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Analysis of differential expression of tight junction proteins in cultured oral epithelial cells altered by Porphyromonas gingivalis,Porphyromonas gingivalis lipopolysaccharide,and extracellular adenosine triphosphate

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International Journal of Oral Science 2017年第4期9卷 238-244页

作者： wei guo peng wang zhong-hao liu ping ye department of endodontics yantai stomatological hospitalbinzhou medical universityYantaiChina department of pediatrics yantai stomatological hospitalbinzhou medical universityYantaiChina department of implant yantai stomatological hospitalbinzhou medical universityYantaiChina institute of dental research centre for oral healthwestmead hospitalWestmeadAustralia faculty of dentistry the university of sydneySydneyAustralia

Tight junctions （TJs） are the most apical intercellular junctions of epithelial cells formed by occludin, claudins, junctional adhesion molecules （JAMs）, and zonula occludens （ZO）. Tight junction proteins can sense the presence of bacteria and regulate the transcription of target genes that encode effectors and regulators of the immune response. The aim of this study was to determine the impact of TJ proteins in response to Porphyromonas gingivalis （P. gingivalis）, P. gingivalis lipopolysaccharide （P. gingivalis LPS）, and extracellular adenosine triphosphate （ATP） in the oral epithelial cell culture model. Quantified real time- polymerase chain reaction （RT-PCR）, immunoblots, and immunostaining were performed to assess the gene and protein expression in TJs. It was found that P. gingivalis infection led to transient upregulation of the genes encoding occludin, claudin- 1, and claudin-4 but not JAM-A, claudin-15, or ZO-1, while P. gingivalis LPS increased claudin-1, claudin-15, and ZO-1 and decreased occludin, JAM-A, and claudin-4. Tight junction proteins showed significant upregulation in the above two groups when cells were pretreated with ATP for 3 h. The findings indicated that P. gingivalis induced the host defence responses at an early stage. P. gingivalis LPS exerted a more powerful stimulatory effect on the disruption of the epithelial barrier than P. gingivalis. ATP stimulation enhanced the reaction of TJ proteins to P. gingivalis invasion and LPS destruction of the epithelium.

关键词： junctional epithelium periodontitis Porphyromonas gingivalis tight junctions

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High-resolution ARPES endstation for in situ electronic structure investigations at SSRF

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Nuclear Science and Techniques 2021年第3期32卷 94-106页

作者： Yi-Chen Yang Zheng-Tai liu Ji-Shan liu zhong-hao liu Wan-Ling liu Xiang-Le Lu Hong-Ping Mei Ang Li Mao Ye Shan Qiao Da-Wei Shen State Key Laboratory of Functional Materials for Informatics Shanghai Institute of Microsystem and Information TechnologyChinese Academy of SciencesShanghai 200050China University of Chinese Academy of Sciences Beijing 100049China

Angle-resolved photoemission spectroscopy(ARPES)is one of the most powerful experimental techniques in condensed matter *** ARPES,which uses photons with high flux and continuously tunable energy,has become particularly ***,an excellent synchrotron ARPES system must have features such as a small beam spot,super-high energy resolution,and a user-friendly operation interface.A synchrotron beamline and an endstation(BL03 U)were designed and constructed at the Shanghai Synchrotron Radiation *** beam spot size at the sample position is 7.5(V)μm×67(H)μm,and the fundamental photon range is 7-165 eV;the ARPES system enables photoemission with an energy resolution of 2.67 meV at21.2 *** addition,the ARPES system of this endstation is equipped with a six-axis cryogenic sample manipulator(the lowest temperature is 7 K)and is integrated with an oxide molecular beam epitaxy system and a scanning tunneling microscope,which can provide an advanced platform for in situ characterization of the fine electronic structure of condensed matter.

