检索结果-南通市图书馆

Cannabinoid Modulation of Monoamine Levels in Mouse Brain:Unveiling Neurochemical Dynamics through an innovative High-performance Liquid Chromatography-Fluorescence Detection Bioanalysis

引用

Journal of analysis and Testing 2024年第3期8卷 300-314页

作者： Fonseca Carla Cunha Susana Ferreira Ricardo Lapo pais Mariana Gon?alves Joana Camins Antoni Ettcheto Miren Barbosa Rui Fortuna ana Laboratory of Pharmacology Faculty of Pharmacy University of Coimbra CiBiT/iCNAS-Coimbra institute for Biomedical imaging and Translational Research University of Coimbra Department of Pharmacology Toxicology and Therapeutic Chemistry Faculty of Pharmacy and Food ScienceUniversitat de Barcelona institute of Neurosciences Universitat de Barcelona CiBiT/iCNAS-Coimbra institute for Biomedical imaging and Translational Research University of Coimbra Faculty of Sciences and Technology Doctoral Program in Biomedical Engineering University of Coimbra Biomedical Research Networking Centre in Neurodegenerative Diseases (CiBERNED) Carlos Ⅲ Health Institute institut d’investigació Sanitària Pere Virgili (iiSPV) CNC-Center for Neuroscience and Cell Biology University of Coimbra

Homeostasis of monoaminergic pathways is compromised in aging and neurodegenerative/neurological diseases such as Alzheimer's disease and depression. On the other hand, their modulation has also been linked to the mechanism of action of several drugs. Therefore, monitoring the levels of noradrenaline(NA), adrenaline(AD), dopamine(DA), and serotonin(5-HT), as well as those of tryptophan(TRYP), the precursor of 5-HT, and DA metabolites, namely 3,4-dihydroxyphenylaceticacid(DOPAC) and homovanillicacid(HVA), is fundamental for assessing disease severity and progression. This work aimed to develop and validate the first High-Performance Liquid Chromatography(HPLC) coupled with fluorescence detector(FLD) method that simultaneously and accurately quantifies NA, AD, DA, DOPAC, HVA, 5-HT and TRYP in mouse brain and prefrontal cortex(PFC) matrices. Previous sample preparation by protein precipitation was required to extract the compounds. Calibration curves were plotted using the background subtraction approach to reduce the interference of the endogenous analytes. intra and inter-day accuracy and precision were within the ranges defined by iCH(The international Council for Harmonisation) guideline for bioanalytical method validation. Following validation, the impact of cannabidiol(CBD), cannabigerol(CBG), and cannabidivarin(CBDV) was explored in mice brains post-administration,revealing significant alterations in specific neurotransmitter levels upon cannabinoid exposure and shedding light on the complex modulation of neurochemical dynamics by cannabinoids. This research highlights the fit-for-purpose of the HPLC-FLD method and provides insights into potential mechanisms underlying phytocannabinoid actions in the central nervous system(CNS).

关键词： Brain Cannabinoid HPLC Mice Neurotransmitter Prefrontal cortex

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Mountain invasions on the way:are there climatic constraints for the expansion of alien woody species along an elevation gradient in Argentina?

引用

Journal of Plant Ecology 2016年第4期9卷 380-392页

作者： Paula A.Tecco ana i.pais-bosc. Guillermo Funes Paula i.Marcora Sebastián R.Zeballos Marcelo Cabido Carlos Urcelay instituto Multidisciplinario de BiologíaVegetal(CONiCET-UNC) Velez Sarsfield 1611.Córdoba 5000Argentina Facultad de Ciencias Exactas Físicas y Naturales Universidad Nacional de CórdobaVelez Sarsfield 299Córdoba 5000Argentina Facultad de Ciencias Agrarias Universidad Nacional de Catamarca.Maestro Quiroga 51Catamarca 4700Argentina

Aims increasing evidence worldwide indicates that high mountain regions are not immune to invasion by alien ***,we aimed to address whether three major woody invaders of low-mountain areas are constrained to lower altitudes due to climatic restrictions or just by low propagule *** hypothesize that the increasing climatic harshness towards higher altitudes restricts seedling establishment and survival of these woody invaders and thus their potential for altitudinal *** The study was carried out in the Sierras Grandes Mountains,Córdoba,central Argentina(32°50′S,64°90′W).We addressed the hypothesis with an experimental approach,dissociating the effect of the environmental gradient from that of propagule pressure,by discarding the influence of seed *** translocated seeds of Gleditsia triacanthos L.,Ligustrum lucidum *** Pyracantha angustifolia(Franch.)*** the altitudinal gradient(from 900 to 2700 m asl).Seven sites were selected along the altitudinal gradient,spaced every 200-400 *** plots(4×4 m)were selected within each altitudinal site and excluded from livestock;100 seeds of each species were sown within each plot(6300 seeds in total).Seedling emergence and survival was monitored during two growing seasons and soil temperature and moisture was recorded as *** field experiment was complemented with lab *** Findings Climate along this altitudinal gradient might be least restrictive at mid elevations,as a result of the intersection between opposite soil temperature and moisture *** seeds germinated along the whole altitudinal gradient and seedlings successfully established and over-wintered well above their current altitudinal distribution(up to 2200 m for Ligustrum and 2400 m for Gleditsia and Pyracantha).Additional lab assays confirmed field patterns and gave some insights into contrasting regeneration strategies between these invaders that might help to overcome stochastic e

