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Aspartoacylase suppresses prostate cancer progression by blocking LYN activation

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Military Medical Research 2024年第2期11卷 180-205页

作者： Hong Weng Kang-Ping Xiong Wang Wang Kai-Yu Qian Shuai Yuan Gang Wang Fang Yu jun Luo Meng‑Xin Lu zhong‑Hua Yang Tao Liu Xing Huang Hang Zheng Xing-Huan Wang Department of Urology Zhongnan Hospital of Wuhan UniversityWuhan 430071China Center for Evidence‑Based and Translational Medicine Zhongnan Hospital of Wuhan UniversityWuhan 430071China Department of Biological Repositories Zhongnan Hospital of Wuhan UniversityWuhan 430071China Hubei Key Laboratory of Tumor Biological Behaviors Wuhan 430071China Department of Pathology Zhongnan Hospital of Wuhan UniversityWuhan 430071China Center for Pathology and Molecular Diagnostics Wuhan UniversityWuhan 430071China Wuhan Research Center for Infectious Diseases and Cancer Chinese Academy of Medical SciencesWuhan 430071China

Background:Globally,despite prostate cancer(PCa)representing second most prevalent malignancy in male,the precise molecular mechanisms implicated in its pathogenesis remain ***,elucidating the key molecular regulators that govern disease progression could substantially contribute to the establishment of novel therapeutic strategies,ultimately advancing the management of ***:A total of 49 PCa tissues and 43 adjacent normal tissues were collected from January 2017 to December 2021 at zhongnan Hospital of Wuhan *** advanced transcriptomic methodologies were employed to identify differentially expressed mRNAs in *** expression of aspartoacylase(ASPA)in PCa was thoroughly evaluated using quantitative real-time PCR and Western blotting *** elucidate the inhibitory role of ASPA in PCa cell proliferation and metastasis,a comprehensive set of in vitro and in vivo assays were conducted,including orthotopic and tumor-bearing mouse models(n=8 for each group).A combination of experimental approaches,such as Western blotting,luciferase assays,immunoprecipitation assays,mass spectrometry,glutathione S-transferase pulldown experiments,and rescue studies,were employed to investigate the underlying molecular mechanisms of ASPA's action in *** Student‘s t-test was employed to assess the statistical significance between two distinct groups,while one-way analysis of variance was utilized for comparisons involving more than two groups.A two-sided P<0.05 was deemed to indicate statistical ***:ASPA was identified as a novel inhibitor of PCa *** expression of ASPA was found to be significantly down-regulated in PCa tissue samples,and its decreased expression was independently associated with patients’prognosis(HR=0.60,95%CI 0.40–0.92,P=0.018).Our experiments demonstrated that modulation of ASPA activity,either through gain-or loss-of-function,led to the suppression or enhancement of PCa cell proliferation,mig

关键词： Prostate cancer Aspartoacylase LYN JNK AP-1 C-jun Phosphorylation

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Novel miR-490-3p/hnRNPA1-b/PKM2 axis mediates the Warburg effect and proliferation of colon cancer cells via the PI3K/AKT pathway

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World Journal of Gastrointestinal Oncology 2024年第5期16卷 2038-2059页

作者： Xiang-Hui Wan Guo-Bing Jin Qun Yang Ji-Long Hu Zhi-Liang Liu jun Rao Can Wen Peng-Ling Li Xi-Mei Yang Bo Huang Xiao-zhong Wang Jiangxi Medical College Nanchang UniversityNanchang 330029Jiangxi ProvinceChina Department of Clinical Laboratory Jiangxi Cancer HospitalNanchang 330029Jiangxi ProvinceChina Jiangxi Key Laboratory of Translational Research for Cancer Jiangxi Cancer HospitalNanchang 330029Jiangxi ProvinceChina Department of Abdominal Surgery Jiangxi Cancer HospitalNanchang 330029Jiangxi ProvinceChina Department of Pathology Jiangxi Cancer HospitalNanchang 330029Jiangxi ProvinceChina Science and Education Section Jiangxi Cancer HospitalNanchang 330029Jiangxi ProvinceChina Department of Clinical Laboratory Jiangxi Children’s HospitalNanchang 330006Jiangxi ProvinceChina Department of Clinical Laboratory The Second Affiliated Hospital of Nanchang UniversityNanchang 330006Jiangxi ProvinceChina Jiangxi Province Key Laboratory of Laboratory Medicine The Second Affiliated Hospital of Nanchang UniversityNanchang 330006Jiangxi ProvinceChina

