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Chinese Medical Journal 2014年第10期127卷 1891-1896页

作者： Yang Yingchi Chen Hui Wang Dong Luo Wei zhu biyun Zhang Zhongtao Department of General Surgery Beijing Friendship Hospital Capital Medical University Beijing 100050 China Institute of Biomedical Engineering Capital Medical UniversityBeijing 100069 China

Background Artificial neural network （ANN） has demonstrated the ability to assimilate information from multiple sources to enable the detection of subtle and complex *** this reseamh,we evaluated an ANN model in the diagnosis of pancreatic cancer using multiple serum *** In this retrospective analysis,913 serum specimens collected at the Department of General Surgery of Beijing Friendship Hospital were analyzed for carbohydrate antigen 19-9 （CA19-9）,carbohydrate antigen 125 （CA125）,and caminoembryonic antigen （CEA）.The three tumor marker values were used as inputs into an ANN and randomized into a training set of 658 （70.31％ were malignant） and a test set of the remaining 255 samples （70.69％ were malignant）.The samples were also evaluated using a Logistic regression （LR） *** The ANN-derived composite index was superior to each of the serum tumor markers alone and the Logistic regression *** areas under receiver operating characteristic curves （AUROC） was 0.905 （95％ confidence Interval （CI） 0.868-0.942） for ANN,0.812 （95％ CI 0.762-0.863） for the Logistic regression model,0.845 （95％ CI 0.798-0.893）for CA19-9,0.795 （95％ CI 0.738-0.851） for CA125,and 0.800 （95％ Cl 0.746-0.854） for *** analysis of multiple markers yielded a high level of diagnostic accuracy （83.53％） compared to LR （74.90％）.Conclusion The performance of ANN model in the diagnosis of pancreatic cancer is better than the single tumor marker and LR model.

关键词： artificial neural network tumor markers pancreatic cancer Logistic regression

Arabidopsis ENOR3 regulates RNAi-mediated antiviral defense

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Journal of Genetics and Genomics 2018年第1期45卷 33-40页

作者： Hua Gao Mai Yang Haitao Yang Yue Qin biyun zhu Gang Xu Chengyuan Xie Dewei Wu Xiaolin Zhang Wanxiang Li Jianbin Yan Susheng Song Tiancong Qi Shou-Wei Ding Daoxin Xie Tsinghua-Peking Joint Center for Life Sciences and MOE Key Laboratory of Bioinformatics School of Life Sciences Tsinghua University Beijing 100084 China Department of Microbiology and Plant Pathology University of California Riverside CA 92521 USA Beijing Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement College of Life Sciences Capital Normal University Beijing 100048 China

Viruses can infect host plants to cause severe diseases and substantial agricultural loss, while plants have evolved RNA interference （RNAi） strategy to defend against viral infection. Despite enormous efforts, only a few host proteins in RNAi pathway were shown to mediate antiviral defense, including RNA-dependent RNA polymerase I （RDRI）, RDR6, DICER-LIKE 2 （DCL2） and DCL4. In this study, we carried out a genetic screen for antiviral factors of RNAi pathway in Arabidopsis rdr6 background via inoculation with a 2b- deficient Cucumber Mosaic Virus （CMV-△2b）. We identified a mutant susceptible to CMV-△2h, referred to as enhancer o ojrdr6 （enor） 3-1 rdr6, and found that ENOR3 encodes a functionally unknown protein with high homology to the mammalian Non Imprinted in Prader-Willi/Angelman （NIPA） magnesium transporters. ENOR3 inhibits accumulation of CMV-△2b and acts additively with RDR1, RDR6, DCL2 and DCL4 in antivira/ defense. These results uncover that ENOR3 is a key component in antiviral RNAi Dathwav, and provide new insights into antiviral immunity.