关键词： altitudinal gradient exotic species Gleditsia triacanthos Ligustrum lucidum Pyracantha angustifolia seedling establishment

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A Stochastic Variational Approach to Viscous Burgers Equations

引用

Acta Mathematica Sinica,English Series 2016年第9期32卷 1027-1034页

作者： ana Bela CRUZEiRO Guo Ping LiU GFMUL and Dep. de Matematica instituto Superior Tecnico (Universidade de Lisboa) Av. Rovisco Pais 1049-001 Lisboa Portugal GFMUL and Dep. de Matematica instituto Superior Tecnico (Universidade de Lisboa) Av. Rovisco Pais 1049-001 Lisboa Portugal Academy of Mathematics and Systems Science Chinese Academy of Sciences 100190 Beijing P. R. China

We consider viscous Burgers equations in one dimension of space and derive their solutions from stochastic variational principles on the corresponding group of homeomorphisms. The metrics considered on this group are Lp metrics. The velocity corresponds to the drift of some stochastic Lagrangian processes. Existence of minima is proved in some cases by direct methods. We also give a representation of the solutions of viscous Burgers equations in terms of stochastic forward-backward systems.

关键词： Burgers equations stochastic variational principles Lagrangian diffusions

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

L^p-gradient Estimates of Symmetric Markov Semigroups for 1 〈 p ≤ 2

引用

Acta Mathematica Sinica,English Series 2006年第1期22卷 101-104页

作者： ana Bela CRUZEiRO Xi Cheng ZHANG Department Matemdtica I.S.T. Av. Rovisco Pais 1099-01 Lisboa Portugal and Grupo de Fisica-Matemdtica U.L. Lisboa Portugal Department of Mathematics Huazhong University of Science and Technology Wuahan 430074 P. R. China

For 1 〈 p ≤2, an L^p-gradient estimate for a symmetric Markov semigroup is derived in a general framework, i.e. ‖Γ^/2（Ttf）‖p≤Cp/√t‖p, where F is a carre du champ operator. As a simple application we prove that F1/2（（i- L） ^-α） is a bounded operator from L^p to L^v provided that 1 〈 p 〈 2 and 1/2〈α〈1. For any 1 〈 p 〈 2, q 〉 2 and 1/2 〈α 〈 1, there exist two positive constants cq,α,Cp,α such that ‖Df‖p≤ Cp,α‖（i - L）^αf‖p,Cq,α（i-L）^（1-α）‖Df‖q＋‖f‖q, where D is the Malliavin gradient （[2]） and L the Ornstein-Uhlenbeck operator.

关键词： Symmetric Markov semigroup Carre du champ Path space

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Preliminary Developments of Miniplate-Type Fuel of U-2.5Zr-7.5Nb Alloy Dispersed and Cladded in Zircaloy

引用

Journal of Energy and Power Engineering 2014年第8期8卷 1409-1414页

作者： Natalia Mattar Cantagalli Kelly Cristina Martins Faeda Rafael Witter Dias pais ana Maria Matildes dos Santos Wilmar Barbosa Ferraz CDTN/CNEN (Centro de Desenvolvimento da Tecnologia Nuclear) Belo Horizonte 31270-901 Brazil

Nuclear fuel based on uranium metal alloys is utilized in research and test reactors. For the purpose of the reduction of fuel enrichment, high densities of uranium-235 in this kind of fuel are needed. This can be achieved when uranium alloys are used containing elements such as Zr, Mo and Nb. The construction of fuel element with high-uranium density requires materials with low cross sections for neutron absorption, stability under irradiation and absence of the chemical interactions between the fuel and cladding elements. in case of U-Zr-Nb alloys, Zry （zircaloy） cladding is a better option due to the fact that they have a higher chemical compatibility when compared with the use of aluminum alloys. This study aims to develop plate type nuclear fuel using the U-2.5Zr-7.5Nb alloy dispersed in Zry. Powders of this uranium based alloy and Zry were obtained by hydriding-dehydriding process. These powders were homogenized, compacted in pellet that was sandwiched in plates and frame of Zry. This assembly was hot rolled forming the dispersion fuel miniplate.

