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Journal of Geographical Sciences 2024年第8期34卷 1537-1557页

作者： SUN Zhaohua li zhiqing CHEN li FAN Jiewei liU Ya State Key Laboratory of Water Resources Engineering and Management Wuhan UniversityWuhan 430072China Yellow River Water&Hydropower Development Group Co. Ltd.Jiyuan 459017HenanChina Changjiang River Scientific Research Institute Wuhan 430010China

Floodplain lakes are important water storage areas in lowland regions that often undergo geomorphologic evolution,and timely topographic data are generally *** this study,to assess the impacts of lakebed deformation on hydraulic performance in Dongting Lake,a set of semi-empirical methods was proposed to establish performance graphs(PGs)using only hydrological *** methods were used to evaluate the changes in water level,storage capacity,and flood detention ability in Dongting Lake caused by topographic adjustment after the Three Gorges Reservoir *** methods showed that PGs can effectively simulate the water level and outflow processes of Dongting Lake with Nash-Sutcliffe efficiency coefficients(NSEs)above 0.9.A comparison of the estimated water level and discharge using PGs from different periods suggested that bed erosion in Dongting Lake caused water level decreases of 0.18 m and 0.32 m during the flood and dry seasons,*** the magnitude of erosion at high elevations in the lake is small,the impacts of bed adjustment on the storage capacity and flood detention ability are not currently *** study showed that the hydraulic performance of a floodplain lake can be evaluated independently of topographic data under the condition of no reverse flows or negative watersurface slopes.

关键词： Dongting Lake hydraulic performance graph bed adjustment semi-empirical approach backwater effect

UV-B irradiation enhances the accumulation of beneficial glucosinolates induced by melatonin in Chinese kale sprout

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Horticultural Plant Journal 2024年第4期10卷 995-1006页

作者： Mengyu Wang Jiansheng Wang Yuhe Yang zhiqing li Ghazala Mustafa Yubo li lihong liu Zuofa Zhang Qiaomei Wang Zhejiang Academy of Agricultural Sciences HangzhouZhejiang 310021China Key Laboratory of Horticultural Plant Growth Development and Quality ImprovementMinistry of AgricultureDepartment of HorticultureZhejiang UniversityHangzhouZhejiang 310058China Department of Plant Sciences Quaid-i-Azam UniversityIslamabad 45320Pakistan

Cruciferous sprout is a new form of vegetable product rich in bioactive compounds,especially *** studies have focused on increasing the accumulation of glucosinolates in cruciferous sprouts by applying different chemical regulators,with a particular focus on their contribution to nutritional quality and health ***,the effects of melatonin and UV-B irradiation on glucosinolate biosynthesis remain *** this study,it was found that changes in melatonin concentrations significantly affected the contents of individual as well as total aliphatic and indolic *** 5μmol·L^(-1)melatonin was decided as the optimum concentration that could increase the content of beneficial glucosinolates including glucoraphanin and 4-methoxy glucobrassicin in Chinese kale ***,the enhancement of glucosinolate accumulation by melatonin treatment could be further amplified by UV-B ***,our results showed that R2R3-MYB transcription factor BoaMYB28 and BoaMYB51,which are central regulators of aliphatic and indolic glucosinolate biosynthesis respectively,were both involved in the regulation of glucosinolate biosynthesis by melatonin and UV-B ***,the expression of glucosinolate biosynthetic genes,including BoaCYP79F1,BoaCYP83A1,BoaSUR1,BoaUGT74B1,BoaCYP79B2,BoaCYP79B3,and BoaCYP83B1 participated in the formation of core structures and BoaFMOGS-OX5,BoaAOP2,BoaCYP81F2,and BoaIGMT1 involved in the sidechain modification of aliphatic and indolic glucosinolate,was regulated by melatonin or UV-B *** together,these findings provide a potential strategy for improving the nutritional quality and resistance of Chinese kale sprouts.

关键词： Melatonin Chinese kale sprouts UV-B Glucosinolates Nutritional quality Second metabolism

Research status, hot spots, difficulties and future development direction of microbial geoengineering

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Journal of Road Engineering 2024年第2期4卷 234-255页

作者： Yingxin Zhou zhiqing li Peng Zhang Qi Wang Weilin Pan Shuangjiao Wang Xiongyao Xie Department of Geotechnical Engineering College of Civil EngineeringTongji UniversityShanghai 200092China Yunnan Communications Investment&Construction Group Co. Ltd.Kunming 650103China Key Laboratory of Shale Gas and Geoengineering Institute of Geology and GeophysicsChinese Academy of SciencesBeijing 100029China College of Earth and Planetary Science University of Chinese Academy of SciencesBeijing 100049China Innovation Academy for Earth Science Chinese Academy of SciencesBeijing 100029China China Energy Technology and Economics Research Institute Beijing 102211China Cangzhou Qugang Expressway Construction Co. Ltd.Cangzhou 062450China Henan Yelu Expressway Construction Co. Ltd.Pingdingshan 467000China Beijing Zhongyan Tiandi Technology Co. Ltd.Beijing 100029China