BACKGROUND Heterogeneous ribonucleoprotein A1(hnRNPA1)has been reported to enhance the Warburg effect and promote colon cancer(CC)cell proliferation,but the role and mechanism of the miR-490-3p/hnRNPA1-b/PKM2 axis in CC have not yet been *** To investigate the role and mechanism of a novel miR-490-3p/hnRNPA1-b/PKM2 axis in enhancing the Warburg effect and promoting CC cell proliferation through the PI3K/AKT *** Paraffin-embedded pathological sections from 220 CC patients were collected and subjected to immunohistochemical analysis to determine the expression of *** relationship between the expression values and the clinicopathological features of the patients was *** in mRNA expression were analyzed using quantitative real-time polymerase chain reaction,while differences in protein expression were analyzed using western *** proliferation was evaluated using the cell counting kit-8 and 5-ethynyl-2’-deoxyuridine assays,and cell cycle and apoptosis were detected using flow cytometric *** targeted binding of miR-490-3p to hnRNPA1-b was validated using a dual luciferase reporter *** Warburg effect was evaluated by glucose uptake and lactic acid production *** The expression of hnRNPA1-b was significantly increased in CC tissues and cells compared to normal controls(P<0.05).Immunohistochemical results demonstrated significant variations in the expression of the hnRNPA1-b antigen in different stages of CC,including stage I,II-III,and ***,the clinicopathologic characterization revealed a significant correlation between hnRNPA1-b expression and clinical stage as well as T ***1-b was found to enhance the Warburg effect through the PI3K/AKT pathway,thereby promoting proliferation of HCT116 and SW620 ***,the proliferation of HCT116 and SW620 cells was inhibited when miR-490-3p targeted and bound to hnRNPA1-b,effectively blocking the Warburg ***

关键词： Heterogeneous ribonucleoprotein A1-b MiR-490-3p Colon cancer Alternative splicing Warburg effect

High-yield and rapid isolation of extracellular vesicles by flocculation via orbital acoustic trapping:FLOAT

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Microsystems & Nanoengineering 2024年第1期10卷 313-323页

作者： Joseph Rufo Peiran Zhang Zeyu Wang Yuyang Gu Kaichun Yang Joseph Rich Chuyi Chen Ruoyu zhong Ke Jin Ye He Jianping Xia Ke Li Jiarong Wu Yingshi Ouyang Yoel Sadovsky Luke P.Lee Tony jun Huang Department of Mechanical Engineering and Materials Science Duke UniversityDurhamNCUSA Department of Biomedical Engineering Duke UniversityDurhamNCUSA Department of Obstetrics Gynecologyand Reproductive SciencesMagee-Womens Research InstituteUniversity of PittsburghPittsburghPAUSA Department of Microbiology and Molecular Genetics School of MedicineUniversity of PittsburghPittsburghPAUSA Renal Division and Division of Engineering in Medicine Department of MedicineBrigham and Women’s HospitalHarvard Medical SchoolBostonMAUSA Department of Bioengineering Department of Electrical Engineering and Computer ScienceUniversity of CaliforniaBerkeleyBerkeleyCAUSA Department of Biophysics Institute of Quantum BiophysicsSungkyunkwan UniversitySuwonKorea

