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Food Science and Human Wellness 2019年第2期8卷 142-148页

作者： Emmanuel Anyachukwu Irondi Bosede Mofoluwake Adegoke Emy Sofie Effion Selimat Oluwakemi Oyewo Emmanuel Oladeji Alamu aline augusti boligon Department of Biochemistry Kwara State UniversityMaleteP.M.B.1530IlorinNigeria Department of Applied Sciences Osun State PolytechnicIreeOsun StateNigeria International Institute of Tropical Agriculture(IITA) Grosvenor HouseCroydonCR09 XPUnited Kingdom Phytochemical Research Laboratory Department of Industrial PharmacyFederal University of Santa MariaSanta MariaCEP 97105-900Brazil

Whole grain cereals are important dietary sources for management of metabolic diseases due to the bioactive components they ***,this study investigated enzymes(pancreatic lipase,-amylase,-glucosidase,xanthine oxidase and angiotensin 1-converting enzyme)inhibitory property,antioxidant activity and phenolics profile of raw and roasted red sorghum(Sorghum bicolor)grains in *** of flours of raw and roasted(150◦C and 180◦C,for 20 min)grains were assayed for enzymes inhibitory and antioxidant activities using spectrophotometric methods;while their phenolic constituents were characterized using *** raw grains exhibited strong enzymes inhibitory and antioxidant activities,and contained phenolic acids(gallic,chlorogenic,caffeic,ellagic and p-coumaric acids)and flavonoids(quercetin,luteolin and apigenin).However,whereas the enzymes inhibitory activity and levels of the phenolic compounds in the grains decreased significantly(p<0.05)with increasing roasting temperature,the antioxidant activity ***,roasting at high temperature may not be recommended for the optimum retention of the enzymes inhibitory property and phenolic compounds of red sorghum grains.

关键词： Antioxidant activity Enzymes inhibition Metabolic diseases Phenolic compounds Sorghum grains

Phenolic composition and inhibitory activity of Mangifera indica and Mucuna urens seeds extracts against key enzymes linked to the pathology and complications of type 2 diabetes

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Asian Pacific Journal of Tropical Biomedicine 2014年第11期4卷 903-910页

作者： Emmanuel Anyachukwu Irondi Ganiyu Oboh Afolabi Akintunde Akindahunsi aline augusti boligon Margareth Linde Athayde Department of Biochemistry Federal University of Technology.P.M.B.704Akure 340001Nigeria Biochemistry Unit Department of Biosciences and BiotechnologyKwara State UniversityMaleteP.M.B.1530IlorinNigeria Phytochemical Research Laboratory Department of Industrial PharmacyFederal University of Santa MariaBuilding 26.Room 1115.Santa Maria.CEP 97105-900.Brazil

Objective:To investigate the phenolic compounds composition and the inhibitory activity of Mangifera indica(***) and Mucuna urens(***) seeds extracts against some key enzymes(-amylase,-glucosidasc and aldose reductase) implicated in the pathology and complications of type 2 diabetes in ***:Reverse phase chromatographic quantification of the major flavonoids and phenolic acids in the seeds extracts was carried out using high performance liquid chromatography coupled with diode array *** inhibitory activities of the seeds extracts against-amylase and-glucosidase were estimated using soluble starch and p-nitrophenylglucopyranosidc as their respective *** of aldose reductase activity by the extracts was assayed using partially purified lens homogenate of normal male rat as source of enzyme;inhibition of Fe^(2+)-induced lipid peroxidation by extracts was tested in rat pancreas ***:The chromatography result revealed that extracts of both seeds had appreciable levels of some major flavonoids and phenolic acids of pharmacological importance,including gallic acid,chlorogenic acid,caffeic acid,ellagic acid,catechin,rutin,*** and *** of both seeds effectively inhibited-amylase,-glucosidase and aldose reductase activities in a dose-dependent manner,having inhibitory preference for these enzymes in the order of aldose reductase>-glucosidase>-*** lower half-maximal inhibitory concentrations(IC50)against-amylase,-glucosidase,and aldose reductase,*** had stronger inhibitory potency against these enzymes than *** of both seeds also inhibited Fe2+-induced lipid peroxidation in a dose-dependent pattern,with *** being more potent than ***:The results obtained provide support for a possible use of *** and *** seeds in managing hyperglycemia and preventing the complications associated with it in type 2diabetes.