关键词： Antiviral defense CMV-△2b DCL2/4 ENOR3 RDRI/6 RNAi

Multi-Effect Evaporation Coupled with MVR Heat Pump Thermal Integration Distillation for Separating Salt Containing Methanol Wastewater

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Energy and Power Engineering 2017年第12期9卷 772-785页

作者： Deming Yang Yifan Yin Zhengguang Wang biyun zhu Qiang Gu College of Petrochemical Engineering Changzhou University Changzhou China

Due to the high energy consumption for separation of salt containing methanol wastewater, in this work, the multi-effect evaporation coupled with mechanical vapor recompression (MVR) heat pump and thermal integration technologies were raised for the first time. The ELECNRTL thermodynamic model is used to simulate and optimize the evaporation rectification process. Energy consumption and total annual cost (TAC) are taken as objective functions. The results show that multi-effect evaporation coupled with conventional distillation process can save energy consumption and TAC by 44.12% and 39.14%. The multi-effect evaporation coupled with distillation process based on MVR heat pump technology can save energy consumption and TAC by 55.27% and 47.49%, which is super to three-effect evaporation coupled with conventional distillation process. The three-effect evaporation coupled with MVR heat integration process can save energy consumption and TAC by 81.32% and 58.55%, which is more economical than other processes. It can be clearly seen that three-effect evaporation coupled with MVR heat integration process is more competitive to deal with the salt containing methanol wastewater.

关键词： Salt Containing Methanol Wastewater MVR Heat Pump Heat Integration Energy Saving

Increasing targeting scope of adenosine base editors in mouse and rat embryos through fusion of TadA deaminase with Cas9 variants

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Protein & Cell 2018年第9期9卷 814-819页

作者： Lei Yang Xiaohui Zhang Liren Wang Shuming Yin biyun zhu Ling Xie Qiuhui Duan Huiqiong Hu Rui Zheng Yu Wei Liangyue Peng Honghui Han Jiqin Zhang Wenjuan Qiu Hongquan Geng Stefan Siwko Xueli Zhang Mingyao Liu Dali Li East China Normal University and Shanghai Fengxian DistrictCentral Hospital Joint Center for Translational Medicine ShanghaiKey Laboratory of Regulatory Biology School of Life SciencesEast China Normal University Shanghai 200241 China Fengxian Hospital Affiliated to Southern Medical University Shanghai 201499 China Xinhua Hospital Shanghai Jiao Tong University School ofMedicine Shanghai 200092 China School of Life Sciences Hunan Normal University Changsha 410081 China Bioray Laboratories Inc. Shanghai 200241 China Department of Molecular and Cellular Medicine The Institute of Biosciences and Technology Texas AM University Health Science Center Houston TX 77030 USA

Dear Editor, The clustered regularly interspaced short palindromic repeat （CRISPR） system has been widely adapted to genome editing to either introduce or correct genetic mutations （Wang et al., 2016）. However, due to competition with the dominant non-homologous end-joining （NHEJ） pathway, precise genome modifications through Cas9-stimulated homologous recombination （HR） is inefficient. Through fusion of cytidine deaminases, APOBEC1 （apolipoprotein B editing complex 1） or AID （activation-induced deaminase）, with Cas9 variants, several groups have developed the cytidine base editor （BE） systems （Komor et al., 2016; Li et al., 2018; Nishida et al., 2016）. The BE system achieves programmable conversion of C-G base pairs to T.A without double-stranded DNA cleavage （Zhou et al., 2017）. More recently, adenine base editors （ABEs）, which efficiently convert A-T base pairs to G-C in genomic DNA, have been developed via fusion of an engineered tRNA adenosine deaminase （ecTadA from Escherichia coh） with nCas9 （Gaudelli et al., 2017）. The ABE system has quickly been adapted to generate disease models and correction of genetic disease in mice （Ryu et al., 2017; Liu et al, 2018）. However, whether the editing efficiency and the targeting scope of ABE could be improved is largely unexplored. In this study, we describe the efficient generation of base-edi- ted mice and rats modeling human diseases through ABEs with highest efficiency up to 100%. We also demonstrate an increase of ABE activity through injection of chemically modified tracrRNA and crRNA in mouse zygotes, and the expansion of editing scope by fusion of an ecTadA mutant to SaCas9n-KKH and Casgn-VQR variants in both cells and embryos. Our study suggests that the ABE system is a powerful and convenient tool to introduce precise base conversions in rodents.

