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Development of a Polyclonal Antibody-based AC-ELISA and Its Comparison with PCR for Diagnosis of Canine Parvovirus Infection

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Virologica Sinica 2010年第5期25卷 352-360页

作者： Manoj kumar Sukdeb nandi Sunil Chidri Virology Laboratory Centre for Animal Disease Research and DiagnosisIndian Veterinary Research Institute

A polyclonal antibody-based antigen-capture ELISA （AC-ELISA） has been developed for detection of Canine parvovirus （CPV） antigens in faecal samples of dogs. The assay uses rabbit anti-CPV polyclonal antibody as the capture antibody, guinea pig anti-CPV polyclonal antibody as tracing antibody and anti-guinea pig HRPO conjugate as the detection system. The optimum dilution of the capture antibody and the tracing antibody capable of detecting the CPV-2 antigens was found to be 1：1 600 and 1：400, respectively, in the check-board titration. In this study, a total of 152 samples （129 faecal samples and 23 cell culture supernatant） were tested both by AC-ELISA and by polymerase chain reaction （PCR）. Of the samples tested, 69 and 78 samples were found positive by AC-ELISA and PCR, respectively. The AC-ELISA had relative sensitivity, relative specificity and accuracy of 88.4%, 100.0% and 91.4% respectively. The analytical sensitivity of AC-ELISA was estimated to be 102.8 TCID50/mL whereas PCR sensitivity was 100.8 TCIDs0/mL. The AC-ELISA is a simple, quick and reliable method for screening large numbers of faecal samples of dogs suspected of CPV infection.

关键词： Canine parvovirus (CPV) Polyclonal antibody Antigen-capture ELISA(AC-ELISA)

Effect of Hydro-ethanolic (1:1) Extract of Stephania Hernandifolia and Achyranthes Aspera in Composite Manner on Testicular Activity in Male Albino Rat:A Dose Dependent Analysis

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Journal of Reproduction and Contraception 2012年第4期23卷 209-221页

作者： Daniel Paul kazi Monjur Ali Debidas Ghosh Dilip k.nandi Hanéne Chenni Mohamed M.Trabelsi Department of Bio-Medical Laboratory Science & Management (UGC Innovative Programme Funded Department under Xth-5 years plan) Vidyasagar University Department of Physiology Raja N.L. Khan Women's College Laboratory of Histology and Embryology Faculty of Medicine

Objective To investigate the effect of hydro-ethanolic extraet of Stephania hernandifolia leaves and Aehyranthes aspera roots in composite manner at the ratio of 1：3 on testicular activity in male rats. Methods Rats were divided into 4 groups with 8 animals in each group. The control （group A） received 0. 5 ml of olive oil/100 g body weight orally, other three groups were treated with said extract orally at a dose of 0.4 mg/g （group B） or 0.8 mg/g （group C） or 1.6 mg/g body weight （group D） respectively for 28 d. On 29th day of experiment, the animals were sacrificed. Sperm concentrations in cauda epididymis and biochemical markers like testicular cholesterol, androgenic key enzyme activity, plasma testosterone level seminal fructose level, glutamate oxaloacetate transaminase （GOT） and glutamate pyruvate transaminase （GPT） activities and serum triglyceride levels were measured following standard methods. ResuIts Extract treated animals in all doses resulted in a significant （P〈O. 05）decrease in sperm concentration, testicular androgenic key enzyme activity, plasma testosterone and seminal vesicle fructose levels along with an increase in testieular cholesterol level Animals treated at a dose of O. 8 mg/g body weight showed more promising result without causing any metabolic toxicity compared with other doses. Histological study also supported the biochemical results. The minimum but most effective dose i.e. 0.8 mg/g body weight had an inhibitory effect on implantation focused here by mating *** The composite efficacy as male contraceptive extract of S. hernandifolia and A. aspera has a potent that may provide clues to the pharmaceutical industries for male contraceptive development

关键词： Achyranthes aspera Stephania hernandifolia testosterone seminiferous tubule

Effect of pyroxenite and olivine minerals as source of MgO in hematite pellet on improvement of metallurgical properties

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Journal of Central South University 2015年第9期22卷 3302-3310页

