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Role of CYP311A1 in wing development of Drosophila melanogaster

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Insect Science 2024年第3期31卷 748-758页

作者： Xubo Zhang Mengqi Liu Andi Cheng bernard moussian Jianzhen Zhang Wei Dong Shanxi Key Laboratory of Nucleic Acid Biopesticides Institute of Applied BiologyShanxi UniversityTaiyuanChina College of Life Science Shanxi UniversityTaiyuanChina INRAE CNRSInstitut Sophia AgrobiotechSophia AntipolisUniversite Cote d'AzurNiceFrance

Lipid homeostasis is crucial for growth and development of *** cytochrome P450 monooxygenases(CYPs)are involved in lipid *** function of Cyp3llal in the anterior midgut as a regulator of phosphatidylethanolamine(PE)metabolism in Drosophila melanogaster has been demonstrated,as depletion of Cyp31lal caused larval growth arrest that was partially rescued by supplying *** this study,we investigated the role of CYP311A1 in wing morphogenesis in *** the GAL4-UAS system,Cyp31lal was selectively knocked down in the wing disc.A deformed wing phenotype was observed in flies with reduced Cyp31lal *** and oil red O staining revealed a reduction of neutral lipids in the wing disc after the depletion of *** addition,we observed an enhanced sensitivity to Eosin Y penetration in the wings of Cyp31lal knocked-down ***,the reduction of CYP311A1 function in developing wings does not affect cell proliferation and apoptosis,but entails disordered Phalloidin or Cadherin distribution,suggesting an abnormal cell morphology and cell cortex structure in wing epithelial *** together,our results suggest that Cyp3llal is needed for wing morphogenesis by participating in lipid assembly and cell homeostasis.

关键词： apoptosis cell cortex cell proliferation Cyp31lal lipid wing

The apical plasma membrane of chitin-synthesizing epithelia

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Insect Science 2013年第2期20卷 139-146页

作者： bernard moussian Animal Genetics Interfaculty Institute for Cell Biology University of Tubingen 72076 TEtbingen Germany

Chitin is the second most abundant polysaccharide on earth. It is produced at the apical side of epidermal, tracheal, fore-, and hindgut epithelial cells in insects as a central component of the protective and supporting extracellular cuticle. Chitin is also an important constituent of the midgut peritrophic matrix that encases the food supporting its digestion and protects the epithelium against invasion by possibly ingested pathogens. The enzyme producing chitin is a glycosyltransferase that resides in the apical plasma membrane forming a pore to extrude the chains of chitin into the extracellular space. The apical plasma membrane is not only a platform for chitin synthases but, probably through its shape and equipment with distinct factors, also plays an important role in orienting and organizing chitin fibers. Here, I review findings on the cellular and molecular constitution of the apical plasma membrane of chitin-producing epithelia mainly focusing on work done in the fruit fly Drosophila melanogaster.

关键词： apical undulae cuticle extracellular matrix epidermis microvilli

Chitinase 10 controls chitin amounts and organization in the wing cuticle of Drosophila

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Insect Science 2020年第6期27卷 1198-1207页

作者： Wei Dong Ying-Hao Gao Xu-Bo Zhang bernard moussian Jian-Zhen Zhang Institute of Applied Biology Shanxi UniversityTaiyuanChina College of Life Science Shanxi UniversityTaiyuanChina Interfaculty Institute of Cell Biology University of TubingenTubingenGermany Universite Cote d'Azur CNRSInsermiBVParc ValroseNice CEDEX 2France

Wings are essential for insect fitness.A number of proteins and enzymes have been identified to be involved in wing terminal differentiation,which is characterized by the formation of the wing ***,we addressed the question whether chitinase 10(Cht10)may play an important role in chitin organization in the wings of the fruit fly Drosophila ***,we first found that Cht10 expression coincides with the expression of the chitin synthase coding gene *** suggests that the respective proteins may cooperate during wing *** tissue-specific RNA interference experiments,we demonstrate that suppression of Cht10 causes an excess in chitin amounts in the wing *** organization is severely disrupted in these *** on these data,we hypothesize that Cht10 restricts chitin amounts produced by Kkv in order to ensure normal chitin organization and wing cuticle *** addition,we found by scanning electron microscopy that Cht10 suppression also affects the cuticle *** turn,cuticle inward permeability is enhanced in Cht10 Aess ***,flies with reduced Cht 10 function are unable to *** conclusion,Cht10 is essential for wing terminal differentiation and function.

关键词： Cht10 chitin cuticle Drosophila melanogaster,Kkv wing

BIOINSECTICIDES AS FUTURE MAINSTREAM PEST CONTROL AGENTS: OPPORTUNITIES AND CHALLENGES

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Frontiers of Agricultural Science and Engineering 2022年第1期9卷 82-97页

作者： Mingbo QU Hans MERZENDORFER bernard moussian Qing YANG School of Bioengineering Dalian University of TechnologyDalian 116024China State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China Institute of Biology University of Siegen57076 SiegenGermany Interfaculty Institute of Cell Biology University of Tübingen72076 TübingenGermany Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120China

Bioinsecticides are naturally-occurring substances from different sources that control insect *** bioinsecticides should have low toxicity to non-target *** should also be easily degraded in sewage treatment works and natural environments,highly effective in small quantities and affect target pests *** concerns about possible side-effects of synthetic pesticides have accelerated bioinsecticide research and ***,to develop bioinsecticides into mainstream products,their high production costs,short shelf-life and often uncertain modes of action need to be *** review summarizes current progress on bioinsecticides which are categorized as biochemical insecticides and their derivatives,plant-incorporated protectants,and microbial *** current constraints that prevent bioinsecticides from being widely used are discussed and future research directions are proposed.