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面向Y一代的高校图书馆文化建设研究

科技情报开发与经济 2014年第23期24卷 77-79页

作者：朱必云鄂尔多斯职业学院图书馆内蒙古鄂尔多斯017004

通过对Y一代学习特点、文化需求特点以及独特思维方式的分析,阐述了图书馆文化建设的指导思想,提出了图书馆文化建设的对策。

关键词： Y一代图书馆文化建设指导思想

分析耳穴贴压技术对胃癌患者心理状态及睡眠质量的影响

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世界睡眠医学杂志 2024年第4期11卷 762-764,768页

作者：朱碧云福建医科大学肿瘤临床医学院/福建省肿瘤医院福州350014

目的:分析耳穴贴压技术对胃癌患者心理状态及睡眠质量的影响。方法:选取2022年1月至2023年1月就诊于福建省肿瘤医院的胃癌患者80例作为研究对象,按照随机数字表法分为对照组和观察组,每组40例。对照组患者给予一般性护理干预,观察组患... 详细信息

目的:分析耳穴贴压技术对胃癌患者心理状态及睡眠质量的影响。方法:选取2022年1月至2023年1月就诊于福建省肿瘤医院的胃癌患者80例作为研究对象,按照随机数字表法分为对照组和观察组,每组40例。对照组患者给予一般性护理干预,观察组患者在对照组基础上给予耳穴贴压护理干预。采用匹兹堡睡眠质量指数(PSQI)比较2组患者睡眠质量的差异,采用行为检查表(CBCL)判定2组患者的心理状态。结果:干预后,观察组CBCL量表中的焦虑、退缩、抑郁状态评分均显著低于对照组,观察组PSQI评分显著低于对照组,差异有统计学意义(P<0.05)。结论:在常规干预基础上加用耳穴贴压技术能有效改善胃癌患者心理状态,提高患者睡眠质量,值得临床推广应用。

关键词：耳穴贴压胃癌心理状态睡眠质量行为检查

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维生素U含量测定方法的研究进展

食品工业 2020年第8期41卷 227-230页

作者：朱碧云秦博文马霞上海应用技术大学香料香精技术与工程学院上海201418 上海禾文农业科技有限公司上海201107

维生素U作为一种天然氨基酸,其化学性质为一种类似维生素的物质——S-甲基甲硫氨酸。主要从滴定法、色谱法和质谱法等不同检测方法进行综述,探讨这些分析方法的基本原理和优缺点,并对食品和药品中维生素U测定技术和方法的发展趋势进行... 详细信息

维生素U作为一种天然氨基酸,其化学性质为一种类似维生素的物质——S-甲基甲硫氨酸。主要从滴定法、色谱法和质谱法等不同检测方法进行综述,探讨这些分析方法的基本原理和优缺点,并对食品和药品中维生素U测定技术和方法的发展趋势进行展望。

关键词：维生素U S-甲基甲硫氨酸液相色谱检测方法

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卷心菜清汁饮料的酶解工艺研究

食品科技 2020年第4期45卷 96-101页

作者：朱碧云何艳秦博文马霞上海应用技术大学香料香精技术与工程学院上海201418 上海禾文农业科技有限公司上海201107

以卷心菜为原料,利用纤维素酶、果胶酶和木聚糖酶复合制取卷心菜清汁饮料,并通过Box-Behnken响应面法优化酶解工艺条件。结果表明,卷心菜清汁饮料酶解的最佳工艺参数为:酶解时间86 min、酶解温度51 ℃、复合酶配比(纤维素酶:果胶酶:木... 详细信息

