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Effects of stocking density on the growth and immunity of Atlantic salmon salmo salar reared in recirculating aquaculture system(RAS)

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Journal of Oceanology and Limnology 2019年第1期37卷 350-360页

作者： WANG Yanfeng CHI Liang LIU Qinghua xiao Yongshuang MA Daoyuan xiao zhizhong XU Shihong LI Jun CAS Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology Center for Ocean Mega-Science Chinese Academy of Sciences College of Veterinary Medicine Qingdao Agricultural University

Atlantic salmon reared in recirculating aquaculture system(RAS) may lead to inappropriately high stocking density, because ?sh live in a limited space. Finding the suitable stocking density of Atlantic salmon reared in RAS is very important for RAS industry. In this paper, the in?uence of stocking density on growth and some stress related physiological factors were investigated to evaluate the ef fects of stocking density. The ?sh were reared for 220 days at ?ve densities(A: 24 kg/m^3; B: 21 kg/m^3; C: 15 kg/m^3; D: 9 kg/m^3 and E: 6 kg/m^3). The results show that 30 kg/m^3 might be the maximum density which RAS can af ford in China. The stocking densities under 30 kg/m^3 have no ef fect on mortality of Atlantic salmon reared in RAS. However, the speci?c growth rate(SGR), ?nal weight and weight gain in the high density group were signi?cantly lower than the lower density groups and middle density groups. Moreover, feed conversion rate(FCR) had a negative correlation with density. Plasma hormone T_3 and GH showed signi?cant decrease with the increase of the stocking density of the experiment. Furthermore, thyroid hormone(T 3), GH(growth hormone) activities were decreased with stocking density increase. However, plasma cortisol, GOT(glutamic oxalacetic transaminase) and GPT(glutamic pyruvic transaminase) activities were increase with stocking density increase. And the stocking density has no ef fects on plasma lysozyme of Atlantic salmon reared in RAS. These investigations would also help devise effcient ways to rear adult Atlantic salmon in China and may, in a way, help spread salmon mariculture in China.

关键词： stocking density growth physiological parameters Atlantic salmon RAS

CRPA感染死亡患者危险因素分析及NLR值对预后的评估

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智慧健康 2024年第17期10卷 17-20页

作者：李强薛东杰董珍珍肖志忠北京怀柔医院检验科北京101400 北京市怀柔区疾病预防控制中心微生物科北京101400

目的分析碳青霉烯类耐药铜绿假单胞菌(CRPA)感染患者的临床特征及预后危险因素。方法收集2018年1月—2023年5月本院收治的131例确定为CRPA感染患者的临床资料和实验室数据,根据首次从患者体内分离出CRPA后的30d内的生存状态,最终将患者... 详细信息

目的分析碳青霉烯类耐药铜绿假单胞菌(CRPA)感染患者的临床特征及预后危险因素。方法收集2018年1月—2023年5月本院收治的131例确定为CRPA感染患者的临床资料和实验室数据,根据首次从患者体内分离出CRPA后的30d内的生存状态,最终将患者分为死亡组(38例)和幸存组(93例)。对患者的危险因素进行分析,对NLR值发热预后进行评估。结果死亡组CRPA感染科室分布以ICU为主,幸存组CRPA感染科室分布以呼吸科、神经外科、ICU为主,呼吸科、神经外科、ICU分布情况,差异有统计学意义(P<0.05);在死亡组中,菌株对哌拉西林/他唑巴坦、头孢他啶以及头孢吡肟的耐药率呈现出较高水平;而在幸存组中,对环丙沙星、左氧氟沙星以及哌拉西林/他唑巴坦的耐药率相对较高。特别值得注意的是,哌拉西林/他唑巴坦以及头孢吡肟在两组之间的耐药率差异,经统计分析后显示差异有统计学意义(P<0.05);入住ICU、合并曲霉菌感染及CRPA血流感染是CRPA感染死亡的独立危险因素(P<0.05)。结论我院CRPA感染住院患者发生血流感染、合并曲霉菌感染以及入住ICU是高死亡率的独立危险因素,NLR值对预测CRPA感染患者预后有一定的临床应用价值。

关键词：碳青霉烯类耐药铜绿假单胞菌中性粒细胞与淋巴细胞比值受试者工作特征曲线危险因素

The ontogeny of acidic digestive tract in larval turbot Scophthalmus maximus based on H+/K+-ATPase and pepsinogen

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Journal of Oceanology and Limnology 2023年第3期41卷 1100-1108页

作者： Yanfeng WANG Shulan LIU Teng GUO Shihong XU Qinghua LIU zhizhong xiao Daoyuan MA Jun LI Liang CHI Key Laboratory of Experimental Marine Biology Centre for Ocean Mega-ScienceInstitute of OceanologyChinese Academy of SciencesQingdao 266071China Qingdao Agricultural University Qingdao 266109China Laboratory for Marine Biology and Biotechnology Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China Southern Marine Science and Engineering Guangdong Laboratory Guangzhou 511458China Conson Oriental(Yantai)Recirculating Aquaculture Technology Co. Ltd.Yantai 264000China