关键词： Hyperglycemia Diabetic complications Enzyme inhibition Lipid peroxidation Mangifera indica Mucuna urens

Phenolics extract of Tetrapleura tetraptera fruit inhibits xanthine oxidase and Fe^2+-induced lipid peroxidation in the kidney,liver,and lungs tissues of rats in vitro

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Food Science and Human Wellness 2016年第1期5卷 17-23页

作者： Emmanuel Anyachukwu Irondi Ganiyu Oboh Samson Olalekan Agboola aline augusti boligon Margareth Linde Athayde Biochemistry Unit Department of Biosciences and BiotechnologyKwara State UniversityMaleteP.M.B.1530IlorinNigeria Functional Foods and Nutraceuticals Unit Department of BiochemistryFederal University of TechnologyAkureNigeriaP.M.B.704Akure 340001Nigeria Department of Veterinary Physiology Biochemistry and PharmacologyUniversity of IbadanNigeria Phytochemical Research Laboratory Department of Industrial PharmacyFederal University of Santa MariaBuilding 26Room 1115Santa Maria CEP 97105-900Brazil

The phenolics composition and inhibitory activity of Tetrapleura tetraptera fruit extract on xanthine oxidase(XO)and Fe2+-induced lipid peroxidation in the kidney,liver and lungs tissues of rats were evaluated in ***(flavonoids and phenolic acids)were quantified using reverse-phase high performance liquid chromatrography coupled with diode array detection(HPLC-DAD).The XO and Fe2+-induced lipid peroxidation inhibitory abilities of the extract were evaluated using spectrophotometric *** extract contained some flavonoids and phenolic acids,including catechin,epicatechin,rutin,quercetin,luteolin,apigenin;gallic,chlorogenic,caffeic and ellagic acids that are beneficial to *** extract inhibited XO in the kidney,liver and lungs tissues in a dose-dependent *** half-maximal inhibitory concentrations(IC50)of the extract on XO from the tissues varied significantly(Pkidney>*** extract also inhibited Fe2+-induced lipid peroxidation in a dose-dependent pattern,having IC50 in the order of liver>lungs>*** fruit extract could be a promising nutraceutical for preventing and managing hyperuricaemia and the associated disease conditions,due to its ability to inhibit XO;this bioactivity is attributable to combined effect of its flavonoids and phenolic acids.

关键词： peroxidation kidney inhibited

Chemical profile and antimicrobial activity of Secondatia floribunda A. DC(Apocynaceae)

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Asian Pacific Journal of Tropical Biomedicine 2017年第8期7卷 739-749页

作者： Daiany Alves Ribeiro Sarah Soares Damasceno aline augusti boligon Irwin Rose Alencar de Menezes Marta Maria de Almeida Souza JoséGalberto Martins da Costa Programa de Pós-Graduacao em Etnobiologia e Conservacao da Natureza Universidade Regional do Cariri Departamento de Química Biológica Laboratório de Pesquisas de Produtos NaturaisUniversidade Regional do Cariri Laboratório de Fitoquímica Universidade Federal de Santa Maria Departamento de Química Biológica Laboratório de Farmacologia e Química MolecularUniversidade Regional do Cariri Departamento de Ciências Biológicas Laboratório de Ecologia VegetalUniversidade Regional do Cariri

Objective: To establish the chemical profile, and to evaluate the antibacterial and modulatory activity of the ethanolic extracts of the stalk's inner bark and heartwood of Secondatia ***: Quantification of total plienols and flavonoids was determined by the FolinCiocalteu method and aluminum chloride, respectively. Phenolic compounds were identified and quantified by HPLC-DAD(High Performance Liquid ChromatographyDiodearray Detector) and the Infrared Spectroscopy was performed using the measure by Attenuated Total Reflectance with Fourier Transform(ATR-FTIR). Antibacterial assays for determination of the Minimum Inhibitory Concentration(MIC) and modification of aminoglycosides were performed by ***: Infrared spectra showed similar characteristics, having among its main absorption bands hydroxyl group(OH). The antibacterial activity showed clinically significant results for the strains of Staphylococcus aureus and Escherichia coli. In modulation assay, synergic and antagonistic effect for both extracts was *** extract in combination with antibiotics showed a significant MIC reduction at 19.8%(P<0.0001)-79.3%(P<0.01).Conclusions: This study is the first report of chemical and biological information of Secondatia floribunda suggesting that it is clinically relevant source of a new antibacterial therapy, especially due to the presence of significant levels of phenolic compounds.

