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The prolonged effect of glucagon-like peptide 2 pretreatment on growth performance and intestinal development of weaned piglets

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Journal of Animal Science and Biotechnology 2016年第4期7卷 579-587页

作者： Qiuhong Deng Gang Jia Hua Zhao zheng li chen Xiao ling chen Guang mang liu Kang ning Wang Animal Nutrition Institute Sichuan Agricultural University College of Animal Medicine Sichuan Agricultural University

Background： Glucagon-like peptide 2 （GLP-2） is a potent epithelium-specific intestinal growth factor. The aim of this study was to demonstrate the prolonged effect of GLP-2 on the growth performance of weaned piglets. Forty piglets weaned at the age of 28 d with an average BW of 6.8 ＋ 0.4 kg were assigned to four treatments：（i） non- challenged control; （ii） LPS-challenged control; （iii） LPS ＋ low GLP-2; and （iv） LPS ＋ high GLP-2. Piglets in groups （i）, （ii）, and （iv） were s.c. injected with PBS supplemented with human [Gly2]GLP-21-34 at doses of 0, 2 and 10 nmol/kg BW per day for seven consecutive days. BW, gain：feed ratio （G：F）, and plasma GLP-2 levels were determined on d 0 7, and 14 after weaning. Piglets were challenged with i.p. administration of Escherichia coil lipopolysaccharide （LPS） at a dose of 100 pg/kg on d 14 to induce intestinal damage. Twenty-four hours later, intestinal tract samples were collected to assess intestinal morphology and quantify enzyme activity. Results： Plasma GLP-2 levels decreased after weaning, but in the high GLP-2 group, plasma GLP-2 was maintained on d 7 and even increased to a level higher than the preweaning level on d ]4 （P 〈 0.05）. High GLP-2 treatment significantly increased the duodenal, jejunal and ileal weight, as well as the gross weight of the small intestine （SI）, and the SI weight index （P 〈 0.05）. LPS caused villous atrophy and disrupted intestinal morphology in the duodenum, jejunum and ileum. GLP-2 also significantly increased the villus height and the villus height/crypt depth ratio （VCR） of the duodenum, jejunum, and ileum （P 〈 0.05）. Histological examination revealed that in GLP-2-treated groups, the integrity of the villus was maintained, and the villus was protected against LPS-induced damage. GLP-2 significantly increased the activity of alkaline phosphatase （AKP）, y-glutamyltranspeptidase （y-G-i-）, and pancreatic lipase in the duodenum and jejunum （P 〈 0.05）. GLP-2 treatment also significantly increased th

关键词： Escherichia coil lipopolysaccharide Glucagon-like peptide-2 Growth performance Intestinal enzymes Smallintestinal morphology Weaned piglets

The Effect of Glycyl-Glutamine Dipeptide Concentration on Enzyme Activity, Cell Proliferation and Apoptosis of Jejunal Tissues from Weaned Piglets

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Agricultural Sciences in China 2011年第7期10卷 1088-1095页

作者： Wang Hui Jia Gang chen zheng-li Huang Lan Wu Cai-mei Wang Kang-ning Institute of Animal Nutrition Sichuan Agricultural University Ya＇an 625014 P.R.China College of Animal Medicine Sichuan Agriculture University Ya＇an 625014 P.R.China

An experiment was conducted in a singly factorial design to study the effect of glycyl-glutamine dipeptide on enzyme activity, cell proliferation and apoptosis of jejunal tissues from weaned piglets at different glycyl-glutamine concentration levels of 2, 4, 10, 20, and 30 mmol L-1, respectively. The glutaminase activity, diamine oxidase （DAO） activity, cell peoliferation, apoptosis, and perotein metabolism were measured by the tissue culture method in vitro using jejunal tissues. The immunohistochemical method was used to study the cell proliferation and apoptosis of jejunal tissues. The results showed that compared to the blank control, the percentage and MOD value of BrdU-positicve cells incubated with glycyl-glutamine dipeptide solution were significantly （P0.05） increased. Accordingly, the percentage and MOD value of caspase-3-positive cells from tissue incubated with glycyl-glutamine dipeptide were notably lower （P0.05） than that from the control treatment. The glycyl-glutamine dipeptide increased the glutaminase activity, DAO activity and protein content of jejunal tissues, as the dipeptide concentration was on the rise （P0.05）. These results indicated that glycyl-glutamine dipeptide affected the jejunum development and adaptation of weaned piglets, and the function might be fulfilled by enhancing the glutamine-related enzyme activity, thereby increasing the consumption of glutamine, and then improving the jejunal cell proliferation and suppressing cell apoptosis. The effects of glycyl-glutamine dipeptide relied in a dose-dependent manner, and the maximum effect was achieved at 20-30 mmol L-1 glycyl-glutamine dipeptide.

