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Constructing a biofunctionalized 3D-printed gelatin/sodium alginate/chitosan tri-polymer complex scaffold with improvised biological andmechanical properties for bone-tissue engineering

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Bio-Design and Manufacturing 2024年第1期7卷 57-73页

作者： amit kumar singh Krishna Pramanik amit Biswas Centre of Excellence in Tissue Engineering Department of Biotechnology and Medical EngineeringNational Institute of TechnologyRourkela 769008India

Sodium alginate(SA)/chitosan(CH)polyelectrolyte scaffold is a suitable substrate for tissue-engineering *** present study deals with further improvement in the tensile strength and biological properties of this type of scaffold to make it a potential template for bone-tissue *** experimented with adding 0%–15%(volume fraction)gelatin(GE),a protein-based biopolymer known to promote cell adhesion,proliferation,and *** resulting tri-polymer complex was used as bioink to fabricate SA/CH/GEmatrices by three-dimensional(3D)*** studies using scanning electron microscopy revealed the microfibrous porous architecture of all the structures,which had a pore size range of 383–419μm.X-ray diffraction and Fourier-transform infrared spectroscopy analyses revealed the amorphous nature of the scaffold and the strong electrostatic interactions among the functional groups of the polymers,thereby forming polyelectrolyte complexes which were found to improve mechanical properties and structural *** scaffolds exhibited a desirable degradation rate,controlled swelling,and hydrophilic characteristics which are favorable for bone-tissue *** tensile strength improved from(386±15)to(693±15)kPa due to the increased stiffness of SA/CH scaffolds upon addition of *** enhanced protein adsorption and in vitro bioactivity(forming an apatite layer)confirmed the ability of the SA/CH/GE scaffold to offer higher cellular adhesion and a bone-like environment to cells during the process of tissue *** vitro biological evaluation including the MTT assay,confocal microscopy analysis,and alizarin red S assay showed a significant increase in cell attachment,cell viability,and cell proliferation,which further improved biomineralization over the scaffold *** addition,SA/CH containing 15%gelatin designated as SA/CH/GE15 showed superior performance to the other fabricated 3D structures,demonstrating its pote

关键词： Scaffold Biomaterial Sodium alginate Chitosan Gelatin 3D printing Tissue engineering

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CADSE： communication aware design space exploration for efficient run-time MPSoC management

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Frontiers of Computer Science 2013年第3期7卷 416-430页

作者： amit kumar singh Akash kumar Jigang WU Thambipillai SRIKANTHAN Department of Electrical and Computer Engineering National University of Singapore Singapore 119077 Singapore School of Computer Science and Software Engineering Tianjin Polytechnic University Tianjin 300160 China School of Computer Engineering Nanyang Technological University Singapore 639798 Singapore

Real-time multi-media applications are increasingly mapped on modern embedded systems based on multiprocessor systems-on-chip （MPSoC）. Tasks of the applications need to be mapped on the MPSoC resources efficiently in order to satisity their performance constraints. Exploring all the possible mappings, i.e., tasks to resources combinations exhaustively may take days or weeks. Additionally, the exploration is performed at design-time, which cannot handle dynamism in applications and resources＇ status. A runtime mapping technique can cater for the dynamism but cannot guarantee for strict timing deadlines due to large computations involved at run-time. Thus, an approach performing feasible compute intensive exploration at design-time and using the explored results at run-time is required. This paper presents a solution in the same direction. Communicationaware design space exploration （CADSE） techniques have been proposed to explore different mapping options to be selected at run-time subject to desired performance and available MPSoC resources. Experiments show that the proposed techniques for exploration are faster over an exhaustive exploration and provides almost the same quality of results.

关键词： multiprocessor systems-on-chip design space exploration run-time mapping synchronous dataflow graphs throughput

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Evaluating Fertilization Effects on Soil Physical Properties Using a Soil Quality Index in an Intensive Rice-Wheat Cropping System

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Pedosphere 2016年第6期26卷 887-894页

作者： Bappa DAS Debashis CHAKRABORTY Vinod kumar singh Mukhtar AHMED amit kumar singh Arijit BARMAN Division of Agricultural Physics Indian Agricultural Research InstituteNew Delhi 110012(India) Project Directorate for Farming Systems Research ModipuramMeerut 250110(India) Division of Soil Science and Agricultural Chemistry Indian Agricultural Research InstituteNew Delhi 110012(India)

