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Chinese Chemical Letters 2020年第12期31卷 3131-3134页

作者： Li Zeng zhipeng su Xingyi Li Shuai Shi Institute of Biomedical Engineering School of Ophthalmology&Optometry and Eye HospitalWenzhou Medical UniversityWenzhou 325027China Department of Neurosurgery First Affiliated Hospital of Wenzhou Medical UniversityWenzhou 325000China

To explore the effects of microenvironmental adjustments on fluorescence,a pH-sensitive nanocomposite system based on fluorescence resonance energy transfer(FRET)was constructed.The model system included a modified triblock copolymer(polyhistidine-b-polyethylene glycol-b-polycaprolactone)and gold nanoparticles.A near-infrared dye was used as the donor,and spectrally matched gold nanorods,attached after C-terminus modification with α-lipoic acid,were used as the receptor to realize control of the FRET effect over the fluorescence intensity for two polymer configurational changes(i.e.,"folded"and"stretched"states)in response to pH.After synthesis and characterization,we investigated the self-assembly behavior of the system.Analysis by quartz crystal microbalance revealed the pH sensitivity of the polymer,which exhibited"folding"and"stretching"states with changes in pH,providing a structural basis for the FRET effect.Fluorescence spectrophotometry investigations also revealed the regulatory impact of the assembled system on fluorescence.

关键词： Fluorescence resonance energy transfer pH response Self-assembled nanoparticle Au nanorod Block copolymer

Hierarchically porous zeolites synthesized with carbon materials as templates

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Frontiers of Chemical Science and Engineering 2021年第6期15卷 1444-1461页

作者： Huan Wang Guo Du Jiaqing Jia Shaohua Chen zhipeng su Rui Chen Tiehong Chen Institute of New Catalytic Materials Science School of Materials Science and EngineeringKey Laboratory of Advanced Energy Materials Chemistry(MOE)Nankai UniversityTianjin300350China

Hierarchically porous zeolites are promising candidates in catalytic conversion of relatively bulky molecules,and their syntheses have attracted significant attention.From both industrial and scientific perspectives,different carbon materials have been widely employed as hard templates for the preparation of hierarchically porous zeolites during the past two decades.In this review,the progress in synthetic strategies using carbon materials as templates is comprehensively summarized.Depending on the affinity between the carbon templates and zeolite precursors,the substantial strategies for synthesizing hierarchical zeolites are introduced in direct templates and indirect templates.Direct templates methods,by which the carbon materials are directly mixed with precursors gel as hard templates,are first reviewed.Then,we discuss the indirect templates method(crystallization of carbon-silica composites),by which the carbon is produced by in situ pyrolysis of organic-inorganic precursors.In addition,the technique of encapsulating metal species into zeolites crystals with the assistance of carbon templates is also discussed.In the conclusion part,the factors affecting the synthesis of carbon-templated hierarchically porous zeolites are remarked.This review is expected to attract interest in the synthesis strategies of hierarchically porous zeolites,especially cost-effective and large-scale production methodologies,which are essential to the industrial application of hierarchical zeolites.

关键词： hierarchical zeolites carbon materials direct templates indirect templates carbon-silica composites

基于养分平衡原理的温室番茄沼液替代氮肥适宜模式及评价

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中国农业大学学报 2024年第2期29卷 77-90页

作者：郑健宿智鹏刘俊彦王燕兰州理工大学能源与动力工程学院兰州730050 甘肃省生物质能与太阳能互补供能系统重点实验室兰州730050 西北低碳城镇支撑技术协同创新中心兰州730050

为探索沼液替代氮肥的适宜模式,以番茄为供试材料,首先根据作物养分平衡原理确定试验种植番茄的施氮量为374.8 kg/hm^(2),并基于该施氮量开展沼液替代氮肥适宜模式试验和评价分析。试验设置纯沼液处理(CF1)、纯氮肥处理(CF2)、沼液替代5... 详细信息

为探索沼液替代氮肥的适宜模式,以番茄为供试材料,首先根据作物养分平衡原理确定试验种植番茄的施氮量为374.8 kg/hm^(2),并基于该施氮量开展沼液替代氮肥适宜模式试验和评价分析。试验设置纯沼液处理(CF1)、纯氮肥处理(CF2)、沼液替代50%(T1)、35%(T2)、25%(T3)、15%(T4)6个处理,分析沼液替代氮肥对番茄农艺性状、产量和品质的影响,并对其农艺性状生长潜力及最大生长速率进行Logistic曲线分析,同时选取番茄可溶性糖、可滴定酸、可溶性固形物、维生素C、硝酸盐和含水量作为评价指标,运用TOPSIS法、灰色关联分析法、对称交互熵多属性排序法和VIKOR法4种单一评价方法进行多属性决策评价,再采用均值法、模糊Borda法和Copeland法对4种单一评价结果进行组合评价,由此构建番茄品质综合效益评价体系。研究表明:T3处理番茄生长速率最大,呈现最优的番茄农艺性状;Logistic曲线的生长潜力及最大生长速率表现为T3>T4>T2>T1;T2处理产量最大(4.72 kg/株),T3次之(4.55 kg/株);随着沼液替代氮肥比例的增加,可溶性糖、可溶性固形物含量呈现增长趋势,硝酸盐含量呈现下降趋势,可滴定酸、维生素C和果实含水率呈现开口向下的抛物线趋势,T2处理为抛物线最大值;4种单一评价方法得到结果存在差异,3种组合评价法结果均得到T2处理(沼液替代35%氮肥)组合评价得分最高,尽管T3处理(沼液替代25%氮肥)时番茄的农艺性状(株高、茎粗及干物质累积质量)及最大生长速率表现最优,但T2处理(沼液替代35%氮肥)可获得最适宜的番茄果实产量、品质及评价结果。