关键词： Synchrotron ARPES In situ VUV laser Quantum materials

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Investigation of the room-temperature photoelectron spectroscopy of type-Ⅱ Weyl semimetal candidate WTe_(2)

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Tungsten 2023年第3期5卷 350-356页

作者： Jian-Yang Ding Zheng-Tai liu Zhe Huang Zhi-Cheng Jiang Yi-Chen Yang zhong-hao liu Ji-Shan liu Yan-Feng Guo Da-Wei Shen State Key Laboratory of Functional Materials for Informatics Shanghai Institute of Microsystem and Information TechnologyChinese Academy of SciencesShanghai 200050China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China School of Physical Science and Technology ShanghaiTech UniversityShanghai 201210China

Layered transition metal dichalcogenides have novel physical properties and great potential for *** them,WTe2,which has an extremely large unsaturated magnetoresistance and is theoretically predicted to be a type-Ⅱ Weyl semimetal,has been extensively ***,we systematically probe the electronic structure of WTe_(2) at room temperature using high-resolution angle-resolved photoelectron spectroscopy(ARPES).We find that temperature-driven chemical potential shift and Lifshitz transition,which is equivalent to low-energy band structures shift downward by around 50 meV,compared to the results at low *** ARPES experimental results match well with previous theoretical calculations,implying the possible existence of type-Ⅱ Weyl points near the Γ-X ***,as expected,there exists a dominantly electron-like Fermi surface instead of the one with compensated electrons and ***,our ARPES results show that the flat band(FB) lying below the Fermi level(EF) becomes closer to the Fermi level at room temperature,which might start to dominate the transport behavior and lead to the disappearance of the unsaturated giant magnetoresistance *** findings not only reveal the electronic structure features of WTe_(2) at room temperature,but also provide new insights into the development of room-temperature topological quantum devices.

关键词： Weyl semimetal Electronic structure ARPES Compensation effect

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Two Palatal Root Canals in a Maxillary Right Second Molar:One Case Report

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口腔医学研究 2009年第3期25卷 356-357页

作者： Zhi - shah Lu zhong - hao liu Hong Li Guang - shui Jiang Yantai Stomatological Hospital Yantai 264000 School of Stomatology Shandong University

牙根和根管的数目不是固定不变的,文献报道表明牙齿尤其是上颌磨牙根管存在着解剖变异。但是,上颌第二磨牙发生腭根双根管的可能性很低。在本文中,作者报道了一例右上颌第二磨牙腭根双根管的解剖变异。由于可能存在的上颌第二磨牙腭根... 详细信息

牙根和根管的数目不是固定不变的,文献报道表明牙齿尤其是上颌磨牙根管存在着解剖变异。但是,上颌第二磨牙发生腭根双根管的可能性很低。在本文中,作者报道了一例右上颌第二磨牙腭根双根管的解剖变异。由于可能存在的上颌第二磨牙腭根管解剖变异,所以临床医生在牙髓病治疗过程中应注意术前对患牙进行影像学分析,术中可借助牙科手术显微镜等可视手段寻找可能存在的腭根解剖变异。

关键词：上颌第二磨牙腭根双根管牙科显微镜

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Growth of high-quality perovskite (110)-SrIrO_3 thin films using reactive molecular beam epitaxy

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Chinese Physics B 2018年第8期27卷 578-581页

作者： Kai-Li Zhang Cong-Cong Fan Wan-Ling liu Yv -Feng Wu Xiang-Le Lu Zheng-Tai liu Ji-Shan liu zhong-hao liu Da-Wei Shen State Key Laboratory of Functional Materials for Informatics Shanghai Institute of Microsystem and Information Technoloy（SIMIT）Chinese Academy of Sciences（CAS）Shanghai 200050Chia University of Chinese Academy of Sciences Beijing 100049China School of Physicai Science and Technology ShanghaiTech UniversityShanghai 200031China CAS Center for Excellence in Superconducting Electronics（CENSE） Shanghai 200050China

Recently, 5d transition metal iridates have been reported as promising materials for the manttfacture of exotic quan- tum states. Apart from the semimetallic ground states that have been observed, perovskite SrlrO3 is also predicted to have a lattice-symmetrically protected topological state in the （110） plane due to its strong： spin-orbil coupling and electron correlation. Compared with non-polar （001）-SflrO3, the especial polarity of （110）-SrIrC）3 undoubtedly adds the： difficulty of fabrication and largely impedes the research on its surface states. Here, we have successfully synthesized high-quality （110）-SflrO3 thin films on （110）-SrTiO3 substrates by reactive molecular beam epitaxy fi^r the first time. Both reflec- tion high-energy electron diffraction pattems and x-ray diffraction measurements suggest the expected orientation and outstanding crystallinity. A （1 × 2） surface reconstruction driven from the surface instabiJity, the. same as that reported in （110）-SrTiO3, is observed. The electric transport measurements uncover that （110）-SrIrO3 exhibits a more prominent semimetallic property in comparison to （001）-SrIrO3.

