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Expression patterns and functional divergence of homologous genes accompanied by.poly.loidization in cotton(Gossy.ium hirsutum L.)

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Science China(Life Sciences) 2020年第10期63卷 1565-1579页

作者： zhen Peng Hua Cheng Gaofei Sun Zhaoe Pan Xiao Wang Xiaoli geng Shoupu He Xiongming Du Research Base Anyang Institute of TechnologyState Key Laboratory of Cotton Biology/Institute of Cotton ResearchChinese Academy of Agricultural Science(ICRCAAS)Anyang 455000China zhengzhou Research Base State Key Laboratory of Cotton BiologyZhengzhou UniversityZhengzhou 450001China Any.ng Institute of Technology Anyang 455000China

Naturally.allotetraploid cotton has been widely.used as an ideal model to investigate gene expression remodeling as a consequence of ***,the global gene pattern variation during early.fiber development was *** this study.through RNA-seq technology.we comprehensively.investigated the expression patterns of homologous genes between allotetraploid cotton(***)and its diploid progenitors(*** and ***)at the fiber early.development *** tetraploid cotton,genes showed expression level dominance(ELD)bias toward the A *** phenomenon was explained by.the up-/downregulation of the homologs from the nondominant progenitor(D genome).Gene ontology.GO)enrichment results indicated that the ELD-A genes might be a prominent cause responsible for fiber property.change through regulating the fatty.acid biosy.thesis/metabolism and microtubule procession,and the ELD-D genes might be involved in transcription regulation and stress *** addition,the number and proportion of completely.A-and D-subfunctionalized gene were similar at different fiber development ***,for neofunctionalization,the number and proportion of reactivated D-derived genes were greater than those of A at 3 and 5 ***,we found that some homologous genes belonging to several specific pathway. might create novel asy.metric transcripts between two subgenomes during poly.loidization and domestication process,further making the fiber property.meet the human *** study.identified determinate pathway. and their involved genes between allotetraploid cotton and their progenitors at early.fiber development stages,providing new insights into the mechanism of cotton fiber evolution.

关键词： Gossy.ium fiber development poly.loid evolution expression level dominance sub-and neofunctionalization

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Total Sy.thesis of N-Methy.sansalvamide A

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Chinese Chemical Letters 2006年第6期17卷 761-764页

作者： Shou Xin LIU y.n Lou geng Xia TIAN Xiao Li zhen Jian Rong HAN College of chemical ＆ pharmaceutical Engineering Hebei University of Science ＆ Tecnnology Shijiazhuang 050018

Total sy.thesis of N-methy.sansalvamide A was accomplished in solution phase by.a convergent approach. An N-Boc-td-depsipepide 6 and a dipeptide ester 10 were prepared in the y.eld of 89% and 91%, respectively. Cy.liz... 详细信息

关键词： Sy.thesis N-methy.sansalvamide A solution phase cy.lic depsipeptide.

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Effect of high temperature on the expressions of genes encoding starch sy.thesis enzy.es in developing rice endosperms

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Journal of Integrative Agriculture 2015年第4期14卷 642-659页

作者： CAO zhen-zhen PAN Gang WANG Fu-biao WEI Ke-su LI Zhao-wei SHI Chun-hai geng Wei CHENG Fang-min College of Agriculture and Biotechnology Zhejiang University