关键词：番茄沼液氮肥养分平衡品质组合评价

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自体髂骨移植联合钛板胸骨重建一例

中国胸心血管外科临床杂志 2024年第2期31卷 329-332页

作者：杨博李斌蒋鹏张少博任美玉苏志鹏兰州大学第二医院胸外科兰州大学第二临床医学院兰州730030

胸骨是胸廓的枢纽性成分,上部和两侧分别与锁骨及肋骨相连,在保护胸壁稳定性方面发挥重要作用。胸骨切除通常会产生大的节段性胸壁缺损,导致胸壁漂浮,必须进行胸骨重建。本文报道1例62岁胸腺鳞状细胞癌伴胸骨转移男性患者,完善相关检查... 详细信息

胸骨是胸廓的枢纽性成分,上部和两侧分别与锁骨及肋骨相连,在保护胸壁稳定性方面发挥重要作用。胸骨切除通常会产生大的节段性胸壁缺损,导致胸壁漂浮,必须进行胸骨重建。本文报道1例62岁胸腺鳞状细胞癌伴胸骨转移男性患者,完善相关检查,排除手术禁忌后行胸腺切除、胸骨肿瘤切除并同期行自体髂骨移植联合钛板胸骨重建术。术后随访6个月,患者呼吸运动功能正常,胸廓外观良好。

关键词：胸骨重建自体髂骨移植钛板病例报告

The Long Noncoding RNA-H19 and Pituitary Adenomas

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The Long Noncoding RNA-H19 and Pituitary Adenomas

第十六届中国医师协会神经外科医师年会

作者： Zerui Wu zhipeng su Zhe Bao Wu Department of Neurosurgery First Affiliated Hospital of Wenzhou Medical University

Objective:To investigate the role of H19 on pituitary adenomas and its underlying mechanism in vitro and in vivo..Methods:Pituitary tumor tissues were obtained from pituitary tumor patients who underwent surgery between 2015 and 2020 at the Department of Neurosurgery,Ruijin Hospital of Shanghai Jiaotong University and Department of Neurosurgery,First Affiliated Hospital of Wenzhou Medical University;The Cell Counting Kit-8(CCK-8) assay was used to test cell viability;Real-time quantitative PCR was used to test the level of H19 and miR-93a;Western blot experiments were conducted to test the ATG7 expression level;Luciferase reporter assay and RNA immunoprecipitation were used to ensure the combination of H19 and miR-93a;Xenograft experiments were used to test the effect of H19 and miR-93a in vivo.Results:H19 inhibited tumor growth and had a synergistic effect with DA treatment on prolactinomas in vitro and in vivo.Mechanistically,H19 promoted ATG7 expression in pituitary tumor cells by inhibiting miR-93a expression.In addition,a potential binding site between miR-93 and H19 was confirmed,and low expression of miR-93 was previously found in DA-resistant prolactinomas.Furthermore,we showed that miR-93a regulates ATG7 expression by targeting ATG7 mRNA.Conclusion:Our study has identified the role of the H19-miR-93-ATG7 axis in tumor growth and DA treatment of pituitary tumors,which may be a potential therapeutic target for human pituitary tumors..

关键词： lncRNA H19 pituitary tumor miRNA-93 ATG7

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钛合金微槽超声螺线辅助铣磨加工试验研究

机械工程学报 2024年第9期60卷 5-12页

作者：苏志朋梁志强李娟王飞魏正义刘月红金惟薇马悦殷振王西彬北京理工大学机械与车辆学院北京100081 内蒙古北方重工业集团有限公司包头014030 苏州科技大学机械工程学院苏州215009

微铣削是实现航空微小零件精密加工的主要方法,但是受尺寸效应影响,使用该工艺加工钛合金等塑性高且强度大的材料时,易产生严重的切削变形以及毛刺等缺陷,影响微小零件加工质量。针对钛合金微槽加工过程中出现塑性变形大以及毛刺损伤严... 详细信息

