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Characterization of oral yeasts isolated from healthy individuals attended in different Colombian dental clinics

The Journal of Biomedical Research 2019年第5期33卷 333-342页

作者： Raul Eduardo Rivera alejandra Zuluaga Karen Arango Itzjak Kadar Paola andrea Pinillos Luis Fernando Montes Eugenia Catalina Cepeda Ernesto Gonzalez Pedro Antonio Alfonso andrea alejandra villalba Luis Fernando Casanova Adolfo Perez Armando Roa Martha Jhoana Arias Jorge Orlando Francisco Cuellar Lorena Pedraza Adiel Alberto Vasquez Blanca Lynne Suarez Beatriz LGomez Catalina De Bedout Luz Elena Cano Group of Investigation in Oral Health Faculty of DentistryAntonio Narino UniversityArmeniaQuindio 630001Colombia Medical and Experimental MycologyUnit Corporation for Biological Research(CIB)MedellinAntioquia 050034Colombia School of Medicine and Health Sciences Universidad del RosarioCarreraBogota 111221Colombia School of Microbiology University of AntioquiaMedellinAntioquia 050036Colombia.

The aim of this study was to identify the most frequent yeasts in the oral cavity of adult individuals without immune disorders and to associate the presence of these oral yeasts with different characteristics of each individual. Oral rinse samples were obtained from 96 healthy adults and cultured in Sabouraud dextrose agar media and CHROMagar. Yeasts were identified by sequencing the D1/D2 region of the 28 S rRNA gene. Probable association among the socio-demographic characteristics, body mass index, family and personal medical history,oral hygiene, tobacco and/or alcohol consumption habits and presence of oral fungi was analyzed. Contingency tables and logistic regression were employed to evaluate possible relationships between the presence of oral fungi and mixed colonization with these variables. 57.3% of the healthy individuals had oral yeasts and 21.8% had mixed colonization. The most prevalent yeasts were Candida albicans(52%), C. parapsilosis(17.9%), and ***(7.57%). Yeasts with most frequently mixed colonization were C. albicans and C. parapsilosis. No relationships were found among the variables analyzed. However, the presence of mixed colonization was related to the presence of dental prostheses(P<0.006), dental apparatuses(P=0.016) and O’Leary index(P=0.012). This is the first study that characterized oral yeasts in Colombian healthy individuals, determined the most prevalent oral yeasts C. albicans, C. parapsilosis and C. dublinensis and an association of mixed colonization with the use of dental prostheses and aparatology and poor hygiene.

关键词： oral yeast Candida species microbial epidemiology

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The W3C Data Catalog Vocabulary,Version 2:Rationale,Design Principles,and Uptake

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Data Intelligence 2024年第2期6卷 457-487页

作者： Riccardo Albertoni David Browning Simon Cox alejandra N.Gonzalez-Beltran andrea Perego Peter Winstanley Istituto di Matematica Applicata e Tecnologie Informatiche"Enrico Magenes" Consiglio Nazionale delle Ricerche(IMATI-CNR)GenoaItaly Independent Consultant United Kingdom LandandWater CSiROMelbourneAustralia Scientific Computing Rutherford Appleton LaboratoryScience and Technology Facilities CouncilHarwell Cam-pusOx110QXUnited Kingdom European Parliament Luxembourg Semantic Arts Fort CollinsCO80524USA

DCAT is an RDF vocabulary designed to facilitate interoperability between data catalogs published on the *** its first release in 2014 as a W3C Recommendation,DCAT has seen a wide adoption across communities and domains,particularly in conjunction with implementing the FAIR data principles(forfindable,accessible,interoperable and reusable data).These implementation experiences,besides demonstrating the fitness of DCAT to meet its intended purpose,helped identify existing issues and ***,over the last few years,additional requirements emerged in data catalogs,given the increasing practice of documenting not only datasets but also data services and *** paper illustrates the new version of DCAT,explaining the rationale behind its main revisions and extensions,based on the collected use cases and requirements,and outlines the issues yet to be addressed in future versions of DCAT.