Soil quality assessment has been suggested as an effective tool for evaluating sustainability of soil and crop management *** objective of this study was to develop a sensitive soil quality index(SQI) based on bulk density(BD),water-holding capacity(WHC),water-stable aggregates(WSA),aggregate mean weight diameter(AMWD),total organic C(TOC) and C input to evaluate the important rice-wheat cropping system on an Inceptisol in India.A long-term experiment has been conducted for 18 years at the Indian Council of Agricultural Research-Indian Institute of Farming Systems Research,Modipuram,*** treatments selected for this study were comprised of a no-fertilizer control and N,P and K fertilizers(NPK) combined with Zn and S fertilizers(NPK+ Zn+S),farmyard manure(NPK+FYM),green gram residues(NPK+GR) and cereal residues(NPK+CR),laid out in a randomized complete block design with three *** samples were collected and analyzed for BD,WHC,WSA and *** analysis revealed that both rice and wheat yields signi?cantly increased with the increases in AMWD,TOC and C input,but decreased with the increase in *** SQI values were then generated based on regression analysis of BD,WSA,AMWD,TOC and C input with rice and wheat yields for the 0–15 and 15–30 cm soil layers,*** analyses between crop yields and SQI values showed a quadratic type of relation with the coeffcient of determination(R^2) varying from 0.78 to *** regard to soil sustainability,applying crop residues to both rice and wheat could maintain soil quality for a longer period,whereas the highest yields of both the crops were recorded in the NPK+Zn+S *** regression equations developed in this study could be used to monitor soil quality in a subhumid tropical rice-wheat cropping system.

关键词： bulk density C input integrated nutrient management total organic C water-holding capacity water-stable aggregates

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Effects of in ovo delivered xylo-and mannan-oligosaccharides on growth performance,intestinal immunity,cecal short-chain fatty acids,and cecal microbiota of broilers

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Journal of Animal Science and Biotechnology 2022年第4期13卷 1099-1114页

作者： amit kumar singh Utsav Prakash Tiwari Birendra Mishra Rajesh Jha Department of Human Nutrition Food and Animal SciencesUniversity of Hawaii at Manoa1955 East-West RdHonoluluHI 96822USA

Background:This study investigated a novel in ovo feeding strategy to determine the prebiotic effects of xylo-and mannan-oligosaccharides(XOS and MOS)differing in the degree of polymerization.A total of 192 fertilized eggs were divided into 6 treatment groups:i)normal saline control(NSC),ii)xylotriose(XOS3),iii)xylotetraose(XOS4),iv)mannotriose(MOS3),v)mannotetraose(MOS4),and vi)no injection control(NIC),each containing 4 replicate trays with 8 eggs per *** d 17 of incubation,3 mg of oligosaccharides(except for controls)dissolved in 0.5 mL of 0.85%normal saline were injected into the amnion of Cobb 500 broilers *** hatch,the chicks were raised for 28 d under standard husbandry practices and were fed a commercial broilers diet ad libitum,and samples were collected ***:The hatchability,growth performance,and relative weights of breast,drumstick,liver,and proventriculus were not different among the treatments(P>0.05).The XOS3 injection increased the total short-chain fatty acid production at d 28 compared with both control groups(P0.05).On the hatch day,the expression level of the CD3 gene(a T cell marker)was increased by XOS3,while the IL-10 gene(a marker of anti-inflammatory cytokine)was reduced by MOS4(P0.05),except XOS4 increased IL-4 compared with NSC(P<0.05).The broilers in the MOS4 group had higher operational taxonomic units(OTUs)and had more differentially abundant taxa,including order Lactobacillales and family Leuconostocaceae(P<0.05)than both controls on d *** predictive functional profiling indicated that the linoleic acid metabolism pathway w

关键词： Broilers Gut health Immunity in ovo Mannanoligosaccharides Microbiota Oligosaccharides Prebiotics Xylooligosaccharides

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Current therapeutic modalities and chemopreventive role of natural products in liver cancer:Progress and promise

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World Journal of Hepatology 2023年第1期15卷 1-18页

作者： amit kumar singh Shiv Vardan singh Ramesh kumar Shashank kumar Sabyasachi Senapati Abhay K Pandey Department of Botany Government Naveen Girls CollegeBalod(Hemchand Yadav University)DurgChattisgarhIndia Department of Biochemistry University of AllahabadPrayagraj 211002Uttar PradeshIndia Department of Biochemistry School of Basic and Applied SciencesCentral University of PunjabBathinda 151401PunjabIndia Department of Human Genetics and Molecular Medicine Central University of PunjabBathinda 151401PunjabIndia