关键词： molecular beam epitaxy iridates topological crystalline metal sunace reconstruction

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Electronic structure of single-crystalline graphene grown on Cu/Ni(111) alloy film

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Chinese Physics B 2019年第8期28卷 250-253页

作者： Xue-Fu Zhang zhong-hao liu Wan-Ling liu Xiang-Le Lu Zhuo-Jun Li Qing-Kai Yu Da-Wei Shen Xiao-Ming Xie State Key Laboratory of Functional Materials for Informatics Shanghai Institute of Microsystem and Information Technology (SIMIT)Chinese Academy of SciencesShanghai 200050China University of Chinese Academy of Sciences Beijing 100049China School of Physical Science and Technology Shanghai Tech UniversityShanghai 200031China CAS Center for Excellence in Superconducting Electronics (CENSE) Shanghai 200050China

Graphene with a Dirac cone-like electronic structure has been extensively studied because of its novel transport properties and potential application for future electronic *** epitaxially grown graphene,the process conditions and the microstructures are strongly dependent on various substrate materials with different lattice constants and interface *** angle-resolved photoemission spectroscopy,here we report an investigation of the electronic structure of single-crystalline graphene grown on Cu/Ni(111)alloy film by chemical vapor *** a relatively low growth temperature,graphene on Cu/Ni(111)exhibits a Dirac cone-like dispersion comparable to that of graphene grown on Cu(111).The linear dispersions forming Dirac cone are as wide as 2 e V,with the Fermi velocity of approximately 1.1×10^6 m/*** cone opens a gap of approximately 152 meV at the binding energy of approximately 304 *** findings would promote the study of engineering of graphene on different substrate materials.

关键词： single-crystal graphene electronic structure Cu/Ni(111)

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Influence of heat treatment on microstructure of a newβ-solidifyingγ-TiAl alloy

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Journal of Iron and Steel Research International 2025年第1期32卷 239-248页

作者： Li Quan Xiao-bing Li Peng Xue Jun-jie hao Kun Qian Bo Chen Jian-zhong Li Kui liu School of Metallurgy Northeastern UniversityShenyang110819LiaoningChina Department of Materials Science and Technology Ji Hua LaboratoryFoshan528200GuangdongChina

The high strength and stability of the full-lamellar structure guarantee the industrial application of theβ-solidifyingγ-TiAl ***,it is a huge challenge to design an alloy with good hot-deformability as well as the full-lamellar *** low-cost Ti-42.5Al-2Mn-0.4Mo-0.1B-0.1C(at.%)alloy was designed,which undergoes bothβandαsingle-phase region during the *** is found that the full-lamellar structure can be obtained by the solution heat treatment at 1230℃ for 20 min and then aging treatment at 800℃ for 3 ***,a new microstructure,namely,the pearlitic-like microstructure(PM)induced by theα_(2)/γ→βo+γcellular reaction was observed when the aging temperature is increased to above 800℃.The volume fraction of the PM is gradually increased from 0%to 25.5%,65%,and 94%according to elevated aging temperature from 800 to 900,1000,and 1050℃,*** mechanism of the reducedα_(2)/γlamellae and PM formation was discussed regarding the heterogeneous distribution ofβstabilizing elements and the interface energy stored inα_(2)/γlamellae.

关键词： β-γ-TiAl Alloy design Two-step heat treatment Full-lamellar structure Cellular reaction

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Horizontal transposon transfer during plant terrestrialization

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Journal of Integrative Plant Biology 2025年第1期67卷 15-18页

作者： hao Wang Zilong Xu Zhenhua Zhang Bojian zhong College of Life Sciences Nanjing Normal UniversityNanjing 210023China

Horizontal gene transfer(HGT)is the sharing of genetic material between reproductively isolated *** process breaks the boundaries of genetic relatedness between organisms and enables the recipient to rapidly acquire n... 详细信息

关键词： species transfer reproductive

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g-C_(3)N_(4)nanosheets coupled with CoSe_(2)as co-catalyst for efficient photooxidation of xylose to xylonic acid

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Green Energy & Environment 2025年第1期10卷 231-238页