关键词： Nuclear fuel plate U-Zr-Nb alloy Zry

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Search for desisn intellisence： A field study on the role of emotional intellisence in architectural design studios

引用

Frontiers of Architectural Research 2014年第4期3卷 413-423页

作者： Sajjad Nazidizaji ana Tome Francisco Resateiro iCiST DECivil Instituto Superior Tecnico Universidade de Lisboo Avenida Rovisco Pais 1Lisboa 1049-001 Portugal CESUR DECivil Instituto Superior Tecnico Universidade de Lisboa Avenida Rovisco Pais ILisboa 1049-001 Portugal

The design studio is the core of the architecture curriculum. interpersonat interactions have a key rote during the processes of design and critique. The influence of emotional intelligence （EQ） on interpersonal communication skirts has been widely proven. This study examines the correlation between EQ and architecturat design competence, To achieve this, 78 architecture students were setected via a simple random sampling method and tested using an EO. test questionnaire developed by Bradbury and Greaves （2006）. The scores of five architectural design studio courses （ADS-1, ADS-2, ADS-3, ADS-4, and ADS-5） were used as indicators of the progress in design of the students. Descriptive and inferential statistics methods were both employed to anatyze the research data. The methods inctuded corretation analysis, mean comparison t-test for independent samptes, and single sample t-test. Findings showed no significant relationship between EQ and any of the indicators.

关键词： Emotional intelligence (EQ) Architectural education Design competence Design studio

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Characterization of the Topography and Wettability of English Weed Leaves and Biomimetic Replicas

引用

Journal of Bionic Engineering 2014年第3期11卷 346-359页

作者： Pedro Miguel Matos Pereira ana Sofia Moita Gabriel Amaro Monteiro Duarte Miguel Franca Prazeres iBB - institute for Biotechnology and Bioengineering Centre for Biological and Chemical Engineering Department of Bioengineering Instituto Superior Tecnico Universidade de Lisboa Av. Rovisco Pals 1049-001 Lisboa Portugal iN+ - Center for innovation Technology and Policy Research Instituto Superior T~cnico Universidade de Lisboa Av. Rovisco Pais 1049-001 Lisboa Portugal

The topography and wettability of the underside of English weed （Oxalis pes-caprae） leaves and of their biomimetic replicas are investigated. Polyvinyl siloxane molds were cast from the leaves and then filled with an epoxy pre-polymer to produce replicas. The particular topographical structures of leaves and replicas were evaluated by optical microscopy and Scanning Electron Microscopy （SEM） analysis. The static wettability of leaves and replicas was assessed by contact angle measurements, while the dynamic wettability was characterized by estimating contact angle hysteresis and studying the dynamic behavior of impacting water droplets. A smooth glass slip and its replica were used as control surfaces. The replica moulding method used was able to transfer the characteristic pattern of irregular 100 μm - 200 μm × 60 μm convex papillae interspersed with stomata of the original leaf to the epoxy replicas. The static contact angle of 143°± 3° and the contact angle hysteresis of 2~ indicate that the underside of the English weed leaf is close to superhydrophobic. The lower contact angles （130° ± 4°） and higher hysteresis （31°） observed for the replica when compared with the original leaves were associated to an inaccurate replication of the chemistry and structures of the three-dimensional wax projections covering the plant surface. Also, trichomes in the original leaves could not be accurately reproduced due to their flexibility and fragility. Differences in wetting behavior were also evident from droplet impact experiments, with rebound regimes prevailing in the original leaves and regimes characterized by higher adhesion and larger dissipation predominating in the replicas. Nevertheless, the morphological features of the leaf transferred to the replica were sufficient to promote a clear hydrophobic behavior of the replica when compared with the smooth epoxy reference surface.

关键词： biomimetic surfaces oxalis pes-caprae English weed replica molding wettability micro-topography

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Dynamics of Droplets of Biological Fluids on Smooth Superhydrophobic Surfaces under Electrostatic Actuation

引用

Journal of Bionic Engineering 2016年第2期13卷 220-234页

作者： ana Sofia Moita Catarina Laurencia Joana anastacio Ramos Duarte Miguel Franca Prazeres Antonio Luis Nobre Moreira iN+ - Center for innovation Technology and Policy Research Instituto Superior Tecnico Universidade de Lisboa Av. Rovisco Pais 1049-001 Lisboa Portugal iBB - institute for Bioengineering and Biosciences Department of Bioengineering Instituto Superior Tecnico Universidade de Lisboa Av. Rovisco Pais 1049-001 Lisboa Portugal

