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Science China(Life Sciences) 2021年第9期64卷 1522-1532页

作者： Xiao-Feng Zhang Tianbao Zeng Yunjie Xie Yuemin Zheng Huanqin wang Hanbin Lin zongwen wang Taiyun Wei State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops Institute of Plant VirologyFujian Agriculture and Forestry UniversityFuzhou 350002China

Intracellular polyamines(putrescine,spermidine,and spermine)have emerged as important molecules for viral infection;however,how viruses activate polyamines biosynthesis to promote viral infection remains *** decarboxylase 1(ODC1)and its antienzyme 1(OAZ1)are major regulators of polyamine biosynthesis in animal ***,we report that rice yellow stunt virus(RYSV),a plant rhabdovirus,could activate putrescine biosynthesis in leafhoppers to promote viral propagation by inhibiting OAZ1 *** observed that the reduction of putrescine biosynthesis by treatment with difluormethylornithine(DFMO),a specific nontoxic inhibitor of ODC1,or with in vitro synthesized dsRNAs targeting ODC1 mRNA could inhibit viral *** contrast,the supplement of putrescine or the increase of putrescine biosynthesis by treatment with ds RNAs targeting OAZ1 mRNA could facilitate viral *** further determined that both RYSV matrix protein M and ODC1 directly bind to the ODC-binding domain at the C-terminus of ***,viral propagation in leafhoppers would decrease the ability of OAZ1 to target and mediate the degradation of ODC1,which finally activates putrescine production to benefit viral *** work reveals that polyamine-metabolizing enzymes are directly exploited by a vector-borne virus to increase polyamine production,thereby facilitating viral infection in insect vectors.

关键词： rice yellow stunt virus leafhopper polyamines viral infection plant virus

Neuro-protective effects of CNTF on hippocampal neurons via an unknown signal transduction pathway

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Chinese Science Bulletin 2006年第1期51卷 48-53页

作者： LI Ping wang zongwen YAN Jin Li Zhaoshen JIANG Chunlei NI Xin YANG Yongji LIU Fang LU Changlin Department of Psychology Second Military Medical University Shanghai 200433 China Changhai Hospital Second Military Medical University Shanghai 200433 China Laboratory of Stress Medicine Second Military Medical University Shanghai 200433 China School of Basic Medicine Second Military Medical University Shanghai 200433China

In our previous study, we proposed that there may be an unknown pathway in the upper stream of the known signal transduction pathway of Ciliary neurotrophic factor (CNTF) that mediates the neuro-protective function of CNTF. In the present experiment, we observed that the neuro-protective function of the non-classic signal transduction path- way in a L-NMDA (a glutamic acid ion type receptor atagonist) induced hippocampal neuron injury model, using primary culture rat hippocampal neurons, con- tinuous photography and gp130 immunohistochemi- cal assay. The results showed that L-NMDA induced injurious reaction of hippocampal neurons, and CNTF was able to inhibit the toxic action of L-NMDA on hippocampal neurons. Additionally, when JAK/STATs in the known classic signal transduction pathway of CNTF were blocked by PTPi-2, the protective effect of CNTF against L-NMDA injury still existed. L-NMDA caused a rapid increase in the concentration of hip- pocampal intracellular free [Ca2+]i. CNTF was able to attenuate L-NMDA-induced elevation of [Ca2+]i, and blocking JAK/STATs in the known classic signal trans- duction pathway of CNTF did not affect L-NMDA- induced elevation of [Ca2+]i, indicating that, apart from the known classic signal transduction pathway, there may be some other transduction pathways for CNTF to exert the protective effect on hippocampal neurons, and this pathway is related to [Ca2+].

关键词： CNTF L-NMDA 海马神经元 JAK/STATs PTPi-2 NMDA

Polyamine-metabolizing enzymes are activated to promote the proper assembly of rice stripe mosaic virus in insect vectors

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Stress Biology 2022年第1期2卷 566-579页

作者： Dongsheng Jia Huan Liu Jian Zhang Wenqiang Wan zongwen wang Xiaofeng Zhang Qian Chen Taiyun Wei Fujian Province Key Laboratory of Plant Virology State Key Laboratory of Ecological Pest Control for Fujian and Taiwan CropsFujian Agriculture and Forestry UniversityFuzhouFujianPeople’s Republic of China