关键词： Data Catalogs Interoperability Discoverability FAIR data

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A Saccharomyces cerevisiae RC016-based feed additive reduces liver toxicity,residual aflatoxin B1 levels and positively influences intestinal morphology in broiler chickens fed chronic aflatoxin B1-contaminated diets

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Animal Nutrition 2020年第1期6卷 31-38页

作者： Valeria Poloni alejandra Magnoli Analía Fochesato andrea Cristofolini Matias Caverzan Cecilia Merkis Maríana Montenegro Lilia Cavaglieri Departamento de Microbiología e Inmunología Universidad Nacional de Río CuartoRío CuartoArgentina Consejo Nacional de Investigaciones Científicas y Tecnólogicas(CONICET) Buenos AiresArgentina Departamento de Microscopía Electrónica Universidad Nacional de Río CuartoRío CuartoArgentina Centro de Investigaciones y Transferencia Villa María Universidad Nacional de Villa MaríaVilla MaríaArgentina

The present study was conducted to investigate the ability of Saccharomyces cerevisiae RC016(Sc)-based feed additive to reduce liver toxicity,residual aflatoxin B1(AFB1)levels and influence intestinal structure in broiler chickens fed chronic aflatoxin B1-contaminated diets.A total of 100 one-day-old male commercial line(Ross)broiler chickens were divided into 4 treatments,with 5 pens per treatment and 5 broiler chickens per *** were randomly assigned to 4 treatments,which were namely treatment 1(T1),control diet(CD);T2,CD+Sc at 1 g/kg;T3,CD+AFB1 at 100μg/kg;T4,CD+Sc at 1 g/kg+AFB1 at100μg/*** liver histopathology of broiler chickens fed diets with AFB1 showed diffused microvacuolar fatty *** addition of Sc showed normal hepatocytes similar to the *** small intestine villi from AFB1 group showed atrophy,hyperplasia of goblet cells,prominent inflammatory infiltrate and *** contrast,the small intestine villi from birds that received the yeast plus AFB1 showed an absence of inflammatory infiltrate,and atrophy;moreover,a lower number of goblet cells compared to the groups with AFB1 was *** morphometric intestine studies showed that a significant decrease(P0.05),there was a tendency to improve these *** residual levels of AFB1 in livers were significantly reduced(P<0.05)in the presence of the *** present work demonstrated that the addition of Sc alone or in combination with AFB1 in the broiler chicken diets had a beneficial effect in counteracting the toxic effects of AFB1 in livers besides improving the histomorphometric parameters and modulating the toxic effect of AFB1 in the intestine.

关键词： Aflatoxin B1 Broiler chickens Histomorphometry Histopathology toxic effects

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Helicobacter pylori and oral pathology:Relationship with the gastric infection

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World Journal of Gastroenterology 2014年第29期20卷 9922-9935页

作者： Isabel Adler andrea Muio Silvia Aguas Laura Harada Mariana Diaz Adriana Lence Mario Labbrozzi Juan Manuel Muio Boris Elsner alejandra Avagnina Valeria Denninghoff Oral Diseases Curriculum School of DentistryUniversity of Buenos AiresC1053ABJ Buenos AiresArgentina Department of Pathology Center for Medical Education and Clinical Research "Norberto Quirno" (CEMIC)1425 Buenos AiresArgentina National Scientific and Technical Research Council (CONICET) Career Member

Helicobacter pylori(***)has been found in the oral cavity and stomach,and its infection is one of the most frequent *** reviewed the literature and conducted a Topic Highlight,which identified studies reporting an association between ***-infection in the oral cavity and ***-positive stomach *** work was designed to determine whether *** is the etiologic agent in periodontal disease,recurrent aphthous stomatitis(RAS),squamous cell carcinoma,burning and *** selection focused on the highest quality studies and *** selected 48 articles reporting on the association between saliva and plaque and *** order to assess periodontal disease data,we included 12 clinical trials and 1 *** evaluated 13 published articles that addressed the potential association with RAS,and 6 with squamous cell *** publications focused on our questions on burning and *** is a close relation between *** infection in the oral cavity and the *** mouth is the first extra-gastric *** the role of *** in the etiology of squamous cell carcinoma,no evidence is still available.

