo Integrado de Pesquisas em BiomarcadoresInstitute Rene RachouFIOCRUZ-MinasBelo HorizonteMGBrazil Laboratorio de HanseniaseInstituto Oswaldo CruzFundagao Oswaldo Cruz-FIOCRUZ-RJRio de JaneiroRJBrazil Nucleo de Pesquisa em HansenologiaUniversidade Federal de Juiz de ForaInstituto de Ciencias da VidaCam pus Governador ValadaresRua Sao Paulo582-CentroGovernador ValadaresMG 30190-002Brazil 

出 版 物：《Infectious Diseases of Poverty》 (贫困所致传染病（英文）)

年 卷 期：2020年第9卷第6期

页      面：5-15页

核心收录：

学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100206[医学-皮肤病与性病学] 10[医学] 

基　　金：This study received financial support from the Conselho de Desenvolvimento Tecnológico e Científico/CNPq/BRAZIL DECIT 2008 DECIT 2012 FAPEMIG TC 304/2013/FNS/MS and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior(CAPES).OAMF received PQ fellowships from CNPq.These fundings sources had no role in the design of the study and collection analysis implementation and interpretation of data and in writing the manuscript 

主　　题：Leprosy Mycobacterium leprae Cytokines Household contacts 

摘      要：Background: Leprosy is a chronic infectious disease classified into two subgroups for therapeutic purposes:paucibacillary(PB)and multibacillary(MB),closely related to the host immune *** this context it is noteworthy looking for immunological biomarkers applicable as complementary diagnostic tools as well as a laboratorial strategy to follow-up leprosy household ***:: The cross-sectional study enrolled 49 participants,including 19 patients and 30 healthy *** blood mononuclear cells(PBMC)were isolated and incubated in the presence of Mycobacterium leprae *** cells were prepared for surface(CD4+and CD8+)and intracytoplasmic cytokine staining(IFN-γ,IL-4 and IL-10).Multiple comparisons amongst groups were carried out by ANOVA,Kruskal–Wallis,Student T or Mann–Whitney *** analysis of categorical variables was performed by *** biomarker signature analysis was conducted using the global median values for each biomarker index as the cut-off edge to identify the proportion of subjects with high biomarker ***: The cytokine signature analysis demonstrated that leprosy patients presented a polyfunctional profile of T-cells subsets,with increased frequency of IFN-γ+T-cell subsets along with IL-10+and IL-4+from CD4+T-cells,as compared to health Controls(Venn diagram report).Moreover,statistical analysis was carried out using parametric or non-parametric variance analysis followed by pairwise multiple comparisons,according to the data normality distribution.L(PB)displayed a polyfunctional profile characterized by enhanced percentage of IFN-γ+,IL-10+and IL-4+produced by most T-cell subsets,as compared to L(MB)that presented a more restricted cytokine functional profile mediated by IL-10+and IL-4+T-cells with minor contribution of IFN-γproduced by CD4+*** was that HHC(MB)exhibited enhanced frequency of IFN-γ+T-cells,contrasting with HHC(PB)that presented a cytokine profile limited t