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OsSCE1 Encoding SUMO E2-Conjugating Enzyme Involves in Drought Stress Response of Oryza sativa

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Rice science 2018年第2期25卷 73-81页

作者： dini NURDIANI Dwi WIDYAJAYANTIE Satya NUGROHO Laboratory of Genomics and Crop Improvement Research Center for Biotechnology Indonesian Institute of Sciences

Small ubiquitin-like modifier(SUMO)-conjugating enzymes are involved in post-translational regulatory processes in eukaryotes, including the conjugation of SUMO peptides to protein substrate(SUMOylation). SUMOylation plays an important role in improving plant tolerance to abiotic stress such as salt, drought, heat and cold. Herein, we reported the isolation of OsSCE1(LOC_Os10 g39120) gene encoding a SUMO-conjugating enzyme from rice(Oryza sativa cv. Nipponbare) and its functional validation in response to drought stress. The E2 enzyme, Os SCE1, is one of three key enzymes involved in the conjugation of SUMO to its target proteins. Activated SUMO is transferred to the cysteine of an E2 enzyme and then to the target lysine residue of the substrate, with or without the help of an E3 SUMO ligase. Expression of OsSCE1 was strongly induced by polyethylene glycol 6000(PEG6000) treatment, which suggested OsSCE1 may be involved in the drought stress response. Overexpression of OsSCE1(OsSCE1-OX) in Nipponbare reduced the tolerance to drought stress. Conversely, the drought tolerance was slightly improved by the knockdown of OsSCE1(OsSCE1-KD). These results were further supported by measurement of proline content in OsSCE1-OX and OsSCE1-KD transgenic lines under induced drought stress, which showed OsSCE1-KD transgenic lines accumulated higher proline content than the wild type, whereas OsSCE1-OX line had lower proline content than the wild type. These findings suggested OsSCE1 may play a role as a negative regulator in response to drought stress in rice.

关键词： Oryza sativa drought stress small ubiquitin-like modifier SUMO-conjugating enzyme proline content gene expression

Twenty years of population dynamics in European beech-oak forest at their rear range margin anticipate changes in its structure and composition

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Forest Ecosystems 2024年第3期11卷 358-370页

作者： álvaro Rubio-Cuadrado Iciar Alberdi Isabel canellas Fernando Montes Jesús Rodríguez-calcerrada Rosana López Guillermo G.Gordaliza María Valbuena-carabana Nikos Nanos Ramón Perea Luis Gil Departamento de dinamica y gestion forestal Instituto de Ciencias Forestales(ICIFOR-INIA)CSICCtra.La Coruna km 7.528040MadridSpain Departamento de Sistemas y Recursos Naturales Escuela Tecnica Superior de Ingeniería de MontesForestal y del Medio NaturalUniversidad Politecnica de Madrid.Ciudad Universitaria s/n28040MadridSpain School of Forestry and Natural Environment Aristotle University of ThessalonikiUniversity Campus54124ThessalonikiGreece

There is an increasing interest in restoring degraded forests,which occupy half of the forest *** the forms of restoration,passive restoration,which involves the elimination of degrading factors and the free evolution of natural dynamics by applying minimal or no management,is gaining *** dynamics is difficult to predict due to the influence of multiple interacting factors such as climatic and edaphic conditions,composition and abundance of species,and the successional character of these ***,we study the natural dynamics of a mixed forest located in central Spain,which maintained an open forest structure,due to intensive use,until grazing and cutting were banned in the *** most frequent woody species in this forest are Fagus sylvatica,Quercus petraea,Quercus pyrenaica,Ilex aquifolium,Sorbus aucuparia,Sorbus aria and Prunus avium,with contrasting shade and drought *** species are common in temperate European deciduous forest and are found here near their southern distribution limit,except for *** order to analyze forest dynamics and composition,three inventories were carried out in 1994,2005 and *** results show that,despite the Mediterranean influence,the natural dynamics of this forest has been mainly determined by different levels of shade *** the abandonment of grazing and cutting,*** expanded rapidly due to its lower shade tolerance,whereas after canopy closure and forest densification,shade-tolerant species gained ground,particularly ***,despite its lower drought and late-frost *** the current dynamics continue,*** will overtake the rest of the species,which will be relegated to sites with shallow soils and steep ***,all the multi-centennial beech trees,which are undergoing a rapid mortality and decline process,will disappear.

