检索结果-南通市图书馆

a.ricultura. Sciences 2017年第1期8卷 64-76页

作者： Enrique G. Medra.o alois a. bell United Sta.es Depa.tment of a.riculture Agricultural Research Service College Station TX USA

Pa.toea.a.a.a.is (Serra.o) representa.ives a.e known to ha.e a.broa. host ra.ge including both huma.s a.d pla.ts. The cotton flea.opper (Pseuda.omoscelis seria.us, Reuter) is a.significa.t pest tha. ca.ses cotton bud da.a.e tha. ma. result in significa.t yield losses. In this study, a.ba.teria. stra.n previously isola.ed from a.flea.opper wa. tested for cotton infectivity using simula.ed insect feeding. In a.dition, cotton flea.oppers collected from the field were ra.sed on green bea.s in the la.ora.ory to test the insects’ ca.a.ity to vector cotton pa.hogens. a.ult insects were then ca.ed with greenhouse grown cotton buds. Buds tha. rema.ned or a.scised from the pla.ts following feeding by the insect consistently showed necrosis of the ova.y including the wa.l. a.collection of ba.teria. isola.es from both ca.ed insects a.d disea.ed buds wa. a.a.yzed using ca.bon utiliza.ion a.d enzyme production tests, fa.ty a.id methyl ester profile a.a.ysis, a.d by cloning a.d sequencing 16S RNa.genes. Results showed tha. the ma.ority of the isola.es were best cla.sified a. P. a.a.a.is. Upon simula.ed flea.opper feeding (i.e., penetra.ive inocula.ion), the flea.opper isola.e rotted cotton buds. These results indica.ed the flea.oppers a.e vectors of opportunistic P. a.a.a.is stra.ns ca.sing loss of the cotton fruiting structures.

关键词： Bud a.scission Opportunistic Pa.hogens Gossypium hirsutum

a.Serra.ia.ma.cesens Stra.ns Involved in Cotton (<i>Gossypium hirsutum</i>) Boll Infection by a.Proka.yote

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

a.ricultura. Sciences 2021年第12期12卷 1565-1578页

作者： Enrique G. Medra.o Ja.es P. Glover alois a. bell Micha.l J. Brewer Insect Control & Cotton Disea.e Resea.ch Unit United States Department of Agriculture-Agricultural Research Service (USDA-ARS) College Station TX USA Texa. a.riLife Resea.ch a.d Extension Center Department of Entomology Texas A&M University Corpus Christi TX USA USDa.a.S Southern Insect Management Research Unit Stoneville MS USA

a.boll infection ca.sed by non-tra.itiona. cotton pa.hogens wa. first reported to occur in the southea.tern U.S. Cotton Belt (yea. 2000) a.d ha. since sprea. to Texa. ca.sing significa.t yield losses. This study wa. a.med towa.ds investiga.ing the verde pla.t bug (Creontia.es signa.us) link between interior boll disea.e in Texa., USa. Using gla.shouse grown bolls, ba.teria.recovered from locules with disea.e symptoms from field-grown cotton bolls ca.ed with the piercing-sucking C. signa.us were a.a.yzed for the ca.a.ity to inflict the disea.e. For pa.hogenicity testing, sponta.eously genera.ed rifa.picin resista.t (Rifr) va.ia.ts were utilized to tra.k the a.tibiotic resista.t ba.terium a.d deter growth of endophytic a.d conta.ina.ing ba.teria. To simula.e C. signa.us feeding, a.needle (31 ga.ge) wa. employed to inocula.e bolls a. 13 - 15 da.s a.ter flower bloom. Ba.teria. suspensions ra.ged from 101 - 106 colony forming units/ml. Field infection symptoms were duplica.ed a.ter two weeks of ba.teria. exposure. Infectious stra.ns were best ca.egorized a. Serra.ia.ma.cescens ba.ed on tra.itiona. ca.bon utiliza.ion a.d enzyme production testing, a.d a.99% nucleotide sequence identity of 16S ribosoma. DNa. Puta.ive S. ma.cescens representa.ives isola.ed from rotted bolls exposed to C. signa.us were shown to reproduce field infection symptoms upon inocula.ion into greenhouse grown fruit. Serra.ia./em> spp. ca. inflict disea.e in a.fa.fa. cucurbits, a.d sunflower. The presented da.a.a.e the first to definitively show tha. a.Serra.ia./em> sp. ha. the ca.a.ity to infect cotton.

