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Nuclear Science and Techniques 2024年第1期35卷 18-33页

作者： Xi-Guang Cao Yan-Long Chang Kai Chen Emilio Ciuffoli li-Min Duan Dong-liang Fang Chao-Song Gao Surja K.Ghorui Peng-Chong Hu Qiang Hu Si-Yuan Huang Ze-Yu Huang Lei Lang Yu-Lan li zhi-jie li Tian-Yu liang Jun liu Chen-Gui Lu Fa-Tai Mai Yuan Mei Hao Qiu Xiang-Ming Sun Xiao-Xing Tang Hu-lin Wang Qian-Ming Wang Le Xiao Mu-Yun Xiao Jian-Yu Xin Nu Xu Peng Yang Yi-Chen Yang Zhen Yang Zong-Yang Yu Dong-liang Zhang Jun-Wei Zhang Cheng-Xin Zhao Dou Zhu NνDEx-100 collaboration Shanghai Advanced Research Institute Chinese Academy of SciencesShanghai 201210China Lanzhou University Lanzhou 730000China Central China Normal University Wuhan 430079China Institute of Modern Physics Chinese Academy of SciencesLanzhou 730000China Shanghai Institute of Applied Physics Chinese Academy of SciencesShanghai 201800China Lawrence Berkeley National Laboratory BerkeleyCA 94720USA North China University of Water Resources and Electric Power Zhengzhou 450045China 不详

Observing nuclear neutrinoless double beta (0vββ) decay would be a revolutionary result in particle physics.Observing such a decay would prove that the neutrinos are their own antiparticles,help to study the absolute mass of neutrinos,explore the origin of their mass,and may explain the matter-antimatter asymmetry in our universe by lepton number violation.We propose developing a time projection chamber (TPC) using high-pressure ^(82)SeF_(6) gas and Topmetal silicon sensors for readout in the China Jinping Underground Laboratory (CJPL) to search for neutrinoless double beta decay of82Se,called the NvDEx experiment.Besides being located at CJPL with the world’s thickest rock shielding,NvDEx combines the advantages of the high Qββ(2.996 MeV) of82Se and the TPC’s ability to distinguish signal and background events using their different topological characteristics.This makes NvDEx unique,with great potential for low-background and high-sensitivity 0 vββsearches.NvDEx-100,a NvDEx experiment phase with 100 kg of SeF_(6)gas,is being built,with plans to complete installation at CJPL by 2025.This report introduces 0 vββ physics,the NvDEx concept and its advantages,and the schematic design of NvDEx-100,its subsystems,and background and sensitivity estimation.

关键词： Neutrinoless double beta decay Time projection chamber ^(82)SeF_(6) China Jinping Underground Laboratory

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Celastrol mitigates inflammation in sepsis by inhibiting the PKM2-dependent Warburg effect

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Military Medical Research 2023年第1期10卷 17-31页

作者： Piao Luo Qian Zhang Tian-Yu Zhong Jia-Yun Chen Jun-Zhe Zhang Ya Tian liu-Hai Zheng Fan Yang ling-Yun Dai Chang Zou zhi-jie li Jing-Hua liu Ji-Gang Wang Artemisinin Research Center and Institute of Chinese Materia MedicaChinese Academy of Chinese Medical SciencesBeijing 100700China Guangdong Provincial Key Laboratory of New Drug Screening School of Pharmaceutical SciencesSouthern Medical UniversityGuangzhou 510515China Laboratory Medicine the First Affiliated Hospital of Gannan Medical UniversityGanzhou 341000JiangxiChina Department of Geriatric Medicine Shenzhen People’s Hospitalthe Second Clinical Medical CollegeJinan University and the First Affiliated HospitalSouthern University of Science and TechnologyShenzhen 518020GuangdongChina Guangdong Provincial Key Laboratory of Proteomics School of Basic Medical SciencesSouthern Medical UniversityGuangzhou 510515China Center for Reproductive Medicine Dongguan Maternal and Child Health Care HospitalSouthern Medical UniversityDongguan 523125GuangdongChina Central People’s Hospital of Zhanjiang Zhanjiang 524037GuangdongChina

