Cannabinoid Modulation of Monoamine Levels in Mouse Brain:Unveiling Neurochemical Dynamics through an Innovative High‑performance Liquid Chromatography‑Fluorescence Detection Bioanalysis

作     者：Fonseca Carla Cunha Susana Ferreira Ricardo Lapo Pais Mariana Goncalves Joana Camins Antoni Ettcheto Miren Barbosa Rui Fortuna Ana Fonseca Carla;Cunha Susana;Ferreira Ricardo;Lapo Pais Mariana;Gonçalves Joana;Camins Antoni;Ettcheto Miren;Barbosa Rui;Fortuna Ana

作者机构：Laboratory of PharmacologyFaculty of PharmacyUniversity of CoimbraPolo das Ciencias da SaudeAzinhaga de Santa CombaCoimbra 3000‑548Portugal CIBIT/ICNAS-Coimbra Institute for Biomedical Imaging and Translational ResearchUniversity of CoimbraCoimbra 3000‑548Portugal Department of PharmacologyToxicology and Therapeutic ChemistryFaculty of Pharmacy and Food ScienceUniversitat de BarcelonaBarcelona 08028Spain Institute of NeurosciencesUniversitat de BarcelonaBarcelona 08035Spain Faculty of Sciences and TechnologyDoctoral Program in Biomedical EngineeringUniversity of CoimbraCoimbra 3004‑516Portugal Biomedical Research Networking Centre in Neurodegenerative Diseases(CIBERNED)Carlos Ⅲ Health InstituteMadrid 28031Spain Institut d’Investigacio Sanitaria Pere Virgili(IISPV)Reus 43204Spain CNC-Center for Neuroscience and Cell BiologyUniversity of CoimbraCoimbra 3004-504Portugal 

出 版 物：《Journal of Analysis and Testing》 (分析检测（英文）)

年 卷 期：2024年第8卷第3期

页      面：300-314页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 0703[理学-化学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by funds from Fundacao para a Ciencia e a Tecnologia(FCT),Portugal,European Union,MCTES,and Fundo Social Europeu(FSE)in the scope of the Centro 2020 Regional Operational Programme(Carla’s PhD research grant 2021.06135 BD and Mariana’s PhD research grant 2020.6582.BD) supported by FCT Exploratory Project 2022.01066.PTDC,COMPETE and FEDER funds,FCT,Portugal.Figures 1 and 2 were created with BioRender.com 

主　　题：Neurotransmitter HPLC Mice Brain Prefrontal cortex Cannabinoid 

摘      要：Homeostasis of monoaminergic pathways is compromised in aging and neurodegenerative/neurological diseases such as Alzheimer’s disease and *** the other hand,their modulation has also been linked to the mechanism of action of several ***,monitoring the levels of noradrenaline(NA),adrenaline(AD),dopamine(DA),and serotonin(5-HT),as well as those of tryptophan(TRYP),the precursor of 5-HT,and DA metabolites,namely 3,4-dihydroxyphenylacetic acid(DOPAC)and homovanillic acid(HVA),is fundamental for assessing disease severity and *** work aimed to develop and validate the first High-Performance Liquid Chromatography(HPLC)coupled with fluorescence detector(FLD)method that simultaneously and accurately quantifies NA,AD,DA,DOPAC,HVA,5-HT and TRYP in mouse brain and prefrontal cortex(PFC)*** sample preparation by protein precipitation was required to extract the *** curves were plotted using the background subtraction approach to reduce the interference of the endogenous *** and inter-day accuracy and precision were within the ranges defined by ICH(The International Council for Harmonisation)guideline for bioanalytical method *** validation,the impact of cannabidiol(CBD),cannabigerol(CBG),and cannabidivarin(CBDV)was explored in mice brains post-administration,revealing significant alterations in specific neurotransmitter levels upon cannabinoid exposure and shedding light on the complex modulation of neurochemical dynamics by *** research highlights the fit-for-purpose of the HPLC-FLD method and provides insights into potential mechanisms underlying phytocannabinoid actions in the central nervous system(CNS).