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Chitosan@Puerarin hydrogel for accelerated wound healing in diabetic subjects by miR-29ab1 mediated inflammatory axis suppression

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Bioactive Materials 2023年第1期19卷 653-665页

作者： Xiaoling Zeng baohui chen Luping Wang Yingxiao Sun Zhao Jin Xuanyong Liu Liping Ouyang Yun Liao Department of Pharmacy Tongren HospitalShanghai Jiao Tong University School of MedicineShanghai200336China State Key Laboratory of High Performance Ceramics and Superfine Microstructure Shanghai Institute of CeramicsChinese Academy of SciencesShanghai200050China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing100049China Hongqiao International Institute of Medicine Shanghai Jiao Tong University School of MedicineShanghai200336China School of Chemistry and Materials Science Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of Sciences1 Sub-lane XiangshanHangzhou310024China Cixi Center of Biomaterials Surface Engineering Shanghai Institute of CeramicsChinese Academy of SciencesNingbo315300China

Wound healing is one of the major global health concerns in patients with diabetes.Overactivation of pro-inflammatory M1 macrophages is associated with delayed wound healing in diabetes.miR-29ab1 plays a critical role in diabetes-related macrophage inflammation.Hence,inhibition of inflammation and regulation of miR-29 expression have been implicated as new points for skin wound healing.In this study,the traditional Chinese medicine,puerarin,was introduced to construct an injectable and self-healing chitosan@puerarin(C@P)hydrogel.The C@P hydrogel promoted diabetic wound healing and accelerated angiogenesis,which were related to the inhibition of the miR-29 mediated inflammation response.Compared to healthy subjects,miR-29a and miR-29b1 were ectopically increased in the skin wound of the diabetic model,accompanied by upregulated M1-polarization,and elevated levels of IL-1βand TNF-α.Further evaluations by miR-29ab1 knockout mice exhibited superior wound healing and attenuated inflammation.The present results suggested that miR-29ab1 is essential for diabetic wound healing by regulating the inflammatory response.Suppression of miR-29ab1 by the C@P hydrogel has the potential for improving medical approaches for wound repair.

关键词： Hydrogel Diabetic skin wound healing Macrophages miR-29ab1

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Puerarin@Chitosan composite for infected bone repair through mimicking the bio-functions of antimicrobial peptides

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Bioactive Materials 2023年第3期21卷 520-530页

作者： Liping Ouyang baohui chen Xingdan Liu Donghui Wang Yang Li Yun Liao Kelvin W.K.Yeung Xuanyong Liu State Key Laboratory of High Performance Ceramics and Superfine Microstructure Shanghai Institute of CeramicsChinese Academy of SciencesShanghai200050China Hongqiao International Institute of edicine Shanghai Jiao Tong University School of MedicineShanghai200336China Cixi Center of Biomaterials Surface Engineering Ningbo315300China Department of Computational Biology for Individualised Medicine Centre for Individualised Infection Medicine(CiiM)&TWINCOREJoint Ventures Between the Helmholtz-Centre for Infection Research(HZI)and the Hannover Medical School(MHH)HannoverGermany Shenzhen Key Laboratory for Innovative Technology in Orthopaedic Trauma Guangdong Engineering Technology Research Center for Orthopaedic Trauma RepairDepartment of Orthopaedics and TraumatologyThe University of Hong Kong Shenzhen HospitalShenzhenChina

It is important to eliminate lipopolysaccharide(LPS)along with killing bacteria in periprosthetic joint infection(PJI)therapy for promoting bone repair due to its effect to regulate macrophages response.Although natural antimicrobial peptides(AMPs)offer a good solution,the unknown toxicity,high cost and exogenetic immune response hamper their applications in clinic.In this work,we fabricated a nanowire-like composite material,named P@C,by combining chitosan and puerarin via solid-phase reaction,which can finely mimic the bio-functions of AMPs.Chitosan,serving as the bacteria membrane puncture agent,and puerarin,serving as the LPS target agent,synergistically destroy the bacterial membrane structure and inhibit its recovery,thus endowing P@C with good antibacterial property.In addition,P@C possesses good osteoimmunomodulation due to its ability of LPS elimination and macrophage differentiation modulation.The in vivo results show that P@C can inhibit the LPS induced bone destruction in the Escherichia coli infected rat.P@C exhibits superior bone regeneration in Escherichia coli infected rat due to the comprehensive functions of its superior antibacterial property,and its ability of LPS elimination and immunomodulation.P@C can well mimic the functions of AMPs,which provides a novel and effective method for treating the PJI in clinic.