High temperature is the major environmental factor affecting grain starch properties of cooking rice cultivars. In this study. two non-waxy.indica rice genoty.es, cv. 9311 and its mutant with extremely.high amy.ose phenoty.e（9311eha） were used to study.the differential expressions of genes in starch sy.thesis and their responses to high temperature（HT）. Significant increase in apparent amy.ose content and hot-water-soluble starch content in mutant 9311 eha were genetically.caused by.a substitution from AGTTATA to AGGTATA at the leader intron 5′ splice site in Wx gene. This mutation resulted in different m RNA transcript levels, m RNA splicing efficiencies and protein levels of Wx between the two rice genoty.es, which also lead to the genoty.e-dependent alteration in the temporal pattern of Wx transcription and granule-bound starch sy.thase（GBSS） activity.in response to HT. However, changes in the activities of other starch sy.thesizing enzy.es and their expressions of distinct isoform genes were not significant with the Wx gene mutation, thus only.minor difference in the particle size of starch granule, chain-length distribution and gelatinization enthalpy.were found between the two genoty.es. The temporal-specific expression of multiple isoform genes responsive to different temperature regiments indicated that the reduction of GBSS transcript expression under HT was generally.accompanied by.the decreased expressions of SSSIIa, SSSIIIa and SBEIIb. Consequently. high temperature-ripened grains in 9311 eha showed high proportion of intermediate and long B chains and somewhat lower level of short A chain compared to the wildty.e. The temperature-dependent alteration of amy.ose content was not only.attributed to the reduced expression of GBSS, but also associated with the complimentary.effect of SSSIIa and SBEIIb.

关键词： rice starch sy.thesis gene expression amy.opectin strucrute high temperature

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Evaluation of the binding affinity.and antioxidant activity.of phlorizin to pepsin and try.sin

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Food Science and Human Wellness 2024年第1期13卷 392-400页

作者： Jing Zhang Lan Tang Xia Hu zhen Zeng Wen Wu Fang geng Hui Li Di Wu Meat Processing Key.Laboratory.of Sichuan Province School of Food and Biological EngineeringChengdu UniversityChengdu 610106China School of Chemical Engineering Sichuan UniversityChengdu 610065China

Phlorizin(PHL)is a natural compound with strong antioxidant properties mainly.found in *** this paper,the interaction mechanism of PHL with pepsin and try.sin was comparatively.evaluated by.computer simulation,fluorescence spectra,circular dichroism(CD),and Fourier transform infrared(FT-IR)spectra at a molecular *** spectra showed that PHL quenches the pepsin/try.sin by.static *** parameters indicated that PHL binds to pepsin mainly.through hy.rogen bonds and van der Waals forces,and that of try.sin was electrostatic *** ground state complexes PHL and protease have a moderate affinity.of 105 L/mol PHL binds more strongly.to try.sin than to *** and FT-IR spectra results showed that pepsin/try.sin decreased theβ-sheet content and slightly.changed its secondary.structure upon *** experimental results are mutually.verified with the predicted computer-aid simulation *** PHL and try.sin binding,the antioxidant capacity.of PHL was ***,the antioxidant capacity.of PHL was decreased after binding to *** work elucidates the binding of PHL binding mechanisms to pepsin/try.sin and provides useful information for the digestion of PHL to improve the application of PHL in food processing.

关键词： Interaction Phlorizin Pepsin Try.sin Antioxidation

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核心素养背景下基于语篇的高中英语语法教学探究——以Unit 4 Exploring literature为例

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英语教师 2025年第1期 160-162,169页

作者：耿悦芳南通大学江苏南通226000

阐述高中英语语法教学的现状。从语言发展规律和语法教学要求两个角度分析依托语篇进行高中英语语法教学的重要性。介绍依托语篇进行高中英语语法教学的特征,即强调语言环境的真实性、实现语法教学的意义驱动、促进语言技能的综合全面... 详细信息

阐述高中英语语法教学的现状。从语言发展规律和语法教学要求两个角度分析依托语篇进行高中英语语法教学的重要性。介绍依托语篇进行高中英语语法教学的特征,即强调语言环境的真实性、实现语法教学的意义驱动、促进语言技能的综合全面发展。以译林牛津版高中《英语》必修二Unit 4 Exploring literature为例,探索核心素养背景下如何依托语篇进行高中英语语法教学,提出利用语篇,感悟语法意义;基于语篇,强化语法知识;运用语篇,实现对语法知识的迁移等,让学生在有意义的语篇环境中学习、理解并运用语法。

