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电子设计技术 1994年第10期 39-40+43-45页

作者： bruce d.Moore

使用四节硷性AA电池构成稳压器作为袖珍电子设备的电源,不仅应该能调节,而且也具备效率高、补偿电流和体积小等功能, 从而引出了各种稳压器结构的设计,供设计人员选择。

关键词：稳压器四节电源输入电压电池组峰值电流

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对硫磷纳米抗体筛选及分子识别机制研究

分析化学 2019年第9期47卷 1419-1428页

作者：张玉琪张瑾如王锋李家冬司睿吕丽珊沈玉栋王弘 HAMMOCK bruce d 孙远明华南农业大学食品学院广东省食品质量与安全重点实验室畜禽产品精准加工与安全控制技术国家地方联合工程研究中心(广东) 广州安诺科技股份有限公司 department of Entomology and UCd Comprehensive Cancer Center University of CaliforniaDavis

对硫磷是一种广谱的有机磷酸酯类杀虫、杀螨剂,毒性极强,可对环境和人类健康造成严重危害。尽管我国已禁止使用对硫磷,但仍存在违禁使用现象,加强对其监测十分必要。纳米抗体是已知最小的与抗原结合的片段（15 kda）,来源于骆驼科动物... 详细信息

对硫磷是一种广谱的有机磷酸酯类杀虫、杀螨剂,毒性极强,可对环境和人类健康造成严重危害。尽管我国已禁止使用对硫磷,但仍存在违禁使用现象,加强对其监测十分必要。纳米抗体是已知最小的与抗原结合的片段（15 kda）,来源于骆驼科动物体内重链抗体的可变区,具有稳定性强、灵敏度高、易表达、水溶性好等特性。本研究基于噬菌体展示技术构建了抗对硫磷纳米抗体库,其库容为2.41×10;cfu/mL,经抗原-抗体固相亲和筛选及噬菌体酶联免疫法（Phage ELISA）鉴定,获得5种抗对硫磷纳米抗体VHH1、VHH6、VHH13、VHH21和VHH24。以结合能力最强的VHH1为基础,采用同源建模和分子对接技术探讨VHH1与对硫磷分子的识别机制,发现VHH1与对硫磷存在氢键和疏水间分子作用力,关键氨基酸是Ser60、Lys104、Phe105、Gly106和Arg107。本研究为抗体的进化改造和半抗原的设计提供了新思路。

关键词：纳米抗体对硫磷噬菌体展示分子模拟

A highly sensitive bio-barcode immunoassay for multi-residue detection of organophosphate pesticides based on fluorescence antiquenching

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Journal of Pharmaceutical Analysis 2022年第4期12卷 637-644页

作者： Lingyuan Xu Xiuyuan Zhang A.M.Abd El-Aty Yuanshang Wang Zhen Cao Huiyan Jia J.-Pablo Salvador Ahmet Hacimuftuoglu Xueyan Cui Yudan Zhang Kun Wang Yongxin She Fen Jin Lufei Zheng Baima Pujia Jing Wang Maojun Jin bruce d.Hammock Institute of Quality Standard and Testing Technology for Agro-Products Chinese Academy of Agricultural SciencesBeijing100081China department of Pharmacology Faculty of Veterinary MedicineCairo UniversityGiza12211Egypt department of Medical Pharmacology Medical FacultyAtaturk UniversityErzurum25240Türkiye Institute of Livestock and Poultry Ningbo Academy of Agricultural SciencesNingboZhejiang315040China Nanobiotechnology for diagnostics Group Instituto de Química Avanzada de CatalunaIQAC-CSICBarcelona08034Spain CIBER de Bioingeniería Biomateriales y Nanomedicina(CIBER-BBN)Madrid28029Spain Inspection and Testing Center of Agricultural and Livestock Products of Tibet Department of Agriculture and Rural Affairs of Tibet Autonomous RegionLhasa850000China department of Entomology&Nematology and the UC davis Comprehensive Cancer Center University of CaliforniaDavisCA95616USA