Extracellular vesicles(EVs)have been identified as promising biomarkers for the noninvasive diagnosis of various ***,challenges in separating EVs from soluble proteins have resulted in variable EV recovery rates and low ***,we report a high-yield(>90%)and rapid(<10 min)EV isolation method called FLocculation via Orbital Acoustic Trapping(FLOAT).The FLOAT approach utilizes an acoustofluidic droplet centrifuge to rotate and controllably heat liquid *** adding a thermoresponsive polymer flocculant,nanoparticles as small as 20 nm can be rapidly and selectively concentrated at the center of the *** demonstrate the ability of FLOAT to separate urinary EVs from the highly abundant Tamm-Horsfall protein,addressing a significant obstacle in the development of EV-based liquid *** to its high-yield nature,FLOAT reduces biofluid starting volume requirements by a factor of 100(from 20 mL to 200µL),demonstrating its promising potential in point-of-care diagnostics.

关键词： trapping utilize separating

Aerosol jet printing of surface acoustic wave microfluidic devices

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Microsystems & Nanoengineering 2024年第1期10卷 231-242页

作者： Joseph Rich Brian Cole Teng Li Brandon Lu Hanyu Fu Brittany N.Smith Jianping Xia Shujie Yang Ruoyu zhong James L.Doherty Kanji Kaneko Hiroaki Suzuki Zhenhua Tian Aaron D.Franklin Tony jun Huang Department of Biomedical Engineering Duke UniversityDurhamNC 27708USA Department of Electrical and Computer Engineering Duke UniversityDurhamNC 27708USA Department of Mechanical Engineering Virginia Polytechnic Institute and State UniversityBlacksburgVA 24061USA Thomas Lord Department of Mechanical Engineering and Materials Science Duke UniversityDurhamNC 27708USA Deptartment of Precision Mechanics Faculty of Science and EngineeringChuo UniversityTokyo 112-8551Japan Department of Chemistry Duke UniversityDurhamNC 27708USA

The addition of surface acoustic wave(SAW)technologies to microfluidics has greatly advanced lab-on-a-chip applications due to their unique and powerful attributes,including high-precision manipulation,versatility,integrability,biocompatibility,contactless nature,and rapid ***,the development of SAW microfluidic devices is limited by complex and time-consuming micro/nanofabrication techniques and access to cleanroom facilities for multistep photolithography and vacuum-based *** simplify the fabrication of SAW microfluidic devices with customizable dimensions and functions,we utilized the additive manufacturing technique of aerosol jet *** successfully fabricated customized SAW microfluidic devices of varying materials,including silver nanowires,graphene,and poly(3,4-ethylenedioxythiophene)polystyrene sulfonate(PEDOT:PSS).To characterize and compare the acoustic actuation performance of these aerosol jet printed SAW microfluidic devices with their cleanroom-fabricated counterparts,the wave displacements and resonant frequencies of the different fabricated devices were directly measured through scanning laser Doppler ***,to exhibit the capability of the aerosol jet printed devices for lab-on-a-chip applications,we successfully conducted acoustic streaming and particle concentration ***,we demonstrated a novel solution-based,direct-write,single-step,cleanroom-free additive manufacturing technique to rapidly develop SAW microfluidic devices that shows viability for applications in the fields of biology,chemistry,engineering,and medicine.

关键词： printing wave fluid

Serum inflammatory markers in children with Mycoplasma pneumoniae pneumonia and their predictive value for mycoplasma severity

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World Journal of Clinical Cases 2024年第22期12卷 4940-4946页

作者： Li-Ping Wang zhong-Hua Hu jun-Sheng Jiang Jie Jin Department of Pediatrics The First People's Hospital of Linping DistrictHangzhou 310000Zhejiang ProvinceChina Department of Pediatrics Changzhou Children HospitalChangzhou 213100Jiangsu ProvinceChina

