A rapid nucleic acid detection platform based on phosphorothioate-DNA and sulfur binding domain

作     者：Yuting Shuai Yi Ju Yuanhang Li Dini Ma Lan Jiang Jingyu Zhang Gao-Yi Tan Xueting Liu Shenlin Wang Lixin Zhang Guang Liu 

作者机构：State Key Laboratory of Bioreactor Engineeringand School of BiotechnologyEast China University of Science and Technology(ECUST)Shanghai200237China State Key Laboratory of Microbial MetabolismJoint International Research Laboratory of Metabolic&Developmental SciencesSchool of Life Sciences&BiotechnologyShanghai Jiao Tong UniversityShanghai200240China 

出 版 物：《Synthetic and Systems Biotechnology》 (合成和系统生物技术（英文）)

年 卷 期：2023年第8卷第2期

页      面：213-219页

核心收录：

学科分类：071011[理学-生物物理学] 0710[理学-生物学] 07[理学] 

基　　金：supported by the National Natural Science Foundation of China(31900060) National Key Research and Development Program of China(2020YFA0907800,2022YFC3400200,2022YFA0912200) Natural Science Foundation of Shanghai(20ZR1414500) Shanghai Pilot Program for Basic Research-Shanghai Jiao Tong University(21TQ1400204) 

主　　题：Phosphorothioate Sulfur binding domain Nucleic acid detection 

摘      要：Nucleic acid detection plays a key role in diverse diagnosis and disease *** available nucleic acid detection techniques are challenged by trade-offs among speed,simplicity,precision and ***,we described a novel method,designated SENSOR(Sulfur DNA mediated nucleic acid sensing platform),for rapid nucleic acid *** was developed from phosphorothioate(PT)-DNA and sulfur binding domain(SBD)which specifically binds double-stranded PT-modified *** utilizes PT-DNA oligo and SBD as targeting module,which is linked with split luciferase reporter to generate luminescence signal within 10 *** tested detection on synthesized nucleic acid and COVID-19 pseudovirus,achieving attomolar sensitivity combined with an amplification *** nucleotide polymorphisms(SNP)could also be *** SENSOR a new promising nucleic acid detection technique.