关键词：化学作文 HPLC Cinchonain 自然产品修正抵抗

Phenolics from grapefruit peels inhibit HMG-CoA reductase and angiotensin-I converting enzyme and show antioxidative properties in endothelial *** 926 cells

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Food Science and Human Wellness 2015年第2期4卷 80-85页

作者： Ayokunle O.Ademosun Ganiyu Oboh Sabina Passamonti Federica Tramer Lovro Ziberna aline augusti boligon Margareth Linde Athayde Functional Foods and Nutraceuticals Unit Department of BiochemistryFederal University of TechnologyAkureNigeria Department of Life Sciences University of Triestevia L.Giorgieri 134127 TriesteItaly Program of Post-Graduation in Pharmaceutical Sciences Federal University of Santa MariaCampus CamobiSanta MariaRS 97105-900Brazil

This study sought to investigate the possible mechanisms for the use of phenolic extracts from grapefruit peels in the management/prevention of cardiovascular *** effects of the phenolic extracts on key enzymes relevant to cardiovascular diseases[3-hydroxy-methyl-3-glutaryl coenzyme A reductase(HMG-CoA reductase)and angiotensin-I converting enzyme(ACE)],cellular antioxidant activity in human endothelial cells(*** 926)and radicals[1,1-diphenyl-2 picrylhydrazyl(DPPH)and 2,2-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid)(ABTS)]scavenging abilities were *** phenolic contents of the extracts were investigated using HPLC–*** was no significant(P>0.05)difference in the HMG-CoA reductase inhibitory ability of the two extracts,while the bound phenolic extracts had a stronger ACE inhibitory ability than the soluble free *** extracts also showed intracellular antioxidant activity in human endothelial(*** 926)***,the bound phenolics had significantly higher radicals(DPPH*and ABTS*)scavenging abilities than the free *** HPLC analysis revealed the presence of flavonoids(quercetin and kaempferol),phenolics acids(resveratrol,gallic acid,ellagic acid and caffeic acid)and tannin(catechin).The cellular antioxidative properties and inhibition of enzymes relevant to the management of cardiovascular complications showed that grapefruit peels could be used as nutraceuticals for the management of such conditions.

关键词： prevention cardiovascular phenolic

Ethylacetate extract of red onion(Allium cepa L.)tunic affects hemodynamic parameters in rats

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Food Science and Human Wellness 2015年第3期4卷 115-122页

作者： Olanrewaju Sam Olayeriju Mary Tolulope Olaleye Olamide Olajusi Crown Kayode Komolafe aline augusti boligon Margareth Linde Athayde Akintunde Afolabi Akindahunsi Department of Biochemistry School of SciencesThe Federal University of TechnologyPMB 704AkureNigeria Department of Biochemistry Faculty of ScienceFederal University Oye-EkitiPMB 373Oye-EkitiNigeria Department of Industrial Pharmacy Federal University of Santa Maria97105-900 Santa MariaRSBrazil

The effects of ethylacetate extract of red onion(Allium cepa)tunic(ACTE)on some hemodynamic and biochemical parameters were evaluated in normotensive albino *** pressure parameters were determined in anaesthetized rats orally administered ACTE(10-,20-,or 40 mg/kg)or ramipril(1 mg/kg)once daily for 14 ***,10-,20-,or 40 mg/kg ACTE produced significant(P<0.01),dose-dependent fall in systolic blood pressure,SBP(21%,27%,33%),diastolic blood pressure,DBP(6%,10%,16%),pulse pressure,PP(42%,49%,56%),mean arterial blood pressure,MAPB(13%,18%,23%)and heart rate,HR(4%,5%,7%).The highest effective dose(40 mg/kg)compared well with ramipril(1 mg/kg)with regards to SBP(41%),DBP(19%),PP(70%),MABP(29%)and HR(10%).Similar trends(decreases)were recorded for 40 mg/kg ACTE and ramipril,respectively,as regards the activities of serum enzymes:creatine kinase(60%and 65%),ALT(18%and 14%)and ALP(28%and 16%).HPLC fingerprints of the flavonoid-rich ACTE revealed that flavonols:quercetin,quercitrin,isoquercitrin,rutin and kaempferol are the active *** results demonstrate the hypotensive effect of *** tunic flavonoids initiating further investigation of their individual or synergistic contribution(s)to the observed effects.