Acidic digestion is an important digestive process of marine *** fish stomach,two enzymes are involved in the secretion of hydrochloric acid(HCl)and proteomic digestion:H^(+)/K^(+)-ATPase and ***,the starting of digestive function in fish is still *** reveal the details of acidic digestion of turbot Scophthalmus maximus in early development,a 40 day of turbot larvae culture was *** H^(+)/K^(+)-ATPase gene from the turbot ***(smH^(+)/K^(+)-ATPase)was identified and *** on our previous discription on pepsinogen of turbot ***,we combined pepsinogen and H^(+)/K^(+)-ATPase and analyzed the mechanism of acidic digestion in *** show that the spatial and temporal expression profiles of H^(+)/K^(+)-ATPase agreed with pepsinogen A and C in turbot,indicating a synergetic action between H^(+)/K^(+)-ATPase and pepsinogen during the acidic digestion *** addition,the turbot juveniles showed a faster growth after the expressions of H^(+)/K^(+)-ATPase gene and pepsinogen gene,demonstrating that pepsin had a higher digestive efficiency,for which a compound diet should be provided to the fish from Day 22 *** study provided a reference for biology research and aquaculture of turbot and other marine fishes.

关键词： turbot Scophthalmus maximus acidic digestion H+/K+-ATPase pepsinogen

Effects of dissolved oxygen on intestinal bacterial community and immunity of Atlantic salmon Salmo salar

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Journal of Oceanology and Limnology 2023年第1期41卷 364-375页

作者： Shihong XU Yanfeng WANG Caixia GAO Sarath BABU V Jun LI Qinghua LIU zhizhong xiao Yingxuan XU Chunyan ZHAO Li LIN Liang CHI Department of Aquatic Animal Medicine College of FisheriesHuazhong Agricultural UniversityWuhan 430070China College of Veterinary Medicine Qingdao Agricultural UniversityQingdao 266109China Laboratory Animal and Comparative Medicine Team State Key Laboratory of Veterinary BiotechnologyHarbin Veterinary Research InstituteChinese Academy of Agricultural Sciences(CAAS)Harbin 150069China CAS Key Laboratory of Experimental Marine Biology Institute of OceanologyChinese Academy of SciencesQingdao 266071China Guangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl BreedingZhongkai University of Agriculture and EngineeringGuangzhou 510225China University College London London WC1E 6BTUnited Kingdom School of Marine Science and Engineering Qingdao Agricultural UniversityQingdao 266109China

Dissolved oxygen(DO)is one of most important factors which affect wide range physiologic features of including immune responses and intestinal bacterial ***,the underlying mechanisms remain *** address this question,the intestinal bacterial community compositions and the immune features of Atlantic salmon(Salmo salar)grown in recirculating aquaculture systems(RAS)were *** were reared under different DO saturation levels,e.g.,200% saturation named high group(H),100%saturation named control group(CK),and 60%saturation named lower group(L).Large variations in the operational taxonomic units(OTUs)frequency distribution for the intestinal bacterial community of Atlantic salmon were *** intestinal bacterial community of all groups was dominated mainly by three phyla,e.g.,***,and ***,Acinetobacter baumannii,an opportunistic pathogen of salmon was increased significantly in L *** further monitored the immunity features of fish under different DO *** results show that leucocyte number,cortisol level,the expressions of interleukin-1β(IL-1β),Toll-like receptor 4(TLR4),and nucleotide-binding oligomerization domain like protein 2(NOD2)were higher at significant levels in the L group than those in the other two ***4 and NOD2 are usually related with the bacterial infections;therefore,it is reasonable to believe that the stronger immune responses observed in the L group might be related with the higher abundance of *** in the inte stine of Atlantic ***,these findings demonstrated that low DO level may induce stronger immunity responses in Atlantic salmon.

关键词： Atlantic salmon dissolved oxygen(DO) immune responses microbiota intestine

碳量子点/TiO_(2)复合光催化剂的制备和应用进展

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化工技术与开发 2024年第6期53卷 47-52,61页

作者：赵斌沈谊豪周雨洁肖智中李静雯纪星竹于子轩王志有辽宁科技大学化学工程学院辽宁鞍山114051

本文主要综述了碳量子点/TiO_(2)复合光催化剂的制备方法,以及近5年碳量子点/TiO_(2)复合光催化剂在光催化降解有机污染物、杀菌、制氢及太阳能电池制备等方面的应用进展,以期为碳量子点/TiO_(2)复合光催化剂制备及应用的进一步研究提... 详细信息

