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Can microcalcifications' characteristics predict the risk of breast cancer metastasis to bone?

Journal of Cancer Metastasis and Treatment 2019年第4期4卷 31-37页

作者： Rita Bonfiglio Manuel Scimeca alessandro Polidori Clara Nazzaro Giselda De silva ElenaBonanno Department of Experimental Medicine University of Rome“Tor Vergata”Via Montpellier 1Rome 00133Italy Department of Biomedicine and Prevention University of Rome“Tor Vergata”Via Montpellier 1Rome 00133Italy San Raffaele University Via di Val Cannuta 247Rome 00166Italy Assing S.p.a Via Edoardo Amaldi 14Monterotondo 00015Italy Diagnostica Medica’&“Villa dei Platani” Neuromed GroupAvellino 83100Italy

Aim: To correlate the microcalcifications' characteristics, such as morphology and elemental compositions, with the occurrence of bone metastatic lesions at 5 years from diagnosis. Methods: In this retrospective study, we enrolled 70 patients from which we collected one breast biopsy each. From each biopsy, paraffin serial sections were obtained to perform histological classifications, immunohistochemical analyses and Energy Dispersive X-ray evaluation. Results: Microcalcifications analysis showed a significant association between the presence of calcium crystals made of magnesium substituted hydroxyapatite and the development of bone metastasis from 5 years from diagnosis. No significant association was observed by evaluation the morphological appearance of microcalcifications. Immunohistochemical analysis displayed a significant association between the expression of bone morphogenetic proteins 2 and pentraxin-3, two osteoblast induction factors, and the formation of bone metastatic lesions. Conclusion: Results here reported highlighted the possible use of breast microcalcifications as a negative prognostic marker of bone metastatic diseases. In particular, the association between elemental composition of breast microcalcifications and the formation of bone lesions can lay the foundation for the development of new in vivo diagnostic tools based on the analysis of microcalcifications and capable to predict the formation of bone metastasis.

关键词： Microcalcifications breast cancer bone metastasis breast osteoblast-like cells bone morphogenetic proteins 2 pentraxin-3

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Hydrodynamics and suspended particulate matter retention in macrotidal estuaries located in Amazonia-semiarid interface(Northeastern-Brazil)

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International Journal of Sediment Research 2020年第4期35卷 417-429页

作者： Vinicius Henrique Maciel dos Santos Francisco Jose da silva Dias Audalio Rebelo Torres Romulo Araujo Soares Lais costa Terto Antonio Carlos Leal de Castro Ricardo Luvizotto Santos Marco Valerio Jansen Cutrim Laboratorio de Hidrodinamica Costeira Estuarina e de Aguas Interiores (LHiCEAI) Do Programa de Pos-Graduacao Em Oceanografia (PPGOceano) da Universidade Federal Do Maranhao (UFMA)Cidade Universitaria Dom Delgado Laboratorio de Ictiologia (LabIctio) da Universidade Federal Do Maranhao (UFMA) Cidade Universitaria Dom Delgado Laboratorio de Ecotoxicologia (LabEcotox) Do Programa de Pos-Graduacao Em Oceanografia (PPGOceano) da Universidade Federal Do Maranhao (UFMA) Cidade Universitaria Dom Delgado Laboratorio de Ficologia (LabFic) Do Programa de Pos-Graduacao Em Oceanografia (PPGOceano) da Universidade Federal Do Maranhao (UFMA) Cidade Universitaria Dom Delgado

The aim of the current study was to determine the nature of the seasonal variability of the Suspended Particulate Matter(SPM) fluxes from the drainage basin to the estuary in a macrotidal region(Northeastern Brazil),and the estuarine response to a seawater intrusion regarding sediment deposition,which will support the understanding of the global transport of materials at the continent-ocean *** structure data was acquired using a Conductivity,Temperature,and Depth(CTD) probe with a sampling frequency of 4 *** particulate material was measured by gravimetric measurements applied to exact filtered volume *** outflows were measured through the use of an Acoustic Doppler Current Profiler(ADCP) with frequency of 1.5 *** horizontal thermal and saline gradients varied from warmer and less saline waters(2014) to cooler and saline waters(2015).The gradient behavior when linked to volume transport and SPM flows,suggests a minimization of the fluvial flows in 2015,easing the advance of coastal water(CW) towards the inner estuary,leading to an inversion of the baroclinic pressure *** bottom saline front,generated by the entrance of coastal water masses,caused an increase in SPM concentrations due to increased fluid density,resuspension of previously deposited sediment,and erosion of *** concentrations of SPM indicate higher volume transport suggesting a hydraulic barrier due to the change/inversion of the baroclinic pressure gradient,resulting in water and material *** deposition was observed during neap tide,while during spring tide the material is resuspended,increasing the concentration,generating cycles of deposition and erosion during the neap-spring *** sediment in suspension that reach the estuary,even with low fluvial volume,stay in this environment forming new islands because of *** deposition rates or sediment cycling,if generated by the hydraulic barrier,may indicate that the flo