关键词： Forest succession Secondary forest Woodland Mixed forest Competition Recruitment

A rapid nucleic acid detection platform based on phosphorothioate-DNA and sulfur binding domain

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Synthetic and Systems Biotechnology 2023年第2期8卷 213-219页

作者： Yuting Shuai Yi Ju Yuanhang Li dini Ma Lan Jiang Jingyu Zhang Gao-Yi Tan Xueting Liu Shenlin Wang Lixin Zhang Guang Liu State Key Laboratory of Bioreactor Engineering and School of BiotechnologyEast China University of Science and Technology(ECUST)Shanghai200237China State Key Laboratory of Microbial Metabolism Joint International Research Laboratory of Metabolic&Developmental SciencesSchool of Life Sciences&BiotechnologyShanghai Jiao Tong UniversityShanghai200240China

Nucleic acid detection plays a key role in diverse diagnosis and disease *** available nucleic acid detection techniques are challenged by trade-offs among speed,simplicity,precision and ***,we described a novel method,designated SENSOR(Sulfur DNA mediated nucleic acid sensing platform),for rapid nucleic acid *** was developed from phosphorothioate(PT)-DNA and sulfur binding domain(SBD)which specifically binds double-stranded PT-modified *** utilizes PT-DNA oligo and SBD as targeting module,which is linked with split luciferase reporter to generate luminescence signal within 10 *** tested detection on synthesized nucleic acid and COVID-19 pseudovirus,achieving attomolar sensitivity combined with an amplification *** nucleotide polymorphisms(SNP)could also be *** SENSOR a new promising nucleic acid detection technique.

关键词： Phosphorothioate Sulfur binding domain Nucleic acid detection

A unified electrical model based on experimental data to describe electrical transport in carbon nanotube-based materials

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Nano Research 2020年第6期13卷 1764-1779页

作者： Yoann dini Jérôme Faure-Vincent Jean Dijon Univ.Grenoble-Alpes CEALITENDTNMF-38000GrenobleFrance Univ.Grenoble-Alpes CEACNRSIRIGSyMMESF-38000GrenobleFrance

Understanding the electrical transport in carbon nanotube(CNT)materials is one key to reach very high electrical *** CNT material resistivity(ρ(T))as function of the temperature are fully apprehended by their reduced activation W(T)=dln(ρ)/dln(T)*** to now,no model accurately fits W(T)curves,thus preventing from precisely describing the CNT material electrical *** present a new electrical transport model that perfectly fits all W(T)curves found in the literature and in our own *** material resistivities are modeled byρ(T)=ρ0(T^−α+M(1+βTγT^2)).Our model has few enough parameters(α,M,β,γ)to relate them to the CNT *** 70 K,we experimentally show that CNT material resistivity follows the Luttinger Liquid theory justifying the T^−αterm in our *** 70 K,the polynomial part becomes dominant and depends on the two different CNT fabrication techniques which lead to two very different yarn *** yarns made from floating catalyst chemical vapor deposition CNTs,the polynomial is explained by the percolation of metallic CNT ***,the polynomial of yarns spun from CNT arrays is explained by the electrical transport in CNT bundles which are the basic building blocks of this type of yarns.

关键词： carbon nanotube carbon-based materials electrical conductivity electrical transport model Luttinger Liquid

Influence of broken kernels content on soybean quality during storage

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Grain & Oil Science and Technology 2024年第2期7卷 105-112页

作者： Lazaro da Costa Correa canizares Cesar Augusto Gaioso Newiton da Silva Timm Silvia Leticia Rivero Meza Adriano Hirsch Ramos Maurício de Oliveira Everton Lutz Moacir cardoso Elias Department of Agroindustry Science and Technology Federal University of PelotasPelotasRio Grande do Sul 96010-900Brazil Information and Communication Technological Axis Federal Institute of EducationScienceand Technology FarroupilhaCampus PanambiPanambiRio Grande do Sul 98280-000Brazil