关键词： Helicobacter pylori Oral pathology Gastric infection Burning and halitosis Recurrent aphthous stomatitis

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Robot-assisted oncologic pelvic surgery with Hugo™robot-assisted surgery system:A single-center experience

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Asian Journal of Urology 2023年第4期10卷 461-466页

作者： Angelo Territo Alessandro Uleri andrea Gallioli Josep Maria Gaya Paolo Verri Giuseppe Basile Alba Farré alejandra Bravo Alessandro Tedde Óscar Rodríguez Faba Joan Palou Alberto Breda Department of Urology FundacióPuigvertAutonomous University of BarcelonaBarcelonaSpain

Objective To report the outcomes of intra-and extra-peritoneal robot-assisted radical prostatectomy(RARP)and robot-assisted radical cystectomy(RARC)with Hugo™robot-assisted surgery(RAS)system(Medtronic,Minneapolis,MN,USA).Methods Data of twenty patients who underwent RARP and one RARC at our institution between February 2022 and January 2023 were *** primary endpoint of the study was to report the surgical setting of Hugo™RAS system to perform RARP and *** secondary endpoint was to assess the feasibility of RARP and RARC with this novel robotic platform and report the *** Seventeen patients underwent RARP with a transperitoneal approach,and three with an extraperitoneal approach;and one patient underwent RARC with intracorporeal ileal *** intraoperative complications *** docking and console time were 12(interquartile range[IQR]7-16)min and 185(IQR 177-192)min for transperitoneal RARP,15(IQR 12-17)min and 170(IQR 162-185)min for extraperitoneal *** intraoperative complications *** patient submitted to extraperitoneal RARP had a urinary tract infection in the postoperative period that required an antibiotic treatment(Clavien-Dindo Grade 2).In case of transperitoneal RARP,two minor complications occurred(one pelvic hematoma and one urinary tract infection;both Clavien-Dindo Grade 2).Conclusion Hugo™RAS system is a novel promising robotic platform that allows to perform major oncological pelvic *** showed the feasibility of RARP both intra-and extra-peritoneally and RARC with intracorporeal ileal conduit with this novel platform.

关键词： Bladder cancer Prostate cancer Radical cystectomy Radical prostatectomy Robotic surgery

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Validation of a preclinical dry eye model in New Zealand white rabbits during and following topical instillation of 1% ophthalmic atropine sulfate

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Animal Models and Experimental Medicine 2022年第3期5卷 266-273页

作者： alejandra Sánchez-Ríos Elba Yadira Correa-Gallegos José Manuel Medina-Espinoza andrea Anaid Navarro-Sanchez Oscar Olvera-Montaño Leopoldo Baiza-Durán Patricia Muñoz-Villegas Medical Affairs Department Laboratorios SophiaSA de CVZapopanJaliscoMexico

Background : The objective of this study was to validate an animal model for dry eye during and after the administration of 1% ophthalmic atropine sulfate(OAS) in New Zealand white(NZW) *** : OAS(1%) was applied three times per day to 30 eyes of 15 healthy NZW rabbits. Sacrifice, enucleation, and lacrimal gland removal took place on days 15, 21,and 30(OAS group). A second group(n = 5) was used as control. Clinical evaluations took place on days 3, 10, 15, 18, 21, 24 and 30. The primary endpoints were:Schirmer I test, tear break-up time(TBUT), and corneal fluorescein staining. As secondary endpoints, clinical changes including intraocular pressure, and histopathology were *** : While OAS was administered, the Schirmer I test showed a statistically significant reduction for OAS group versus control( p < 0.001), and versus basal production( p < 0.001). TBUT showed statistically significant differences between groups(days 3 and 10;p = 0.001) and versus basal values(day 3;p < 0.001). Fluorescein staining showed a statistically significant difference(day 3;p = 0.001). The most frequent clinical finding was conjunctival hyperemia(76.9% OAS vs. 20% control). For histopathology, all OAS subjects presented some degree of inflammation(86.7% minimal;13.3% mild) whereas the control presented only 30% minimal inflammation. Goblet cell density showed no *** : The effectiveness of the OAS dry eye model in NZW rabbits as reported in previous studies was confirmed, provided that the application of the drug is maintained throughout the intervention;it is not a viable model after OAS administration is suspended.

