Relationship between NRAGE and the radioresistance of esophageal carcinoma cellline TE13R120

作     者：Xiao-Ying Xue Zhi-He Liu Feng-Min Jing Yan-Ge Li Hui-Zhi Liu Xian-Shu Gao 

作者机构：Department of Radiation Oncology Second Hospital of Hebei Medical University Shijiazhuang Hebei 050000 P. R. China Department of Radiation Oncology Peking Unversity First Hospital Beijing 100034 P. R. China 

出 版 物：《Chinese Journal of Cancer》 (Chin. J. Cancer)

年 卷 期：2010年第29卷第10期

页      面：900-906页

核心收录：

学科分类：08[工学] 09[农学] 0901[农学-作物学] 0836[工学-生物工程] 0825[工学-航空宇航科学与技术] 090102[农学-作物遗传育种] 

基　　金：National Natural Science Foundation of China (No.C03031802) Natural Science Foundation of Hebei Province(No.C2009001151) 

主　　题：细胞系 抗辐射 食管癌 逆转录聚合酶链反应 mRNA水平 免疫细胞化学 GE基因 芯片检测 

摘      要：Background and Objective: The mRNA levels of 59 genes, detected by cDNA microarray, were up-regulated in the radioresistant human esophageal cacinoma cell line TE13R120 as compared with its parental cell line TE13 before and after radiation, and the expression of NRAGE gene showed a gradually up-regulating tendency. This study aimed to further detect the differences of NRAGE gene and protein expression and apoptosis between TE13R120 and TE13 cells, and to investigate the relationship between the NRAGE and the radioresistance of TE13R120 cells and its mechanism. Methods: The two cell lines were irradiated by 60 Co γ-ray at different conditions. Reverse transcription- polymerase chain reaction (RT-PCR), Western blot, and immunocytochemistry were used to detect the expression of NRAGE. Flow cytometry (FCM) was used to detect the cell apoptosis before and after irradiation. Results: The mRNA level of NRAGE was higher in TE13R120 cells than in TE13 cells before and after irradiation (before radiation: 0.25 ± 0.03 vs. 0.49 ± 0.03; 4 Gy 4 h: 0.31 ± 0.03 vs. 0.53 ± 0.02; 4 Gy 16 h: 0.32 ± 0.04 vs. 0.59 ± 0.04; 4 Gy 24 h: 0.36 ± 0.05 vs. 0.72 ± 0.04; 2 Gy 12 h: 0.32 ± 0.02 vs. 0.64 ± 0.04; 6 Gy 12 h: 0.36 ± 0.02 vs. 0.79 ± 0.05; 10 Gy 12 h: 0.46 ± 0.04 vs. 0.85 ± 0.01; P 0.01), and the mRNA level of NRAGE was increased gradually with the increase of radiation dose and time in the two cell lines (P 0.05 and P 0.01). Western blot results showed no difference of NRAGE protein level in cytoplasm between TE13R120 cells and TE13 cells before and after irradiation, but its level in nuclei was higher in TE13R120 cells than in TE13 cells at different radiation time and dosages. Immunocytochemistry showed similar results as Western blot. FCM showed no significant difference in apoptosis rate between TE13R120 and TE13 cells before and after radiation. Conclusion: NRAGE may play an important role in the radiation responses of the two cell lines, and may participate in the formatio