The Long Noncoding RNA-H19 and Pituitary Adenomas
作者单位：Department of NeurosurgeryFirst Affiliated Hospital of Wenzhou Medical University
会议名称：《第十六届中国医师协会神经外科医师年会》
会议日期：2022年
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
关 键 词：lncRNA H19 pituitary tumor miRNA-93 ATG7
摘 要：Objective:To investigate the role of H19 on pituitary adenomas and its underlying mechanism in vitro and in vivo..Methods:Pituitary tumor tissues were obtained from pituitary tumor patients who underwent surgery between 2015 and 2020 at the Department of Neurosurgery,Ruijin Hospital of Shanghai Jiaotong University and Department of Neurosurgery,First Affiliated Hospital of Wenzhou Medical University;The Cell Counting Kit-8(CCK-8) assay was used to test cell viability;Real-time quantitative PCR was used to test the level of H19 and miR-93a;Western blot experiments were conducted to test the ATG7 expression level;Luciferase reporter assay and RNA immunoprecipitation were used to ensure the combination of H19 and miR-93a;Xenograft experiments were used to test the effect of H19 and miR-93a in ***:H19 inhibited tumor growth and had a synergistic effect with DA treatment on prolactinomas in vitro and in ***,H19 promoted ATG7 expression in pituitary tumor cells by inhibiting miR-93a *** addition,a potential binding site between miR-93 and H19 was confirmed,and low expression of miR-93 was previously found in DA-resistant ***,we showed that miR-93a regulates ATG7 expression by targeting ATG7 ***:Our study has identified the role of the H19-miR-93-ATG7 axis in tumor growth and DA treatment of pituitary tumors,which may be a potential therapeutic target for human pituitary tumors..