Both viruses and host cells compete for intracellular polyamines for efficient ***,how the key polyamine-metabolizing enzymes,including ornithine decarboxylase 1(ODC1)and its antizyme 1(OAZ1),are activated to co-ordinate viral propagation and polyamine biosynthesis remains ***,we report that the matrix protein of rice stripe mosaic virus(RSMV),a cytorhabdovirus,directly hijacks OAZ1 to ensure the proper assembly of rigid bacilliform non-enveloped virions in leafhopper *** matrix protein effectively competes with ODC1 to bind to OAZ1,and thus,the ability of OAZ1 to target and mediate the degradation of ODC1 is significantly inhibited during viral propagation,which finally promotes polyamines ***,OAZ1 and ODC1 are activated to synergistically promote viral persistent propagation and polyamine biosynthesis in viruliferous *** data suggest that it is a novel mechanism for rhabdovirus to exploit OAZ1 for facilitating viral assembly.

关键词： Rice stripe mosaic virus Rhabdovirus Insect vector Polyamines OAZ1 ODC1 Viral assembly

Glycolysis Enhanced and Mitochondrial Impairment in Childhood type 1 Narcolepsy

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Glycolysis Enhanced and Mitochondrial Impairment in Childhoo...

中国睡眠研究会第十五届全国学术年会

作者： Huijuan Wu zongwen wang Zhongxin Zhao Second Affiliated Hospital of Naval Medical University

Objective: Obesity is common in childhood narcolepsy type 1(NT1), but the mechanism is still *** study aims to determine the characteristics of the metabolic dysfunction in NT1 and to reveal the underline ***: A total of 82 Chinese patients diagnosed with NT1 were compared with 62 age, sex, and body mass index(BMI) matched healthy *** lactate before and after aerobic exercise, lactate dehydrogenase(LDH) and its isozymes were *** performed muscle biopsies on 6 NT1 patients and 5 controls by transmission electron microscopic(TEM) to investigate mitochondrial impairment in skeletal muscle ***: Compared to the controls, patients with NT1 showed significantly higher lactate levels at baseline and after exercise, accompanied with higher levels of total LDH, LDH3, LDH4 and ***-exercise lactate was demonstrated the best performance in differentiating NT1 with healthy *** investigations discover morphological mitochondria impairment, accumulation of glycogen granules, and lipidosis in NT1 ***: Increased pre-/post-exercise lactate indicates serial glycose and oxygen metabolic dysfunction in *** impairment of muscular cells produces lactate in serum and might be the mechanism of metabolic *** findings provide important information about the mechanisms underlying the major narcoleptic comorbidities in patients such as obesity and even lower activity.

关键词：

Therapeutic Potential of FGF21 in Alzheimer’s Disease

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Yangtze Medicine 2018年第1期2卷 1-17页

作者： Anbang Sun Benke Xu Xianwang wang Lian Liu Yun He zongwen wang Yuncai Chen Department of Anatomy Medical School of Yangtze University Jingzhou China The First Affiliated Hospital & The First School of Clinical Medicine Yangtze University Jingzhou China Department of Medical Laboratory Science Medical School of Yangtze University Jingzhou China Department of Medical Function School of Medicine Yangtze University Jingzhou China Department of Pediatrics University of California Irvine CA USA

Alzheimer’s disease (AD) is the most common form of dementia which mostly affects persons younger than 65 years old. Mounting findings showed that amyloid-β(Aβ) peptides, oxidative stress, neuroinflammation and insulin resistance may play central role in the pathogenesis of AD. There are very many methods to slow it through affecting these aforementioned factors. However, more efficient prevention of the progression of AD is still ambiguous. Fibroblast growth factor 21 (FGF21) is an endocrine hormone that is expressed by several organs. It increases insulin sensitivity and regulates lipid metabolism and energy homeostasis. Emerging evidence demonstrates that FGF21 has potential effects in the brain involving metabolic regulation, neuroprotection and cognition. Hence, we hypothesize that FGF21 may be a protective factor in AD by attenuating Aβ generation, inflammation, oxidative stress, and insulin resistance. Our hypothesis will shed new light on the understanding of pathogenesis of AD and help to find a new way to prevent the genesis and progress of AD.