Liver cancer is a severe concern for public health officials since the clinical cases are increasing each year,with an estimated 5-year survival rate of 30%–35%after *** carcinoma(HCC)constitutes a significant subtype of liver cancer(approximate75%)and is considered primary liver *** for liver cancer mainly depends on the stage of its progression,where surgery including,hepatectomy and liver transplantation,and ablation and radiotherapy are the prime *** advanced liver cancer,various drugs and immunotherapy are used as first-line treatment,whereas second-line treatment includes chemotherapeutic drugs from natural and synthetic *** and lenvatinib are first-line therapies,while regorafenib and ramucirumab are secondline *** metabolic and signaling pathways such as Notch,JAK/STAT,Hippo,TGF-β,and Wnt have played a critical role during HCC *** has also been implicated in liver ***-induced toxicity is a key obstacle in the treatment of liver cancer,necessitating the development of effective and safe medications,with natural compounds such as resveratrol,curcumin,diallyl sulfide,and others emerging as promising anticancer *** review highlights the current status of liver cancer research,signaling pathways,therapeutic targets,current treatment strategies and the chemopreventive role of various natural products in managing liver cancer.

关键词： Liver cancer Hepatocellular carcinoma Signaling pathways Therapeutic targets Natural products Chemopreventive

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Pitting corrosion resistance and bond strength of stainless steel overlay by friction surfacing on high strength low alloy steel

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Defence Technology（防务技术） 2015年第3期11卷 299-307页

作者： amit kumar singh G.Madhusudhan REDDY K.Srinivas RAO Defence Metallurgical Research Laboratory Andhra University

Surface modification is essential for improving the service properties of components. Cladding is one of the most widely employed methods of surface modification. Friction surfacing is a candidate process for depositing the corrosion resistant coatings. Being a solid state process, it offers several advantages over conventional fusion based surfacing process. The aim of this work is to identify the relationship between the input variables and the process response and develop the predictive models that can be used in the design of new friction surfacing applications. In the current work, austenitic stainless steel AISI 304 was friction surfaced on high strength low alloy steel substrate. Friction surfacing parameters,such as mechtrode rotational speed, feed rate of substrate and axial force on mechtrode, play a major role in determining the pitting corrosion resistance and bond strength of friction surfaced coatings. Friction surfaced coating and base metal were tested for pitting corrosion by potentiodynamic polarization technique. Coating microstructure was characterized using optical microscopy, scanning electron microscopy and X-ray diffraction. Coatings in the as deposited condition exhibited strain-induced martensite in austenitic matrix. Pitting resistance of surfaced coatings was found to be much lower than that of mechtrode material and superior to that of substrate. A central composite design with three factors(mechtrode rotational speed, substrate traverse speed, axial load on mechtrode) and five levels was chosen to minimize the number of experimental conditions. Response surface methodology was used to develop the model. In the present work, an attempt has been made to develop a mathematical model to predict the pitting corrosion resistance and bond strength by incorporating the friction surfacing process parameters.

关键词：奥氏体不锈钢摩擦堆焊粘结强度低合金高强度抗蚀性钢表面耐点蚀性能

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Inaudible Sound Covert Channel with Anti-Jamming Capability: Attacks vs. Countermeasure

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Journal of Electronic Science and Technology 2022年第4期20卷 396-415页

作者： Xiao-Hang Wang Shou-Bin Li Ying-Tao Jiang amit kumar singh Bi-Yun Ma Le-Tian Huang Mei Yang Fen Guo School of Cyber Science and Technology Zhejiang UniversityHangzhou 310012 ZJU-Hangzhou Global Scientific and Technological Innovation Center Hangzhou 310012 School of Software Engineering South China University of TechnologyGuangzhou 510006 Department of Electrical and Computer Engineering University of NevadaLas Vegas 89154 School of Computer Science and Electronic Engineering University of EssexColchester CO43SQ School of Electronics and Information Engineering South China University of TechnologyGuangzhou 510640 School of Electronic Science and Engineering University of Electronic Science and Technology of ChinaChengdu 610054

When an inaudible sound covert channel(ISCC)attack is launched inside a computer system,sensitive data are converted to inaudible sound waves and then *** receiver at the other end picks up the sound signal,from which the original sensitive data can be *** a forceful countermeasure against the ISCC attack,strong noise can be used to jam the channel and literally shut down any possible sound data *** this paper,enhanced ISCC is proposed,whose transmission frequency can be dynamically ***,if the transmitter detects that the covert channel is being jammed,the transmitter and receiver both will switch to another available frequency and re-establish their communications,following the proposed communications *** results show that the proposed enhanced ISCC can remain connected even in the presence of a strong jamming noise ***,a detection method based on frequency scanning is proposed to help to combat such an anti-jamming sound *** the proposed countermeasure,the bit error rate(BER)of the data communications over enhanced ISCC soars to more than 48%,essentially shutting down the data transmission,and thus neutralizing the security threat.

