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Finite element analysis of multi-piece post-crown restoration using different types of adhesives

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International Journal of Oral Science 2013年第3期5卷 162-166页

作者： Lin-Wei L Guang-Wei Meng zhi-hui liu Department of Engineering Mechanics Jilin University Hospital of Stomatology

The multi-piece post-crown technique is more effective in restoring residual root than other restoration *** types of adhesives have different material properties that affect ***,the choice of adhesive is particularly important for ***,the effect of different kinds of adhesives was not too precise by experimental methods when concerning about individual differences of *** tooth root can only be restored with one type of adhesive in *** the mechanical test,this tooth root cannot be restored with other *** the help of medical imaging technology,reverse engineering and finite element analysis,a molar model can be reconstructed precisely and restored using different types of *** same occlusal and chewing loads were exerted on the same restored residual root models with different types of adhesives *** of von Mises stress analysis showed that the adhesives with low Young’s modulus can protect the root canal ***,a root canal concentration is apparently produced around the root canal orifice when *** with large Young’s modulus can buffer the stress concentration of the root canal ***,the root canal tissue may be destroyed because the adhesive is too hard to buffer the load.

关键词： adhesive finite element analysis molar restoration multi-piece post-crown stomatology

Finkelstein Reaction in Functionalized Crown-ether Ionic Liquids

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Chinese Chemical Letters 2006年第12期17卷 1531-1533页

作者： Xiao Hua WANG Han zhi WANG hui liu Yuan KOU PKU Green Chemistry Center Beijing National Laboratory for Molecular Sciences College of Chemistry and Molecular Engineering Peking University Beijing 100871

Functional crown-ether ionic liquids were used as catalytic green solvents of Finkelstein reaction of 1-bromooctane and iodide. The rate and yield of the reaction were obvious improved compared with that using crown e... 详细信息

关键词： Functionalized ionic liquids green chemistry crown ether nucleophilic displacement,Finkelstein reaction.

Relationship between NRAGE and the radioresistance of esophageal carcinoma cellline TE13R120

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Chinese Journal of Cancer 2010年第10期29卷 900-906页

作者： Xiao-Ying Xue zhi-He liu Feng-Min Jing Yan-Ge Li hui-zhi liu Xian-Shu Gao Department of Radiation Oncology Second Hospital of Hebei Medical University Shijiazhuang Hebei 050000 P. R. China Department of Radiation Oncology Peking Unversity First Hospital Beijing 100034 P. R. China

Background and Objective: The mRNA levels of 59 genes, detected by cDNA microarray, were up-regulated in the radioresistant human esophageal cacinoma cell line TE13R120 as compared with its parental cell line TE13 before and after radiation, and the expression of NRAGE gene showed a gradually up-regulating tendency. This study aimed to further detect the differences of NRAGE gene and protein expression and apoptosis between TE13R120 and TE13 cells, and to investigate the relationship between the NRAGE and the radioresistance of TE13R120 cells and its mechanism. Methods: The two cell lines were irradiated by 60 Co γ-ray at different conditions. Reverse transcription- polymerase chain reaction (RT-PCR), Western blot, and immunocytochemistry were used to detect the expression of NRAGE. Flow cytometry (FCM) was used to detect the cell apoptosis before and after irradiation. Results: The mRNA level of NRAGE was higher in TE13R120 cells than in TE13 cells before and after irradiation (before radiation: 0.25 ± 0.03 vs. 0.49 ± 0.03; 4 Gy 4 h: 0.31 ± 0.03 vs. 0.53 ± 0.02; 4 Gy 16 h: 0.32 ± 0.04 vs. 0.59 ± 0.04; 4 Gy 24 h: 0.36 ± 0.05 vs. 0.72 ± 0.04; 2 Gy 12 h: 0.32 ± 0.02 vs. 0.64 ± 0.04; 6 Gy 12 h: 0.36 ± 0.02 vs. 0.79 ± 0.05; 10 Gy 12 h: 0.46 ± 0.04 vs. 0.85 ± 0.01; P < 0.01), and the mRNA level of NRAGE was increased gradually with the increase of radiation dose and time in the two cell lines (P < 0.05 and P < 0.01). Western blot results showed no difference of NRAGE protein level in cytoplasm between TE13R120 cells and TE13 cells before and after irradiation, but its level in nuclei was higher in TE13R120 cells than in TE13 cells at different radiation time and dosages. Immunocytochemistry showed similar results as Western blot. FCM showed no significant difference in apoptosis rate between TE13R120 and TE13 cells before and after radiation. Conclusion: NRAGE may play an important role in the radiation responses of the two cell lines, and may participate in the formatio

关键词：细胞系抗辐射食管癌逆转录聚合酶链反应 mRNA水平免疫细胞化学 GE基因芯片检测

ERCC1 polymorphism, expression and clinical outcome of oxaliplatin-based adjuvant chemotherapy in gastric cancer

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World Journal of Gastroenterology 2008年第41期14卷 6401-6407页