关键词： Osteoimmunomodulation Antibacterial Chitosan Puerarin Lipopolysaccharide

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Recent advances in CRISPR research

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Protein & Cell 2020年第11期11卷 786-791页

作者： baohui chen Yuyu Niu Haoyi Wang Kejian Wang Hui Yang Wei Li Department of Cell Biology and Bone Marrow Transplantation Center of the First Affiliated HospitalZhejiang University School of MedicineHangzhou 310058China Yunnan Key Laboratory of Primate Biomedical Research Institute of Primate Translational MedicineKunming University of Science and TechnologyKunming 650500China State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China State Key Laboratory of Rice Biology China National Rice Research InstituteChinese Academy of Agricultural SciencesHangzhou 310006China Institute of Neuroscience State Key Laboratory of NeuroscienceKey Laboratory of Primate NeurobiologyCAS Center for Excellence in Brain Science and Intelligence TechnologyShanghai Research Center for Brain Science and Brain-Inspired IntelligenceShanghai Institutes for Biological SciencesChinese Academy of SciencesShanghai 200031China

The clustered regularly interspaced short palindromic repeats(CRISPR)technology has revolutionized life sciences and developed rapidly.Here,we highlight the recent advances in development and application of CRISPR technologies,including the discovery of novel CRISPR systems,CRISPR base editing and imaging,and the applications of CRISPR in plant breeding,animal breeding,disease modeling and biotherapy.

关键词： CRISPR breeding advances

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气凝胶毡抑制磷酸铁锂电池模组热失控蔓延特性研究

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消防科学与技术 2024年第1期43卷 5-11页

作者：陈宝辉邓捷吴传平周天念国网湖南省电力公司防灾减灾中心电网防灾减灾全国重点实验室湖南长沙410007

热失控安全问题是制约锂离子电池储能技术发展的瓶颈。热阻隔是抑制电池热失控蔓延的技术途径之一。本文采用55 Ah磷酸铁锂储能电池模组,对比研究不安放气凝胶毡,以及安放不同厚度气凝胶毡对磷酸铁锂电池模组热失控蔓延特性的影响。研... 详细信息

热失控安全问题是制约锂离子电池储能技术发展的瓶颈。热阻隔是抑制电池热失控蔓延的技术途径之一。本文采用55 Ah磷酸铁锂储能电池模组,对比研究不安放气凝胶毡,以及安放不同厚度气凝胶毡对磷酸铁锂电池模组热失控蔓延特性的影响。研究表明,气凝胶毡可有效抑制磷酸铁锂电池热失控蔓延;气凝胶毡越厚,阻隔热蔓延的效果越好,0.5、1.2、3.0 mm厚的气凝胶毡可分别提升电池模组热阻隔效率75.80%、88.81%和299.92%。研究结论可为储能锂离子电池热失控防治提供参考。

关键词：锂离子电池磷酸铁锂电池气凝胶毡热蔓延热失控

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风力机叶片吸能防冰涂料的实验研究

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湖南电力 2024年第2期44卷 129-133页

作者：潘碧宸陈宝辉唐壮电网防灾减灾全国重点实验室湖南长沙410129

针对风力机叶片在冬季易发生覆冰的问题,研发了一种具备吸能功能的风力机叶片防冰涂料,可以通过吸收一定波长的光发热,提升涂料表面的温度,使涂料与表面覆冰之间的界面发生融化,从而让冰层从风力机叶片表面脱落,实现叶片防冰。通过反应... 详细信息

针对风力机叶片在冬季易发生覆冰的问题,研发了一种具备吸能功能的风力机叶片防冰涂料,可以通过吸收一定波长的光发热,提升涂料表面的温度,使涂料与表面覆冰之间的界面发生融化,从而让冰层从风力机叶片表面脱落,实现叶片防冰。通过反应制备得到吸能颜料,并与成膜基体、耐磨填料等材料混合,制备得到吸能涂料;对比涂覆该涂料的叶片与普通叶片的覆冰情况,发现该涂料可降低叶片表面覆冰速率12.8%,缩短覆冰时间50%;此外,通过开展耐磨性实验,发现该涂料耐磨性为2.81 L/μm,优于叶片原本涂料耐磨性(1.95 L/μm)。以上结果表明,该涂料可降低叶片表面覆冰速率,缩短叶片表面覆冰时间,且耐磨性更好,使用寿命更长。

关键词：风力机叶片吸能涂料覆冰耐磨性

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特高压换流变压器含水系灭火剂的水喷雾系统灭火试验研究

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湖南电力 2024年第2期44卷 44-49页

作者：陈宝辉吴传平周天念刘毓李波国网湖南省电力有限公司防灾减灾中心(电网防灾减灾全国重点实验室) 湖南长沙410129 湖南省防灾科技有限公司湖南长沙410129

