Characterization of oral yeasts isolated from healthy individuals attended in different Colombian dental clinics

作     者：Raul Eduardo Rivera Alejandra Zuluaga Karen Arango Itzjak Kadar Paola Andrea Pinillos Luis Fernando Montes Eugenia Catalina Cepeda Ernesto Gonzalez Pedro Antonio Alfonso Andrea Alejandra Villalba Luis Fernando Casanova Adolfo Perez Armando Roa Martha Jhoana Arias Jorge Orlando Francisco Cuellar Lorena Pedraza Adiel Alberto Vasquez Blanca Lynne Suarez Beatriz LGomez Catalina De Bedout Luz Elena Cano 

作者机构：Group of Investigation in Oral HealthFaculty of DentistryAntonio Narino UniversityArmeniaQuindio 630001Colombia Medical and Experimental MycologyUnitCorporation for Biological Research(CIB)MedellinAntioquia 050034Colombia School of Medicine and Health SciencesUniversidad del RosarioCarreraBogota 111221Colombia School of MicrobiologyUniversity of AntioquiaMedellinAntioquia 050036Colombia. 

出 版 物：《The Journal of Biomedical Research》 (生物医学研究杂志（英文版）)

年 卷 期：2019年第33卷第5期

页      面：333-342页

核心收录：

学科分类：0710[理学-生物学] 1003[医学-口腔医学] 10[医学] 

基　　金：supported by the Antonio Narino University in collaboration with the Biological Research Corporation(CIB)and following research:Angela Restrepo Moreno,from CIB,for scientific support and advice Maria Dolly Garcia,from Research Group and Counseling in Statistics of the University of Quindío,statistical support Jorge Enrique Gómez,Nestor Ivan Cardona,and Aylan Farid Arenas,from Group GEPAMOL,Center for Biomedical Research,University of Quindío,for providing language help and writing assistance 

主　　题：oral yeast Candida species microbial epidemiology 

摘      要：The aim of this study was to identify the most frequent yeasts in the oral cavity of adult individuals without immune disorders and to associate the presence of these oral yeasts with different characteristics of each individual. Oral rinse samples were obtained from 96 healthy adults and cultured in Sabouraud dextrose agar media and CHROMagar. Yeasts were identified by sequencing the D1/D2 region of the 28 S rRNA gene. Probable association among the socio-demographic characteristics, body mass index, family and personal medical history,oral hygiene, tobacco and/or alcohol consumption habits and presence of oral fungi was analyzed. Contingency tables and logistic regression were employed to evaluate possible relationships between the presence of oral fungi and mixed colonization with these variables. 57.3% of the healthy individuals had oral yeasts and 21.8% had mixed colonization. The most prevalent yeasts were Candida albicans(52%), C. parapsilosis(17.9%), and C.dubliniensis(7.57%). Yeasts with most frequently mixed colonization were C. albicans and C. parapsilosis. No relationships were found among the variables analyzed. However, the presence of mixed colonization was related to the presence of dental prostheses(P0.006), dental apparatuses(P=0.016) and O’Leary index(P=0.012). This is the first study that characterized oral yeasts in Colombian healthy individuals, determined the most prevalent oral yeasts C. albicans, C. parapsilosis and C. dublinensis and an association of mixed colonization with the use of dental prostheses and aparatology and poor hygiene.