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Pyrroloquinoline quinone regulates the redox status in vitro and in vivo of weaned pigs via the Nrf2/HO-1 pathway

journal of Animal Science and Biotechnology 2021年第4期12卷 1451-1467页

作者： Caiyun Huang Zijuan Fan Dandan Han Lee j.j.hnston Xi Ma Fenglai Wang State Key Lab of Animal Nutrition College of Animal Science&TechnologyChina Agricultural UniversityBeijing 100193China Swine Nutrition and Production West Central Research and Outreach CenterUniversity of MinnesotaMorrisMNUSA Department of Internal Medicine/Department of Biochemistry University of Texas Southwestern Medical CenterDallasTXUSA

Background:Oxidative stress is a main cause of piglet gut damage and *** quinone(PQQ),is a novel redox cofactor with antioxidant ***,the effect and mechanism that PQQ supplementation decreases oxidative injury in weaned pigs is not ***,the aim of this study is to confirm the effect of PQQ on regulating redox status in weaned pigs and the mechanism for antioxidant function by porcine intestinal epithelial cell line(IPEC-j2)challenged with H_(2)O_(2).Results:Experiment 1,144 Duroc×Landrace×Yorkshire pigs(weaned at 28 d)were allocated to four groups:received a basal diet(control)and diets supplemented with 0.15%,0.30%and 0.45%PQQ,*** d 28,growth performance,diarrhea incidence and redox factors were *** 2,IPEC-j2 were treated with or without PQQ in the presence or absence of H_(2)O_(2)for indicated time *** 3,IPEC-j2 were transfected with or without Nrf2 siRNA,then treated according to Experiment *** cell viability,redox factors,protein of tight junctions and Nrf2 pathway were *** vivo,PQQ supplementation demonstrated dose-related improvements in average daily gain,and gain to feed ratio(Linear Pj.j.num,and increased concentration of SOD in liver;0.3%PQQ supplementation decreased ileal and liver MDA concentration;and 0.15%PQQ supplementation decreased ileal MDA concentration(Pjunction proteins expression including ZO-1,ZO-2,Occludin and Claudin-1;and decreased ROS concentration and level of Caspase-3(Pbax and protein expression of nuclear Nrf2,***,Nrf2 knockdown by transfection with Nrf2 siRNA largely abrogated the positive effects of PQQ pretreatment on H_(2)O_

关键词： H_(2)O_(2) IPEC-j2 Nrf2 Oxidative stress Pyrroloquinoline quinone Weaned pig

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床旁D-二聚体检测在门诊患者排除静脉血栓栓塞的价值：一项诊断性荟萃分析

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英国医学杂志中文版 2009年第6期12卷 342-350页

作者： G j Geersing K j M janssen R Oudega L bax A W Hoes j B Reitsma K G M Moons 高晓方(译) 陈良安(校) 何耀(校) julius Centerfor Health Sciencesand Prirrary Care University MedicalCenter UtrechtUtrecht Netherland Kitasato ClinicalResearch Center Kitasato UniversityJapan Department ofClinical Epidemiologyarm Biostatistics Academic MedicalCenter Universityof AmsterdamAmsterdamNetherlands 解放军总医院

目的对目前排除静脉血栓栓塞的床旁D-二聚体检测的诊断准确性的证据进行评价。设计利用双变量回归检验变异来源，系统评价关于床旁D-二聚体检测的准确性的相关研究，并对敏感性和特异性进行评估。数据来源发表于1995年1月至2008年9月，... 详细信息