Background: Sepsis involves life-threatening organ dysfunction and is caused by a dysregulated host response to infection. No specific therapies against sepsis have been reported. Celastrol(Cel) is a natural anti-inflammatory compound that shows potential against systemic inflammatory diseases. This study aimed to investigate the pharmacological activity and molecular mechanism of Cel in models of endotoxemia and sepsis.Methods: We evaluated the anti-inflammatory efficacy of Cel against endotoxemia and sepsis in mice and macrophage cultures treated with lipopolysaccharide(LPS). We screened for potential protein targets of Cel using activity-based protein profiling(ABPP). Potential targets were validated using biophysical methods such as cellular thermal shift assays(CETSA) and surface plasmon resonance(SPR). Residues involved in Cel binding to target proteins were identified through point mutagenesis, and the functional effects of such binding were explored through gene knockdown.Results: Cel protected mice from lethal endotoxemia and improved their survival with sepsis, and it significantly decreased the levels of pro-inflammatory cytokines in mice and macrophages treated with LPS(P lity group box 1(HMGB1) protein, reducing the secretion of inflammatory cytokine interleukin(IL)-1β. Cel bound to the Cys residues in lactate dehydrogenase A(LDHA).Conclusions: Cel inhibits inflammation and the Warburg effect in sepsis via targeting PKM2 and HMGB1 protein.

关键词： Celastrol Sepsis Pyruvate kinase M2 High mobility group box 1 Aerobic glycolysis

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Using bovine pituitary extract to increase proliferation of keratocytes and maintain their phenotype in vitro

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International Journal of Ophthalmology(English edition) 2013年第6期6卷 758-765页

作者： Zhong-Zhong Xu zhi-jie li lian-Xin Du Jing li li-Ya Wang Department of Ophthalmology the First Affiliated Hospital of Zhengzhou University Key Laboratory of Corneal Disease Henan Eye Institute Department of Pediatrics Baylor College of Medicine

AIM:To investigate the effects of bovine pituitary extract on the proliferation of keratocytes and maintaining the keratocyte phenotype/n v/tro.METHODS:Single keratocytes were isolated by enzyme digestion for in vitro culture.Three groups were designed according to the different culture media:a bovine pituitary extract(BPE)group,a fetal bovine serum(FBS)group and the control group.The phenotypes and proliferation of cultured cells were evaluated by morphology,immunofluorescent staining and mRNA expression of CD34,Lumican,VSX1,α-SMA and proliferating cell nuclear antigen(PCNA).in the BPE group,cells underwent serial subcultivation,and their phenotypes were identified by immunofluorescent staining.To analyze the proliferation of keratocytes in different concentrations of BPE,six different concentrations were designed to ascertain the most appropriate amount.RESULTS:In the BPE group,the cells spread out and presented dendritic morphology,and their dendrites connected to one another to form networks.On the third passage,most cells maintained their phenotype.In the FBS group,the cells exhibited a dendritic appearance in early cultured stages,but their morphology subsequently changed into a fibroblast-like shape.The number ofdendritic cells in BPE group was more than FBS and control groups.Immunofluorescent staining and realtime polymerase chain reaction(PCR)confirmed that few keratocytes underwent fibroblastic transformation in the BPE and control groups,and that proliferation was higher in the BPE group than in the control group.Although the proliferation was higher in the FBS group,many keratocytes underwent fibroblastic transformation.The analysis of cell morphology and mRNA expressions of CD34,PCNA and VSX1 in six group showed that different concentrations of BPE affected the proliferation obviously but didn’t affect the keratocyte phenotype,and the concentration of 40μg/mL was the most appropriate one.CONCLUSION:BPE can improve the proliferation of keratocytes and maintain their phenotype in vitro.Many keratocyte

关键词： keratocyte bovine pituitary extract proliferation phenotype fibroblastic transformation

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Identification of antimalarial targets of chloroquine by a combined deconvolution strategy of ABPP and MS-CETSA

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Military Medical Research 2023年第1期10卷 64-77页

作者： Peng Gao Yan-Qing liu Wei Xiao Fei Xia Jia-Yun Chen li-Wei Gu Fan Yang liu-Hai Zheng Jun-Zhe Zhang Qian Zhang zhi-jie li Yu-Qing Meng Yong-Ping Zhu Huan Tang Qiao-li Shi Qiu-Yan Guo Ying Zhang Cheng-Chao Xu ling-Yun Dai Ji-Gang Wang Artemisinin Research Center Institute of Chinese Materia MedicaChina Academy of Chinese Medical SciencesBeijing 100700China Department of Traditional Chinese Medicine Southern Medical UniversityGuangzhou 510515China Department of Geriatrics the Second Clinical Medical College of Jinan Universitythe First Affiliated Hospital of Southern University of Science and TechnologyShenzhen People’s HospitalShenzhen 518020GuangdongChina Institute of Molecular and Cell Biology Agency for ScienceTechnology and Research(A*STAR)Singapore 138673Singapore Department of Biological Sciences National University of SingaporeSinga‑pore 117543Singapore