作者： Jagannath Pal Satadal Ghorai Bikash nandi Tapas Chakraborty Goutam Das T.Venugopalan CSIR-National Metallurgical Laboratory Tata Steel

Pelletization of hematite ore requires high fineness and very high induration temperature（~1325 ℃） owing to its poor diffusion bonding unlike magnetite ore. Further, high-alumina hematite pellets show very high reduction degradation index（RDI） during low temperature（500-650 ℃） reduction due to their volume expansion and lattice distortion. Noamundi（India） hematite ore contains very high Al2O3（2.3%） with adverse ratio of alumina to silica（~2） for which, it shows very high RDI. In this work, the acid pellets prepared from Noamundi ore fines of optimum Blaine fineness show good cold crushing strength（CCS）. However, it shows very high RDI（77%）. In order to reduce RDI, Mg O in form of two different gangue-containing fluxes, such as pyroxenite and olivine in varying quantities has been added. The optimum requirement and performance of these fluxes has been examined and compared. Both pyroxenite and olivine fluxed pellets show significant lowering of RDI（26% and 23%, respectively） and improvement of other properties, viz CCS, swelling indices etc with good reducibility（70%-77%）. Finally, a good quality acidic hematite pellet was developed from high-alumina ore without using any lime which is very important charge material in combination of basic sinter in blast furnace.

关键词： hematite pellet high-alumina ore reduction degradation index(RDI) pyroxenite flux olivine flux effect of MgO

In-Situ Hydrothermal Synthesis of Bi–Bi2O2CO3 Heterojunction Photocatalyst with Enhanced Visible Light Photocatalytic Activity

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Nano-Micro Letters 2017年第2期9卷 58-67页

作者： Prasenjit kar Tuhin kumar Maji Ramesh nandi Peter Lemmens Samir kumar Pal Department of Chemical Biological and Macromolecular Sciences S.N.Bose National Centre for Basic SciencesBlock JDSector ⅢSaltLakeKolkata 700106India Institute for Condensed Matter Physics TU BraunschweigMendelssohnstraße 338106 BrunswickGermany Laboratory for Emerging Nanometrology TU BraunschweigBrunswickGermany

Bismuth containing nanomaterials recently received increasing attention with respect to environmental applications because of their low cost, high stability and nontoxicity. In this work, Bi–Bi_2O_2CO_3 heterojunctions were fabricated by in-situ decoration of Bi nanoparticles on Bi_2O_2CO_3 nanosheets via a simple hydrothermal synthesis approach. X-ray diffraction(XRD), scanning electron microscopy(SEM), transmission electron microscopy(TEM) and high-resolution TEM(HRTEM) were used to confirm the morphology of the nanosheet-like heterostructure of the Bi–Bi_2O_2CO_3 composite. Detailed ultrafast electronic spectroscopy reveals that the in-situ decoration of Bi nanoparticles on Bi_2O_2CO_3 nanosheets exhibit a dramatically enhanced electron-hole pair separation rate, which results in an extraordinarily high photocatalytic activity for the degradation of a model organic dye, methylene blue(MB) under visible light illumination. Cycling experiments revealed a good photochemical stability of the Bi–Bi_2O_2CO_3 heterojunction under repeated irradiation. Photocurrent measurements further indicated that the heterojunction incredibly enhanced the charge generation and suppressed the charge recombination of photogenerated electron-hole pairs.

关键词： Bi nanoparticles Bi–Bi2O2CO3 nanosheets Heterojunction Hydrothermal method Charge separation Visible light photocatalytic activity

Epizootic, Endemic and Pandemic Zoonotic Viral Infections

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Journal of Biosciences and Medicines 2022年第3期10卷 90-96页

作者： Shravan Singh Rathore Hem Singh Gehlot Gyan Prakash Jayashree S. nandi Wildlife Institute of India GIB-CB Project Sam-Jaisalmer India Department of Zoology JNV University Jodhpur India Machiya Biological Park Post Office Saran Nagar Jodhpur India National Institute of Virology (Past) Pune India Department of Microbiology and Immunology Albert Einstein College of Medicine (Past) Bronx New York USA