关键词： Brazilian Amazonia Materials discharges Macrotidal estuaries Freshwater percentage Residence time

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LC–MS characterization, anti-kinetoplastide and cytotoxic activities of natural products from Eugenia jambolana Lam. and Eugenia uniflora

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Asian Pacific Journal of Tropical Biomedicine 2017年第9期7卷 836-841页

作者： Celestina Elba Sobral de Souza Ana Raquel Pereira da silva Janaina Esmeraldo Rocha Maria Celeste Vega Gomez Míriam Rolóm Cathia Coronel José Galberto Martins da costa Manoel L.C.Netto Larissa A.Rolim Henrique Douglas Melo Coutinho Laboratório de Microbiologia e Biologia Molecular Universidade Regional do Cariri Center for the Development of Scientific Research Moisés Bertoni Foundation/Díaz Gill Laboratories Laboratório de Pesquisa de Produtos Naturais Universidade Regional do Cariri Centro Universitário Dr.Leao Sampaio–UNILEAO Universidade Federal do Vale do Sao Francisco–UNIVASF

Objective: To evaluate the trypanocidal, leishmanicidal and cytotoxic activity of Eugenia jambolana(E. jambolana) and Eugenia uniflora(E. uniflora) extracts and ***: The products were characterized by LC–MS. Antiparasitic assays were performed and cytotoxicity was evaluated in fibroblastos. In vitro assays were performed using spectrophotometric evaluation. All assays were performed in ***: The results showed that the extracts and the tannic fraction from E. jambolana inhibited 100% of the epimastigote lines. The ethanolic extract was the most efficient in all concentrations tested against the three parasite strains. In the cytotoxicity assay the flavonoid fraction showed low toxicity. All E. uniflora samples showed cytotoxicity at the highest concentration tested, but the extract showed no toxic effect on the fibroblasts at the lowest concentration. The flavonoid and tannic fractions were more efficient against Leishmania braziliensis promastigotes compared to the extract. However, the extracts and the tannic fraction were more effective against Leishmania infantum strains. The effect on epimastigote cells was observed at all concentrations tested, with all E. uniflora samples. However, the samples were more effective at the highest concentration, where there was inhibition in 100% of the Trypanosoma cruzi ***: The species E. jambolana and E. uniflora presented antiparasitic activity against all tested parasite strains, indicating that these species can serve as an alternative therapy as they were efficient in the tests performed. The E. uniflora extract and the E. jambolana flavonoid fraction presented a low cytotoxicity, opening the floor for new biological studies.

关键词： Antiparasitic effect Cytotoxicity Eugenia jambolana Eugenia uniflora

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Evaluation of parasitemia by qPCR in patients with chronic Chagas disease treated with benznidazole

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Asian Pacific Journal of Tropical Medicine 2023年第12期16卷 565-572页

作者： Tycha Bianca Sabaini Pavan Eros Antônio de Almeida Rodrigo Gonçalves de Lima Jamiro da silva Wanderley Luiz Cláudio Martins Sandra Cecília Botelho costa Gláucia Elisete Barbosa Marcon Departamento de Clínica Médica Faculdade de Ciências MédicasUniversidade Estadual de Campinas(Unicamp)CampinasSão PauloBrasil Fundação Oswaldo Cruz Fiocruz Mato Grosso do SulCampo GrandeMSBrasil