关键词： acetate Blood rats

Antiulcerogenic activity of Scutia buxifolia on gastric ulcers induced by ethanol in rats

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Acta Pharmaceutica Sinica B 2014年第5期4卷 358-367页

作者： aline augusti boligon Robson Borba de Freitas Thiele Faccim de Brum Emily Pansera Waczuk Cláudia Vargas Klimaczewski Daiana Silva deÁvila Margareth Linde Athayde Liliane de Freitas Bauermann Department of Industrial Pharmacy Center of Health SciencesFederal University of Santa MariaSanta Maria 97105-900RSBrazil Department of Physiology and Pharmacology Center of Health SciencesFederal University of Santa MariaSanta Maria 97105-900RSBrazil Department of Biochemistry Federal University of PampaUruguaiana 97500-970RSBrazil

Gastric ulcers affect many people around the world and their development is a result of the imbalance between aggressive and protective factors in the gastric *** buxifolia,commonly known as coronilha,has attracted the interest of the scientific community due to its pharmacological properties and its potential therapeutic *** this study,the preventive effects of the crude extract of Scutia buxifolia(ceSb)against gastric ulcer induced by 70%ethanol were evaluated in male Wistar *** addition,the composition of ceSb was clarified by high-performance liquid chromato-graphy(HPLC).*** extract(100,200 and 400 mg/kg body weight)attenuated oxidative and histopathological features induced by ***,all evaluated doses of ceSb caused significant(P<0.001 and P<0.0001)and dose-dependent increase in sulfhydryl groups(NPSH)levels,catalase(CAT)and superoxide dismutase(SOD)***,the administration of ceSb reversed the increase in lipid peroxidation produced by *** protective effect of the extract could be attributed to antioxidant compounds present in the ceSb,such as flavonoids and phenolic acids,which were quantified by ***,an antioxidant effect of the extract leads to a protection on gastric *** results indicate that *** could have a beneficial role against ethanol toxicity by preventing oxidative stress and gastric tissue injury.

关键词： Scutia buxifolia Antioxidant Gastric ulcer HPLC

Polyphenolic composition, antibacterial, modulator and neuroprotective activity of Tarenaya spinosa(Jacq.) Raf.(Cleomaceae)

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Asian Pacific Journal of Tropical Biomedicine 2019年第1期9卷 12-17页

作者： Francisca Samara Muniz dos Santos Jose Weverton Almeida Bezerra Jean Paul Kamdem aline augusti boligon Marli Matiko Anraku Ana Raquel Pereira da Silva Kleber Ribeiro Fidelis Nadghia Figueiredo Leite Antonio Ivanildo Pinho Henrique Douglas Melo Coutinho Jose Edilson Goncalves dos Santos Microscopy Laboratory Regional University of Cariri (URCA) Program Post graduate in Plant Biology (UFPE) Phytochemical Laboratory Department of Industrial Pharmacy Federal University of Santa Maria (UFSM) Microbiology and Molecular Biology Laboratory Regional University of Cariri (URCA)

Objective: To evaluate the antibacterial activity and neuroprotective capacity of the ethanolic and aqueous extracts of Tarenaya spinosa(T. spinosa) as well as to determine and quantify some of its polyphenols by high performance liquid chromatography with diode-array detection(HPLC-DAD). Methods: The bacterial Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa strains, grown in Heart Agar Infusion, were tested. The drugs gentamicin, norfloxacin and imipenem were used to evaluate the modulating or antagonistic capacity of the T. spinosa extracts. The extract was analysed by HPLC-DAD to determine the main phenolic compounds. For the cell viability tests, individual heads of the Nauphoeta cinerea arthropod model were removed, homogenized in Trifluoromethyl ketone and centrifuged afterwards. Subsequently, 20 μL of NaN O2 were added to the biological material, except in the control group, to evaluate the protection capacity of the extracts. The homogenate of the insect heads was incubated for 2 h in tubes containing tetrazolium bromide. Results: HPLC-DAD demonstrated that the ethanolic extract of T. spinosa presented caffeic acid as the major compound. The ethanolic extract also showed neuroprotective effects at concentrations ≥ 10 μg/mL, while aqueous extract was shown to have a protective effect only at the concentration of 100 μg/mL. The aqueous extract demonstrated a clinically relevant antibacterial activity against the Staphylococcus aureus multidrug resistant strain-MDR, with MIC 512 μg/m L. However, when the extracts were associated with gentamicin and imipenem, a synergism was detected against Staphylococcus aureus and Escherichia coli MDR strains. Conclusions: Although it does not present an antibacterial action, the extracts of T. spinosa can be used in the pharmaceutical industries since its extracts show modulating action of drugs. Besides, these natural products have neuroprotective capacity.