Background: Malaria is a devastating infectious disease that disproportionally threatens hundreds of millions of people in developing countries. In the history of anti-malaria campaign, chloroquine(CQ) has played an indispensable role, however, its mechanism of action(MoA) is not fully understood.Methods: We used the principle of photo-affinity labeling and click chemistry-based functionalization in the design of a CQ probe and developed a combined deconvolution strategy of activity-based protein profiling(ABPP) and mass spectrometry-coupled cellular thermal shift assay(MS-CETSA) that identified the protein targets of CQ in an unbiased manner in this study. The interactions between CQ and these identified potential protein hits were confirmed by biophysical and enzymatic assays.Results: We developed a novel clickable, photo-affinity chloroquine analog probe(CQP) which retains the antimalarial activity in the nanomole range, and identified a total of 40 proteins that specifically interacted and photocrosslinked with CQP which was inhibited in the presence of excess CQ. Using MS-CETSA, we identified 83 candidate interacting proteins out of a total of 3375 measured parasite proteins. At the same time, we identified 8 proteins as the most potential hits which were commonly identified by both methods.Conclusions: We found that CQ could disrupt glycolysis and energy metabolism of malarial parasites through direct binding with some of the key enzymes, a new mechanism that is different from its well-known inhibitory effect of hemozoin formation. This is the first report of identifying CQ antimalarial targets by a parallel usage of labeled(ABPP)and label-free(MS-CETSA) methods.

关键词： Chloroquine Antimalaria Activity-based protein profiling(ABPP) Cellular thermal shift assay(CETSA) Quantitative proteomics

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HDAC-selective Inhibitor Cay10603 Has Single Anti-tumour Effect in Burkitfs Lymphoma Cells by Impeding the Cell Cycle

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Current Medical Science 2019年第2期39卷 228-236页

作者： Xiu-juan MA Gang XU zhi-jie li Fang CHEN Di WU Jia-ning MIAO Yue ZHAN Yang FAN Medical Research Center Shengjing HospitalChina Medical UniversityShenyang 110004China Department of Pediatrics Shengjing HospitalChina Medical UniversityShenyang 110004China Department of Hematology Laboratoiy Shengjing HospitalChina Medical UniversityShenyang 110004China

Histone deacetylases(HDACs)inhibitors are novel in cancer therapy nowadays.HDAC6-selective inhibitors exert advantageous effects due to higher selectivity and less toxicity.We explored the anti-tumor effect and the molecular mechanism of cay 10603,a potent HDAC6 inhibitor in Burkitt's lymphoma cells.Our study revealed cay 10603 inhibited the proliferation of Burkitt's lymphoma cell lines,and induced caspase-dependent apoptosis.Cayl0603 inhibited the expression of CDKs and cyciins to impede cell cycle progression in both Burkitt's lymphoma cell lines.Cay 10603 also showed the additive effect with vpl 6 notably.Our data presented the promising anti-tumor effect of cay 10603 in the Burkitt's lymphoma therapy.

关键词： Burkitt's lymphoma HDAC6 cell cycle apoptosis

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Osteopontin as potential biomarker and therapeutic target in gastric and liver cancers

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World Journal of Gastroenterology 2012年第30期18卷 3923-3930页

作者： Dong-Xing Cao zhi-jie li Xiao-Ou Jiang Yick liang Lum Ester Khin Nikki P Lee Guo-Hao Wu John M Luk Department of Pharmacology and Department of Surgery National University Health SystemNa-tional University of SingaporeSingapore 117597Singapore Department of General Surgery Zhongshan HospitalFudan UniversityShanghai 200032China Department of Oncology Roche R and D Center(China)Ltd.Shanghai 201203China Department of Surgery The University ofHong KongHong KongChina