关键词： Countermeasure inaudible sound covert channel(ISCC)

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Effect of friction stir processing on corrosion of Al-TiB2 based composite in 3.5 wt.% sodium chloride solution

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Transactions of Nonferrous Metals Society of China 2019年第7期29卷 1383-1392页

作者： Ritukesh SHARMA amit kumar singh amit ARORA S. PATI P. S. DE School of Minerals Metallurgical and Materials Engineering Indian Institute of Technology Bhubaneswar India Materials Science and Engineering Indian Institute of Technology Gandhinagar India

The microstructure and corrosion behavior of as-cast and friction stir processed in-situ Al-TiB2 based composite in 3.5 wt.% sodium chloride solution were investigated. The microstructure was characterized using X-ray diffractometry, scanning electron microscopy and electron backscattered diffraction technique while corrosion behavior was evaluated using linear/cyclic potentiodynamic, electrochemical impedance spectroscopy and ASTM–G67 tests. The composite contains sub-micron TiB2 particles in an aluminum matrix with both blocky and fine clusters of Al3Ti agglomerated around TiB2 and displays a low uniform corrosion rate. It is also resistant to pitting as substantiated by the absence of a positive loop in cyclic potentiodynamic tests. This is due to the non-conductive nature of TiB2 particles and a controlled amount of blocky Al3Ti phase. However, both friction stir processed and as-cast composites are susceptible to inter-granular corrosion where Al3Ti and TiB2 at grain boundaries provide initiation sites for corrosion. Electrochemical impedance study attributes this to the adverse effect of Al3Ti and TiB2 on the protective oxide surface film, which increases with immersion time.

关键词： friction stir processing corrosion behavior Al composite electrochemical impedance spectroscopy (EIS)

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高速公路强制换道持续时间半参数生存分析

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浙江大学学报（工学版） 2017年第11期51卷 2215-2221页

作者：吴江玲张生瑞 amit kumar singh 秦思孙振东长安大学公路学院陕西西安710064 Atkins North America Inc.austintxusa78758

为了分析高速公路施工作业区车辆的换道行为,运用生存分析的半参数方法,建立基于风险的车辆强制换道持续时间模型,通过无人机采集包茂高速公路陕西境内某路段施工作业区的交通数据,对道路车辆强制换道持续时间进行估计.结果表明:强制换... 详细信息

为了分析高速公路施工作业区车辆的换道行为,运用生存分析的半参数方法,建立基于风险的车辆强制换道持续时间模型,通过无人机采集包茂高速公路陕西境内某路段施工作业区的交通数据,对道路车辆强制换道持续时间进行估计.结果表明:强制换道持续过程的生存时间大于5s的概率约为80%,大于10s的概率为28%,一半以上处在5～10s之间;当距离小于400m时,换道起点至合流点距离越近,车辆强制换道持续时间越短;当距离大于400m时,车辆换道持续时间开始降低.车辆换道时间持续到t时刻,在至合流点距离50～100m范围内开始换道的车辆,在下一时刻完成换道的可能性最大.

关键词：强制换道换道持续时间施工区生存分析

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高速公路施工区车辆强制换道耗时生存模型

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哈尔滨工业大学学报 2017年第9期49卷 47-50页

作者：吴江玲张生瑞秦思 amit kumar singh 孙振东长安大学公路学院西安710064 Atkins

为研究高速公路施工区车辆强制换道行为及其影响因素,采用生存分析中的半参数分析方法建立强制换道耗时的乘法风险率模型.通过无人机拍摄采集高速公路施工区的车辆换道耗时及其影响因素数据,最终确立换道耗时Cox比例风险模型,对换道耗... 详细信息

为研究高速公路施工区车辆强制换道行为及其影响因素,采用生存分析中的半参数分析方法建立强制换道耗时的乘法风险率模型.通过无人机拍摄采集高速公路施工区的车辆换道耗时及其影响因素数据,最终确立换道耗时Cox比例风险模型,对换道耗时数据进行Cox回归建模分析.结果表明:近77%的换道车辆在10 s内完成换道;小型车和中型车经养护施工区的换道耗时未发现显著性差别;对于相同的换道耗时,平峰期的累积生存率明显低于高峰期和过渡时期,而高峰期的累积生存率最高.建立的强制换道耗时生存模型可有效的定量分析车型和交通时段对高速公路施工区车辆换道行为的影响,可为高速公路施工区交通管理控制及车辆换道行为建模及仿真奠定一定的理论基础.

关键词：高速公路车辆强制换道换道耗时生存分析施工区

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