关键词： biochemical insecticide bioinsecticide microbial bioinsecticides plant-incorporated protectant RNA insecticide

Flexible manipulation of Omb levels in the endogenous expression region of Drosophila wing by combinational overexpression and suppression strategy

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Insect Science 2020年第1期27卷 14-21页

作者： Xu-Bo Zhang Wei Dong Kai-Xia Li Juan-Juan Wang Jie Shen bernard moussian Jian-Zhen Zhang Institute of Applied Biology College of Life ScienceShanxi UniversityTaiyuanChina Department of Entomology China Agricultural UniversityBeijingChina Applied Zoology Technical University DresdenZellescher Weg 20bDresdenGermany iBV University of Nice Sophia-AntipolisParc ValroseNiceFrance

Manipulating an exogenous or endogenous gene of interest at a defined level is critical for a wide variety of *** Gal4/UAS system has been widely used to direct gene expression for studying complex genetic and biological problems in Drosophila melanogaster and other model *** by a given tissue-specific Gal4,expressing UAS-transgene or UAS-RNAi(RNA interference)could be used to up-or down-regulate target gene expression,***,the efficiency of the Gal4/UAS system is roughly predefined by properties of transposon vector constructs and the insertion site in the transgenic ***,we describe a simple way to modulate optomotor blind(omb)expression levels in its endogenous expression region of the wing *** co-expressed UAS-omb and UAS-omb-RNAi together under the control of dpp-Gal4 driver which is expressed in the omb expression region of the wing *** repression effect is more sensitive to temperature than that of *** low temperature,overexpression plays a dominant role but the efficiency is attenuated by *** contrast,at high temperature RNAi predominates in gene expression *** this strategy,we could manipulate omb expression levels at a moderate *** allows us to manipulate omb expression levels in the same tissue between overexpression and repression at different stages by temperature control.

关键词： combinational expression Drosophila expression level Gal4/UAS system omb wing disc

Chitinase 6 is required for procuticle thickening and organ shape in Drosophila wing

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Insect Science 2023年第2期30卷 268-278页

作者： Wei Dong Justin Flaven-Pouchon Ying-Hao Gao Chen-Yang Song Abeer El Wakil Jian-Zhen Zhang bernard moussian Institute of Applied Biology Shanxi UniversityTaiyuanChina Interfaculty Institute of Cell Biology University of TübingenTübingenGermany College of Life Science Shanxi UniversityTaiyuanChina Faculty of Education Department of Biological and Geological SciencesAlexandria UniversityAlexandriaEgypt INRAE CNRSUniversitéCote d’AzurInstitut Sophia AgrobiotechSophia AntipolisFrance

The polysaccharide chitin is a major scaffolding molecule in the insect *** order to be functional,both chitin amounts and chitin organization have been shown to be important *** great advances in the past decade,the molecular mechanisms of chitin synthesis and organization are not fully ***,we have characterized the function of the Chitinase 6(Cht6)in the formation of the wing,which is a simple flat cuticle organ,in the fruit fly Drosophila *** of Cht6 function by RNA interference during wing development does not affect chitin organization,but entails a thinner cuticle suggesting reduced chitin *** phenotype is opposed to the one reported recently to be caused by reduction of Cht10 *** as a consequence,cuticle permeability to xenobiotics is enhanced in Cht6-less *** also observed massive deformation of these *** addition,the shape of the abdomen is markedly changed upon abdominal suppression of ***,we found that suppression of Cht6 transcript levels influences the expression of genes coding for enzymes of the chitin biosynthesis *** finding indicates that wing epidermal cells respond to activity changes of Cht6 probably trying to adjust chitin ***,in a working model,we propose that Cht6-introduced modifications of chitin are needed for chitin synthesis to proceed *** thickness,according to our hypothesis,is in turn required for correct organ or body part *** molecular mechanisms of this processes shall be characterized in the future.

关键词： abdomen chitin Cht6 Drosophila melanogaster procuticle wing

Three-dimensional reconstruction of pore canals in the cuticle of the brown planthopper

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Science China(Life Sciences) 2021年第11期64卷 1992-1994页

作者： Dan-Ting Li Jian-Sheng Guo Xin-Qiu Wang bernard moussian Chuan-Xi Zhang Institute of Insect Science Zhejiang UniversityHangzhou 310058China State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Institute of Plant VirologyNingbo UniversityNingbo 315211China Department of Pathology of Sir Run Run Shaw Hospital Zhejiang University School of MedicineHangzhou 310058China School of Medicine Zhejiang UniversityHangzhou 310058China UniversitéCôte d’Azur CNRSInsermInstitute of Biology ValroseParc Valrose06108 Nice CEDEX 2France

Dear Editor,The insect cuticle is a multifunctional coat that defines and stabilizes the shape of the body,appendages and internal organs,serves as a barrier against water and chemical compounds,such as insecticides,prevents infection and protects against predators(moussian,2010).The surface of insects is covered by a lipid layer composed of diverse hydrocarbons,wax esters,fatty acids,fatty alcohols,sterols and *** of lipids to the body surface occurs through the cuticle by a nano-canal system consisting of pore canals(PC)and wax canals(WC).