Gastric cancer and liver cancer are among the most common malignancies and the leading causes of death worldwide,due to late detection and high recurrence rates.Today,these cancers have a heavy socioeconomic burden,for which a full understanding of their pathophysiological features is warranted to search for promising biomarkers and therapeutic targets.Osteopontin (OPN) is overexpressed in most patients with gastric and liver cancers.Over the past decade,emerging evidence has revealed a correlation of OPN level and clinicopathological features and prognosis in gastric and liver cancers,indicating its potential as an independent prognostic indicator in such patients.Functional studies have verified the potential of OPN knockdown as a therapeutic approach in vitro and in vivo .Furthermore,OPN mediates multifaceted roles in the interaction between cancer cells and the tumor microenvironment,in which many details need further exploration.OPN signaling results in various functions,including prevention of apoptosis,modulation of angiogenesis,malfunction of tumor-associated macrophages,degradation of extracellular matrix,activation of phosphoinositide 3-kinase-Akt and nuclear factor-κB pathways,which lead to tumor formation and progression,particularly in gastric and liver cancers.This editorial aims to review recent findings on alteration in OPN expression and its clinicopathological associations with tumor progression,its potential as a therapeutic target,and putative mechanisms in gastric and liver cancers.Better understanding of the implications of OPN in tumorigenesis might facilitate development of therapeutic regimens to benefit patients with these deadly malignancies.

关键词：治疗方案生物标志物骨桥蛋白肝癌胃癌恶性肿瘤靶标病理特点

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linear dipole behavior of single quantum dots encased in metal oxide semiconductor nanoparticles films

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Frontiers of physics 2019年第2期14卷 97-104页

作者： Guo-Feng Zhang Yong-Gang Peng Hai-Qing Xie Bin li zhi-jie li Chang-Gang Yang Wen-li Guo Cheng-Bing Qin Rui-Yun Chen Yan Gao Yu-Jun Zheng lian-Tuan Xiao Suo-Tang Jia State Key Laboratory of Quantum Optics and Quantum Optics Devices Institute of Laser SpectroscopyShanxi UniversityTaiyuan 030006China School of Physics Shandong UniversityJinan 250100China Department of Physics Taiyuan Normal UniversityJinzhong 030619China Collaborative Innovation Center of Extreme Optics Shanxi UniversityTaiyuan 030006China

Understanding of charge/energy exchange processes and interfacial interactions that occur between quantum dots (QDs) and the metal oxides is of critical importance to these QD-based optoelectronic devices. This work reports on linear dipole behavior of single near-infrared emitting CdSeTe/ZnS core/shell QDs which are encased in indium tin oxide (ITO) semiconductor lianoparticles films. A strong polarization anisotropy in photohiminescence emission is observed by defocused wide-field imaging and polarization measuremen11echniques, and the average polarization degree is up to 0.45. A possible mechanism for the observation is presented in which the electrons, locating at single QD surface from ITO by electron transfer due to the equilibration of the Fermi levels, result in a significant Stark distortion of the QD electron/hole wavefunctions. The Stark distortion results in the linear polarization property of the single QDs. The investigation of linear dipole behavior for single QDs encased in ITO films would be helpful for further improving QD-based device performance.

关键词： single quantum dots linear dipole behavior electron transfer polarization property metal oxide nanoparticles

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Cathelicidin a potential therapeutic peptide for gastrointestinal inflammation and cancer

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World Journal of Gastroenterology 2013年第18期19卷 2731-2735页

作者： Jimmy Yip Chuen Chow zhi jie li William Ka Kei Chi Hin Cho Department of Medicine University of California San Diego La Jolla CA 92037 United States Western Australia Institute for Medicine Research the University of Western Australia Institute of Digestive Diseases the Chinese University of Hong Kong Hong Kong China School of Biomedical Sciences Faculty of Medicine the Chinese University of Hong Kong Hong Kong China

Cathelicidins, are host defense peptides synthesized and stored in circulating leukocytes and numerous types of epithelial tissues in particular the gastrointestinal (GI) tract and skin. They have been known for their antimicrobial activities against a variety of microbes. Recently it was discovered that they have other significant biological functions and produce appealing pharmacological actions against inflammation and cancer in the GI tract through defined mechanisms. Experimental evidence shows that these actions could be tissue and disease specific and concentration dependent. This article reviews some of the physiological functions of cathelicidins and also their therapeutic potential in the treatment of inflammation and cancer and also the delivery system for this peptide as targeted therapy for various disorders in the GI tract both in animals and humans.