关键词： atropine sulfate dry eye rabbit model safety Schirmer I test TBUT

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Detection of Antinuclear Antibodies in Canine Serum by Immunoperoxidase in MDCK and Indirect Immunofluorescence in HEp2 Cells

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Open Journal of Veterinary Medicine 2023年第5期13卷 41-52页

作者： Guillermo Valdivia Anda Edgar Guillermo Valdivia Lara Ana Cristina Calderón Uribe alejandra andrea Escobar Patiño Gloria Elena Lara Reyes Laboratorio de Patogenicidad Microbiana Unidad de Investigación Multidisciplinaria FESC UNAM México City México Departamento de Patología Universidad Complutense de Madrid Madrid Espa&#241a Laboratorio DIVeT^® </sup> Estado de México México City México

Antinuclear antibodies are found in animals suffering from Systemic Lupus Erythematosus (SLE) and some other diseases, their presence in the blood is determined by antinuclear antibody (ANA) test using indirect immunofluorescence (IF) with HEp2 cells as a substrate. In this work, an immunoperoxidase (IP) assay was developed to evaluate the ANAs in canine sera, using canine kidney cell lines (MDCK) and compared with a commercial immunofluorescence test on Hep2 cells for this system, a fluoresceinated anti-canine Ig antibody was standardized. The study was performed on 50 sera from dogs submitted to the laboratory with different clinical diagnoses of autoimmune-associated diseases. The procedures on both cells were unified to perform comparisons of the reactions, direct sera or at different dilutions were added to a monolayer of permeabilized MDCK cells, followed by a peroxidized anti-canine IgG conjugate, and a substrate for the IP reaction. The same sera were tested on the commercial IF assay on Hep2 cell system. In 22/50 cases, the presence of LE cells in peripheral blood was determined. A high correlation was found in the detection of antinuclear antibodies between both cell lines and techniques, however there were differences in the reaction patterns in the nucleus and cytoplasm between cell lines. The diffuse nuclear pattern observed in MDCK cells was more related to the presence of high percentages of LE cells in peripheral blood. The differences found in the results were possibly associated with the presence of homologous antigens between the MDCK cells and the dog. In addition, the methodology and standardization for the use and interpretation of a reference serum was developed to unify the interpretation criteria in the laboratory.

关键词： Antinuclear Antibodies Immunoperoxidase in Marbin Darbin Canine Kidney (MDCK) SLE Associate Disease

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产前应激及炎症相关基因的甲基化水平对儿童早期肥胖指标影响的队...

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2017环境与公共健康学术会议暨中国环境科学学会环境医学与健康分会、中国毒理学会生化与分子毒理专业委员会2017年年会

作者： Shaowei Wu Chris Gennings Rosalind J.Wright ander Wilson Heather H.Burris Allan C.Just Joseph M.Braun Katherine Svensson Jia Zhong Kasey J.M.Brennan Alexandra Dereix alejandra Cantoral Lourdes Schnaas Martha Maria Tellez-Rojo Robert O.Wright andrea A.Baccarelli Department of Occupational and Environmental Health Sciences School of Public HealthPeking University Population Health and Science Policy Mount Sinai Hospital The Mindich Child Health & Development Institute Icahn School of Medicine at Mount Sinai Department of Statistics Colorado State University Department of Neonatology and Obstetrics Gynecology and Reproductive BiologyBeth Israel Deaconess Medical CenterHarvard Medical School Department of Preventive Medicine Icahn School of Medicine at Mount Sinai Department of Epidemiology School of Public HealthBrown University Department of Environmental Health Sciences Mailman School of Public HealthColumbia University

Background:Maternal stress during pregnancy may influence childhood growth and adiposity,possibly through immune/inflammatory *** investigated whether exposure to prenatal stress and methylation in inflammation-related genes were associated with childhood adiposity in424 mother-child pairs in Mexico City,***:A stress index was created based on four

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