关键词： FGF21 Alzheimer’ Disease Amyloid-β (Aβ) Oxidative Stress Inflamma-tion Insulin Resistance

强夯法在文莱达摩拉岛软弱夹层地基处理中的应用与研究

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施工技术 2016年第1期45卷 56-58,66页

作者：王宗文化学工业岩土工程有限公司江苏南京210044

软弱夹层的性质对地基基础形式的设计影响很大。通过强夯法在处理大面积软弱夹层地基中的应用,对工程设计参数、施工过程控制以及施工效果的检测结果进行分析,综合评价强夯法在文莱达摩拉岛的适用性,为在文莱类似工程建设提供借鉴。

关键词：地基强夯软弱夹层吹填砂承载力

基于数值模拟的顶板围岩损伤过程动力响应信号的能量分析

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岩石力学与工程学报 2009年第A1期28卷 3137-3145页

作者：曹野宋波潘建仕汪宗文北京科技大学土木与环境工程学院北京100083 招金矿业股份有限公司山东烟台265400

通过室内相似模型试验,模拟金属矿山巷道顶板从稳定状态到损伤发展直至顶板冒落的过程,利用封装应变传感器的光纤光栅锚杆监测振动信号,记录不同围压、不同冲击振动强度下裂隙顶板与完整顶板响应信号,利用小波变换提取信号的频带能量分... 详细信息

通过室内相似模型试验,模拟金属矿山巷道顶板从稳定状态到损伤发展直至顶板冒落的过程,利用封装应变传感器的光纤光栅锚杆监测振动信号,记录不同围压、不同冲击振动强度下裂隙顶板与完整顶板响应信号,利用小波变换提取信号的频带能量分布特征。根据室内试验对光纤光栅用于顶板稳定性监测的研究成果,利用径向基函数神经网络对监测时间序列进行预测,并基于频带能量的观点,利用Matlab建立不同损伤条件下的瞬态冲击信号评估系统,并对一段连续信号进行分析。损伤顶板振动响应信号的频带能量受到裂隙开展的影响,与完整顶板相比,出现明显的频带尖点,能量峰值从高频向低频移动。基于小波频带能量的围岩顶板稳定分析方法,首先,利用小波分解各频带能量分布特征,并通过分解结果中应力波的能量衰减程度可判别出岩体中是否存在裂隙;其次,利用小波分解后各频带能量比和归一化能量比来确定损伤程度和裂隙开展状态,为金属矿山巷道顶板稳定性监测提供了一种较为可靠的方法。

关键词：采矿工程爆破效应光纤光栅围岩损伤小波分析模型试验

Fluctuation of Primary Motor Cortex Excitability during Cataplexy in Narcolepsy

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Fluctuation of Primary Motor Cortex Excitability during Cata...

第八届中国睡眠医学论坛暨中国睡眠研究会睡眠障碍专业委员会学术年会

作者： Bei Huang Zhenying Qian Jihui Zhang zongwen wang Kun Chen Jijun wang Zhongxin Zhao Huijuan Wu Department of Neurology Changzheng HospitalNaval Medical University of Chinese PLA Department of EEG Source Imaging Shanghai Mental Health Center Department of Psychiatry Faculty of MedicineThe Chinese University of Hong Kong

Objective:Cataplexy is a complicated and dynamic process in narcolepsy type 1(NT1).This study aimed to clarify distinct stages during cataplectic attack and elucidate the changes of primary motor cortex(PMC) excitability during these ***:Thirty-two patients with NT1 and 29 healthy controls were recruited into this *** stages were distinguished from cataplectic attack by video-polysomnogram *** magnetic stimulation motor-evoked potential(TMS-MEP) was performed to measure the excitability of PMC during quiet wakefulness,laughter without cataplexy,and each cataplectic ***:Based on video and electromyogram observations,cataplectic attack process was divided into 4 stages:triggering(CA1),resisting(CA2),atonic(CA3),and recovering stages(CA4).Compared with healthy controls,NT1 patients showed significant decreased intracortical facilitation during quiet *** laughter stage,both patients and controls showed increased magnitude of MEP amplitude compared with quiet *** magnitude of MEP amplitude significantly increased even higher in CA1&2,and then dramatically decreased in CA3 accompanying with prolonged MEP latency compared with laughter stage and quiet *** amplitude and latency gradually recovered during ***:This study demonstrates four stages during cataplectic attack,and reveals the existence of resisting stage which might change the process of *** fluctuation of MEP amplitude and the prolonged MEP latency during cataplexy exhibit that PMC and motor control pathway participate in cataplectic *** increased MEP amplitude during CA1&2 strongly implies a compensatory mechanism in motor control to resist or avoid cataplectic attack.