以卷心菜为原料,利用纤维素酶、果胶酶和木聚糖酶复合制取卷心菜清汁饮料,并通过Box-Behnken响应面法优化酶解工艺条件。结果表明,卷心菜清汁饮料酶解的最佳工艺参数为:酶解时间86 min、酶解温度51 ℃、复合酶配比(纤维素酶:果胶酶:木聚糖酶)0.1%:0.2%:0.08%,在此工艺条件下,卷心菜清汁饮料的酶解效果最佳。

关键词：卷心菜清汁饮料响应面法酶解

权力感与在线评论类型的匹配对消费者购买意愿的影响

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上海管理科学 2019年第3期41卷 43-47页

作者：朱碧筠周洁如上海交通大学安泰经济与管理学院

主要研究了在线评论类型和消费者的权力感对其购买意愿的影响。实验研究发现,消费者不同的权力感会影响其在看到不同类型的在线评论后所产生的购买意愿,其机制在于高(低)权力感的人在浏览体现工具性(认同性)利益的在线评论后会产生更高... 详细信息

主要研究了在线评论类型和消费者的权力感对其购买意愿的影响。实验研究发现,消费者不同的权力感会影响其在看到不同类型的在线评论后所产生的购买意愿,其机制在于高(低)权力感的人在浏览体现工具性(认同性)利益的在线评论后会产生更高的信息加工流畅性,从而增加其购买意愿。此外,探讨了研究成果的理论意义和对实践的启示,并提出了研究的局限性与未来的研究方向。

关键词：权力感在线评论类型加工流畅性购买意愿

EB病毒阳性淋巴上皮瘤样胆管细胞癌3例临床病理学分析

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中华病理学杂志 2023年第8期52卷 841-843页

作者：杜明占郭凌川黄山郭霞朱碧云苏州大学附属第一医院病理科苏州215006 苏州大学附属第一医院放射科苏州215006

目的探讨EB病毒阳性淋巴上皮瘤样胆管细胞癌的临床病理特征。方法收集苏州大学附属第一医院2008—2022年诊断的EB病毒阳性淋巴上皮瘤样胆管细胞癌3例,分析临床特征、HE和免疫组织化学特点并总结。结果3例均为女性;平均年龄53.7岁(51~59... 详细信息

目的探讨EB病毒阳性淋巴上皮瘤样胆管细胞癌的临床病理特征。方法收集苏州大学附属第一医院2008—2022年诊断的EB病毒阳性淋巴上皮瘤样胆管细胞癌3例,分析临床特征、HE和免疫组织化学特点并总结。结果3例均为女性;平均年龄53.7岁(51~59岁);1例位于肝左叶,2例于肝右叶。1例血清乙肝病毒阳性,2例阴性。2例术前血清甲胎蛋白水平正常,1例未测。镜下瘤细胞部分为实性、未分化形态,部分呈腺管状,间质淋巴细胞、浆细胞丰富,部分淋巴滤泡形成。2例伴有普通型胆管细胞癌,显示不规则腺体伴有促纤维性间质及散在淋巴细胞、浆细胞。周围肝组织均未见肝硬化,1例伴有重度脂肪变性。EB病毒阳性淋巴上皮瘤样胆管细胞癌和普通型胆管细胞癌区域,免疫组织化学均细胞角蛋白(CK)7、CK19阳性,错配修复蛋白均提示微卫星稳定,原位杂交检测均提示EB病毒编码的RNA阳性。随访时间4~20个月,均生存。结论EB病毒阳性淋巴上皮瘤样胆管细胞癌是一种少见的肝内肿瘤类型,需EB病毒检测,其具有独特的组织学形态和免疫组织化学特点。

关键词：胆管细胞癌血清甲胎蛋白组织学形态肝左叶肝右叶脂肪变性瘤细胞 EB病毒