目的对目前排除静脉血栓栓塞的床旁D-二聚体检测的诊断准确性的证据进行评价。设计利用双变量回归检验变异来源，系统评价关于床旁D-二聚体检测的准确性的相关研究，并对敏感性和特异性进行评估。数据来源发表于1995年1月至2008年9月，并且于Medline和Embase中可检索到的关于床旁D-二聚体检测的诊断准确性的研究报告。评价方法纳入荟萃分析的研究需满足如下条件：对床旁D-二聚体检测与预先定义的静脉血栓栓塞的D-二聚体参考标准进行比较，人选患者为连续门诊病例，并且能够以此构建2×2表格。结果共有23项研究报告（病例总数为13959例，平均年龄为38—65岁，静脉血栓栓塞发病率为4％～51％）纳入荟萃分析。这些研究报告了两种床旁D-二聚体定性检测方法（SimpliREDD-二聚体（n=12）和Clearview Simplify D-二聚体（n=7））和两种床旁D-二聚体定量检测方法（CardiacD-二聚体（n=4）和Triage D-二聚体（n=2））。总体敏感性处于0．85（95％可信区间为0．78～0．90）至0．96（0．91～0．98）之间；总体特异性处于0．48（0．33～0．62）至0．74（0．96～0．78）之间。CardiacD-二聚体和TriageD-二聚体这两种定量检测方法评分最佳。结论在可疑静脉血栓栓塞的门诊患者中，床旁D-二聚体检测能够提供重要信息并指导治疗，在低风险患者（即临床决策规则评分较低的患者）中的价值尤为显著。

关键词： D-二聚体检测静脉血栓栓塞荟萃分析门诊患者床旁诊断性定量检测方法 Medline

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远端有瘘管的食管闭锁修补术后常规术后扩张是否优于症状出现后扩张——欧洲两小儿外科中心的结果比较

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世界核心医学期刊文摘（儿科学分册） 2005年第4期1卷 51-52页

作者： Koivusalo A. Turunen P. Rintala R. j. N. M. A. bax 肖莉 Willhelmina Children's Hospital University Medical Center Utrecht The Utrecht Netherlands

背景/目的:确定对远端有瘘管的食道闭锁(EADF),在修补术后进行常规扩张是否比保守观察,仅当症状出现时才扩张的效果好。方法:回顾在欧洲两个小儿外科中心100例EADF患儿的手术纪录[A(n=63),B(n=37)]。在A中心,吻合口扩张术只对有症状的... 详细信息

背景/目的:确定对远端有瘘管的食道闭锁(EADF),在修补术后进行常规扩张是否比保守观察,仅当症状出现时才扩张的效果好。方法:回顾在欧洲两个小儿外科中心100例EADF患儿的手术纪录[A(n=63),B(n=37)]。在A中心,吻合口扩张术只对有症状的病例实施,然而在B中心。

关键词：食管闭锁小儿外科扩张术常规扩张吻合口术后并发症 atresia dilatation 气管软化 anastomosis

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Chlorogenic acid complex(CGA7),standardized extract from green coffee beans exerts anticancer effects against cultured human colon cancer HCT-116 cells

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Food Science and Human Wellness 2017年第3期6卷 147-153页

作者： K.Gouthamchandra H.V.Sudeep B.j.Venkatesh K.Shyam Prasad Department of Biomedicinal Research R&D Centre for ExcellenceVidya Herbs Pvt.Ltd#14AJigani I PhaseBangalore 560105KarnatakaIndia

Coffee is commonly consumed beverage in the world and it has been suggested to have beneficial *** acids(CGAs)are main ingredient of coffee beans which has been extensively used in nutraceuticals and ***,various therapeutic effects of chlorogenic acids have been ***,there are limited studies to investigate its anticancer *** the present study,we have used chlorogenic acid complex(CGA7)a decaffeinated water soluble green coffee bean extract to evaluate its cytotoxic effect on human and mouse cancer cell lines by using different *** our results we found CGA7 treatment induces cell death in a dose and time dependent manner in different cancer cell ***,CGA7 induced apoptosis was characterized by DNA fragmentation,PARP-1 cleavage,caspase-9 activation,and down regulation of Bcl-2,an anti-apoptotic protein and up regulation of pro-apoptotic protein *** findings indicated that CGA7 complex a potent anticancer molecule found in green coffee beans could be a safe bioactive ingredient for prevention of cancer.

