Identification of a Positive Cis-Element Upstream of Human NKX3.1 Gene

作     者：An-Li JIANG Peng-Ju ZHANG Xiao-Yan HU Wei-Wen CHEN Feng KONG Zhi-Fang LIU Hui-Qing YUAN Jian-Ye ZHANG Department of Biochemistry,Medical School of Shandong University,Jinan 250012,China An-Li JIANG Peng-Ju ZHANG Xiao-Yan HU Wei-Wen CHEN Feng KONG Zhi-Fang LIU Hui-Qing YUAN Jian-Ye ZHANG Department of Biochemistry,Medical School of Shandong University,Jinan 250012,China

作者机构：Department of Biochemistry Medical School of Shandong UniversityJinan 250012 China 

出 版 物：《Acta Biochimica et Biophysica Sinica》 (生物化学与生物物理学报(英文版))

年 卷 期：2005年第37卷第11期

页      面：773-778页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071007[理学-遗传学] 

基　　金：This work was supported by the grants from the National Natural Science Foundation of China(No.30470952) the Natural Science Foundation of Shandong Province(No.Y2004C26) 

主　　题：human NKX3.1 cis-acting element positive regulation mutagenesis 

摘      要：NKX3.1 is a prostate-specific homeobox gene related to prostate development and *** this work,we aimed to identify precisely the functional cis-element in the 197 bp region(from-1032 to -836 bp)of the NKX3.1 promoter(from -1032 to +8bp),which was previously identified topresent positive regulatory activity on NKX3.1 expression,by deletion mutagenesis analysis and electro-phoretic mobility shift assay(EMSA).A 16 bp positive cis-element located between -920 and -905 bpupstream of the NKX3.1 gene was identified by deletion mutation analysis and proved to be a functionalpositive cis-element by *** will be important to further study the functions and regulatory mechanismsof this positive cis-element in NKX3.1 gene expression.