本文主要综述了碳量子点/TiO_(2)复合光催化剂的制备方法,以及近5年碳量子点/TiO_(2)复合光催化剂在光催化降解有机污染物、杀菌、制氢及太阳能电池制备等方面的应用进展,以期为碳量子点/TiO_(2)复合光催化剂制备及应用的进一步研究提供参考。

关键词：碳量子点/TiO_(2) 光催化制备方法应用进展

一个全过程的城市设计实践——宜昌市中心城区景观规划

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新建筑 2003年第5期 67-69页

作者：陈韦胡跃平肖志中武汉市城市规划设计研究院武汉430014

以突出宜昌市中心城区整体景观环境和建立城市景观控制体系为目标,在国际征集方案的基础上,综合形成总体景观规划,并为老城区和黄柏河区的详细城市设计提供依据。通过城市景观宏观控制与具体可操作性设计的结合,实现宜昌市城市景观的全... 详细信息

以突出宜昌市中心城区整体景观环境和建立城市景观控制体系为目标,在国际征集方案的基础上,综合形成总体景观规划,并为老城区和黄柏河区的详细城市设计提供依据。通过城市景观宏观控制与具体可操作性设计的结合,实现宜昌市城市景观的全过程设计。

关键词：宜昌市城市设计城市景观总体景观规划中心城区

Priority-Based Mixed Assembly Supporting Differentiated Service for Optical Burst Switched Networks

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光学学报 2003年第S1期23卷 707-708页

作者： Jianxin Wang, Qingji Zeng, Hao chi, Shenghua Wang, Shilin xiao, zhizhong Zhang CBONT of Shanghai Jiaotong University, China 200030 2 Beijing Lyang Technology Co. Ltd. China 100085 CBONT of Shanghai Jiaotong University China 200030 Beijing Lyang Technology Co. Ltd. China 100085

In order to have a better support of differentiated service, we propose Priority-based mixed burst assembly, in which packets of different priorities are assembled in a burst with an assigned proportion, and the priorities are lined in an ascending order in a burst from head to tail. Simulation results show that the proposed scheme performs very well in terms of latency and packet loss probability.

关键词： for WDM IP Priority-Based Mixed Assembly Supporting Differentiated Service for Optical Burst Switched Networks into node OBS than PBMA been of QoS that length

Endoplasmic reticulum stress and autophagy in cerebral ischemia/reperfusion injury:PERK as a potential target for intervention

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Neural Regeneration Research 2025年第5期20卷 1455-1466页

作者： Ju Zheng Yixin Li Ting Zhang Yanlin Fu Peiyan Long xiao Gao Zhengwei Wang zhizhong Guan xiaolan Qi Wei Hong Yan xiao Key Laboratory of Endemic and Ethnic Diseases Ministry of Education&Key Laboratory of Medical Molecular Biology of Guizhou ProvinceGuizhou Medical UniversityGuiyangGuizhou ProvinceChina Guizhou Center for Disease Control and Prevention GuiyangGuizhou ProvinceChina Department of Histology and Embryology Guizhou Medical UniversityGuiyangGuizhou ProvinceChina Collaborative Innovation Center for Prevention and Control of Endemic and Ethnic Regional Diseases Co-constructed by the Province and Ministry GuiyangGuizhou ProvinceChina

Several studies have shown that activation of unfolded protein response and endoplasmic reticulum(ER)stress plays a crucial role in severe cerebral ischemia/reperfusion *** occurs within hours after cerebral ischemia,but the relationship between ER stress and autophagy remains *** this study,we established experimental models using oxygen-glucose deprivation/reoxygenation in PC12 cells and primary neurons to simulate cerebral ischemia/reperfusion *** found that prolongation of oxygen-glucose deprivation activated the ER stress pathway protein kinase-like endoplasmic reticulum kinase(PERK)/eukaryotic translation initiation factor 2 subunit alpha(e IF2α)-activating transcription factor 4(ATF4)-C/EBP homologous protein(CHOP),increased neuronal apoptosis,and induced ***,inhibition of ER stress using inhibitors or by si RNA knockdown of the PERK gene significantly attenuated excessive autophagy and neuronal apoptosis,indicating an interaction between autophagy and ER stress and suggesting PERK as an essential target for regulating *** autophagy with chloroquine exacerbated ER stress-induced apoptosis,indicating that normal levels of autophagy play a protective role in neuronal injury following cerebral ischemia/reperfusion *** from this study indicate that cerebral ischemia/reperfusion injury can trigger neuronal ER stress and promote autophagy,and suggest that PERK is a possible target for inhibiting excessive autophagy in cerebral ischemia/reperfusion injury.

关键词： apoptosis ATF4 autophagy C/EBP homologous protein cerebral ischemia/reperfusion injury eIF2α endoplasmic reticulum stress PERK

Decentralized stabilization of large-scale systems with time delays

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Decentralized stabilization of large-scale systems with time...