采用1∶1全尺寸特高压换流变压器实体火模型,开展添加水系灭火剂的水喷雾灭火系统灭特高压换流变压器全尺寸实体火的试验研究,提出已安装水喷雾灭火系统换流站的消防改造方案,以及水喷雾灭火系统抗烧和抗爆能力提升的解决方案,提升特高... 详细信息

采用1∶1全尺寸特高压换流变压器实体火模型,开展添加水系灭火剂的水喷雾灭火系统灭特高压换流变压器全尺寸实体火的试验研究,提出已安装水喷雾灭火系统换流站的消防改造方案,以及水喷雾灭火系统抗烧和抗爆能力提升的解决方案,提升特高压换流变压器消防能力。结果表明,在美国防火协会标准的水喷雾灭火系统设计标准下,添加6%水系灭火剂的水喷雾灭火系统仅34 s就扑灭了全尺寸特高压换流变压器实体火,验证了添加水系灭火剂的水喷雾灭火系统的灭火能力。

关键词：特高压换流变压器水系灭火剂水喷雾灭火换流站

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对青少年近视佩戴角膜塑形镜患者实施优质护理的价值探讨

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中外医疗 2024年第12期43卷 183-186页

作者：卢玉梁晓燕陈宝辉屈坤鹏甘肃省人民医院眼视光学中心甘肃兰州730000 甘肃省人民医院普外科甘肃兰州730000

目的探讨对青少年近视佩戴角膜塑形镜患者采取优质护理的意义。方法随机选取2021年10月—2023年10月甘肃省人民医院接诊的70例近视佩戴角膜塑形镜患者为研究对象,按照随机数表法分成对照组与观察组。对照组35例采用常规护理,观察组35例... 详细信息

目的探讨对青少年近视佩戴角膜塑形镜患者采取优质护理的意义。方法随机选取2021年10月—2023年10月甘肃省人民医院接诊的70例近视佩戴角膜塑形镜患者为研究对象,按照随机数表法分成对照组与观察组。对照组35例采用常规护理,观察组35例采用优质护理。对比两组患者护理前后裸眼视力、屈光度数差异及并发症。结果护理前和护理后7 d时,观察组裸眼视力、屈光度数与对照组对比,差异无统计学意义(P均>0.05);观察组护理后30、60 d时,裸眼视力、屈光度数更优,差异有统计学意义(P均<0.05)。观察组并发症发生率5.71%低于对照组,差异有统计学意义(χ^(2)=5.285,P<0.05)。结论青少年近视佩戴角膜塑形镜时,给予优质护理可进一步改善近视患者的裸眼视力和屈光度数,并降低角膜上皮损伤等并发症的发生,安全性更高,患者接受度更高。

关键词：青少年近视角膜塑形镜优质护理裸眼视力

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Oncogenic β-catenin-driven liver cancer is susceptible to methotrexate-mediated disruption of nucleotide synthesis

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Chinese Medical Journal 2024年第2期137卷 181-189页

作者： Fangming Liu Yuting Wu baohui Zhang Shuhui Yang Kezhuo Shang Jie Li Pengju Zhang Weiwei Deng Linlin chen Liang Zheng Xiaochen Gai Hongbing Zhang Department of Physiology State Key Laboratory of Common Mechanism Research for Major DiseasesHaihe Laboratory of Cell EcosystemInstitute of Basic Medical Sciences and School of Basic MedicineChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100005China Department of Physiology Institute of Basic Medical Sciences and School of Basic MedicineGraduate School of Peking Union Medical CollegeChinese Academy of Medical SciencesBeijing100005China Department of Physiology School of Life ScienceChina Medical UniversityShenyangLiaoning 110122China School of Life Sciences Westlake UniversityHangzhouZhejiang 310024China Key Laboratory of Pediatric Hematology and Oncology Ministry of HealthInstitute of Pediatric Translational MedicineShanghai Children’s Medical CenterShanghai Jiao Tong School of MedicineShanghai 200127China