关键词： Apoptosis Chlorogenic acid complex PARP-1 Green coffee beans DNA fragmentation

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5-Fluorouracil-related enhancement of adenoviral infection is Coxsackievirus-adenovirus receptor independent and associated with morphological changes in lipid membranes

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World journal of Gastroenterology 2006年第32期12卷 5168-5174页

作者： Chiara Cabrele Mandy Vogel Pompiliu Piso Markus Rentsch josef Schroder Karl W jauch Hans j Schlitt Alexander Beham Department of Surgery Clinic of the Regensburg University Franz-Josef-Strauss-Allee 11D-93053RegensburgGermany

AIM： To evaluate the mechanism underlying the effects of 5-Fluorouracil （5-FU） on adenoviral infection. METHODS： Low and high Coxsackievirus-Adenovirus Receptor （CAR） expressing human colon carcinoma cell lines were treated with 5-FU and two El-deleted adenoviral constructs, one transferring GFP （Ad/CMV- GFP） the other bax （Ad/CEA-bax）. The number of infected cells were monitored by GFP expression. To evaluate the effects of 5-FU in a receptor free system, Ad/GFP were encapsulated in liposomes and treated with 5-FU. Ad/GFP release was estimated with PCR and infection of 293 cells with the supernatant. Electron microscopy of the Ad5-GFP-liposome complex was made to investigate morphological changes of the liposomes after 5-FU. RESULTS： Infection rates of all cell lines increased from 50% to 98% with emerging 5-FU concentrations. The enhanced viral uptake was independent of the CAR expression. Additionally, 5-FU treated liposomes released 2-2.5 times more adenoviruses. Furthermore, 5-FU- treated liposomes appeared irregular and porous-like. CONCLUSION： adenoviral uptake is enhanced in the presence of 5-FU irrespective of CAR and is associated with morphological changes in membranes making the combination of both a promising option in gene therapy.

关键词： 5-Fluorouracil Coxsackievirus-adenovirusreceptor Adenoviral infection Gene therapy

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二尖瓣和三尖瓣血流:应用回顾性瓣膜追踪3D速度编码MRI精确测量

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国际医学放射学杂志 2009年第1期32卷 71-71页

作者： j.j. Westenberg S.D. Roes N. Ajmone Marsan N.M. Binnendijk j. Doornbos j.j. bax 周丽娜 Department of Radiology Leiden University Medical Center Albinusdreef 2 2333 ZA Leiden the Netherlands

目的验证回顾性瓣膜追踪3D3个方向速度编码MRI对血流量的评估，并在健康志愿者和瓣膜返流病人中与传统2D单向速度编码MRI结果相比较。材料与方法病人和志愿者均知情同意，研究获得当地医学伦理委员会的批准。病人的数据随机选自2006... 详细信息

目的验证回顾性瓣膜追踪3D3个方向速度编码MRI对血流量的评估，并在健康志愿者和瓣膜返流病人中与传统2D单向速度编码MRI结果相比较。材料与方法病人和志愿者均知情同意，研究获得当地医学伦理委员会的批准。病人的数据随机选自2006年7月—2007年7月的MR研究数据库，为回顾性资料。3D3个方向速度编码MRI首先在体外验证，并与2D单向速度编码MRI进行比较。

关键词：速度编码瓣膜返流 MRI 血流量追踪三尖瓣二尖瓣医学伦理委员会

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Dietary N-carbamylglutamate and L-arginine supplementation improves redox status and suppresses apoptosis in the colon of intrauterine growth-retarded suckling lambs

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Animal Nutrition 2022年第4期11卷 359-368页

作者： Hao Zhang Yi Zheng Xia Zha Xiaoyun Liu Yi Ma juan j.Loor Mabrouk Elsabagh Mengzhi Wang Hongrong Wang Honghua jiang Laboratory of Metabolic Manipulation of Herbivorous Animal Nutrition College of Animal Science and TechnologyYangzhou UniversityYangzhou 225009China joint International Research Laboratory of Agriculture and Agri-Product Safety The Ministry of Education of ChinaYangzhou UniversityYangzhou 225009China Department of Animal Sciences and Division of Nutritional Sciences University of IllinoisUrbanaIL61801USA Department of Animal Production and Technology Faculty of Agricultural Sciences and TechnologiesNigde Omer Halisdemir UniversityNigde 51240Turkey Department of Nutrition and Clinical Nutrition Faculty of Veterinary MedicineKafrelsheikh UniversityKafrelsheikh 33516Egypt Department of Pediatrics Northern Jiangsu People’s HospitalClinical Medical CollegeYangzhou UniversityYangzhou 225001China