Balancing the risks and benefits of organophosphate pesticides(OPs)on human and environmental health relies partly on their accurate measurement.A highly sensitive fluorescence anti-quenching multi-residue bio-barcode immunoassay was developed to detect OPs(triazophos,parathion,and chlorpyrifos)in apples,turnips,cabbages,and *** nanoparticles were functionalized with monoclonal antibodies against the tested *** oligonucleotides were complementarily hybridized with an RNA fluorescent label for signal *** detection signals were generated by dNA-RNA hybridization and ribonuclease H dissociation of the *** resulting fluorescence signal enables multiplexed quantification of triazophos,parathion,and chlorpyrifos residues over the concentration range of 0.01-25,0.01-50,and 0.1-50 ng/mL with limits of detection of 0.014,0.011,and 0.126 ng/mL,*** mean recovery ranged between 80.3% and 110.8% with relative standard deviations of 7.3%-17.6%,which correlate well with results obtained by liquid chromatography-tandem mass spectrometry(LC-MS/MS).The proposed bio-barcode immunoassay is stable,reproducible and reliable,and is able to detect low residual levels of multi-residue OPs in agricultural products.

关键词： Organophosphate pesticides Fluorescence anti-quenching Gold nanoparticles dNA-RNA hybridization dNA oligonucleotides Ribonuclease H

Resilience in a Mexican Pacific Mangrove after Hurricanes: Implications for Conservation Restoration

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Journal of Environmental Protection 2013年第12期4卷 1383-1391页

作者： Mateo d. M. Ruiz bruce Taylor José Luis Rangel-Salazar Bonifacio Cortés Hernández department of Biodiversity Conservation El Colegio de la Frontera Sur San Cristóbal de Las Casas Campus Chiapas Mexico Reforestation Nursery Servicios Ecoturísticos La Ventanilla Cooperative Society Puchutla Mexico

High resilience is a mangrove trait that is compatible with life in a dynamic environment, however, catastrophic disturbance can lead to the entire forest structure being re-defined. In the Pochutla district of Oaxaca, two hurricanes made landfall in 1997 and one more in 2012. Following the 1997 hurricanes, extensive mangrove restoration was carried out in the study area. A cohort of Rhizophora mangle saplings planted in 2007 showed 10% mortality during the first year after transplantation, an average growth of 39 cm, and a positive association between growth rate and the level of water in the lagoon. Following Hurricane Carlotta of 2012, measurements of structural impact and tree mortality were taken in restored R. mangle stands and in naturally regenerated patches of Laguncularia racemosa. The role of tree girth in R. mangle susceptibility to wind damage was also investigated. The stands of R. mangle suffered 80% reduction in stem density and 86% loss of basal area, whereas the corresponding values for L. racemosa were 26% and 15%, respectively. Within stands of R. mangle, mortality conserved a positive relationship with structural impact categories and the frequency of snapped stems was segregated across girth classes. The results suggest that L. racemosa has greater resilience to hurricane damage, which has some consistency with previous research and implications for conservation restoration protocols. In order to promote a system with higher resilience, we recommend an on-going restoration effort with mixed mangrove species.

关键词： Coastal Lagoon disturbance Hurricane Mangrove Restoration Resilience

Examining the use of narrative constructs in data videos

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Visual Informatics 2020年第1期4卷 8-22页

作者： Ruochen Cao Subrata dey Andrew Cunningham James Walsh Ross T.Smith Joanne E.Zucco bruce H.Thomas The School of Information Technology and Mathematical Science University of South AustraliaAustralia department of Media University of AdelaideAustralia

data videos are a highly impactful method of communication and are becoming a prevalent medium for communicating *** the majority of current research focuses on the cinematic aspects of data videos,very little is known about the narrative methodologies *** paper presents our insights derived from an initial exploration of this *** present a taxonomy based on the analysis of 70 existing data videos examining their narrative and visual *** propose that our taxonomy can be used to explain the characteristics or design of data *** this taxonomy,we present our observations,including the trend of popular technologies applied in current data videos,the under-utilization of promising methods,and highlight research opportunities in the field.