关键词： glycyl-glutamine dipeptide cell proliferation apoptosis diamine oxidase glutaminase

Diffusion MRI studies of experimental animal models and patients with stroke

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Chinese Medical Journal 1999年第2期 81-86页

作者： zheng Guang chen, Tieqiang li and Tomas Hindmarsh

Abstract Purpose This overview summarises the diffusion magnetic resonance imaging (MRI) results both from the experimental animal model studies and clinical investigations in acute cerebral ischemic *** Sources An English literature searched using MEDliNE (1990-1997).Study Selection Over 80 papers and reviews published in the last eight years and our partial results were *** and Conclusions Diffusion weighted magnetic resonance imaging (DWI) has been extensively used to study acute cerebral ischemia both in experimental animal models and in patients. DWI yields signal intensity contrast modulated by water molecular self diffusion. By recording a series of DWI with different amplitudes of the displacement encoding gradient, the apparent diffusion coefficient (ADC) can be quantitatively mapped. DWI combined with quantitative ADC mapping can provide earlier and more precise evaluation of the ischemic zones. However, the biophysical mechanisms behind the decreased ADC in acute cerebral ischemia are still not fully understood.

关键词： MRI studies experimental animal models Diffusion and

Effects of Estrogen on ER, NGF, and ChAT Expression in Cerebellum of Aging Female Sprague-Dawley Rat

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Agricultural Sciences in China 2007年第3期6卷 368-374页

作者： chen zheng-li FAN Guang-li LUO Qi-hui ZHU Chun-mei HUANG Yi-dan College of Animal Science and Technology Northwest A＆F University Yangling 712100 P.R.China College of Animal Science and Technology Sichuan Agricultural University Yaan 625014 P.R.China

This article discusses the effects of estrogen on the expression of estrogen receptor （ER）, nerve growth factor （NGF）, and choline acetyltransferase （CHAT） in the cerebellum of rats. The model of aging female rat was established to study the expression and distribution of ER, NGF, and ChAT in the cerebellum following 17β-estradiol treatment using the technique of immunohistochemical ultrasensitive SP in sprague-dawley rat. The immunoreactive productions were distributed in stratum Purkinje cell, nucleus dentatus, nucleus interpositus, and nucleus fastigii of cerebellum, and the ER positive production was mainly located in the plasma, cytoplasmic membrane, and neurite, and also existed in nucleus. The general tendency of the expression of ER, NGF, and ChAT positive production in the cerebellum cortex and nuclei of aging rat significantly decreases, while the intensity and quantity of the immunoreactive production ascends predominantly after 17β-estradiol treatment. Simultaneously, the positive neurite of Purkinje cell shows a similar tendency. The above- mentioned results suggest that the estrogen upregulates the expression of NGF and CHAT, and plays a vital role in sustaining and protecting the structure and function of cerebellum neurons. Furthermore, the similarity of their changing tendency implies that they were correlated and cooperated during the course in effect of estrogen on cerebellum. It also showed that the action of estrogen in cerebellum could be via genomic and nongenomic mechanism.

关键词： estrogen receptor (ER) nerve growth factor (NGF) choline acetyltransferase (CHAT) immunohistochemical ultrasensitive SP cerebellum rat

Intron-Targeted Intron-Exon Splice Conjunction (IT-ISJ) Marker and Its Application in Construction of Upland Cotton linkage Map

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Agricultural Sciences in China 2008年第10期7卷 1172-1180页

作者： zheng Jing ZHANG zheng-sheng chen li WAN Qun HU Mei-chun WANG Wei ZHANG Ke liU Da-jun chen Xiao WEI Xin-qi Key Laboratory of Biotechnology ＆ Crop Quality Improvement Ministry of Agriculture/Southwest University Chongqing 400716 P.R.China

To develop a new DNA maker, which could be used in genetic diversity analysis and genetic map construction in plants, IT-ISJ （intron targeted intron-exon splice junction） primer combinations, which were designed according to the intronexon splice junction conserved sequences, were used to construct cotton genetic linkage map in the present study. 49 out of 704 IT-ISJ primer combinations showed polymorphism between upland cotton high quality cultivar Yumian 1 and multiple dominant gene line T586, and the polymorphic primer combinations accounted for 7.0% of total primer combinations. 49 IT-ISJ primer combinations were used to genotype 270 F2：7 recombinant inbred lines developed from （Yumian 1 × T586） F2, and 58 IT-ISJ loci were obtained. 58 IT-ISJ, together with 150 SSR and 8 morphological loci, were used to conduct linkage analysis, and a linkage map including 22 linkage groups and 113 loci （49 IT-ISJ, 62 SSR, and 2 morphological loci） was constructed. The linkage map covered 714.5 cM with an average interval of 6.3 cM between two markers, accounting for 16.1% of cotton genome. The present study demonstrated that the polymorphism of IT-ISJ marker is high, and it could be effectively applied in plant genetic map construction.

关键词： IT-ISJ (intron targeted intron-exon splice junction) linkage map upland cotton (Gossypium hirsutum L.)

