Characterization of spermidine hydroxycinnamoyl transferases from eggplant (Solanum melongena L.) and its wild relative Solanum richardii Dunal

作     者：Hui Peng Tianbao Yang Bruce D Whitaker Frances Trouth Lingfei Shangguan Wen Dong Wayne M Jurick 

作者机构：Agricultural Research Service of U.S.Department of AgricultureFood Quality LaboratoryBeltsville Agricultural Research CenterBeltsville20705MDUSA Horticulture&Landscape CollegeHunan Agricultural UniversityChangsha410128HunanChina College of HorticultureNanjing Agricultural UniversityNanjing210095JiangsuChina Department of Plant ScienceUniversity of TennesseeKnoxville37996TNUSA 

出 版 物：《Horticulture Research》 (园艺研究（英文）)

年 卷 期：2016年第3卷第1期

页      面：21-29页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 070303[理学-有机化学] 0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1001[医学-基础医学(可授医学、理学学位)] 0817[工学-化学工程与技术] 0901[农学-作物学] 0703[理学-化学] 0836[工学-生物工程] 0902[农学-园艺学] 0834[工学-风景园林学（可授工学、农学学位）] 

基　　金：This research was funded by USDA-ARS NP306 project no.8042-43000-012-00D 

主　　题：donor acceptor flowers 

摘      要：Eggplant produces a variety of hydroxycinnamic acid amides(HCAAs)that have an important role in plant development and adaptation to environmental *** this study,we identified and characterized a spermidine hydroxycinnamoyl transferase(SHT)from eggplant(Solanum melongena)and its wild relative ***,designated as SmSHT and SrSHT,*** was abundant in flowers and fruits,whereas the level of SrSHT was remarkably low in all ***-shock/drought treatment stimulated the expression of SmSHT in both leaves and fruits,indicating its involvement in plant stress *** SHT polypeptides had extremely high identity with just five amino-acid *** SmSHT catalyzed the synthesis of mono-,bi-and tri-acylated *** caffeoyl-CoA as the acyl donor,SmSHT preferred spermidine as the acyl *** spermidine was the acyl acceptor,the donor preference order for SmSHT was caffeoyl-CoAferuloyl-CoAρ-*** exhibited the same substrate specificity as SmSHT,yet exhibited significantly higher catalytic activity than *** example,under caffeoyl-CoA and spermidine,Kcat of SrSHT was 37.3%higher than *** modeling suggests that five amino-acid substitutions in SrSHT result in four alterations in their predicted 3D *** particular,the conserved Lys402 adjacent to the DFGWG motif,and Cys200 in the crossover loop in SmSHT were replaced by Glu and Ser in *** substitutions may contribute to the enhanced activity in *** study provides a platform to generate HCAA rich fruits for eggplant and other solanaceous crops.