More than 60% human infectious diseases have zoonotic origin. Cross species transmission of pathogens is a continuous, dynamic process that occurs throughout the world, giving rise to epizootic (temporary, limited infection), endemic (on-going infection limited to a defined geographic region) and pandemic viral infections (infection spreading to every part of the world) like the current COVID-19 pandemic, which depends on the existing conditions on the ground. In Nov 2021, sudden mortality of numerous migrating demoiselle cranes was reported from their resting site near Jodhpur, Rajasthan. The symptomatic cranes became gradually weak and were unable to fly. They eventually fell dead which caused concern locally, given the current prevailing SARS-CoV-2 pandemic situation the world over. By the end of Dec 2021, the number of cranes with mortality and morbidity reduced, making it a temporary “epizootic infection”. Molecular diagnosis carried out at a specialized laboratory identified the etiological agent to be the highly pathogenic Avian Influenza Virus H5N1 (HPAIV), which has been responsible for morbidity of avian species from different parts of the world. There was no report of spreading the H5N1 AIV infection from the infected migratory cranes to nearby chicken farms or pig farms for now. In the absence of vaccines against the highly pathogenic H5N1 AIVs, and the inherent ability of influenza viruses, both avian AIV and human IAVs to constantly mutate its envelope gene or the surface antigens, resulting from the error-prone nature of the viral RNA Polymerase enzyme are the roadblocks for development of a universal, broad-spectrum influenza vaccine. Even when such a universal vaccine against H5N1 is available, vaccinating a large number of wild migratory cranes would be difficult. However, it is possible and indeed necessary to vaccinate chickens in poultry farms and pigs in farms that raise pigs for human consumption.

关键词： Zoonotic Pathogens Natural Infection Anthroponosis Migratory Birds Across Countries Aquatic Demoiselle Cranes Viral Infectious Diseases

Targeting biosignatures of hyperglycemia and oxidative stress in diabetes comorbid depressive rats: effectiveness of hydroethanolic extract of the whole plant of Ludwigia octovalvis

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Traditional Medicine Research 2023年第2期8卷 13-20页

作者： Stuti Pandey Pawan kumar Pandey Alakh Niranjan Sahu Himanshu Verma Manmath kumar nandi Department of Medicinal Chemistry Faculty of AyurvedaInstitute of Medical SciencesBanaras Hindu UniversityVaranasi 221005Uttar PradeshIndia Department of Pharmaceutical Engineering&Technology Indian Institute of Technology Banaras Hindu UniversityVaranasi 221005Uttar PradeshIndia

Background:The prime objective of the current research was to evaluate the whole plant hydroalcoholic extract of Ludwigia octovalvis(HLO)against hyperglycemia,and oxidative stress biomarkers in rats induced with diabetes comorbid depression,diabetes comorbid depression(streptozotocin-nicotinamide+electric footshocks).Methods:2,2-Diphenyl-1-picrylhydrazyl assay of HLO versus ascorbic acid was *** of 200 and 400 mg/kg body weight/day HLO doses versus 25 mg/kg body weight/day metformin was studied through insulin,glucose,superoxide dismutase,lipid peroxidation,catalase,and behavioral assessment(forced swim and open field tests).Results:IC50 values of HLO and ascorbic acid were 33.52 and 27.86μg/mL *** the HLO doses showed intended results with respect to oxidative stress biomarkers in diabetes comorbid depression rats in comparison to *** field test showed better results for HLO in diabetes comorbid depression ***,hypoglycemic effects,and forced swim test performance of metformin was slightly higher than the 400 mg dose,followed by the 200 mg dose of *** gallate,gallic acid,β-sitosterol,and quercetin in HLO might resulted in attenuating diabetic as well as depression ***:Inhibition of glucosidase and lipase activity,and AMP-activated protein kinase phosphorylation might be the possible biochemical changes occurred in HLO treated rats.