作者： Zhao-hui Huang Dong Hua Xiang Du Li-Hua Li Yong Mao zhi-hui liu Ming-Xu Song Xi-Ke Zhou Institute the Fourth Affiliated Hospital of Suzhou University Wuxi 214062 Jiangsu Province China Department of Pathology Cancer Hospital Fudan University Shanghai 200032 China

AIM： TO determine the influence of excision repair cross complementing group 1 （ERCC1） codon 118 polymorphism and mRNA level on the clinical outcome of gastric cancer patients treated with oxaliplatin-based adjuvant chemotherapy. METHODS： Eighty-nine gastric cancer patients treated with oxalipatin-based adjuvant chemotherapy were included in this study. ERCC1 codon 118 C/T polymorphism was tested by polymerase chain reaction-ligation detection reaction （PCR-LDR） method in peripheral blood lymphocytes of those patients; and the intratumoral ERCC1 mRNA expression was measured using reverse transcription PCR in 62 patients whose tumor tissue specimens were available. RESULTS： No significant relationship was found between ERCC1 codon 118 polymorphism and ERCC1 mRNA level. The median relapse-free and overall survival period was 20.1 mo and 28.4 too, respectively. The relapse-free and overall survivals in patients with lOW levels of ERCC1 mRNA were significantly longer than those in patients with high levels （P 〈 0.05）, while there was no significant association found between ERCC1 118 genotypes and the disease prognosis. Multivariate analysis also showed that ERCC1 mRNA level was a potential predictor for relapse and survival in gastric cancer patients treated with oxaliplatin-based adjuvant chemotherapy （P 〈 0.05）. CONCLUSION： ERCC1 codon 118 polymorphisrn has no significant impact on ERCC1 rnRNA expression, and the intraturnoral ERCC1 rnRNA level but not codon 118 polymorphisrn may be a useful predictive parameter for the relapse and survival of gastric cancer patients receiving oxaliplatin-based adjuvant chemotherapy.

关键词： Gastric cancer Adjuvant chemotherapy Excision repair cross complementing group 1 Gene polymorphism

Two new isospirostanol sapogenins from Reineckia carnea

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Chinese Chemical Letters 2012年第11期23卷 1240-1242页

作者： Yao Wang Na Han Ling Li Chen zhi hui liu Li Bo Zou Jun Yin School of Traditional Chinese Materia Medica Shenyang Pharmaceutical UniversityShenyang 110016China Development and Utilization Key Laboratory of Northeast Authentic Materials Research and Development in Liaoning Province School of Traditional Chinese Materia MedicaShenyang Pharmaceutical UniversityShenyang 110016China

Two new isospirostanol sapogenins named （25R）-5/%spirostane-（1αα 3a）-diol （1） and （25R）-5/%spirostane-（1α, 2α, 3α, 4α）- tetrol （2） were isolated from the whole herb ofReineckia carnea （Andr.） Kunth... 详细信息

关键词： Reineckia carnea (Andr) Kunth. Chemical constituent Isospirostanol sapogenin

Identification of a Positive Cis-Element Upstream of Human NKX3.1 Gene

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Acta Biochimica et Biophysica Sinica 2005年第11期37卷 773-778页

作者： An-Li JIANG Peng-Ju ZHANG Xiao-Yan HU Wei-Wen CHEN Feng KONG zhi-Fang liu hui-Qing YUAN Jian-Ye ZHANG Department of Biochemistry,Medical School of Shandong University,Jinan 250012,China Department of Biochemistry Medical School of Shandong UniversityJinan 250012 China

NKX3.1 is a prostate-specific homeobox gene related to prostate development and *** this work,we aimed to identify precisely the functional cis-element in the 197 bp region(from-1032 to -836 bp)of the NKX3.1 promoter(from -1032 to +8bp),which was previously identified topresent positive regulatory activity on NKX3.1 expression,by deletion mutagenesis analysis and electro-phoretic mobility shift assay(EMSA).A 16 bp positive cis-element located between -920 and -905 bpupstream of the NKX3.1 gene was identified by deletion mutation analysis and proved to be a functionalpositive cis-element by *** will be important to further study the functions and regulatory mechanismsof this positive cis-element in NKX3.1 gene expression.

关键词： human NKX3.1 cis-acting element positive regulation mutagenesis

Up-regulation of NKX3.1 Expression and Inhibition of LNCaP Cell Proliferation Induced by an Inhibitory Element Decoy

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Acta Biochimica et Biophysica Sinica 2005年第5期37卷 335-340页

作者： An-Li JIANG Xiao-Yan HU Peng-Ju ZHANG Mei-Lan HE Feng KONG zhi-Fang liu hui-Qing YUAN Jian-Ye ZHANG Department of Biochemistry, Medical School of Shandong University,Jinan 250012, China Department of Biochemistry Medical School of Shandong UniversityJinan 250012 China