关键词： Narrative visualization data videos Taxonomy Narrative approaches

Characterization of spermidine hydroxycinnamoyl transferases from eggplant (Solanum melongena L.) and its wild relative Solanum richardii dunal

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Horticulture Research 2016年第1期3卷 21-29页

作者： Hui Peng Tianbao Yang bruce d Whitaker Frances Trouth Lingfei Shangguan Wen dong Wayne M Jurick Agricultural Research Service of U.S.department of Agriculture Food Quality LaboratoryBeltsville Agricultural Research CenterBeltsville20705MDUSA Horticulture&Landscape College Hunan Agricultural UniversityChangsha410128HunanChina College of Horticulture Nanjing Agricultural UniversityNanjing210095JiangsuChina department of Plant Science University of TennesseeKnoxville37996TNUSA

Eggplant produces a variety of hydroxycinnamic acid amides(HCAAs)that have an important role in plant development and adaptation to environmental *** this study,we identified and characterized a spermidine hydroxycinnamoyl transferase(SHT)from eggplant(Solanum melongena)and its wild relative ***,designated as SmSHT and SrSHT,*** was abundant in flowers and fruits,whereas the level of SrSHT was remarkably low in all ***-shock/drought treatment stimulated the expression of SmSHT in both leaves and fruits,indicating its involvement in plant stress *** SHT polypeptides had extremely high identity with just five amino-acid *** SmSHT catalyzed the synthesis of mono-,bi-and tri-acylated *** caffeoyl-CoA as the acyl donor,SmSHT preferred spermidine as the acyl *** spermidine was the acyl acceptor,the donor preference order for SmSHT was caffeoyl-CoA>feruloyl-CoA>ρ-*** exhibited the same substrate specificity as SmSHT,yet exhibited significantly higher catalytic activity than *** example,under caffeoyl-CoA and spermidine,Kcat of SrSHT was 37.3%higher than *** modeling suggests that five amino-acid substitutions in SrSHT result in four alterations in their predicted 3d *** particular,the conserved Lys402 adjacent to the dFGWG motif,and Cys200 in the crossover loop in SmSHT were replaced by Glu and Ser in *** substitutions may contribute to the enhanced activity in *** study provides a platform to generate HCAA rich fruits for eggplant and other solanaceous crops.

关键词： donor acceptor flowers

电子战在“沙漠风暴”中起的作用比以往任何冲突中起的作用都大

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电子对抗技术 1991年第4期6卷 39-42页

作者： bruce d Nordwall 顾耀平

据返航的战斗飞行员以及五角大楼的官员们说,电子战在“沙漠风暴”中起的作用比以往任何一次冲突中起的作用都大。一位海军军部官员说,干扰机提供的保护极其重要,以致于如果伴随飞行的EA-6B电子战飞机被击落,则海军/海军陆战队的任务就... 详细信息

据返航的战斗飞行员以及五角大楼的官员们说,电子战在“沙漠风暴”中起的作用比以往任何一次冲突中起的作用都大。一位海军军部官员说,干扰机提供的保护极其重要,以致于如果伴随飞行的EA-6B电子战飞机被击落,则海军/海军陆战队的任务就无法执行。正常工作的雷达告警接收机(RWR)和自卫设备也是每架战术飞机的必备先决条件。飞行员关于电子战系统(作用)价值的评论正在开始透露出来。一位空军飞行员告诉西屋公司在战场上的工程师说:“谢谢,你们的ALQ-131吊舱保护了我的机尾。他们(伊军)向我发射一枚“萨姆”弹(地空导弹),吊舱就发射干扰,‘萨姆’就成了‘傻瓜’朝着错误的方向飞去。”另一个飞行员说:“如果不是因为有ECM吊舱或机尾告警,那我们的飞机有50％是回不来的。”

关键词：电子对抗海弯战争沙漠风暴

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Involutions Fixing the disjoint Union of Copies of Even Projective Space

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Acta Mathematica Sinica,English Series 1996年第2期12卷 162-166页

作者： Hou duo bruce Torrence Hou duo department of Mathematics Hebei Teachers University Shijiazhuang,050016 Chinabruce Torrence department of Mathematics Georgetown University Washington,d.C.20057 U.S.A.

We show that for any differentiable involution on an r-dimensional manifold(M,T) whose fixed point set F is a disjoint union of real projective spaces of constant dimension 2n,we have:if r=4n then(M,T)is bordant to(F... 详细信息

关键词： Involution Cobordism

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The electronic evidence and discovery handbook

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1000年

作者： Sharon d. Nelson bruce A. Olson John W. Simek

来源：畅想之星图书光盘版（南... 评论

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畅想之星图书光盘版（南通市图书馆镜像）

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高等教育财政：问题与出路第1版