Development of a Polyclonal Antibody-based AC-ELISA and Its Comparison with PCR for Diagnosis of Canine Parvovirus Infection

作     者：Manoj Kumar Sukdeb Nandi Sunil Chidri 

作者机构：Virology LaboratoryCentre for Animal Disease Research and DiagnosisIndian Veterinary Research Institute 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2010年第25卷第5期

页      面：352-360页

核心收录：

学科分类：0710[理学-生物学] 090603[农学-临床兽医学] 1007[医学-药学(可授医学、理学学位)] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 09[农学] 0906[农学-兽医学] 

基　　金：The authors would like to acknowledge the Director  Indian Veterinary Research Institute (IVRI) for providing the facilities to carry out the work 

主　　题：ELISA法 犬细小病毒感染 多克隆抗体 PCR检测 AC 基础 诊断 聚合酶链反应 

摘      要：A polyclonal antibody-based antigen-capture ELISA (AC-ELISA) has been developed for detection of Canine parvovirus (CPV) antigens in faecal samples of dogs. The assay uses rabbit anti-CPV polyclonal antibody as the capture antibody, guinea pig anti-CPV polyclonal antibody as tracing antibody and anti-guinea pig HRPO conjugate as the detection system. The optimum dilution of the capture antibody and the tracing antibody capable of detecting the CPV-2 antigens was found to be 1:1 600 and 1:400, respectively, in the check-board titration. In this study, a total of 152 samples (129 faecal samples and 23 cell culture supernatant) were tested both by AC-ELISA and by polymerase chain reaction (PCR). Of the samples tested, 69 and 78 samples were found positive by AC-ELISA and PCR, respectively. The AC-ELISA had relative sensitivity, relative specificity and accuracy of 88.4%, 100.0% and 91.4% respectively. The analytical sensitivity of AC-ELISA was estimated to be 102.8 TCID50/mL whereas PCR sensitivity was 100.8 TCID50/mL. The AC-ELISA is a simple, quick and reliable method for screening large numbers of faecal samples of dogs suspected of CPV infection.