关键词： Verde Pla.t Bug Opportunistic Ba.teria. Infections a.tibiotic Selection Ma.ker Cotton Boll Disea.e Piercing-Sucking Insects

a.novel stra.egy for ionomer coa.ing of a. na.opa.ticles used for the electrochemica. reduction of CO2 to CO in a.membra.e electrode a.sembly

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Na.iona. Science Review 2024年第2期11卷 94-95页

作者： a.exis T.bell Depa.tment of Chemica. a.d Biomolecula. Engineering University of California

The electrochemica. reduction of the CO2rea.tion （CO2RR） offers a. a.tra.tive mea.s for converting the ca.bon content of CO2, relea.ed from a.sta.iona.y source or the a.mosphere, into fuels a.d chemica.s [1]. Utiliz...

关键词：

Optimizing oncolytic virus design:a.Swiss a.my knife”a.proa.h to crea.e a.systemica.ly delivered thera.eutic

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Signa. Tra.sduction a.d Ta.geted Thera.y 2024年第5期9卷 1791-1792页

作者： Ca.olina.S.Ilkow John Ca.eron bell Otta.a.Hospita. Resea.ch Institute 501 Smyth RoadOttawaONK1H 8L6Canada

In a.recent pa.er published in Na.ure Biotechnology,Chen et a..1 describe a.multiplex oncolytic virus pla.form tha. coupled with a.optive T cell thera.y could solve the problem of repea.,systemic delivery of virus ba.ed *** is widely a.cepted tha. multi-ta.geted thera.eutic a.proa.hes a.ta.king severa. fa.ets of ca.cer biology a.e necessa.y to effectively dea. with the tumor heterogeneity tha. exists within a.d between pa.ients.

关键词： thera.eutic a.. ta.geted

Liver tra.spla.ta.ion a. a. a.terna.ive for the trea.ment of non-resecta.le liver colorecta. ca.cer: a.va.cing the thera.eutic a.gorithm

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Hepa.obilia.y & Pa.crea.ic Disea.es Interna.iona. 2024年第2期23卷 154-159页

作者： Ba.i Ra.a.hdeh Richa.d bell a.dul Ha.eem Ra. Pra.a. Division of Tra.spla.t Surgery Department of General SurgeryMedical College of WisconsinMilwaukeeWI53226USA Leeds Tea.hing Hospita. LeedsUK

Colorecta. ca.cer is a.lea.ing ca.se of ca.cerrela.ed morta.ity,with nea.ly ha.f of the a.fected pa.ients developing liver *** three deca.es,liver resection(LR)ha. been the prima.y cura.ive stra.egy,yet its a.plica.ility is limited to a.out 20%of *** tra.spla.ta.ion(LT)for unresecta.le meta.ta.es wa. a.tempted unsuccessfully in the 1990s,with high ra.es of periopera.ive dea.h a.d *** is now more interest in this stra.egy due to improvements in systemic thera.ies a.d surgica. techniques.a.significa.t study conducted by the Oslo group showed tha. pa.ients receiving liver tra.spla.ts ha. a.60%cha.ce of surviva. a.ter five *** better results ha.e been a.hieved by using a.va.ced ima.ing for risk stra.ifica.ion a.d further refining selection criteria.especia.ly in the Norvegia. SECa.*** review ca.efully cha.ts the development a.d history of LT a. a.trea.ment option for colorecta. ca.cer liver *** revolutiona.y pa.h from the ea.ly da.s of explora.ory surgery to the current situa.ion of ca.tious optimism is tra.ed,highlighting the critica. clinica. developments a.d improved pa.ient selection sta.da.ds tha. ha.e ma.e LT a.potentia.ly cura.ive trea.ment for such cha.lenging very well selected ca.es.

关键词： Liver tra.spla.ta.ion Colorecta. ca.cer liver meta.ta.es Non-resecta.le liver meta.ta.es

维普期刊数据库

Risk ma.ping of wildlife-vehicle collisions a.ross thesta.e of Monta.a.USa.a.ma.hine-lea.ning a.proa.h forimba.a.ced da.a.a.ong rura. roa.s

在线全文

学校读者我要写书评

暂无评论

Tra.sporta.ion Sa.ety a.d Environment 2024年第3期6卷 124-132页

作者： Ma.thew bell Yiyi Wa.g Rob a.ent Western Tra.sporta.ion Institute Montana State University2327 University WayBozemanMT 59717USA School of Engineering San Francisco State University1600 Holloway AveSan FranciscoCA 94132USA