Previous studies have revealed that dietary N-carbamylglutamate(NCG)or L-arginine(Arg)improves small intestinal integrity and immune function in suckling Hu lambs that have experienced intrauterine growth retardation(IUGR).Whether these nutrients alter redox status and apoptosis in the colon of IUGR lambs is still *** study,therefore,aimed at investigating whether dietary supplementation of Arg or NCG alters colonic redox status,apoptosis and endoplasmic reticulum(ER)stress and the underlying mechanism of these alterations in IUGR suckling Hu ***-eight 7-d old Hu lambs,including 12 with normal birth weight(4.25±0.14 kg)and 36 with IUGR(3.01±0.12 kg),were assigned to 4 treatment groups(n=12 each;6 males and 6 females)for 3 *** treatment groups were control(CON),IUGR,IUGR+Arg and IUGR+*** to IUGR lambs,superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)content,as well as proliferation index,were higher(Pbax),apoptosis antigen 1(Fas),activating transcription factor 6(ATF6),caspase 3,and glucose-regulated protein 78(GRP78)were lower(P<0.05)while glutathione peroxidase 1(GPx1),Bcl-2 and catalase(CAT)levels were higher(P<0.05)in colonic tissue for IUGR+Arg and IUGR+NCG lambs compared with IUGR *** on our results,dietary NCG or Arg supplementation can improve colonic redox status and suppress apoptosis via death receptor-dependent,mitochondrial and ER stress pathways in IUGR suckling lambs.

关键词： Colon Endoplasmic reticulum stress IUGR lambs L-arginine N-carbamylglutamate Redox status

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水飞蓟素的肝脏保护作用研究(英文)

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中南大学学报（医学版） 2012年第1期37卷 6-10页

作者： Frank j.Burczynski 汪谷奇 David Nguyen 陈宇非 Howard j.Smith 巩跃文马尼托巴大学药学院加拿大马尼托巴温尼伯R3E OT5 马尼托巴大学医学院药理学与药物治疗系加拿大马尼托巴温尼伯R3E OT5 卡罗林医学中心美国北卡罗莱那州夏洛特28203 Howard j Smith联合公司澳大利亚墨尔本VIC3000

目的:研究水飞蓟素对人正常肝细胞(Chang细胞株)的保护作用,尤其是其抗氧化活性和对细胞抗凋亡的保护作用。方法:采用400μmol/L H2O2处理Chang细胞20 min后,通过二氯荧光黄(dichlorofluorescein,DCF)荧光强度检测细胞内自由基水平,并... 详细信息

目的:研究水飞蓟素对人正常肝细胞(Chang细胞株)的保护作用,尤其是其抗氧化活性和对细胞抗凋亡的保护作用。方法:采用400μmol/L H2O2处理Chang细胞20 min后,通过二氯荧光黄(dichlorofluorescein,DCF)荧光强度检测细胞内自由基水平,并检测了细胞活性及细胞内bax表达水平和ATP水平。结果:水飞蓟素明显降低了DCF荧光信号,显示细胞内自由基生成减少。MTT实验结果显示水飞蓟素可增强细胞活性,增加细胞内ATP水平,而减少促凋亡蛋白bax的转录和表达水平。结论:水飞蓟素可抑制Bas表达,具有保护细胞抗氧化应激所致自由基损伤的作用。水飞蓟素可作为治疗特异性肝脏疾病的有效辅助药物。