作者： Qi hao Yijun liu Ren Zou Ge Shi Shilian Yang Linxin zhong Wu Yang Xiao Chi Yunpeng liu Shimelis Admassie Xinwen Peng State Key Laboratory of Pulp and Paper Engineering South China University of TechnologyGuangzhou510641GuangdongChina Hainan Key Laboratory of Storage&Processing of Fruits and Vegetables Agricultural Products Processing Research InstituteChinese Academy of Tropical Agricultural SciencesZhanjiangGuangdong524001China Department of Chemistry and Centre for Advanced 2D Materials(CA2DM)National University of Singapore 3 Science Drive 3 Singapore117543Singapore School of Materials Science and Hydrogen Energy Foshan UniversityFoshan528231GuangdongChina Department of Chemistry Addis Ababa UniversityPO BOX 1176Addis AbabaEthiopia

Photocatalysis has emerged as an effective approach to sustainably convert biomass into value-added ***_(2)is a promising nonprecious,efficient cocatalyst for photooxidation,which can facilitate the separation of photogenerated electron–holes,increase the reaction rates,and enhance photocatalytic *** this work,we synthesized a stable and efficient photocatalysis system of CoSe_(2)/g-C_(3)N_(4)through attaching CoSe_(2)on g-C_(3)N_(4)sheets,with a yield of 50.12%for the selective photooxidation of xylose to xylonic *** light illumination,the photogenerated electrons were prone to migrating from g-C_(3)N_(4)to CoSe_(2)due to the higher work function of CoSe_(2),resulting in the accelerated separation of photogenerated electron–holes and the promoted ***,this study reveals the unique function of CoSe_(2),which can significantly promote oxygen adsorption,work as an electron sink and accelerate the generation of ·O_(2)^(-),thereby improving the selectivity toward xylonic acid over other *** work provides useful insights into the design of selective photocatalysts by engineering g-C_(3)N_(4)for biomass high-value utilization.

关键词： Biomass photooxidation Hemicelluloses Xylose g-C_(3)N_(4) Xylonic acid

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Low-concentration atropine(0.01%)on quantitative contrast sensitivity function in Chinese children with myopia

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International Journal of Ophthalmology(English edition) 2025年第1期18卷 117-124页

作者： Yu-hao Ye Yi-Yong Xian Fang liu zhong-Lin Lyu Xing-Tao Zhou Jing Zhao Department of Ophthalmology and Optometry Eye&ENT Hospital of Fudan UniversityShanghai 200031China NHC Key Laboratory of Myopia(Fudan University) Key Laboratory of MyopiaChinese Academy of Medical SciencesShanghai 200000China Shanghai Research Center of Ophthalmology and Optometry Shanghai 200000China Shanghai Engineering Research Center of Laser and Autostereoscopic 3D for Vision Care(20DZ2255000).Shanghai 200030 China Division of Arts and Sciences NYU ShanghaiShanghai 200000China Center for Neural Science and Department of Psychology New York UniversityNew York 10012USA NYU-ECNU Institute of Brain and Cognitive Science NYU ShanghaiShanghai 200000China

AIM:To investigate the effect of 0.01%low-concentration atropine(LA)on quantitative contrast sensitivity function(qCSF)in children with ***:This paired case-control study included 90 eyes of 58 children who were sex-,age-,and refractionmatched and equally divided into two groups:the 0.01%LA group had undergone 6mo use of daily 0.01%atropine and control group was naïve to *** ophthalmic examinations and qCSF test without refractive correction were *** groups were compared in monocular and binocular qCSF parameters,including the area under logCSF,CSF acuity,and contrast sensitivity(CS)at 1.0-18.0 cycle per degree(cpd).RESULTS:In the monocular comparison,the CSF acuity of the LA group was significantly higher than that of the control group(7.58±5.51 vs 6.37±4.22 cpd,P<0.05).The subgroup analysis showed that in the 6-9y group,CSF acuity was significantly higher in the LA group than the control group(8.76±6.19 vs 6.54±4.25 cpd,P<0.05),and in the Female group,low refraction sphere group,and high refraction cylinder group,the CS at high spatial frequencies(12.0 and 18.0 cpd)were significantly higher in the LA group than in the control group(all P<0.05).In the binocular test,CSF acuity and CS at 12.0 cpd were significantly higher in the LA group than in the control group(10.95±7.00 vs 8.65±5.12 cpd;0.17±0.33 vs 0.06±0.16,respectively;both P<0.05).CONCLUSION:Use of LA may result in improved CS in children with early onset myopia.

关键词： low-concentration atropine myopia quantitative contrast sensitivity function Chinese children

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