This study describes the dynamic behaviour of droplets of biological liquids on hydrophobic surfaces under electrostatic actuation, to devise sample handling in lab-on-chip diagnostic tools. Bovine Serum Albumin （BSA） is taken as a representative biomolecule, since it is often used in adsorption studies. Green Fluorescence Protein （GFP） is also considered, given its natural fluorescence. Several effects such as sample concentration and pH are discussed. The results show negligible effects of proteins concentration in electrowetting, although increased concentrations endorse passive adsorption mechanisms, which alter the local wettability of the substrates precluding droplet motion. Bioinspired surfaces promote the largest spreading diameter, which is beneficial for droplet motion. However, surface roughness promotes energy dissipation limiting the receding droplet motion. Hence, the most effective approach is altering the surface chemistry. The coating is applied to a surface with a mean roughness smaller than 20 nm and does not alter significantly the topography, thus leading to the so-called smooth superhydrophobic surface. This coating also reduces passive proteins adsorption, as confirmed by Confocal Microscopy （CM）, which is beneficial for droplet motion. Evaluating absorption spectra of protein solutions evidences an increase in protein concentration ascribed to droplet evaporation as confirmed by theoretical analysis and time resolved infrared visualization.

关键词： bioinspired surfaces biofluids electrostatic actuation hydrophobicity adsorption evaporation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Application of Bioinspired Superhydrophobic Surfaces in Two-phase Heat Transfer Experiments

引用

Journal of Bionic Engineering 2017年第3期14卷 506-519页

作者： Emanuele Teodori ana Sofia Moita Miguel Moura Pedro Pontes Antonio Moreira Yuan Bai Xinlin Li Yan Liu iN+- Center for innovation Technology and Policy Research Instituto Superior T~enico Universidade de Lisboa Av. Rovisco Pais 1049-001 Lisboa Portuga Key Laboratory of Bionic Engineering (Ministry of Education) Jilin University Changchun 130022 China

This paper addresses the potential to use Lotus leafbioinspired surfaces in applications involving heat transfer with phase change, namely pool boiling and spray impingement. Besides describing the role of bioinspired topographical features, using an innovative technique combining high-speed visualization and time-resolved infrared thermography, surface durability is also addressed. Water is used for pool boiling and for spray impingement systems （simplified as single droplet impact）, while HFE7000 is used in a pool boiling cooler for electronic components. Results show that surface durability is quickly compro- mised for water pool boiling applications, as the chemical treatment does not withstand high temperatures （T 〉 100 ℃） during long time intervals （3 h - 4 h）. For HFE7000 pool boiling （depicting lower saturation temperature - 34 ℃）, heat transfer en- hancement is governed by the topography. The regular hierarchical pattern of the bioinspired surfaces promotes the heat transfer coefficient to increase up to 22.2%, when compared to smooth surfaces, while allowing good control of the interaction mechanisms until a distance between micro-structures of 300 gm- 400 ixm. Droplet impingement was studied for surface temperatures ranging between 60 ℃ - 100 ℃. The results do not support the use of superhydrophobic surfaces for cooling applications, but reveal great potential for other applications involving droplet impact on heated surfaces （e.g. metallurgy in- dustry）.

关键词： bioinspired surfaces surface micro-patterning two-phase heat transfer time resolved infrared thermography

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bubble Dynamics and Heat Transfer on Biphilic Surfaces:Experiments and Numerical Simulation

引用

Journal of Bionic Engineering 2020年第4期17卷 809-821页

作者： Pedro Pontes Ricardo Cautela Emanuele Teodori ana Sofia Moita anastasios Georgoulas António Luís Nobre Moreira Moreira iN+-Center for innovation Technology and Policy ResearchDepartment of Mechanical EngineeringInstituto Superior TécnicoUniversidade de LisboaAv.Rovisco Pais1049-001 LisboaPortugal ASML Holding N.V. De Run 65015504 DRVeldhovenThe Netherlands Department of Exact Sciences and Engineering Portuguese Military AcademyR.Gomes Freire2031169-203 LisboaPortugal Advanced Engineering Centre School of ComputingEngineering and MathematicsCockcroft BuildingLewes RoadUniversity of Brighton BN24GJUK

Wettability is known to play a major role in enhancing pool boiling heat *** this context bioinspired surfaces can bring significant advantages in pool boiling *** work addresses a numerical investigation of bubble growth and detachment on a biphilic surface pattern,namely in a superhydrophobic region surrounded by a hydrophilic *** characteristics resemble bioinspired solutions explored in our research group,mainly considering the main topographical *** numerical approach is intended to provide additional information to an experimental approach,allowing to obtain temperature,pressure and velocity fields in and around the bubble,which help to describe bubble *** model was validated based on experimental data obtained with extensive image processing of synchronized high-speed video and high-speed thermographic *** results obtained here clearly evidence that combining enhanced direct numerical simulations with high-resolution transient experimental measurements is a promising tool to describe the complex and intricate hydrodynamic and heat transfer phenomena governing pool boiling on heated biphilic surfaces.

关键词： bioinspired biphilic surfaces bubble dynamics two-phase heat transfer time resolved infrared thermography CFD model

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：