Although it is recognized that the post-harvest system is most responsible for the loss of soybean quality,the real impact of this loss is still *** regulation allows 15%and 30%of broken soybean for group I and group II(quality groups),***,the industry is not informed about the loss in the quality parameters of soybeans and its impacts during long-term ***,the objective was to evaluate the effect of the breakage kernel percentage of soybean stored for 12 *** of 15% of breakage kernels did not affect soybean ***,content of 30% of breakage kernels affected significantly soybean quality,which was evidenced by the increase of up to 75% in moldy soybeans,72% in acidity,50% in leached solids,27% in electrical conductivity,and the decrease of up to 12% in soluble protein,6.4% in germination and 3.5% in thousand kernel weight after 8 months of *** the legislation establishes a percentage limit,it is recommended to store soybeans with up to 15% breakage *** the contrary,values higher than that can cause a significant reduction in soybean quality,resulting in commercial losses.

关键词： Soybean quality Breakage kernels Storage problems Grain defects Quality parameters

Thermo-magnetic analysis of thick-walled spherical pressure vessels made of functionally graded materials

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Applied Mathematics and Mechanics(English Edition) 2019年第6期40卷 751-766页

作者： M. A. NEMATOLLAHI A. dini M. HOSSEINI Department of Biosystems Engineering College of AgricultureShiraz UniversityShiraz 71441-65186Iran Department of Mechanical Engineering Ferdowsi University of MashhadMashhad 91775-1111Iran Department of Mechanical Engineering Sirjan University of TechnologySirjan 78137-33385Iran

This study presents an analytical solution of thermal and mechanical displacements, strains, and stresses for a thick-walled rotating spherical pressure vessel made of functionally graded materials (FGMs). The pressure vessel is subject to axisymmetric mechanical and thermal loadings within a uniform magnetic field. The material properties of the FGM are considered as the power-law distribution along the thickness. Navier’s equation, which is a second-order ordinary differential equation, is derived from the mechanical equilibrium equation with the consideration of the thermal stresses and the Lorentz force resulting from the magnetic field. The distributions of the displacement, strains, and stresses are determined by the exact solution to Navier’s equation. Numerical results clarify the influence of the thermal loading, magnetic field, non-homogeneity constant, internal pressure, and angular velocity on the magneto-thermo-elastic response of the functionally graded spherical vessel. It is observed that these parameters have remarkable effects on the distributions of radial displacement, radial and circumferential strains, and radial and circumferential stresses.

关键词： analytical solution magnetic field thermal loading rotating thick-walled spherical pressure vessel functionally graded material (FGM)

Seismic assessment of nature-inspired hexagrid lateral load-resisting system

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Earthquake Engineering and Engineering Vibration 2021年第3期20卷 661-672页

作者： Yasaman Memarzadeh Kiani Seyed Hossein Hosseini Lavassani Afshin Meshkat-dini Department of Civil Engineering Faculty of EngineeringKharazmi UniversityTehranIran

This paper provides a general perspective of the seismic performance of a nature-inspired,honey-comb grid structural system,known as a hexagrid,under near-field ground *** performance of this skeleton is then compared to that of a bundled-tube,as a conventional and efficient load-resisting system in order to provide a better perception of the seismic behavior of a hexagrid *** 20-story buildings with bundled-tube and hexagrid skeleton were *** behavior of the structures was investigated through 3-D finite element computer models and nonlinear time history analyses by subjecting the models to seven three-component records of scaled near-field ground *** of peak inter story drift and corner beam-column joint rotations were calculated and *** indicated that by replacing the exterior columns of the bundled-tube system with inclined beam-column elements of nature-inspired hexagons,lateral stiffness of the building increased and it would tolerate less deformations before global dynamic instability is *** presence of inclined columns in the hexagrid skeleton helped to concentrate local nonlinearities in ring beams rather than exterior columns.