关键词： alcohols hydrocarbons esters

Chitin synthase 1 and five cuticle protein genes are involved in serosal cuticle formation during early embryogenesis to enhance eggshells in Nilaparvata lugens

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Insect Science 2022年第2期29卷 363-378页

作者： Jia-Bao Lu Jian-Sheng Guo Xuan Chen Chen Cheng Xu-Mei Luo Xiao-Ya Zhang bernard moussian Jian-Ping Chen Jun-Min Li Chuan-Xi Zhang State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Key Laboratory of Biotechnology in Plant Protection of MOA of China and Zhejiang ProvinceInstitute of Plant VirologyNingbo UniversityNingboZhejiang ProvinceChina Institute of Insect Science Zhejiang UniversityHangzhouChina School of Medicine Zhejiang UniversityHangzhouChina UniversitéCôte d'Azur CNRSInsermInstitute of Biology ValroseNiceFrance

Many holo-and hemimetabolous insects enhance their eggshells during embryogenesis by forming a serosal cuticle(SC).To date,scholarly understanding of the SC composition and SC-related gene functions has been limited,especially for hemimetabolous *** this study,we initially performed transmission electron microscopic(TEM)observation and chitin staining of the SC in Nilaparvata lugens,a hemimetabolous rice pest known as the brown planthopper(BPH).We confirmed that the SC was a chitin-rich lamellar structure deposited gradually during the early *** RNA interference(RNAi)against Nilaparvata lugens chitin synthase 1(NlCHS1)in newly emerged and matured females resulted in decreases of egg hatchability by 100%and 76%,*** analyses revealed loss of the lamellar structure of the SC in dsNlCHS1-treated *** to temporal expression profiles,five cuticle protein coding genes,NlugCpr1/2/3/8/90,were specifically or highly expressed during the SC formation period,and NlugCpr1/2/3/90 were further detected in 72 h eggshells extract by ultra-performance liquid chromatography-tandem mass spectrometry/mass ***2/3/90 were likely three SC-specific cuticle *** observations of the SC following parental RNAi against NlugCpr1/2/3/8/90 demonstrated that NlugCpr3/8/90 were essential for SC *** study provided an understanding of the SC formation process and SC-related cuticle proteins in BPHs,which offer potential targets for pest control in the egg stage as well.

关键词： chitin synthase 1 cuticle protein eggshell embryogenesis Nilaparvata lugens serosal cuticle

Roles of LmCDA1 and LmCDA2 in cuticle formation in the foregut and hindgut of Locusta migratoria

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Insect Science 2021年第5期28卷 1314-1325页

作者： Min Zhang Peng-Juan Ma Ting-Ting Zhang Zhi-Mei Gao Pan Zhao Xiao-Jjian Liu Xue-Yao Zhang Wei-Min Liu Rong-Rong Yu bernard moussian Jian-Zhen Zhang Research Institute of Applied Biology Shanxi UniversityTaiyuanChina School of Life Science Shanxi UniversityTaiyuanChina Department of Biology Taiyuan Normal UniversityTaiyuanChina iBV Universite Cote d'AzurParc ValroseFrance

Chitin deacetylases(CDAs,including CDAI and CDA2)are considered key enzymes for body cuticle formation and tracheal morphogenesis in various insect ***,their functions in the formation of the cuticular intima of the foregut and hindgut are ***,we investigated the roles of their respective genes LmCDAI and Lm-CD42 in this process,in the hemimetabolous insect Locusta *** of LmCDAI and LmCDA2 were highly expressed both before and after molting in the *** the hindgut,their expression was high only before *** both the foregut and hindgut,LmCDA1 protein was localized in the basal half of the chitin matrix(procuti-cle),whereas LmCDA2 was detected in the upper half of the procuticle.K nockdown of LmCDAI by RNA interference(RNAi)in 5th-instar nymphs caused no visible defcts of the hindgut *** contrast,the chitinous lamellae of the cuticular intima in the foregut of knockdown animals were less compact than in control *** against LmCDA2 led to thickening of both the foregut and hindgut cuticles,with a greater number of thinner laminae than in the respective control *** together,our results show that LmCDAI and LmCDA2 have distinct,but overlapping,functions in chitin organization in the foregut ***,in the hindgut,this process seems independent of LmCDA1 activity but requires LmCDA2 ***,the CDAs reflect tissue-specific differences in cuticular organization and function,which need further detailed molecular and histological analyses for full comprehension.

关键词： CDA:cuticle digestion foregut hindgut laminae