Rice yellow stunt virus activates polyamine biosynthesis to promote viral propagation in insect vectors by disrupting ornithine decarboxylase antienzyme function

作     者：Xiao-Feng Zhang Tianbao Zeng Yunjie Xie Yuemin Zheng Huanqin Wang Hanbin Lin Zongwen Wang Taiyun Wei Xiao-Feng Zhang;Tianbao Zeng;Yunjie Xie;Yuemin Zheng;Huanqin Wang;Hanbin Lin;Zongwen Wang;Taiyun Wei

作者机构：State Key Laboratory of Ecological Pest Control for Fujian and Taiwan CropsInstitute of Plant VirologyFujian Agriculture and Forestry UniversityFuzhou 350002China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2021年第64卷第9期

页      面：1522-1532页

核心收录：

学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 

基　　金：supported by the National Natural Science Foundation of China(31871931) the Fujian Agriculture and Forestry University Outstanding Young Scientists Project(xjq201705) National Key Research and Development Project of China(2018YFD0200306) 

主　　题：rice yellow stunt virus leafhopper polyamines viral infection plant virus 

摘      要：Intracellular polyamines(putrescine,spermidine,and spermine)have emerged as important molecules for viral infection;however,how viruses activate polyamines biosynthesis to promote viral infection remains *** decarboxylase 1(ODC1)and its antienzyme 1(OAZ1)are major regulators of polyamine biosynthesis in animal ***,we report that rice yellow stunt virus(RYSV),a plant rhabdovirus,could activate putrescine biosynthesis in leafhoppers to promote viral propagation by inhibiting OAZ1 *** observed that the reduction of putrescine biosynthesis by treatment with difluormethylornithine(DFMO),a specific nontoxic inhibitor of ODC1,or with in vitro synthesized dsRNAs targeting ODC1 mRNA could inhibit viral *** contrast,the supplement of putrescine or the increase of putrescine biosynthesis by treatment with ds RNAs targeting OAZ1 mRNA could facilitate viral *** further determined that both RYSV matrix protein M and ODC1 directly bind to the ODC-binding domain at the C-terminus of ***,viral propagation in leafhoppers would decrease the ability of OAZ1 to target and mediate the degradation of ODC1,which finally activates putrescine production to benefit viral *** work reveals that polyamine-metabolizing enzymes are directly exploited by a vector-borne virus to increase polyamine production,thereby facilitating viral infection in insect vectors.