Objective:To evaluate parasitemia by qPCR in patients undergoing etiological treatment and followed in a Brazilian reference ***:Parasite load was quantified by qPCR in 32 participants with chronic Chagas disease who were treated with *** analyses were performed before and after the treatment and parasite loads were compared prior and 12/18 months post the ***:Thirty-two participants were recruited and treated with benznidazole,and 20 were *** events(AE)were observed in 22 out of 29 participants that had safety data(76%),and dermatological alterations were the most frequently observed *** the 20 participants analyzed,13 and 7 completed 12 and 18 months follow-up after the treatment,respectively.12 Months after the final treatment,Trypanosoma cruzi was detectable in 3 patients by qPCR;18 months after the final treatment,Trypanosoma cruzi was detectable per qPCR in 4 of the 7 ***,between 12 and 18 months,7 participants of the 20 initial follow-up cases showed positive qPCR,indicating treatment ***:qPCR can be used as an alternative method for evaluating the effectiveness of the etiological treatment of CD,and can be applied to analyze early therapeutic *** study showed that benznidazole therapy had limited effectiveness in treating chronic CD patients,thus emphasizing the importance of conducting continued research for developing more effective therapies and diagnosis for CD.

关键词： Trypanosoma cruzi Chagas disease Antitrypanosomal therapy Benznidazole qPCR Therapeutic failure

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Two Forms of Experimental Simulation Analysis for Multiaxial Drilling Efforts in the Time Domain Model

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材料科学与工程（中英文B版） 2016年第1期6卷 1-13页

作者： Dionisio Jose Rodrigues da costa Jose Flavio Feiteira Jorge A. R. Duran Ladario da silva Department of Metallurgical and Mechanical Engineering Fluminense Federal University Volta Redonda 27255-125 Brazil Department of Mechanical Engineering Fluminense Federal University Volta Redonda 27255-125 Brazil Department of Physics Exact Sciences Institute Fluminense Federal University Volta Redonda 27213-145 Brazil

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Effects of Cryofrequency in the Different Swine Skin Tissue Layers: <i>In Vitro</i>and <i>in Vivo</i>Study

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Journal of Biosciences and Medicines 2021年第3期9卷 94-104页

作者： Rafaella Rêgo Maia Patrícia Froes Meyer Josebel da costa Emerenciano Joseela Joyce Barbosa da silva Júlio davi costa e silva Liliane Santos de Vasconcellos Rodrigo Marcel Valentim da silva Tarciso Bruno Montenegro Sampaio Ciro dantas Soares Federal Universityof Rio Grande do Norte (UFRN) Natal Brazil University Centre of Rio Grande do Norte (UNI-RN) Natal Brazil Potiguar University (UNP) Natal Brazil Maurício de Nassau College Natal Brazil Universityof Campinas (UNICAMP) S&atilde o Paulo Brazil

Introduction: Cryofrequency is a novel therapy which was developed with the combination of cold (cryotherapy) and heat (radiofrequency) application, causing a thermal shock effect on skin tissue. The thermal shock is believed to alter local metabolism. Objective: To investigate the effect of cryofrequency on the skin tissue of a mini pig in an in vitro and in vivo experimental model. Methodology: A small swine of the S. domesticus species, a male mini-pig (30 kg weight) was elected for the in vivo procedure subject. The in vitro step was performed on the extracted abdominal part of another subject of the same species and breed. Then, after the application of cryofrequency in the monopolar and bipolar form in vivo, a histological analysis was performed. Results: A higher temperature variation was observed comparing the pilot study in vitro with respect to the experimental one in vivo. Microscopically, an increase of collagen fibers and blood supply and decreased adipose tissue was observed after cryofrequency application. Conclusion: With the bipolar cryofrequency, no reduction of adipose tissue was observed, but regarding the connective tissue, extensive areas of collagen deposition were observed, as well as a great amount of new-formed collagen fibers.