关键词： Cathelicidin Gastrointestinal tract Cancer Inflammation Ulcer repair

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The clinical and immunological features of pediatric COVID-19 patients in China

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Genes & Diseases 2020年第4期7卷 535-541页

作者： Juan Chen Zhen-Zhen Zhang Yao-Kai Chen Quan-Xin Long Wen-Guang Tian Hai-Jun Deng jie-li Hu Xian-Xiang Zhang Pu-liao Jiang-lin Xiang Dao-Xin Wang Peng Hu Fa-Chun Zhou zhi-jie li Hong-Mei Xu Xue-Fei Cai De-Qiang Wang Yuan Hu Ni Tang Bei-Zhong liu Gui-Cheng Wu Ai-Long Huang The Key Laboratory of Molecular Biology of Infectious Diseases Designated By the Chinese Ministry of Education Department of Infectious DiseasesThe Second Affiliated HospitalChongqing Medical UniversityChongqingChina Department of Infectious Diseases National Clinical Research Center for Child Health and DisordersThe Children’s Hospital of Chongqing Medical UniversityChongqingChina Division of Infectious Diseases Chonqging Public Health Medical CenterChongqingChina Department of Infectious Diseases Yongchuan Hospital Affiliated to Chongqing Medical UniversityChongqingChina Department of Endocrinology Chongqing Three Gorges Central HospitalChongqingChina Department of Clinical Laboratory Chongqing People’s HospitalChongqingChina Department of Infectious Diseases Chongqing Three Gorges Central HospitalChongqingChina Department of Respiration The First Affiliated HospitalChongqing Medical UniversityChongqingChina Department of Emergency The First Affiliated HospitalChongqing Medical UniversityChongqingChina Department of Clinical Laboratory Yongchuan Hospital Affiliated to Chongqing Medical UniversityChongqingChina Department of liver Disease Chongqing Three Gorges Central HospitalChongqingChina

In December 2019,the corona virus disease 2019(COVID-19)caused by novel coronavirus(SARS-CoV-2)emerged in Wuhan,China and rapidly spread worldwide.Few information on clinical features and immunological profile of COVID-19 in paediatrics.The clinical features and treatment outcomes of twelve paediatric patients confirmed as COVID-19 were analyzed.The immunological features of children patients was investigated and compared with twenty adult patients.The median age was 14.5-years(range from 0.64 to 17),and six of the patients were male.The average incubation period was 8 days.Clinically,cough(9/12,75%)and fever(7/12,58.3%)were the most common symptoms.Four patients(33.3%)had diarrhea during the disease.As to the immune profile,children had higher amount of total T cell,CD8t T cell and B cell but lower CRP levels than adults(Plieved in 3e4 days after admitted to hospital.The paediatric patients showed mild symptom but with longer incubation period.Children infected with SARS-CoV-2 had different immune profile with higher T cell amount and low inflammatory factors level,which might ascribed to the mild clinical symptom.We advise that nucleic acid test or examination of serum IgM/IgG antibodies against SARS-CoV-2 should be taken for children with exposure history regardless of clinical symptom.

关键词： Clinical features COVID-19 Immune Paediatrics SARS-CoV-2

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Colorectal cancer screening:The value of early detection and modern challenges

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World Journal of Gastroenterology 2024年第20期30卷 2726-2730页

作者： Jian li zhi-Peng li Wen-jie Ruan Wei Wang Department of Interventional Oncology Municipal Hospital Affiliated to Taizhou UniversityTaizhou 318000Zhejiang ProvinceChina School of Medicine Taizhou UniversityTaizhou 318000Zhejiang ProvinceChina

The screening of colorectal cancer(CRC)is pivotal for both the prevention and treatment of this disease,significantly improving early-stage tumor detection rates.This advancement not only boosts survival rates and quality of life for patients but also reduces the costs associated with treatment.However,the adoption of CRC screening methods faces numerous challenges,including the technical limitations of both noninvasive and invasive methods in terms of sensitivity and specificity.Moreover,socioeconomic factors such as regional disparities,economic conditions,and varying levels of awareness affect screening uptake.The coronavirus disease 2019 pandemic further intensified these challenges,leading to reduced screening participation and increased waiting periods.Additionally,the growing prevalence of early-onset CRC necessitates innovative screening approaches.In response,research into new methodologies,including artificial intelligence-based systems,aims to improve the precision and accessibility of screening.Proactive measures by governments and health organizations to enhance CRC screening efforts are underway,including increased advocacy,improved service delivery,and international cooperation.The role of technological innovation and global health collaboration in advancing CRC screening is undeniable.Technologies such as artificial intelligence and gene sequencing are set to revolutionize CRC screening,making a significant impact on the fight against this disease.Given the rise in early-onset CRC,it is crucial for screening strategies to continually evolve,ensuring their effectiveness and applicability.

关键词： Colorectal cancer Early detection Economic implications Noninvasive tests Quality of life Survival rates Therapeutic modalities Technical challenges

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