Evolution and stability of linux kernels based on complex networks

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Science China(Information Sciences) 2012年第9期55卷 1972-1982页

作者： WANG Lei,WANG zheng,YANG chen & ZHANG li School of Computer Science and Engineering,Beihang University,Beijing 100191,China 1. School of Computer Science and Engineering Beihang University Beijing 100191 China

This paper presents a novel method to study linux kernel evolution using complex *** investigated the node degree distribution and average path length of the call graphs corresponding to the kernel modules of 223 different versions(V1.1.0 to V2.4.35),we found that the call graphs are scale-free and smallworld *** on the relationship between average path length and nodes,we propose a method to find unusual points during linux kernel evolution using the slope of the average path *** the unusual points we identify major structural changes in kernel modules.A stability coefficient is also proposed to describe quantitatively the stability of kernel modules during ***,we verify our result through Vasa’s metrics method.

关键词： complex networks operating system scale-free networks small-world networks average path length

Insulin/PI3K/AKt Signaling Pathway Mediates Cardiac Hypertrophy in FKBP12.6 Deficient Male Mice

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生物物理学报 2009年第S1期25卷 206-207页

作者： zheng chen, zheng-zheng li, Wen-Xuan Yin, Guangju Ji Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101

Male mice deficient in FKBP12.6 display cardiac hypertrophy, but the underlying mechanism is still unclear. Here we report that the cardiac hypertrophy is a physiological response

关键词： AKT, insulin cardiac hypertrophy male FKBP12.6 null mice

Microscopic phase-field simulation including elastic strain energy for early precipitation process of Ni-Al alloys with medium Al content

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Progress in Natural Science:Materials International 2005年第12期15卷 1104-1109页

作者： LU Yanli,chen zheng,li Yongsheng,WANG Yongxin and CHU Zhong State Key Laboratory of Solidification Processing,School of Materials Science and Engineering,Northwestern Polytechnical University,Xi’an 710072,China State Key Laboratory of Solidification Processing School of Materials Science and Engineering Northwestern Polytechnical University Xi''an 710072 China

The early precipitation process of Ni-Al alloy was studied on the atomic scale based on the microscopic phase-field kinetic *** investigated the effect of elastic strain energy on precipitation mechanism and morphological evolution of the *** results show that at the early stage of precipitation,γ’ ordered phase presents non-directional and irregular shape during the process of aging,the γ’ ordered phases change into the quadrate shape and their orientations become more obvious; at the later stage,the γ’ precipitates present quadrate shape with round corner and align along the [100] and [010] *** mechanism of early precipitation for Ni-13at.%A1 alloy is the mixed mechanism of non-classical nucleation growth and spinodal decomposition and near to non-classical nucle-ation growth,and the mechanism of early precipitation for Ni-15.8at.% Al alloy is the mixed mechanism of non-classical nucleation growth and spinodal decomposition and near to spinodal decomposition.

关键词： Ni-Al alloy early precipitation microscopic phase-field simulation elastic strain energy

Effect of orthodontic force on inflammatory periodontal tissue remodeling and expression of IL-6 and IL-8 in rats

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Asian Pacific Journal of Tropical Medicine 2013年第10期6卷 757-761页

作者： Jian-Hao Yang zheng-chen li Wei-Dong Kong Wu Zhang Ying-Ping Jia Yue-Lan Zhang lin-Bo liu Xue-Ping Han Department of Stomatology First Affiliated Hospital of Zhengzhou University Stomatological Medical College of zhengzhou University Zhongyuan RoadZhengzhou City Department of Anesthesia First Affiliated Hospital of Zhengzhou University Children's Hospital of zhengzhou Zhengzhou City Department of Stomatology First Affiliated Hospital of Jinan University Children's Hospital of zhengzhou Stomatological Medical College of zhengzhou University Department of Plastic and Cosmetology First Affiliated Hospital of Zhengzhou University

Objective:To investigate effect of orthodontic force on inflammatory periodontal tissue remodeling and expression of IL-6 and IL-8 in ***:Eighty SD rats were randomly divided into 4 groups,blank control group(group A)with 5 rats,treatment normal group(group B)with 25 rats,inflammation control group(group(group C)with 25 rats,inflammation treatment group(group D)with 25 *** and histomorphometric analysis was performed to measure the expression of IL-6,IL-8 and the firet molar to the recent movement in the ***:The expression of IL-8 reached a maximum on day 5 and declined thereafter in group B;the expression of IL-6 reached a maximum on day 5 in group *** expression of IL-6 and IL-8 was gradually weakened with time in group *** expression of IL-6 and IL-8 were high,and reached a maximum on day 5 and declined thereafter in group *** of positive cells in group D were higher than group B at each time point(P<0.05).The time which 0.49 N orthodontic force was loaded was longer,orthodontic tooth movement distance was *** distance in group D were longer than group B(P<0.05).Conclusions:Orthodontic force as well as inflammatory stimulus can evoke the expression of IL-6 and *** the combined effects of inflammation and orthodontic force,the expression of IL-6,IL-8will increase.

关键词： Orthodontic tooth movement Inflammatory periodontal tissue Interleukin-6 Interleukin-8