关键词： Cryotherapy Radio Frequency Adiposity Collagen Temperature

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Anti-inflammatory effects ofα-humulene andβ-caryophyllene on pterygium fibroblasts

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International Journal of Ophthalmology(English edition) 2022年第12期15卷 1903-1907页

作者： Magda Massae Hata Viveiros Márcia Guimarães silva JoséGalberto Martins da costa Anselmo Gomes de Oliveira Carolina Rubio Carlos Roberto Padovani Cláudia Aparecida Rainho silvana Artioli Schellini Department of Ophthalmology School of Medicine of BotucatuSão Paulo State University–UNESPBotucatuSao Paulo 18618-000Brazil Department of Pathology School of Medicine of BotucatuSão Paulo State University–UNESPBotucatuSao Paulo 01049-010Brazil Department of Biological Chemistry Regional University of CaririCratoCeará66100-900Brazil Department of Pharmacology School of Pharmaceutical SciencesSão Paulo State University–UNESPAraraquaraSao Paulo 01049-010Brazil Biological Sciences Institute of Biosciences of BotucatuSão Paulo State University–UNESPBotucatuSao Paulo 01049-010Brazil Department of Biostatistics São Paulo State University–UNESPBotucatuSao Paulo 01049-010Brazil

AIM:To investigate the anti-inflammatory effects of the sesquiterpenesα-humulene andβ-caryophyllene on pterygium ***:Primary cultures of pterygium fibroblasts were *** passage pterygium fibroblasts were exposed toα-humulene andβ-caryophyllene separately and *** cell viability was assessed by 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide(MTT)assay at 12,24,48,and 72h after *** levels of the inflammatory cytokines interleukin(IL)-1β,IL-6,IL-8,tumor necrosis factor(TNF)-αand IL-10 in the conditioned culture medium were assessed by enzymelinked immunosorbent assay(ELISA)at 12,24 and 48h after *** were statistically analyzed using Friedman repeated measures analysis of variances on ***:The 25μmol/Lβ-caryophyllene induced significant decrease in the IL-6 production by pterygium fibroblasts 48h after the exposure(P=0.041).The levels of IL-1β,IL-8,IL-10,and TNF-αwere very low and had no statistically significant variations af ter exposure toα-humulene,β-caryophyllene,or both compounds ***:The exposure to 25μmol/L ofβ-caryophyllene significantly reduce the production of IL-6 by pterygium fibroblasts after *** sesquiterpene may be a potential alternative adjuvant agent for the treatment of pterygium.

关键词： pterygium fibroblasts β-caryophyllene α-humulene cytokines

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Non-pharmacological management of pediatric functional abdominal pain disorders:Current evidence and future perspectives

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World Journal of Clinical Pediatrics 2022年第2期11卷 105-119页

作者： Maria Luísa Cordeiro Santos Ronaldo Teixeira da silva Júnior Breno Bittencourt de Brito Filipe Antônio França da silva Hanna Santos Marques Vinícius Lima de SouzaGonçalves Talita costa dos Santos Carolina Ladeia Cirne Natália Oliveira e silva Márcio Vasconcelos Oliveira Fabrício Freire de Melo Instituto Multidisciplinar em Saúde Universidade Federal da BahiaVitória da Conquista 45029-094BahiaBrazil Campus Vitória da Conquista Universidade Estadual do Sudoeste da BahiaVitória da Conquista 45083-900BahiaBrazil

Functional abdominal pain disorders(FAPDs) are an important and prevalent cause of functional gastrointestinal disorders among children, encompassing the diagnoses of functional dyspepsia, irritable bowel syndrome, abdominal migraine, and the one not previously present in Rome Ⅲ, functional abdominal pain not otherwise specified. In the absence of sufficiently effective and safe pharmacological treatments for this public problem, non-pharmacological therapies emerge as a viable means of treating these patients, avoiding not only possible side effects, but also unnecessary prescription, since many of the pharmacological treatments prescribed do not have good efficacy when compared to placebo. Thus, the present study provides a review of current and relevant evidence on non-pharmacological management of FAPDs, covering the most commonly indicated treatments, from cognitive behavioral therapy to meditation, acupuncture, yoga, massage, spinal manipulation, moxibustion, and physical activities. In addition, this article also analyzes the quality of publications in the area, assessing whether it is possible to state if non-pharmacological therapies are viable, safe, and sufficiently well-based for an appropriate and effective prescription of these treatments. Finally, it is possible to observe an increase not only in the number of publications on the non-pharmacological treatments for FAPDs in recent years, but also an increase in the quality of these publications. Finally, the sample selection of satisfactory age groups in these studies enables the formulation of specific guidelines for this age group, thus avoiding the need for adaptation of prescriptions initially made for adults, but for children use.