关键词： high-rise steel structure hexagrid structure performance assessment nonlinear seismic analysis

全容积IVIM直方图分析对乳腺良恶性病变的影响

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中国CT和MRI杂志 2024年第3期22卷 82-85页

作者：李俊蔡小丽吴奇川牟方胜重庆大学附属三峡医院放射科重庆404000

目的探讨全容积测量体素内不相干运动(IVIM)直方图参数对乳腺良恶性病变的诊断价值。方法收集我院2021年2月-2022年10月术前行多模态磁共振检查并获得病理的153例女性共158例肿瘤。利用直方图处理软件得到肿瘤IVIM(D、D*、f)的直方图... 详细信息

目的探讨全容积测量体素内不相干运动(IVIM)直方图参数对乳腺良恶性病变的诊断价值。方法收集我院2021年2月-2022年10月术前行多模态磁共振检查并获得病理的153例女性共158例肿瘤。利用直方图处理软件得到肿瘤IVIM(D、D*、f)的直方图参数。采用两独立样本t检验(正态分布)或Mann-WhitneyU检验(偏态分布)比较乳腺良、恶性病灶的IVIM直方图参数的差异。绘制ROC评价IVIM直方图参数独立和联合鉴别诊断乳腺良、恶性病变的效能,Z检验评价联合诊断ROC下面积(AUC)差异性。单变量及多变量二元logisti回归分析独立影响因素。结果乳腺恶性病变116个,良性病变42个。良、恶性病变D(90th、熵、峰度、均值、中位数、最小值、偏度)、f峰度、D*(90th、熵、峰度、均值、中位数、最大值、偏度)单因素分析有统计学意义。AUC最大为D偏度(0.818);敏感度最高为D熵(0.957);特异度最高为D偏度(0.857);准确度最高为D偏度(0.797)。D直方图联合诊断AUC、敏感度、特异度、准确度分别为0.903、0.948、0.786、0.88;f直方图联合诊断分别为0.604、0.672、0.548、0.73;D*直方图联合诊断为0.833、0.897、0.619、0.823;IVIM直方图联合诊断为0.953、0.982、0.833、0.924;IVIM直方图联合诊断AUC面积最大,与D值、f值、D*直方图分别联合诊断AUC差异有统计学意义。D熵(OR=2.943;P=0.037)及D中位数(OR=0.984;P=0.029)为乳腺恶性病变独立影响因素。结论乳腺磁共振IVIM直方图参数联合能显著提高判断乳腺良恶性病变诊断效能,D熵及D中位数为乳腺恶性病变独立影响因素。

关键词：乳腺病变体素内不相干运动成像联合诊断影响因素

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Three-dimensional mixed convection stagnation-point fow past a vertical surface with second-order slip velocity

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Applied Mathematics and Mechanics(English Edition) 2023年第4期44卷 641-652页

作者： A.V.ROSca N.C.ROSca I.POP Department of Statistics-Forecasts-Mathematics Faculty of Economics and Business AdministrationBabes-Bolyai UniversityCluj-Napoca 400084Romania Department of Mathematics Faculty of Mathematics and Computer ScienceBabes-Bolyai UniversityCluj-Napoca 400084Romania Academy of Romanian Scientists 3 Ilfov StreetBucharest 050044Romania

This study is concerned with the three-dimensional(3D)stagnation-point for the mixed convection flow past a vertical surface considering the first-order and secondorder velocity *** the authors’knowledge,this is the first study presenting this very interesting *** partial differential equations for the flow problem are transformed into nonlinear ordinary differential equations(ODEs)by using appropriate similarity *** ODEs with the corresponding boundary conditions are numerically solved by utilizing the bvp4c solver in MATLAB programming *** effects of the governing parameters on the non-dimensional velocity profiles,temperature profiles,skin friction coefficients,and the local Nusselt number are presented in detail through a series of graphs and ***,it is reported that the reduced skin friction coefficient decreases for the assisting flow situation and increases for the opposing flow *** numerical computations of the present work are compared with those from other research available in specific situations,and an excellent consensus is *** exciting feature for this work is the existence of dual *** important remark is that the dual solutions exist for both assisting and opposing flows.A linear stability analysis is performed showing that one solution is stable and the other solution is not *** notice that the mixed convection and velocity slip parameters have strong effects on the flow *** effects are depicted in graphs and discussed in this *** obtained results show that the first-order and second-order slip parameters have a considerable effect on the flow,as well as on the heat transfer characteristics.

关键词： three-dimensional(3D)mixed convection flow stagnation point flow first-order slip velocity second-order slip velocity numerical solution stability analysis