Chitinase 10 controls chitin amounts and organization in the wing cuticle of Drosophila

作     者：Wei Dong Ying-Hao Gao Xu-Bo Zhang Bernard Moussian Jian-Zhen Zhang Wei Dong;Ying-Hao Gao;Xu-Bo Zhang;Bernard Moussian;Jian-Zhen Zhang

作者机构：Institute of Applied BiologyShanxi UniversityTaiyuanChina College of Life ScienceShanxi UniversityTaiyuanChina Interfaculty Institute of Cell BiologyUniversity of TubingenTubingenGermany Universite Cote d'AzurCNRSInsermiBVParc ValroseNice CEDEX 2France 

出 版 物：《Insect Science》 (昆虫科学（英文版）)

年 卷 期：2020年第27卷第6期

页      面：1198-1207页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 

基　　金：the National Natural Science Foundation of China(Grant No.31672364) the NSFC-DFG(Grant No.31761133021) Research Project Supported by Shanxi Scholarship Council of China(HGKY2019011) Special Talents Projects in Shanxi Province,China(201805D211019) the Program for Top Young Academic Leaders of Higher Learning Institutions of Shanxi(TYAL)2017.BM’s contribution was supported by the German Research Foundation(DFG grant MO1714/10-1) 

主　　题：Cht10 chitin cuticle Drosophila melanogaster,Kkv wing 

摘      要：Wings are essential for insect fitness.A number of proteins and enzymes have been identified to be involved in wing terminal differentiation,which is characterized by the formation of the wing ***,we addressed the question whether chitinase 10(Cht10)may play an important role in chitin organization in the wings of the fruit fly Drosophila ***,we first found that Cht10 expression coincides with the expression of the chitin synthase coding gene *** suggests that the respective proteins may cooperate during wing *** tissue-specific RNA interference experiments,we demonstrate that suppression of Cht10 causes an excess in chitin amounts in the wing *** organization is severely disrupted in these *** on these data,we hypothesize that Cht10 restricts chitin amounts produced by Kkv in order to ensure normal chitin organization and wing cuticle *** addition,we found by scanning electron microscopy that Cht10 suppression also affects the cuticle *** turn,cuticle inward permeability is enhanced in Cht10 Aess ***,flies with reduced Cht 10 function are unable to *** conclusion,Cht10 is essential for wing terminal differentiation and function.