关键词： 2,2-diphenyl-1-picrylhydrazyl assay brain homogenate insulin superoxide dismutase content catalase activity lipid peroxidation

Probability of a zoonotic spillover with seasonal variation

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Infectious Disease Modelling 2021年第1期6卷 514-531页

作者： Aadrita nandi Linda J.S.Allen Department of Mathematics and Statistics Texas Tech UniversityLubbockTX79409-1042USA Department of Microbiology and Immunology University of Michigan Medical SchoolAnn ArborMI48108-5260USA

Zoonotic infectious diseases are spread from animals to *** is estimated that over 60%of human infectious diseases are zoonotic and 75%of them are emerging *** majority of emerging zoonotic infectious diseases are caused by viruses including avian influenza,rabies,Ebola,coronaviruses and *** of infection from animals to humans depends on a complex transmission pathway,which is influenced by epidemiological and environmental *** this investigation,the focus is on direct transmission between animals and humans and the effects of seasonal variations on the transmission and recovery *** in transmission and recovery,besides being influenced by physiological processes and behaviors of pathogen and host,are driven by seasonal variations in temperature,humidity or rainfall.A new time-nonhomogeneous stochastic process is formulated for infectious disease spread from animals to humans when transmission and recovery rates are time-periodic.A branching process approximation is applied near the disease-free state to predict the probability of the first spillover event from animals to *** probability is a periodic function of the time when infection is introduced into the animal *** is shown that the highest risk of a spillover depends on a combination of animal to human transmission,animal to animal transmission and animal *** results are applied to a stochastic model for avian influenza with spillover from domestic poultry to humans.

关键词： Branching process Markov chain Seasonal fluctuations Spillover Zoonoses

Elevated basal intestinal mucosal cytokine levels in asymptomatic first-degree relatives of patients with Crohn's disease

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World Journal of Gastroenterology 2000年第1期6卷 49-52页

作者： Indaram AVk.nandi S Weissman S Lam S Bailey B Blumstein M Greenberg R Bank S Division of Gastroenterology Long Island Jewish Medical CenterAlbert Einstein College of MedicineNew YorkUSA

AIM To determine levels of cytokines incolonic mucosa of asymptomatic first degreerelatives of Crohn’s disease *** Cytokines(Interleukin(IL)1-Beta,IL-2,IL-5 and IL-8)were measured using ELISAin biopsy samples of normal looking colonicmucosa of first degree relatives of Crohn’sdisease patients(n = 9)and from normalcontrols(n = 10)with no family history ofCrohn’s *** Asymptomatic first degree relativesof patients with Crohn’s disease had significantlyhigher levels of basal intestinal mucosalcytokines(IL-2,IL-5 and IL-8)than *** these increased cytokinelevels serve as phenotypic markers for a geneticpredisposition to developing Crohns diseaselater on,or whether they indicate early(pre-clinical)damage has yet to be further *** Asymptomatic first degreerelatives of Crohn’s disease patients have higherlevels of cytokines in their normal-lookingintestinal mucosa compared to normal controls,This supports the hypothesis that increasedcytokines may be a cause or an early event inthe inflammatory cascade of Crohns disease andare not merely a result of the inflammatoryprocess.

关键词： Subject headings crohns disease cytokine/analysis intestinal mucosa

基于k个最近邻点的遗传程序的数字调制样式自动分类

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通信对抗 2011年第1期30卷 58-64页

作者： Muhammad Waqar Aslam asok. k.nandi 骆振兴曹国英 Department of Electrical Engineering & Electronics The University of Liverpool Brownlow Hill LiverpoolL693GJU.K.

在许多不同的民用和军用领域，调制样式自动分类都是一个非常有趣的问题。本文提出了一种基于k个最近邻点（k—Nearest Neighbor，kNN）的遗传程序（Genetic Programming，GP）算法。该算法采用了基于k个最近邻点的遗传程序，用来识别B... 详细信息

在许多不同的民用和军用领域，调制样式自动分类都是一个非常有趣的问题。本文提出了一种基于k个最近邻点（k—Nearest Neighbor，kNN）的遗传程序（Genetic Programming，GP）算法。该算法采用了基于k个最近邻点的遗传程序，用来识别BPSk、QPSk、16QAM和64QAM调制信号。该算法用高阶累积量作为输入特征，并使用一种分两个阶段的分类方法来改善分类精度。计算机仿真将所提出算法与已有的算法进行了比较，从而证明了该算法的优异性能。

关键词：数字调制自动分类遗传程序 k个最近邻点高阶累积量软件无线电检测与估计