微铣削是实现航空微小零件精密加工的主要方法,但是受尺寸效应影响,使用该工艺加工钛合金等塑性高且强度大的材料时,易产生严重的切削变形以及毛刺等缺陷,影响微小零件加工质量。针对钛合金微槽加工过程中出现塑性变形大以及毛刺损伤严重的问题,提出超声螺线辅助铣磨加工方法,将平面加工轨迹转变为三维螺线,进而实现微铣磨工具的端面和圆柱面与工件周期性分离,降低微铣磨力,改善微槽加工质量。开展超声辅助钛合金微槽铣磨加工仿真与试验研究,对比分析不同超声振动方式对微槽毛刺尺寸、表面质量、分形维数以及微铣磨力的影响,结果表明,相比无超声辅助、纵扭超声辅助以及切向超声辅助加工方法,超声螺线辅助加工得到毛刺尺寸最小,表面粗糙度最低,分形维数最大,微铣磨力最小。钛合金微槽加工质量得到了提高。验证了超声螺线辅助加工方法在钛合金微槽加工中的优势。

关键词：超声振动微铣削微磨削聚晶金刚石工具钛合金

Calpeptin Increases Tumor Cell Growth and Invasion of Glioblastoma Multiforme by Inhibiting the Cleavage of Filamin A

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Calpeptin Increases Tumor Cell Growth and Invasion of Gliobl...

2019年浙江省神经外科学学术大会

作者： Qun Li Lin Cai zhipeng su Xianghe Lu THE FIRST AFFILIATED HOSPITAL OF WEN ZHOU MEDICAL UNIVERSITY

Objective Filamin A (FLNA) was the most widely expressed isoform of filamin in mammalian tissues.It could be hydrolyzed by Calpain at the hinge 1 and 2 domains,producing a 90-kDa carboxyl-terminal fragment (FLNACT/ABP90).Calpeptin was a chemical inhibitor of Calpain,which could inhibit this effect.It had been shown that ABP90 acted as a transporting transcription factor which was involved in mediating cell signaling in the cell nucleus.

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MicroRNA-93 mediates cabergoline-resistance by targeting ATG7 in prolactinoma

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MicroRNA-93 mediates cabergoline-resistance by targeting ATG...

2018年浙江省神经外科学学术年会

作者： Zerui Wu Lin Cai Jianglong Lu zhipeng su First Affiliated Hospital of Wenzhou Medical University

Objective Our previous study found that expression of miRNA-93 increased obviously in DA-resistant prolactinomas, however, the role of miRNA-93 in DA-resistance was largely unexplored. This study is to find regulatory mechanism underlyingmiRNA-93 inhibits CAB efficiency.Methods The expression of miRNAs in human pituitary tumors was analyzed by microarray and RT-PCR. Cell viability and proliferation was determined by MTS assays.

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RBM17 controls apoptosis and proliferation to promote Glioma progression

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RBM17 controls apoptosis and proliferation to promote Glioma...

2018年浙江省神经外科学学术年会

作者： Chengde wang Jianglong Lu zhipeng su Qun Li The first affiliated hospital of wenzhou medical university

Objective The splicing factor SPF45 (RBM17) is a well-known component of the spliceosome that is involved in alternative splicing. RBM17 is frequently overexpressed in many tumors and plays a crucial role in cancer progression and drug resistance. However, the role of RBM17 in the development of glioma has not been thoroughly elucidated to date.

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Construction of lentiviral vector encoding human vascular endothelial growth factor165 gene and transfection of human neural stem cells

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Construction of lentiviral vector encoding human vascular en...

2014浙江省神经外科学学术年会

作者： Yu Zhang zhipeng su Huajun Jing Xiaobing Wang Qichuan Zhuge Department of Neurosurgery First Affiliated Hospital of Wenzhou Medical University

Background:Despite neural stem cells(NSCs)transplantation can promote function recovery of intracerebral hemorrhage(ICH)rats,low survival rate of grafted NSCs in ICH rats is a grave concern.Recent studies show that vascular endothelial growth factor(VEGF)play key roles in proliferation,differentiation and survival of NSCs.Methods:We construct a lentiviral vector carrying VEGF165 gene,which was used to transfect human NSCs,so as to produce therapeutic seed cells.We amplified VEGF165gene from human breast cancer cell line MCF-7 using RT-PCR and cloned the target gene into the pCDH-CMV-MCS-EF1-copGFP,a lentiviral expressing plasmid.After transformation,Enzyme digestion got a correct length of VEGF165 gene,DNA sequencing analysis confirmed that VEGF165 gene sequence was exactly same as that reported by GeneBank.And then transfected the recombinant lentivirus produced by 293T cells after cotransfection of pCDH-VEGF165 and packaging plasmids into passage fourth human NSCs.Transfection efficiency is 63%,tested by flow cytometry(FCM),VEGF165 mRNA and protein stable expression in transfected NSCs(VEGF165-NSCs)from 1 week after infection,and continue to 2 months.Results:Proliferation and VEGF secretion ability of NSCs significantly increased in pCDH-VEGF165 transfection group compared with empty vector transfected group and bland control group.pCDH-VEGF165 transfected NSCs still keep their characteristics and multiplex differentiation activity.Conclusions:Our results indicate that we have constructed the lentiviral vector carrying human VEGF165 gene successfully and accomplished transfection of human NSCs,which may be used as a gene therapeutic tool to promote proliferation,differentiation and survival of NSCs in treatment of ICH.

关键词： VEGF Lentiviral vector Neural stem cell（NSC） Transfection