Puerarin@Chitosan composite for infected bone repair through mimicking the bio-functions of antimicrobial peptides

作     者：Liping Ouyang Baohui Chen Xingdan Liu Donghui Wang Yang Li Yun Liao Kelvin W.K.Yeung Xuanyong Liu 

作者机构：State Key Laboratory of High Performance Ceramics and Superfine MicrostructureShanghai Institute of CeramicsChinese Academy of SciencesShanghai200050China Hongqiao International Institute of edicineShanghai Jiao Tong University School of MedicineShanghai200336China Cixi Center of Biomaterials Surface EngineeringNingbo315300China Department of Computational Biology for Individualised MedicineCentre for Individualised Infection Medicine(CiiM)&TWINCOREJoint Ventures Between the Helmholtz-Centre for Infection Research(HZI)and the Hannover Medical School(MHH)HannoverGermany Shenzhen Key Laboratory for Innovative Technology in Orthopaedic TraumaGuangdong Engineering Technology Research Center for Orthopaedic Trauma RepairDepartment of Orthopaedics and TraumatologyThe University of Hong Kong Shenzhen HospitalShenzhenChina 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2023年第21卷第3期

页      面：520-530页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）] 

基　　金：National Natural Science Foundation of China(U21A20100,32000938) Science and Technology Commission of Shanghai Municipality,China(19JC1415500,20ZR1465000) Shenzhen Science and Technology Funding(JCYJ20210324120009026) S&T Innovation 2025 Major Special Program of Ningbo(2018B10040)are acknowledged. 

主　　题：Osteoimmunomodulation Antibacterial Chitosan Puerarin Lipopolysaccharide 

摘      要：It is important to eliminate lipopolysaccharide(LPS)along with killing bacteria in periprosthetic joint infection(PJI)therapy for promoting bone repair due to its effect to regulate macrophages response.Although natural antimicrobial peptides(AMPs)offer a good solution,the unknown toxicity,high cost and exogenetic immune response hamper their applications in clinic.In this work,we fabricated a nanowire-like composite material,named P@C,by combining chitosan and puerarin via solid-phase reaction,which can finely mimic the bio-functions of AMPs.Chitosan,serving as the bacteria membrane puncture agent,and puerarin,serving as the LPS target agent,synergistically destroy the bacterial membrane structure and inhibit its recovery,thus endowing P@C with good antibacterial property.In addition,P@C possesses good osteoimmunomodulation due to its ability of LPS elimination and macrophage differentiation modulation.The in vivo results show that P@C can inhibit the LPS induced bone destruction in the Escherichia coli infected rat.P@C exhibits superior bone regeneration in Escherichia coli infected rat due to the comprehensive functions of its superior antibacterial property,and its ability of LPS elimination and immunomodulation.P@C can well mimic the functions of AMPs,which provides a novel and effective method for treating the PJI in clinic.