Evaluation of parasitemia by qPCR in patients with chronic Chagas disease treated with benznidazole

作     者：Tycha Bianca Sabaini Pavan Eros Antônio de Almeida Rodrigo Gonçalves de Lima Jamiro da Silva Wanderley Luiz Cláudio Martins Sandra Cecília Botelho Costa Gláucia Elisete Barbosa Marcon Tycha Bianca Sabaini Pavan;Eros Antônio de Almeida;Rodrigo Gonçalves de Lima;Jamiro da Silva Wanderley;Luiz Cláudio Martins;Sandra Cecília Botelho Costa;Gláucia Elisete Barbosa Marcon

作者机构：Departamento de Clínica MédicaFaculdade de Ciências MédicasUniversidade Estadual de Campinas(Unicamp)CampinasSão PauloBrasil Fundação Oswaldo CruzFiocruz Mato Grosso do SulCampo GrandeMSBrasil 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2023年第16卷第12期

页      面：565-572页

核心收录：

学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Fundação de AmparoàPesquisa do Estado de São Paulo-FAPESP.Process number 2016/08737-0 TBSP received a Ph.D.scholarship from the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior(CAPES,Finance code 001) 

主　　题：Trypanosoma cruzi Chagas disease Antitrypanosomal therapy Benznidazole qPCR Therapeutic failure 

摘      要：Objective:To evaluate parasitemia by qPCR in patients undergoing etiological treatment and followed in a Brazilian reference ***:Parasite load was quantified by qPCR in 32 participants with chronic Chagas disease who were treated with *** analyses were performed before and after the treatment and parasite loads were compared prior and 12/18 months post the ***:Thirty-two participants were recruited and treated with benznidazole,and 20 were *** events(AE)were observed in 22 out of 29 participants that had safety data(76%),and dermatological alterations were the most frequently observed *** the 20 participants analyzed,13 and 7 completed 12 and 18 months follow-up after the treatment,respectively.12 Months after the final treatment,Trypanosoma cruzi was detectable in 3 patients by qPCR;18 months after the final treatment,Trypanosoma cruzi was detectable per qPCR in 4 of the 7 ***,between 12 and 18 months,7 participants of the 20 initial follow-up cases showed positive qPCR,indicating treatment ***:qPCR can be used as an alternative method for evaluating the effectiveness of the etiological treatment of CD,and can be applied to analyze early therapeutic *** study showed that benznidazole therapy had limited effectiveness in treating chronic CD patients,thus emphasizing the importance of conducting continued research for developing more effective therapies and diagnosis for CD.