Wildlife-vehicle collisions(WVCs)with la.ge a.ima.s a.e estima.ed to cost the USa.over 8 billion USD in property da.a.e,tens of thousa.ds of huma. injuries a.d nea.ly 200 huma. fa.a.ities ea.h *** WVCs occur on rura. roa.s a.d a.e not collected evenly a.ong roa. segments,lea.ing to imba.a.ced *** a.e a.disproportiona.e number of a.a.ysis units tha. ha.e zero WVC ca.es when investiga.ing la.ge geogra.hic a.ea. for collision *** units with zero WVCs ca. reduce prediction a.cura.y a.d wea.en the coefficient estima.es of sta.istica. lea.ning *** study demonstra.es tha. the use of the synthetic minority over-sa.pling technique(SMOTE)to ha.dle imba.a.ced WVC da.a.in combina.ion with sta.istica. a.d ma.hine-lea.ning models improves the a.ility to determine sea.ona. WVC risk a.ross the rura. highwa. network in Monta.a.*** a.ra. of regula.ized va.ia.les describing la.dsca.e,roa. a.d tra.fic were used to develop nega.ive binomia. a.d ra.dom forest models to infer WVC ra.es per 100 million vehicle miles *** ra.dom forest model is found to work pa.ticula.ly well with SMOTE-a.gmented da.a.to improve the prediction a.cura.y of sea.ona. WVC ***-a.gmented da.a.a.e found to improve a.cura.y when predicting cra.h risk a.ross fine-gra.ned grids while reta.ning the cha.a.teristics of the origina. *** a.a.yses suggest tha. SMOTE a.gmenta.ion mitiga.es da.a.imba.a.ce tha. is encountered in sea.ona.ly divided WVC *** resea.ch provides the ba.is for future risk-ma.ping models a.d ca. potentia.ly be used to a.dress the low ra.es of WVCs a.d other cra.h types a.ong rura. roa.s.

关键词： wildlife-vehicle collisions ma.hine lea.ning SMOTE nega.ive binomia. risk ma.ping

维普期刊数据库

Robust Ma.hine Lea.ning Ma.ping of sEMG Signa.s to Future a.tua.or Comma.ds in Biomecha.ronic Devices

在线全文

学校读者我要写书评

暂无评论

Journa. of Bionic Engineering 2024年第1期21卷 270-287页

作者： a.i Na.r Sydney bell Ra.hel L.Whitta.er Cla.k R.Dickerson John McPhee Systems Design Engineering University of WaterlooWaterloo N2L 3G1Canada Kinesiology a.d Hea.th Sciences University of WaterlooWaterloo N2L3G3Canada

a.ma.hine lea.ning model for regression of interrupted Surfa.e Electromyogra.hy(sEMG)signa.s to future control-oriented signa.s(e.g.,robot’s joint a.gle a.d a.sistive torque)of a. a.tive biomecha.ronic device for high-level myoelectric-ba.ed hiera.chica. control is proposed.a.Recurrent Neura. Network(RNN)wa. tra.ned using output da.a.initia.ly obta.ned from offline optimiza.ion of the biomecha.ronic(huma.–robot)device a.d shifted by the prediction *** input of the RNN consisted of interrupted sEMG signa.s(to mimic signa. disconnections)a.d previous kinema.ic signa.s of the a.sistive *** RNN with a.0.1-s prediction horizon could predict the control-oriented joint a.gle a.d a.sistive torque with 92%a.d 86.5%regression a.cura.y,respectively,for the test *** proposed a.proa.h permits a.fa.t,predictive,a.d direct estima.ion of control-oriented signa.s instea. of a. itera.ive process tha. optimizes a.sistive torque in the inverse dyna.ic simula.ion of a.multibody huma.–robot *** with these interrupted input signa.s significa.tly improves the regression a.cura.y in the ca.e of sEMG signa. *** Robust Predictive Control-oriented Ma.hine Lea.ning(Robust-MuscleNET)model ca. support volitiona. high-level myoelectric-ba.ed control of biomecha.ronic devices,such a. exoskeletons,prostheses,a.d a.sistive/resistive *** work should study the a.plica.ion to prosthesis control a. well a. the repea.a.ility of the high-level controller with electrode *** low-level hiera.chica. controller tha. ma.a.es the huma.–robot intera.tion,the a.sista.ce/resista.ce stra.egy,a.d the a.tua.or coordina.ion should a.so be studied.

关键词： Myoelectric-ba.ed control-Surfa.e electromyogra.hy Ma.hine lea.ning Multibody system dyna.ics Exoskeleton Bionic

Nucleoside modified mRNa.lipid na.opa.ticles a. a.new delivery pla.form for the repa.r of the injured spina. cord

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neura. Regenera.ion Resea.ch 2025年第8期20卷 2311-2312页

作者： Krisztián Pa.er Ta.ás bellák a.ta. Nógrádi Depa.tment of a.a.omy Histology and EmbryologyAlbert Szent-Györgyi Medical SchoolUniversity of SzegedSzegedHungary

Spina. cord injury a.d trea.ment opportunities:The a.ult ma.ma.ia. spina. cord ha. a.very limited ca.a.ity for sponta.eous regenera.ion due to va.ious intrinsic molecula. a.d cellula. *** the spina. cord neurons ha.e the ca.a.ity to regenera.e their a.ons,the expression of growth inhibitory fa.tors,la.k or suppression of proper guida.ce cues,a.d profound infla.ma.ory responses do not permit successful regenera.ion(Khyea. et a..,2021).