Microbial geoengineering technology,as a new eco-friendly rock and soil improvement and reinforcement technology,has a wide application ***,this technology still has many deficiencies and is difficult to achieve efficient curing,which has become the bottleneck of large-scale field *** paper reviews the research status,hot spots,difficulties and future development direction microbial induced calcium carbonate precipitation(MICP)*** principle of solidification and the physical and mechanical properties of improved rock and soil are systematically *** solidification efficiency is mainly affected by the reactant itself and the external *** present,the MICP technology has been preliminarily applied in the fields of soil solidification,crack repair,anti-seepage treatment,pollution repair and microbial ***,the technology is currently mainly limited to the laboratory level due to the difficulty of homogeneous mineralization,uneconomical reactants,short microbial activity period and large environmental interference,incidental toxicity of metabolites and poor field *** directions include improving the uniformity of mineralization by improving grouting methods,improving urease persistence by improving urease activity,and improving the adaptability of bacteria to the environment by optimizing bacterial ***,the authors point out the economic advantages of combining soybean peptone,soybean meal and cottonseed as carbon source with phosphogypsum as calcium source to induce CaCO3.

关键词： Microbial geoengineering Microbial induced calcium carbonate precipitation Bacillus pasteurii Urease Phosphogypsum

Targeted DNA N^(6)-methyladenine editing by dCas9 fused to METTL4 in the lepidopteran model insect Bombyx mori

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Insect Science 2024年第2期31卷 646-650页

作者： Feng Chen Hao Guo Weiqun Lan Mingyi Zhou Wenjng Geng Guanwang Shen Ping lin Qingyou Xia Ping Zhao zhiqing li Integrative Science Center of Germplasm Creation in Western China(CHONGQING)Science City Biological Science Research CenterSouthwest UniversityChongqingChina Chongqing Key Laboratory of Sericultural Science Chongqing Engineering and Technology Research Center for Novel Silk MaterialsSouthwest UniversityChongqingChina

Dear Editor,N^(6)-Methyladenine(6mA)DNA modification is an important epigenetic mechanism with roles in regulating gene expression,nucleosome positioning,DNA damage repair,and cell cycle progression(Heyn&Esteller,2015;Luo et al.,2015;Boulias&Greer,2022).Despite progress in understanding the biological functions of 6mA,the contribution of individual 6mA installations on site-specific target genes is largely unknown,and therefore deciphering the molecular mechanism of 6mA in target gene expression has been difficult.

关键词： adenine fused Bombyx

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论北方海航道开发前景:气候、海运与破冰船

海洋经济 2024年第2期14卷 32-42页

作者：李志庆黑龙江省社会科学院哈尔滨150028

随着全球气候不断变暖,北极冰川融化加剧,北方海航道海域冰层日趋缩小和变薄,为北方海航道开发提供了历史性机遇。全球化趋势下,国际货物贸易飞速发展,国际海运尤其是欧亚大陆间海运活动昌盛,传统南方航道面临压力,为北方海航道开发提... 详细信息

随着全球气候不断变暖,北极冰川融化加剧,北方海航道海域冰层日趋缩小和变薄,为北方海航道开发提供了历史性机遇。全球化趋势下,国际货物贸易飞速发展,国际海运尤其是欧亚大陆间海运活动昌盛,传统南方航道面临压力,为北方海航道开发提供了现实需求。破冰船自出现以来,不断迭代升级,在动力、船体设计、船体材料、辅助设备等方面飞速发展,破冰能力不断提高,为北方海航道开发提供了技术可能。近年来北方海航道的开发取得飞速发展,而且俄罗斯仍在加大对北方海航道的开发力度,但北方海航道开发也面临国际合作环境恶化、资金投入大、经济性不强等问题。整体而言,在气候变暖、海运需求旺盛和破冰船技术不断发展的大背景下,长期来看,北方海航道开发的前景仍十分广阔。

关键词：北方海航道气候变暖海运破冰船俄罗斯

基于遥感影像的上荆江典型洲滩植被适宜区分布特征研究

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水利学报 2024年第2期55卷 238-252页

作者：袁景耀孙昭华李芷晴周炜兴陈立武汉大学水资源工程与调度全国重点实验室湖北武汉430072

江心洲滩是长江中游河漫滩重要组成部分,厘清洲滩上植被生长适宜区域的分布特征,以及这些区域所应满足的淹没频率临界条件,是生态护滩规划设计和三峡建库后洲滩植被变化预测的重要前提。本文选取上荆江关洲和突起洲分别作为卵石夹沙和... 详细信息

