Novel miR-490-3p/hnRNPA1-b/PKM2 axis mediates the Warburg effect and proliferation of colon cancer cells via the PI3K/AKT pathway

作     者：Xiang-Hui Wan Guo-Bing Jin Qun Yang Ji-Long Hu Zhi-Liang Liu Jun Rao Can Wen Peng-Ling Li Xi-Mei Yang Bo Huang Xiao-Zhong Wang 

作者机构：Jiangxi Medical CollegeNanchang UniversityNanchang 330029Jiangxi ProvinceChina Department of Clinical LaboratoryJiangxi Cancer HospitalNanchang 330029Jiangxi ProvinceChina Jiangxi Key Laboratory of Translational Research for CancerJiangxi Cancer HospitalNanchang 330029Jiangxi ProvinceChina Department of Abdominal SurgeryJiangxi Cancer HospitalNanchang 330029Jiangxi ProvinceChina Department of PathologyJiangxi Cancer HospitalNanchang 330029Jiangxi ProvinceChina Science and Education SectionJiangxi Cancer HospitalNanchang 330029Jiangxi ProvinceChina Department of Clinical LaboratoryJiangxi Children’s HospitalNanchang 330006Jiangxi ProvinceChina Department of Clinical LaboratoryThe Second Affiliated Hospital of Nanchang UniversityNanchang 330006Jiangxi ProvinceChina Jiangxi Province Key Laboratory of Laboratory MedicineThe Second Affiliated Hospital of Nanchang UniversityNanchang 330006Jiangxi ProvinceChina 

出 版 物：《World Journal of Gastrointestinal Oncology》 (世界胃肠肿瘤学杂志（英文版）（电子版）)

年 卷 期：2024年第16卷第5期

页      面：2038-2059页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Supported by the National Natural Science Foundation of China,No.82160405 Jiangxi Provincial Natural Science Foundation,No.20232BAB206131,No.20212ACB206016,and No.20224BAB206114 Jiangxi Provincial Health Commission Project,No.202310887 the Development Fund of Jiangxi Cancer Hospital,No.2021J10 

主　　题：Heterogeneous ribonucleoprotein A1-b MiR-490-3p Colon cancer Alternative splicing Warburg effect 

摘      要：BACKGROUND Heterogeneous ribonucleoprotein A1(hnRNPA1)has been reported to enhance the Warburg effect and promote colon cancer(CC)cell proliferation,but the role and mechanism of the miR-490-3p/hnRNPA1-b/PKM2 axis in CC have not yet been *** To investigate the role and mechanism of a novel miR-490-3p/hnRNPA1-b/PKM2 axis in enhancing the Warburg effect and promoting CC cell proliferation through the PI3K/AKT *** Paraffin-embedded pathological sections from 220 CC patients were collected and subjected to immunohistochemical analysis to determine the expression of *** relationship between the expression values and the clinicopathological features of the patients was *** in mRNA expression were analyzed using quantitative real-time polymerase chain reaction,while differences in protein expression were analyzed using western *** proliferation was evaluated using the cell counting kit-8 and 5-ethynyl-2’-deoxyuridine assays,and cell cycle and apoptosis were detected using flow cytometric *** targeted binding of miR-490-3p to hnRNPA1-b was validated using a dual luciferase reporter *** Warburg effect was evaluated by glucose uptake and lactic acid production *** The expression of hnRNPA1-b was significantly increased in CC tissues and cells compared to normal controls(P0.05).Immunohistochemical results demonstrated significant variations in the expression of the hnRNPA1-b antigen in different stages of CC,including stage I,II-III,and ***,the clinicopathologic characterization revealed a significant correlation between hnRNPA1-b expression and clinical stage as well as T ***1-b was found to enhance the Warburg effect through the PI3K/AKT pathway,thereby promoting proliferation of HCT116 and SW620 ***,the proliferation of HCT116 and SW620 cells was inhibited when miR-490-3p targeted and bound to hnRNPA1-b,effectively blocking the Warburg ***