Effect of Hydro-ethanolic (1:1) Extract of Stephania Hernandifolia and Achyranthes Aspera in Composite Manner on Testicular Activity in Male Albino Rat:A Dose Dependent Analysis

作     者：Daniel Paul Kazi Monjur Ali Debidas Ghosh Dilip K.Nandi Hanéne Chenni Mohamed M.Trabelsi Daniel Paul 1 , Kazi Monjur Ali 1 , Debidas Ghosh 1 , Dilip K. Nandi 2 , Hanéne Chenni 3 , Mohamed M. Trabelsi 3 1. Department of Bio-Medical Laboratory Science & Management (UGC Innovative Programme Funded Department under Xth-5 years plan) Vidyasagar University, Midnapore 721102, West Bengal, India 2. Department of Physiology, Raja N.L. Khan Women’s College, Midnapore 721102, West Bengal, India 3. Laboratory of Histology and Embryology, Faculty of Medicine, 4002 Sousse, Tunisia

作者机构：Department of Bio-Medical Laboratory Science & Management (UGC Innovative Programme Funded Department under Xth-5 years plan) Vidyasagar University Department of Physiology Raja N.L. Khan Women's College Laboratory of Histology and Embryology Faculty of Medicine 

出 版 物：《Journal of Reproduction and Contraception》 (生殖与避孕（英文版）)

年 卷 期：2012年第23卷第4期

页      面：209-221页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

主　　题：Achyranthes aspera Stephania hernandifolia testosterone seminiferous tubule 

摘      要：Objective To investigate the effect of hydro-ethanolic extraet of Stephania hernandifolia leaves and Aehyranthes aspera roots in composite manner at the ratio of 1：3 on testicular activity in male rats. Methods Rats were divided into 4 groups with 8 animals in each group. The control （group A） received 0. 5 ml of olive oil/100 g body weight orally, other three groups were treated with said extract orally at a dose of 0.4 mg/g （group B） or 0.8 mg/g （group C） or 1.6 mg/g body weight （group D） respectively for 28 d. On 29th day of experiment, the animals were sacrificed. Sperm concentrations in cauda epididymis and biochemical markers like testicular cholesterol, androgenic key enzyme activity, plasma testosterone level seminal fructose level, glutamate oxaloacetate transaminase （GOT） and glutamate pyruvate transaminase （GPT） activities and serum triglyceride levels were measured following standard methods. ResuIts Extract treated animals in all doses resulted in a significant （P〈O. 05）decrease in sperm concentration, testicular androgenic key enzyme activity, plasma testosterone and seminal vesicle fructose levels along with an increase in testieular cholesterol level Animals treated at a dose of O. 8 mg/g body weight showed more promising result without causing any metabolic toxicity compared with other doses. Histological study also supported the biochemical results. The minimum but most effective dose i.e. 0.8 mg/g body weight had an inhibitory effect on implantation focused here by mating *** The composite efficacy as male contraceptive extract of S. hernandifolia and A. aspera has a potent that may provide clues to the pharmaceutical industries for male contraceptive development