关键词： Functional abdominal pain disorder Pediatrics Rome IV Behavioral intervention Nonpharmacological treatment Complementary medicine

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Relevance of MUC1 mucin variable number of tandem repeats polymorphism in H pylori adhesion to gastric epithelial cells

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World Journal of Gastroenterology 2008年第9期14卷 1411-1414页

作者： Natália R costa Nuno Mendes Nuno T Marcos Celso A Reis Thomas Caffrey Michael A Hollingsworth Filipe Santos-silva Institute of Molecular Pathology and Immunology University of PortoPorto 4200-465Portugal Institute of Molecular Pathology and Immunology University of Porto Porto 4200-465Portugal Medical Faculty University of Porto Porto 4200-465Portugal Eppley Institute for Research in Cancer and Allied Diseases University of Nebraska Medical CenterOmaha 68198-6805United States Institute of Molecular Pathology and Immunology University of PortoPorto 4200-465Portugal

AIM:To evaluate the influence of MUC1 mucin variable number of tandem repeats (VNTR) variability on H pylori adhesion to gastric cells. METHODS: Enzyme linked immunosorbent assay (ELISA)-based adhesion assays were performed to measure the adhesion of different H pylori strains (HP26695 and HPTx30a) to gastric carcinoma cell lines (GP202 and MKN45) and GP202 clones expressing recombinant MUC1 with different VNTR lengths. RESULTS: Evaluation of adhesion results shows that H pylori pathogenic strain HP26695 has a significantly higher (P < 0.05) adhesion to all the cell lines and clones tested, when compared to the non-pathogenic strain HPTx30a. Bacteria showed a significantly higher (P < 0.05) adhesion to the GP202 cell line, when compared to the MKN45 cell line. Furthermore, both strains showed a significantly higher (P < 0.05) adhesion to GP202 clones with larger MUC1 VNTR domains. CONCLUSION: This work shows that MUC1 mucin variability conditions H pylori binding to gastric cells. The extent of bacterial adhesion depends on the size of theMUC1 VNTR domain. The adhesion is further dependent on bacterial pathogenicity and the gastric cell line. MUC1 mucin variability may contribute to determine H pylori colonization of the gastric mucosa.

关键词： Hpylori MUC1 Variable number of tandem repeats Polymorphism Adhesion Mucin Gastric Infection

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Obstetricians and pregnant women from Formiga’s town, in Minas Gerais State, Brazil. Deciding what kind of labor to be held

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Open Journal of Obstetrics and Gynecology 2013年第1期3卷 90-96页

作者： Heslley Machado silva Angélica Rodrigues da costa Radassa de Avelar Nogueira Herculano Department of Itaúna’s University/MG Itaúna Brazil Department of University Center UNIFOR/MG and Itaúna’s University/MG Itaúna Brazil Department of University Center UNIFOR/MG—FAPEMIG University Center of UNIFOR/MG Formiga Brazil

The decision of what kind of labor that should be held is intermittently generating a great debate in Brazil. The growth in the number of cesarean sections has raised worries in some of the involved sectors of the society. It was proposed to investigate the factors which lead the women to take this decision, trying to understand the principle involved. A bibliographic review about the subject was made. The research happened in the town of Formiga, where some data were collected in two hospitals—public and private —about the number of labors during two months. Subsequently, the obstetricians and a group of pregnant women were submitted to a questionnaire, the answers were compiled and transformed into graphs which were analyzed. Finally, the data were compared with the related literature. The number of cesarean sections in the town was above the standard recommended. According to the obstetricians, the main reason would be the preference of the pregnant women, fact which was not confirmed by them, that discrepancy was confirmed by the literature. The level of information about the clinical indications for the type of labor was considered satisfactory by the doctors and the pregnant women, data contestable by the academics. The fear and the pain were identified by obstetricians and pregnant women as a predominant factor for the preference of cesarean sections, provided that, according to the literature, shows the precariousness of information for pregnant women about the normal birth. Another important data is that the doctors can have an important role in the kind of labor to be made. According to the obstetricians, their role would be very limited, but according to some pregnant women, they did trust in the doctors which should be the ideal labor for them. The analysis of most of the data collected shows a discrepancy between the information of pregnant women and obstetricians. Both sides involved in the issue do not admit as responsible for the elevate number of ce

关键词： Birth Public Health Pregnant Women Natural Childbirth Cesarean Section Obstetrics

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