BACKGROUND Mycoplasma pneumoniae pneumonia(MPP)significantly impacts pediatric health,necessitating markers for early severe disease *** To investigate the correlation between serum inflammatory marker and the severity of MPP in *** A prospective study was carried out from January 2023 to November 2023.A total of 160 children with MPP who underwent treatment were selected:80 had severe MPP and 80 had mild *** and laboratory data were collected at the time of hospital admission and during *** operating characteristic curves were utilized to assess the diagnostic and prognostic for severe *** Fever duration and length of hospitalization in pediatric patients with severe MPP exceeded those with mild *** incidence of pleural effusion,lung consolidation,and bronchopneumonia on imaging was markedly elevated in the severe MPP cohort compared to the mild MPP *** contrast to the mild cohort,there was a notable increase in C-reactive protein(CRP),procalcitonin(PCT),erythrocyte sedimentation rate,lactic dehydrogenase,D-dimer,and inflammatory cytokines[interleukin(IL)-6,IL-8,IL-10 and tumor necrosis factor(TNF)-α]in the severe MPP group were significantly *** Serum inflammatory markers(CRP,PCT,IL-6,D-dimer,IL-10 and TNF-α)were considered as predictors in children with severe MPP.

关键词： Cytokine Mycoplasma pneumoniae pneumonia Children,Community-acquired pneumonia levels Interleukin-6 D-dimer

Mechanisms of vacuolar phosphate efflux supporting soybean root hair growth in response to phosphate deficiency

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Journal of Integrative Plant Biology 2024年第09期 1983-1999页

作者： zhong Shan Yanli Chu Guangfang Sun Rui Chen jun Yan Qiwei He Yingna Liu Bin Wang Mingda Luan Wenzhi Lan School of Life Sciences Nanjing University Institute of Future Agriculture Northwest A&F University

Phosphorus is an essential macronutrient for plant growth and development. In response to phosphate(Pi) deficiency, plants rapidly produce a substitutive amount of root hairs; however, the mechanisms underlying Pi supply for root hair growth remain unclear. Here, we observed that soybean(Glycine max) plants maintain a consistent level of Pi within root hairs even under external Pi deficiency. We therefore investigated the role of vacuole-stored Pi, a major Pi reservoir in plant cells, in supporting root hair growth under Pi-deficient conditions. Our findings indicated that two vacuolar Pi efflux(VPE) transporters, Gm VPE1 and Gm VPE2, remobilize vacuolar stored Pi to sustain cytosolic Pi content in root hair *** analysis showed that double mutants of Gm VPE1 and Gm VPE2 exhibited reduced root hair growth under low Pi conditions. Moreover,Gm VPE1 and Gm VPE2 were highly expressed in root hairs, with their expression levels significantly upregulated by low Pi treatment. Further analysis revealed that Gm RSL2(ROOT HAIR DEFECTIVE6-like 2), a transcription factor involved in root hair morphogenesis, directly binds to the promoter regions of Gm VPE1 and Gm VPE2, and promotes their expressions under low Pi conditions. Additionally, mutants lacking both Gm RSL2 and its homolog Gm RSL3 exhibited impaired root hair growth under low Pi stress, which was rescued by overexpressing either Gm VPE1 or Gm *** together, our study has identified a module comprising vacuolar Pi exporters and transcription factors responsible for remobilizing vacuolar Pi to support root hair growth in response to Pi deficiency in soybean.

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Initial COVID-19 severity influenced by SARS-CoV-2-specific T cells imprints T-cell memory and inversely affects reinfection

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Signal Transduction and Targeted Therapy 2024年第6期9卷 2791-2803页

作者： Gang Yang Jinpeng Cao Jian Qin Xinyue Mei Shidong Deng Yingjiao Xia jun Zhao junxiang Wang Tao Luan Daxiang Chen Peiyu Huang Cheng Chen Xi Sun Qi Luo Jie Su Yunhui Zhang Nanshan zhong zhongfang Wang The Affiliated Hospital of Kunming University of Science and Technology.Department of Respiratory and Critical Care Medicine The First People’s Hospital of Yunnan provinceKunmingYunnanChina Guangzhou National Laboratory BiolandGuangzhouGuangdongChina State Key Laboratory of Respiratory Disease&National Clinical Research Center for Respiratory Disease Guangzhou Institute of Respiratory Healththe First Affiliated Hospital of Guangzhou Medical UniversityGuangzhou Medical UniversityGuangzhouChina