关键词：水飞蓟素肝脏疾病肝脏保护作用凋亡

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正方观点：β-受体阻滞剂适用于行非心脏手术的心脏病患者

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麻醉与镇痛 2008年第1期4卷 11-13页

作者： Olaf Schouten, MD jeroen j. bax, MD, FESC Martin Dunkelgrun, MD Harm H. H. Feringa, MD Don Poldermans, MD, FESC 陈涵(译) 姚尚龙(校) Department of Vascular Surgery Department of Anesthesiology Erasmus University Medical Center Rotterdam the Netherlands Department of Cardiology Leiden University Medical Center Leiden the Netherlands 不详

据估计，每年行非心脏手术的1亿成人中，约有50万患者（0．5％）死于围手术期心脏并发症。Lee等曾报道，非心脏手术患者手术后心肌梗死发生率约为1．1％，即每年全世界有110万这类患者发生心肌梗死。尽管围手术期心肌梗死的病理生理变... 详细信息

据估计，每年行非心脏手术的1亿成人中，约有50万患者（0．5％）死于围手术期心脏并发症。Lee等曾报道，非心脏手术患者手术后心肌梗死发生率约为1．1％，即每年全世界有110万这类患者发生心肌梗死。尽管围手术期心肌梗死的病理生理变化尚未完全明确，但与非手术情况下的心肌梗死相似，两者均涉及冠状动脉粥样斑块破裂，导致血栓形成及继发性血管栓塞。斑块破裂的发生率增加可能来源于大手术之后的应激反应增强，这些应激反应包括交感兴奋导致的对动脉斑块的垂直应力增加，血管反应性增高引起的血管痉挛、纤溶活性降低、血小板活化以及血液高凝；交感张力的升高进一步增加心肌氧耗（如心动过速和心肌收缩力增强），导致心肌氧供和氧耗失衡，如持续时间较长，将会导致心肌梗死。至少有两项研究表明，在所有致死性围手术期心肌梗死病例中，50％的患者存在冠状动脉粥样斑块破裂和血栓形成，而在其余50％的患者中则观察到持续的氧供需失衡。这些研究是通过无创性检查（冠状动脉造影以及尸检）来评价围手术期心肌梗死的病理生理学改变的。

关键词：非心脏手术患者 β-受体阻滞剂心脏病患者动脉粥样斑块破裂冠状动脉造影心肌梗死病理生理学改变围手术期

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Pien Tze Huang inhibits tumor cell proliferation and promotes apoptosis via suppressing the STAT3 pathway in a colorectal cancer mouse model

Pien Tze Huang inhibits tumor cell proliferation and promote...

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2012·中国医师协会中西医结合医师大会第三次会议

作者： Aling Shen Thomas j.sferra Departments of Biochemistry and Molecular Biology The University of Oklahoma Health Sciences Center

Signal transducer and activator of transcription 3(STAT3) plays a critical role in cell survival and *** activation of STAT3 is strongly correlated with pathogenesis of various types of malignant tumor including colorectal cancer(CRC),and therefore becomes a major focus in the development of anti-cancer *** Tze Huang(PZH),a well-known traditional Chinese formula prescribed 450 years ago in the Ming Dynasty,has been demonstrated to be clinically effective in the treatment of ***,the precise mechanism of its anti-cancer activity remains largely unknown. In present study we evaluated the efficacy of PZH against tumor growth in vivo in the CRC mouse xenograft model,and investigated the underlying molecular *** found that administration of PZH reduced tumor volume and tumor weight but had no effect on body weight gain in CRC mice, demonstrating that PZH can inhibit colon cancer growth in vivo without apparent adverse *** also observed that PZH treatment inhibited the phosphorylation level of STAT3 in tumor ***, the inhibitory effect of PZH on STAT3 activation resulted in the up-regulation of bax/Bcl-2 ratio as well as down-regulation of Cyclin D1 and CDK4 expression,leading to the induction of apoptosis as well as the inhibition of cell *** results suggest that promotion of cancer cell apoptosis and inhibition of proliferation via suppression of STAT3 pathway might be one of the mechanisms by which PZH treats colorectal cancer.

关键词： Pien Tze Huang Chinese medicine colorectal cancer STAT3 pathway apoptosis proliferation

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