关键词： narcolepsy cataplexy primary motor cortex transcranial magnetic stimulation motor control

Fatigue in Narcolepsy:associated with Serum Lactate/Lactate dehydrogenase,sleep disturbance and depression

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Fatigue in Narcolepsy:associated with Serum Lactate/Lactate ...

第八届中国睡眠医学论坛暨中国睡眠研究会睡眠障碍专业委员会学术年会

作者： zongwen wang Huijuan Wu Jianhua Zhuang Linli Qiu Xing Xu Yan wang Zhengqing Zhao Zhongxin Zhao Department of Neurology Changzheng HospitalSecond Military Medical University School of Sports and Health East China Normal University Department of Pulmonary Medicine Beijing University People’s Hospital

Aims:This study aims to assess the severity of both physical and mental fatigue in patients with narcolepsy,and explore the correlation between fatigue and lactate/lactate dehydrogenase,sleep disturbance and ***:59 patients with type 1 narcolepsy(NT1),32 patients with type 2 narcolepsy(NT2) and 37 healthy controls were *** fatigue scale(CFS-14) was used to assess *** polysomnogram and multiple sleep latency tests(MSLT) were performed in patients and *** sleepiness scale(ESS),hamilton rating scale for depression(HAM-D),serum lactate,lactate dehydrogenase(LDH) and its isozymes were also measured in all ***:75.8% patients show significant fatigue,including physical fatigue and mental *** differences are found in total fatigue,in physical fatigue and in mental *** levels of serum lactate,total LDH,LDH3,LDH4 and LDH5 are detected in narcoleptic patients than in controls.20% patients show HAM-D>7,while no control subject shows *** score showed positive correlation with BMI,LDH,LDH3,LDH4 and LDH5,but was not associated with ESS,total sleep time,and ***:High percentage patients with narcolepsy have obvious fatigue,especially physical *** severity of fatigue showed positive correlations with lactate,total LDH and LDH4,*** metabolic disorders may be the important indicators for fatigue in narcoleptic patients,while daytime sleepiness,disturbance of nocturnal sleep and depression show no significant correlation with fatigue.

关键词： Narcolepsy Fatigue Lactate sleep disturbance depression

Glycolysis Enhanced and Mitochondrial Impairment in Childhood type 1 Narcolepsy

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第八届中国睡眠医学论坛暨中国睡眠研究会睡眠障碍专业委员...

作者： Huijuan Wu zongwen wang Jihui Zhang Jianhua Zhuang Zhang Yu Jian-Sheng Lin Zhongxin Zhao Changzheng Hospital Department of Neurology the Chinese University of Hong Kong Faculty of MedicineDepartment of Psychiatry Fudan University School of Basic Medical SciencesElectron Microscopy Core Laboratory Centre de Recherche en Neurosciences de Lyon Universite Claude-Bernard Lyon

Objective:Obesity is common in childhood narcolepsy type 1（NT1）,but the mechanism is still *** study aims to determine the characteristics of the metabolic dysfunction in NT1 and to reveal the underline ***:A total of 82 Chinese patients diagnosed with NT1 were compared with 62 age,sex,and body mass index（BMI） matched healthy *** lactate before and after aerobic exercise,lactate dehydrogenase（LDH） and its isozymes were *** performed muscle biopsies on 6 NT1 patients and 5 controls by transmission electron microscopic（TEM） to investigate mitochondrial impairment in skeletal muscle ***:Compared to the controls,patients with NT1 showed significantly higher lactate levels at baseline and after exercise,accompanied with higher levels of total LDH,LDH3,LDH4 and ***-exercise lactate was demonstrated the best performance in differentiating NT1 with healthy *** investigations discover morphological mitochondria impairment,accumulation of glycogen granules,and lipidosis in NT1 ***:Increased pre-/post-exercise lactate indicates serial glycose and oxygen metabolic dysfunction in *** impairment of muscular cells produces lactate in serum and might be the mechanism of metabolic *** findings provide important information about the mechanisms underlying the major narcoleptic comorbidities in patients such as obesity and even lower activity.

关键词： narcolepsy type 1 childhood metabolism lactate mitochondria obesity