Background:Liver cancer is largely resistant to chemotherapy.This study aimed to identify the effective chemotherapeutics forβ-catenin-activated liver cancer which is caused by gain-of-function mutation of catenin beta 1(CTNNB1),the most frequently altered proto-oncogene in hepatic neoplasms.Methods:Constitutiveβ-catenin-activated mouse embryonic fibroblasts(MEFs)were established by deleting exon 3(β-catenin^(Δ(ex3)/+)),the most common mutation site in CTNNB1 gene.A screening of 12 widely used chemotherapy drugs was conducted for the ones that selectively inhibitedβ-catenin^(Δ(ex3)/+)but not for wild-type MEFs.Untargeted metabolomics was carried out to examine the alterations of metabolites in nucleotide synthesis.The efficacy and selectivity of methotrexate(MTX)onβ-catenin-activated human liver cancer cells were determined in vitro.Immuno-deficient nude mice subcutaneously inoculated withβ-catenin wild-type or mutant liver cancer cells and hepatitis B virus(HBV);β-catenin^(lox(ex3)/+)mice were used,respectively,to evaluate the efficacy of MTX in the treatment ofβ-catenin mutant liver cancer.Results:MTX was identified and validated as a preferential agent against the proliferation and tumor formation ofβ-catenin-activated cells.Boosted nucleotide synthesis was the major metabolic aberration inβ-catenin-active cells,and this alteration was also the target of MTX.Moreover,MTX abrogated hepatocarcinogenesis of HBV;β-catenin^(lox(ex3)/+)mice,which stimulated concurrent Ctnnb1-activated mutation and HBV infection in liver cancer.Conclusion:MTX is a promising chemotherapeutic agent forβ-catenin hyperactive liver cancer.Since repurposing MTX has the advantages of lower risk,shorter timelines,and less investment in drug discovery and development,a clinical trial is warranted to test its efficacy in the treatment ofβ-catenin mutant liver cancer.

关键词： Liver cancer β-catenin Methotrexate Nucleotide synthesis Chemotherapy

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J-family genes redundantly regulate flowering time and increase yield in soybean

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The Crop Journal 2024年第3期12卷 944-949页

作者： Haiyang Li Zheng chen Fan Wang Hongli Xiang Shuangrong Liu Chuanjie Gou Chao Fang Liyu chen Tiantian Bu Fanjiang Kong Xiaohui Zhao baohui Liu Xiaoya Lin Guangdong Key Laboratory of Plant Adaptation and Molecular Design/Guangzhou Key Laboratory of Crop Gene Editing/Innovative Center of Molecular Genetics and Evolution School of Life SciencesGuangzhou UniversityGuangzhou 510006GuangdongChina

Soybean(Glycine max)is a short-day crop whose flowering time is regulated by photoperiod.The longjuvenile trait extends its vegetative phase and increases yield under short-day conditions.Natural variation in J,the major locus controlling this trait,modulates flowering time.We report that the three J-family genes influence soybean flowering time,with the triple mutant Guangzhou Mammoth-2 flowering late under short days by inhibiting transcription of E1-family genes.J-family genes offer promising allelic combinations for breeding.

关键词： Soybean Flowering time Yield J-family genes

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Dt1 inhibits SWEET-mediated sucrose transport to regulate photoperiod-dependent seed weight in soybean

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Molecular Plant 2024年第3期17卷 496-508页

作者： Xiaoming Li Zhonghui chen Haiyang Li Lin Yue Cuirong Tan Hongjie Liu Yilong Hu Yuhua Yang Xiani Yao Lingping Kong Xiang Huangi Bin Yu Chunyu Zhang Yuefeng Guan baohui Liu Fanjiang Kong Xingliang Hou Guangdong Provincial Key Laboratory of Applied Botany&State Key Laboratory of Plant Diversity and Specialty Crops South China Botanical GardenChinese Academyof SciencesGuangzhou510650China Guangdong Provincial Key Laboratory of Plant Adaptation and Molecular Design Guangzhou Key Laboratory of Crop Gene EditingInnovative Center of Molecular Genetics and EvolutionSchool of Life SciencesGuangzhou UniversityGuangzhou 510006China University of Chinese Academy of Sciences Beijing 100049China College of Agronomy and Biotechnology China Agricultural UniversityBeijing 100193China

Soybean is a photoperiod-sensitive short-day crop whose reproductive period and yield are markedly affected by day-length changes.Seed weight is one of the key traits determining the soybean yield;how-ever,the prominent genes that control the final seed weight of soybean and the mechanisms underlying the photoperiod's effect on this trait remain poorly understood.In this study,we identify SwW19 as a major locus controlling soybean seed weight by QTL mapping and determine Dt1,an orthologous gene of Arabidopsis TFL1 that is known to govern the soybean growth habit,as the causal gene of the SW19 locus.We showed that Dt1 is highly expressed in developing seeds and regulates photoperiod-dependent seed weight in soybean.Further analyses revealed that the Dt1 protein physically interacts with the sucrose transporter GmSWEET10a to negatively regulate the import of sucrose from seed coat to the embryo,thus modulating seed weight under long days.However,Dt1 does not function in seed development under short days due to its very low expression.Importantly,we discovered a novel natural allelic variant of Dt1(H4 haplotype)that decouples its pleiotropic effects on seed size and growth habit;i.e.,this variant remains functional in seed development but fails to regulate the stem growth habit of soybean.Collectively,our findings provide new insights into how soybean seed development responds to photoperiod at different latitudes,offering an ideal genetic component for improving soybean's yield by manipulating its seed weightandgrowth habit.

关键词： soybean seed weight photoperiod dt1 sucrose transport

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