NKX3.1 is an androgen-regulated prostate-specific homeobox gene that is thought to play animportant role in prostate development and ***3.1 acts as a tumor suppressor gene specificallyin the ***-regulation of NKX3.1 gene offers a promising gene therapy for prostate *** strategy has been developed and is considered a useful tool for regulating gene expression and *** our previous studies,we identified a 20 bp inhibitory element upstream of the NKX3.1 *** this study,we focused on using the 20 bp inhibitory element decoy to block negative regulation of theNKX3.1 gene and to up-regulate NKX3.1 expression using synthetic double-stranded oligodeoxynucleotidesof the 20 bp inhibitory *** found in an electrophoretic mobility shift assay experiment that the 20 bpinhibitory decoy presented competitive binding to a specific binding protein of the 20 bp inhibitory element inprostate cancer cell line *** luciferase reporter gene assays,we found that the 20 bp inhibitory decoycould enhance NKX3.1 promoter activity,and RT-PCR and Western blot analysis revealed that NKX3.1expression was up-regulated effectively by the transfection with the 20 bp inhibitory ***,cell proliferation was inhibited by up-regulated NKX3.1 expression induced by the 20 bp inhibitory decoy.

关键词： human NKX3.1 gene cis-acting element negative regulation decoy oligodeoxynucleotide cell proliferation

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不同隔板构型下带反应超声速混合层实验

航空动力学报 2017年第1期32卷 27-34页

作者：李朗晏至辉刘彧中国科学技术大学工程科学学院近代力学系合肥230027 中国空气动力研究与发展中心吸气式高超声速技术研究中心四川绵阳621000 国防科学技术大学航天与材料工程学院长沙410073

为了考察超声速混合层混合、燃烧流场特性,并探索其被动增强方法,利用高速纹影、自发辐射成像和平面激光诱导荧光(PLIF)技术对压力匹配条件下的超声速混合层在不同隔板构型下的流场特性进行研究.结果表明:相对于对混合层混合的影响,凹... 详细信息

为了考察超声速混合层混合、燃烧流场特性,并探索其被动增强方法,利用高速纹影、自发辐射成像和平面激光诱导荧光(PLIF)技术对压力匹配条件下的超声速混合层在不同隔板构型下的流场特性进行研究.结果表明:相对于对混合层混合的影响,凹腔对燃烧的影响明显受到抑制;尾部交错结构隔板促进混合层流向涡的产生,大大提高了混合层内燃烧强度.

关键词：超声速混合层自发辐射 OH基PLIF 燃烧流向涡

Effects of leptin-modified human placenta-derived mesenchymal stem cells on angiogenic potential and peripheral inflammation of human umbilical vein endothelial cells(HUVECs) after X-ray radiation

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2020年第4期21卷 327-340页

作者： Shu CHEN Qian WANG Bing HAN Jia WU Ding-kun liu Jun-dong ZOU Mi WANG zhi-hui liu Department of Prosthodontics Hospital of StomatologyJilin UniversityChangchun 130021China Department of Thoracic Surgery the Second Hospital of Jilin UniversityChangchun 130041China Department of Radiology the Second Hospital of Jilin UniversityChangchun 130041China

Combined radiation-wound injury(CRWI) is characterized by blood vessel damage and pro-inflammatory cytokine deficiency. Studies have identified that the direct application of leptin plays a significant role in angiogenesis and inflammation. We established a sustained and stable leptin expression system to study the mechanism. A lentivirus method was employed to explore the angiogenic potential and peripheral inflammation of irradiated human umbilical vein endothelial cells(HUVECs). Leptin was transfected into human placenta-derived mesenchymal stem cells(HPMSCs) with lentiviral vectors. HUVECs were irradiated by X-ray at a single dose of 20 Gy. Transwell migration assay was performed to assess the migration of irradiated HUVECs. Based on the Transwell systems, co-culture systems of HPMSCs and irradiated HUVECs were established. Cell proliferation was measured by cell counting kit-8(CCK-8) assay. The secretion of pro-inflammatory cytokines(human granulocyte macrophage-colony stimulating factor(GM-CSF), interleukin(IL)-1α, IL-6, and IL-8) was detected by enzyme-linked immunosorbent assay(ELISA). The expression of pro-angiogenic factors(vascular endothelial growth factor(VEGF) and basic fibroblast growth factor(b FGF)) mRNA was detected by real-time quantitative polymerase chain reaction(RT-qPCR) assay. Relevant molecules of the nuclear factor-κB(NF-κB) and Janus kinase(JAK)/signal transducer and activator of transcription(STAT) signaling pathways were detected by western blot assay. Results showed that leptin-modified HPMSCs(HPMSCs/leptin) exhibited better cell proliferation, migration, and angiogenic potential(expressed more VEGF and bFGF). In both the single HPMSCs/leptin and the co-culture systems of HPMSCs/leptin and irradiated HUVECs, the increased secretion of pro-inflammatory cytokines(human GM-CSF, IL-1α, and IL-6) was associated with the interaction of the NF-κB and JAK/STAT signaling pathways. We conclude that HPMSCs/leptin could promote angiogenic potential

关键词： Leptin Angiogenesis Pro-inflammatory cytokines X-ray radiation Human placenta-derived mesenchymal stem cells(HPMSCs) Human umbilical vein endothelial cells(HUVECs)