Targeted DNA N^(6)-methyladenine editing by dCas9 fused to METTL4 in the lepidopteran model insect Bombyx mori

作     者：Feng Chen Hao Guo Weiqun Lan Mingyi Zhou Wenjng Geng Guanwang Shen Ping Lin Qingyou Xia Ping Zhao Zhiqing Li 

作者机构：Integrative Science Center of Germplasm Creation in Western China(CHONGQING)Science CityBiological Science Research CenterSouthwest UniversityChongqingChina Chongqing Key Laboratory of Sericultural ScienceChongqing Engineering and Technology Research Center for Novel Silk MaterialsSouthwest UniversityChongqingChina 

出 版 物：《Insect Science》 (昆虫科学（英文版）)

年 卷 期：2024年第31卷第2期

页      面：646-650页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 

基　　金：supported by National Key Research and Development Program of China(No.2022YFD1201600) National Natural Science Foundation of China(Nos.32030103 and 32172798) Natural Science Foundation of Chongqing(No.cstc2020jcyj-cxttX0001) 

主　　题：adenine fused Bombyx 

摘      要：Dear Editor,N^(6)-Methyladenine(6mA)DNA modification is an important epigenetic mechanism with roles in regulating gene expression,nucleosome positioning,DNA damage repair,and cell cycle progression(Heyn&Esteller,2015;Luo et al.,2015;Boulias&Greer,2022).Despite progress in understanding the biological functions of 6mA,the contribution of individual 6mA installations on site-specific target genes is largely unknown,and therefore deciphering the molecular mechanism of 6mA in target gene expression has been difficult.