江心洲滩是长江中游河漫滩重要组成部分,厘清洲滩上植被生长适宜区域的分布特征,以及这些区域所应满足的淹没频率临界条件,是生态护滩规划设计和三峡建库后洲滩植被变化预测的重要前提。本文选取上荆江关洲和突起洲分别作为卵石夹沙和沙质型洲滩的典型代表,基于Landsat遥感影像提取了1986—2002年的长时序洲滩淹水范围和植被信息,改进并利用水文特性与影像数据相结合的方法计算了滩面上不同位置的淹没频率和植被频率,分析了二者间的响应规律,确定了植被生长适宜区对应的淹没率条件。研究结果表明:两类洲滩上都存在低、高植被频率区像元相对集中,而中等植被频率区像元较为分散的规律,根据植被频率分布特征,可将滩面区域划分为高频次植被覆盖区、中频次植被覆盖区、过渡区和不适宜区,各区之间的植被频率临界值分别为80%、50%、20%;滩面不同位置的淹没频率是影响植被频率的主导因素,从长时期平均来看两类洲滩上的淹没频率-植被频率响应关系符合统一的Logistic曲线,根据该曲线可确定出各区域的临界淹没条件分别为年均18 d、43 d、92 d。在年均淹没天数大于92 d的区域内,随着淹没天数增加,植被频率迅速减少至10%以下。本文的方法和认识可为三峡建库后坝下游洲滩植被演替趋势预测提供参考。

关键词：上荆江江心洲植被遥感影像淹没频率临界条件

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秋石斛胚性愈伤组织诱导及再生体系的建立

热带作物学报 2024年第6期45卷 1175-1183页

作者：李志晴李亚梅李崇晖尹俊梅海南大学热带农林学院海南海口570228 中国热带农业科学院热带作物品种资源研究所/农业农村部华南作物基因资源与种质创制重点实验室/海南省热带作物资源遗传改良与创新重点实验室海南海口571101 海南省热带观赏植物种质创新利用工程技术研究中心海南儋州571737 中国热带农业科学院三亚研究院海南三亚572330 中国热带农业科学院海口实验站海南海口571101

秋石斛(Phalaenopsis-hybrid Dendrobium)花姿优美、花色丰富,是热区重要观赏植物,具有极高的产业价值。本研究以秋石斛杂交种迷你(*** Victoria)、水蜜桃(***)、三亚阳光(*** Hiasakul)原球茎和无菌苗幼嫩茎段为外植体,探讨不同植物生... 详细信息

秋石斛(Phalaenopsis-hybrid Dendrobium)花姿优美、花色丰富,是热区重要观赏植物,具有极高的产业价值。本研究以秋石斛杂交种迷你(*** Victoria)、水蜜桃(***)、三亚阳光(*** Hiasakul)原球茎和无菌苗幼嫩茎段为外植体,探讨不同植物生长调节剂组合对不同品种秋石斛胚性愈伤诱导、不定芽分化及植株再生的影响。结果表明:在胚性愈伤组织诱导阶段,原球茎和生长2个月的幼苗茎段作为外植体诱导效果最佳,暗培养30 d即可获得胚性愈伤。3个品种的愈伤组织诱导最佳培养基是以MSD(MS+30 g/L葡萄糖+1 g/L花宝1号+8 g/L琼脂)为基本培养基,添加0.5 mg/L KT和0.2~0.5 mg/L 2,4-D。迷你、水蜜桃、三亚阳光的胚性愈伤诱导率最高分别为31.11%、22.78%和50%,其中迷你品种仅需15 d即可获得胚性愈伤。最佳胚性愈伤增殖培养基为MSD+0.5 mg/L IBA+0.5 mg/L KT,胚性愈伤状态保持良好且不发生分化,增殖倍数0.9。最佳分化培养基为1/2 MS+0.5 mg/L IBA+0.15 mg/L KT,在光照条件下,接种后的胚性愈伤组织能够快速诱导形成芽点,30 d内分化形成2~3片叶的幼苗,相同培养基中保持60 d能够自行生根获得完整再生苗。最佳生根培养基为1/2 MS+30 g/L蔗糖+0.5 mg/L NAA+8 g/L琼脂,生根率为100%,平均生根数5条,根长约1.3 cm。本研究所使用的胚性愈伤组织诱导、增殖和分化培养基具有一定的广谱性,可为后续秋石斛胚再生途径及分子育种研究奠定基础。