The immunoprotective components control COVID-19 disease severity,as well as long-term adaptive immunity maintenance and subsequent reinfection risk discrepancies across initial COVID-19 severity,remain ***,we longitudinally analyzed SARS-CoV-2-specific immune effectors during the acute infection and convalescent phases of 165 patients with COVID-19 categorized by *** found that early and robust SARS-CoV-2-specific CD4^(+)and CD8^(+)T cell responses ameliorate disease progression and shortened hospital stay,while delayed and attenuated virus-specific CD8^(+)T cell responses are prominent severe COVID-19 *** antiviral antibody generation rather than titer level associates with severe ***,initial COVID-19 severity imprints the long-term maintenance of SARS-CoV-2-specific adaptive immunity,demonstrating that severe convalescents exhibited more sustained virus-specific antibodies and memory T cell responses compared to mild/moderate ***,initial COVID-19 severity inversely correlates with SARS-CoV-2 reinfection ***,our study unravels the complicated interaction between temporal characteristics of virus-specific T cell responses and COVID-19 severity to guide future SARS-CoV-2 wave management.

关键词： severity immunity infection

Pulse repetition rate effect on the plasma inside femtosecond laser filament in air

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Chinese Optics Letters 2024年第1期22卷 135-140页

作者：尹富康王铁军刘尧香龙娟魏迎霞朱斌周凯南冷雨欣 State Key Laboratory of High Field Laser Physics Shanghai Institute of Optics and Fine Mechanics and CAS Center for Excellence in Ultra-intense Laser ScienceChinese Academy of SciencesShanghai 201800China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China Laser Fusion Research Center and Science&Technology on Plasma Physics Laboratory China Academy of Engineering PhysicsMianyang 621999China

The characteristics of plasmas play an important role in femtosecond laser filament-based *** analysis is used to experimentally investigate the plasma density and its temperature of the air filament under different pulse repetition *** our experiments,the measured average plasma density of the filament is 1.54×10^(17)cm^(-3)and the temperature of the plasma is about 5100 K under 100 Hz pulse repetition *** plasma density decreases to1.43×10^(17)cm^(-3)and the temperature increases to 6230 K as the pulse repetition rate increases to 1000 *** experimental observation agrees with the numerical simulation by solving the nonlinear Schr?dinger equations with repetition rate related“low density hole”correction.

关键词： femtosecond laser filamentation cumulative effects electron density

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新媒介何以“持续生长”:数字媒介生态视角下的数字可持续性

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编辑之友 2024年第1期32卷 92-99页

作者：金圣钧钟新中国人民大学新闻学院北京100872 中国人民大学新闻与社会发展研究中心北京100872

近年来,受到本土功能主义应用式传播研究风气的影响,相关研究对数字媒介生态理论维度和分析视角的革新、凝练和批判有所欠缺。文章在梳理对媒介生态学相关误读的基础上,探讨了从传统媒介生态学到数字媒介生态的进化机理与内涵变迁。在... 详细信息

近年来,受到本土功能主义应用式传播研究风气的影响,相关研究对数字媒介生态理论维度和分析视角的革新、凝练和批判有所欠缺。文章在梳理对媒介生态学相关误读的基础上,探讨了从传统媒介生态学到数字媒介生态的进化机理与内涵变迁。在此基础上,引入数字可持续性概念,从数字平台生态系统、数字媒介时间的生态学层面和数字保存、数字共享的传播层面解读数字可持续性的内涵和发展方向,关注在媒介生态变迁中如何延长数字寿命等问题。文章认为,数字媒介运作中物质与非物质、人类与非人类等因素如何在特定语境中通过组合实现持续性生长,是研究数字媒介生态时需长久关注的问题。

关键词：数字媒介生态数字可持续性媒介生态学数字化传播媒介技术

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