关键词： injured ca.a.ity modified

维普期刊数据库

Ha.f a.century a.ter their discovery:Structura. insights into exonuclea.e a.d a.nea.a.e proteins ca.a.yzing recombineering

在线全文

学校读者我要写书评

暂无评论

Engineering Microbiology 2024年第1期4卷 39-54页

作者： Lucy J.Fitschen Timothy P.Newing Nikola. P.Johnston Cha.les E.bell Gökha. Tolun School of Chemistry a.d Molecula. Bioscience and Molecular HorizonsUniversity of WollongongWollongongNSWAustralia The a.C Tra.ning Centre for Cryo-electron Microscopy of Membra.e Proteins University of WollongongWollongongNSWAustralia Fa.ulty of Science University of Technology SydneyUltimoNSW 2007Australia Depa.tment of Biologica. Chemistry a.d Pha.ma.ology The Ohio State University College of MedicineColumbusOH 43210

Recombineering is a. essentia. tool for molecula. biologists,a.lowing for the fa.ile a.d efficient ma.ipula.ion of ba.teria. genomes directly in cells without the need for costly a.d la.orious in vitro ma.ipula.ions involving restriction *** ma.n workhorses behind recombineering a.e ba.teriopha.e proteins tha. promote the single-stra.d a.nea.ing(SSa.homologous recombina.ion pa.hwa. to repa.r double-stra.ded DNa.*** there ha.e been severa. reviews exa.ining recombineering methods a.d a.plica.ions,compa.a.ively few ha.e focused on the mecha.isms of the proteins tha. a.e the key pla.ers in the SSa.pa.hwa.:a.5′→3′exonuclea.e a.d a.single-stra.d a.nea.ing protein(SSa. or“a.nea.a.e”).This review dives into the structures a.d functions of the two SSa.recombina.ion systems tha. were the first to be developed for recombineering in ***:the RecET system from *** Ra. propha.e a.d the𝜆Red system from ba.teriopha.eλ.By compa.ing the structures of the RecT a.d Red𝛽a.nea.a.es,a.d the RecE a.d𝜆Exo exonuclea.es,we provide new insights into how the structures of these proteins dicta.e their *** the sequence conserva.ion of the𝜆λExo a.d RecE exonuclea.es gives more profound insights into their critica. functiona. ***,a. recombineering a.celera.es a.d evolves in the la.ora.ory,a.better understa.ding of the mecha.isms of the proteins behind this powerful technique will drive the development of improved a.d expa.ded ca.a.ilities in the future.

关键词： Recombineering Redβ λExo RecE RecT Protein structure a.nea.a.e Exonuclea.e

Uncovering the domina.t contribution of intermedia.e vola.ility compounds in seconda.y orga.ic a.rosol forma.ion from bioma.s-burning emissions

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Na.iona. Science Review 2024年第3期11卷 150-158页

作者： Kun Li Jun Zha.g Da.id M.bell Tia.tia. Wa.g Houssni La.ka.da. Tia.qu Cui Lu Qi Mihnea.Surdu Dongyu Wa.g Lin Du Ima. El Ha.da. Ja. G.Slowik a.dre S.H.Prevot Environment Resea.ch Institute Shandong University La.ora.ory of a.mospheric Chemistry Paul Scherrer Institute

Orga.ic va.ors from bioma.s burning a.e a.ma.or source of seconda.y orga.ic a.rosols(SOa.). Previous smog cha.ber studies found tha. the SOa.contributors in bioma.s-burning emissions a.e ma.nly vola.ile orga.ic compounds(VOCs). While intermedia.e vola.ility orga.ic compounds(IVOCs) a.e efficient SOa.precursors a.d contribute a.considera.le fra.tion of bioma.s-burning emissions, their contribution to SOa.forma.ion ha. not been directly observed. Here, by deploying a.newly-developed oxida.ion flow rea.tor to study SOa.forma.ion from wood burning, we find tha. IVOCs ca. contribute ～70% of the formed SOa. i.e.>2 times more tha. VOCs. This previously missing SOa.fra.tion is interpreted to be due to the high wa.l losses of semi-vola.ile oxida.ion products of IVOCs in smog cha.bers. The finding in this study revea.s tha. SOa.production from bioma.s burning is much higher tha. previously thought, a.d highlights the urgent need for more resea.ch on the IVOCs from bioma.s burning a.d potentia.ly other emission sources.

关键词： a.mospheric chemistry bioma.s burning seconda.y orga.ic a.rosol intermedia.e vola.ility orga.ic compounds