关键词： Tarenaya spinosa Cleome spinose Nauphoeta cinerea Synergism Antibiotic resistance

Protective Role of Golden Flaxseed (Linum usitatissimum L.) Against Oxidative Damage in Lipids and Proteins of Healthy Volunteers

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Journal of Biosciences and Medicines 2015年第10期3卷 45-53页

作者： Luisa Zuravski Ritiele Pinto Coelho Jonathaline Apollo Duarte Manoelly Oliveira Rocha Juliana Mezzomo Bruna Cocco Pilar Leandro Leal Galarca Margareth Linde Athayde aline augusti boligon Michel Mansur Machado Vanusa Manfredini Postgraduate Program in Biochemistry Federal University of PampaUruguaianaBrazil Course of Pharmacy Federal University of PampaUruguaianaBrazil Postgraduate Program in Pharmaceutical Sciences Federal University of PampaUruguaianaBrazil Postgraduate Program in Pharmaceutical Sciences Federal University of Santa MariaSanta MariaBrazil

Flaxseed (Linum usitatissimum L.) is composed mainly of bioactive components such as polyphenols, polyunsaturated fatty acids, fiber and lignans. Flaxseed can be found in different presentation forms (grain or flour) and varieties (brown or golden);however, questions have arisen as to whether the presentation form and/or variety may influence the health effects. The objective of this study was to evaluate the effects on blood pressure, anthropometric and oxidative parameters in healthy human volunteers. All subjects received 40 gram aliquots of flaxseed and were instructed to consume them in their entirety mixed with water in the morning for a period of 14 days. Oxidative parameters showed significant reductions (p < 0.05) in oxidative damage to lipids and proteins via dietary intervention with golden flaxseed grains. There were no significant differences in anthropometric parameters, blood pressure, DNA damage and micronuclei frequency after 14-day supplementation. This research indicates that golden flaxseed grains can be a valuable adjunct for disease prevention and protecting the organism against oxidative damage.

关键词： Golden Flaxseed Grains Antioxidant Oxidative Damage

In vitro neuroprotective potentials of aqueous and methanol extracts from Heinsia crinita leaves

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Food Science and Human Wellness 2016年第2期5卷 95-102页

作者： Ganiyu Oboh Esther E.Nwanna Sunday I.Oyeleye Tosin A.Olasehinde Opeyemi B.Ogunsuyi aline A.boligon Functional Foods and Nutraceuticals Unit Department of BiochemistryFederal University of TechnologyAkureNigeria Nutrition and Toxicology Division Food Technology DepartmentFederal Institute of Industrial Research OshodiP.M.B.21023 LagosNigeria Phytochemical Research Laboratory Department of Industrial PharmacyFederal University of Santa MariaBuild 26Room 1115Santa Maria CEP 97105-900Brazil

This study was designed to determine the neuroprotective potentials of aqueous and methanol extracts from Heinsia crinita leaves in *** total phenol and flavonoid contents of the extracts were determined using colorimetric method while phenolic characterization of the leaf was analyzed via high performance liquid chromatography-diode array detector(HPLC-DAD).The effects of the extracts on Fe^2+-induced lipid peroxidation in rats’brain homogenate,monoamine oxidase(MAO),Na^+/K^+-ATPase,acetylcholinesterase(AChE)and butyrylcholinesterase(BChE)activities were also *** aqueous extract had higher total phenol and flavonoid contents than the methanol ***-DAD revealed that quercetin ellagic,chlorogenic and caffeic acids were the most abundant phenolic compounds in the *** aqueous extract had higher inhibitory effects on MAO,AChE and BChE activities while there was no significant difference between their Fe^2+-induced lipid peroxidation inhibitory ***,both extracts stimulated Na^+/K^+-ATPase activity;however,methanol extract had higher stimulatory *** neuroprotective properties of *** leaves could be associated with its inhibitory effects on Fe2+-induced lipid peroxidation and modulation of MAO,Na^+/K^+-ATPase,AChE,and BChE ***,*** leaves could be used as a functional food and dietary intervention for the management of some neurodegenerative ***,the aqueous extracts exhibited better neuroprotective properties.

关键词： Heinsia crinita Neurodegeneration Oxidative stress Malondialdehyde Polyphenols