关键词：秋石斛茎段胚性愈伤植株再生

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微生物岩土工程技术的过去、现在与未来

工程地质学报 2024年第1期32卷 236-264页

作者：王双娇李志清田怡帆李燕明周应新李丹丹中国科学院地质与地球物理研究所页岩气与地质工程重点实验室北京100029 中国科学院大学北京100049 中国科学院地球科学研究院北京100029 中冶华亚建设集团有限公司武汉430083 北京中岩天地科技有限公司北京100102 云南省交通投资建设集团有限公司昆明650100 云南会巧高速公路有限公司会泽654200

微生物岩土工程技术作为一种新兴的生态友好型岩土体改良加固技术,应用前景广阔。但限于理论水平和研究手段,该技术仍存在较多不足,难以实现高效固化,由此成为大规模现场应用的瓶颈。而提升固化效率的关键在于明确其作用原理和影响机制... 详细信息

微生物岩土工程技术作为一种新兴的生态友好型岩土体改良加固技术,应用前景广阔。但限于理论水平和研究手段,该技术仍存在较多不足,难以实现高效固化,由此成为大规模现场应用的瓶颈。而提升固化效率的关键在于明确其作用原理和影响机制。文章梳理了微生物诱导碳酸钙沉积技术(MICP)的研究现状,系统归纳了固化原理和改良岩土体的物理力学特性,并分析得出固化效率主要受到反应物自身和外部环境两方面的影响。当前MICP技术已初步应用于土体固化、裂缝修复、防渗处理、污染修复及微生物水泥等领域,但由于矿化难以均匀、反应物不经济、微生物及脲酶活性期短且受环境干扰大、代谢产物附带毒性、现场应用性差,该技术目前主要限于实验室水平。作者分别提出了可能的突破与改进方向,并结合实验室成果指出豆粕进行菌体扩培和脲酶供给的碳源优势,以及将磷石膏作为现场钙源的环保性和经济性,以期为从事微生物岩土工程研究与技术开发的人员提供参考。

关键词：微生物岩土工程微生物地质工程微生物诱导碳酸钙沉积巴氏芽孢杆菌脲酶磷石膏

肠道菌群和黏蛋白2糖基化修饰的互作机制及其营养调控

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动物营养学报 2024年第9期36卷 5523-5534页

作者：王晶晶杨凯李志青马晓康湖南农业大学动物科学技术学院长沙410128

黏蛋白(Muc)2是组成肠道黏液屏障的主要成分,糖基化修饰是Muc2发挥作用的决定性因素,同时肠道共生菌是影响糖基化程度的重要影响因素。糖基化修饰决定Muc2的构象和理化特性、保护Muc2的完整性并且影响肠道共生菌的定植,反过来肠道共生... 详细信息

黏蛋白(Muc)2是组成肠道黏液屏障的主要成分,糖基化修饰是Muc2发挥作用的决定性因素,同时肠道共生菌是影响糖基化程度的重要影响因素。糖基化修饰决定Muc2的构象和理化特性、保护Muc2的完整性并且影响肠道共生菌的定植,反过来肠道共生菌可以调节Muc2等Muc的糖基化修饰。本文将重点介绍肠道共生菌和Muc2糖基化修饰的互作机制及其营养调控,旨在为仔猪肠道Muc2糖基化修饰的营养干预及相关研究提供参考。

关键词：黏蛋白2 肠道屏障营养调控肠道菌群糖基化修饰

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锂离子电池硅锗复合负极材料的研究进展

材料导报 2024年第S1期38卷 10-15页

作者：张磊曲昌宇李治庆董昱免高培波山东理工大学物理与光电工程学院山东淄博255000

随着电动汽车和储能技术的飞速发展,目前商用锂离子电池以石墨为负极,比容量低,已无法满足储能需求,因此,开发高比能量的锂离子电池迫在眉睫。采用高比容量的硅锗基负极材料替代传统石墨,可以协同发挥二者优势,提高比容量的同时获得高... 详细信息

随着电动汽车和储能技术的飞速发展,目前商用锂离子电池以石墨为负极,比容量低,已无法满足储能需求,因此,开发高比能量的锂离子电池迫在眉睫。采用高比容量的硅锗基负极材料替代传统石墨,可以协同发挥二者优势,提高比容量的同时获得高倍率性能,是制备高能锂离子电池的有效途径。然而,硅锗基负极材料在嵌锂过程中的体积膨胀和结构变化是实际应用过程中面临的关键问题。通过对硅锗基负极材料进行结构设计和组分优化,可以有效改善其电化学性能,对制备高能锂离子电池具有重要意义。本文从结构设计、组分优化及电化学性能方面对现阶段硅锗基负极材料进行了系统综述,并对该类负极材料未来发展进行了展望。

关键词：硅锗锂离子电池负极材料高比容量协同效应