Characterization of spermidine hydroxycinnamoyl transferases from eggplant (Solanum melongena L.) and its wild relative Solanum richardii Dunal
作者机构:Agricultural Research Service of U.S.Department of AgricultureFood Quality LaboratoryBeltsville Agricultural Research CenterBeltsville20705MDUSA Horticulture&Landscape CollegeHunan Agricultural UniversityChangsha410128HunanChina College of HorticultureNanjing Agricultural UniversityNanjing210095JiangsuChina Department of Plant ScienceUniversity of TennesseeKnoxville37996TNUSA
出 版 物:《Horticulture Research》 (园艺研究(英文))
年 卷 期:2016年第3卷第1期
页 面:21-29页
核心收录:
学科分类:081704[工学-应用化学] 07[理学] 08[工学] 070303[理学-有机化学] 0710[理学-生物学] 0831[工学-生物医学工程(可授工学、理学、医学学位)] 1001[医学-基础医学(可授医学、理学学位)] 0817[工学-化学工程与技术] 0901[农学-作物学] 0703[理学-化学] 0836[工学-生物工程] 0902[农学-园艺学] 0834[工学-风景园林学(可授工学、农学学位)]
基 金:This research was funded by USDA-ARS NP306 project no.8042-43000-012-00D
摘 要:Eggplant produces a variety of hydroxycinnamic acid amides(HCAAs)that have an important role in plant development and adaptation to environmental *** this study,we identified and characterized a spermidine hydroxycinnamoyl transferase(SHT)from eggplant(Solanum melongena)and its wild relative ***,designated as SmSHT and SrSHT,*** was abundant in flowers and fruits,whereas the level of SrSHT was remarkably low in all ***-shock/drought treatment stimulated the expression of SmSHT in both leaves and fruits,indicating its involvement in plant stress *** SHT polypeptides had extremely high identity with just five amino-acid *** SmSHT catalyzed the synthesis of mono-,bi-and tri-acylated *** caffeoyl-CoA as the acyl donor,SmSHT preferred spermidine as the acyl *** spermidine was the acyl acceptor,the donor preference order for SmSHT was caffeoyl-CoAferuloyl-CoAρ-*** exhibited the same substrate specificity as SmSHT,yet exhibited significantly higher catalytic activity than *** example,under caffeoyl-CoA and spermidine,Kcat of SrSHT was 37.3%higher than *** modeling suggests that five amino-acid substitutions in SrSHT result in four alterations in their predicted 3D *** particular,the conserved Lys402 adjacent to the DFGWG motif,and Cys200 in the crossover loop in SmSHT were replaced by Glu and Ser in *** substitutions may contribute to the enhanced activity in *** study provides a platform to generate HCAA rich fruits for eggplant and other solanaceous crops.