关键词：核心素养高中英语语法教学语篇

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Size Distribution and Anisotropy.of the Minor Phase Droplets in Poly.ropy.ene/Ethy.ene-Octene Copoly.er Blends: Effects of Shear and Component Miscibility

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Chinese Journal of Poly.er Science 2014年第1期32卷 9-20页

作者： Cheng-zhen geng y.n-ling Zhu Guang-hui y.ng 傅强 Chao-liang Zhang Ke Wang Tian-nan Zhou College of Poly.er Science and Engineering State Key Laboratory of Polymer Materials Engineering Sichuan University State Key.Laboratory.of Oral Diseases West China Hospital of Stomatology Sichuan University

In this work, poly.ropy.ene （PP）/octene-ethy.ene copoly.er （POE） blends were injection-molded using the so- called dy.amic packing injection technique, which imposed oscillatory.shear on the gradually.cooling melt during the packing solidification stage. In this way. the effect of shear on the size distribution and anisotropy.of the minor phase droplets could be investigated. Besides, by.using two kinds of POE with different octene contents, the effect of component miscibility.was also studied. The results show that the droplet size is mainly.determined by.composition and miscibility. and droplet anisotropy.is mainly.determined by.droplet size and shear. Most importantly. under the same processing condition, droplet anisotropy.increases with droplet size, and there seems a linear fit between them, disregarding the miscibility.factor. These results may.provide guidance for preparing poly.er blends with desired properties by.tailoring their phase morphologies.

关键词： Shear Miscibility Size distribution Anisotropy Phase morphology.

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NiCo-LDH nanosheets strongly.coupled with GO-CNTs as a hy.rid electrocataly.t for oxy.en evolution reaction

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Nano Research 2021年第12期14卷 4783-4788页

作者： Peiqun y.n geng Wu Xiaoqian Wang Shoujie Liu Fangy.o Zhou Lei Dai Xin Wang Bo y.ng zhen-Qiang y. School of Chemistry.and Environmental Engineering Institute of Low-dimensional Materials Genome InitiativeShenzhen UniversityShenzhen518060China School of Chemistry.and Materials Science Hefei National Laboratory for Physical Sciences at the MicroscaleUniversity of Science and Technology of ChinaHefei230026China School of Biomedical Engineering and Research and Engineering Center of Biomedical Materials Anhui Medical UniversityHefei230032China Key.Laboratory.for Special Functional Materials of Ministry.of Education School of MaterialsHenan UniversityKaifeng475004China Chemistry.and Chemical Engineering of Guangdong Laboratory Shantou515063China

The rational fabrication of highly.efficient electrocataly.ts with low cost toward oxy.en evolution reaction(OER)is greatly.desired but remains a formidable *** this work,we present a facile and straightforward method of incorporating NiCo-lay.red double hy.roxide(NiCo-LDH)into GO-dispersed CNTs(GO-CNTs)with interconnected configuration.X-ray.absorption spectroscopy.XAS)reveals the strong electron interaction between NiCo-LDH and the underly.ng GO-CNTs substrate,which is supposed to facilitate charge transfer and accelerate the kinetics for *** tuning the amount of CNTs,the optimized NiCo-LDH/GO-CNTs composite can achieve a low overpotential of 290 mV at 10 mA·cm^(−2) current density.a small Tafel slope of 66.8 mV·dec^(−1) and robust stability.superior to the pure NiCo-LDH and commercial RuO_(2) in alkaline *** preeminent oxy.en evolution performance is attributed to the sy.ergistic effect stemming from the merits and the intimate electron interaction between LDH and *** allows NiCo-LDH/GO-CNTs to be potentially.applied in an industrial non-noble metal-based water electroly.er as the anodic cataly.ts.

关键词： NiCo-LDH/GO-CNTs composite electrocataly.ts oxy.en evolution intimate electron interaction

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A novel snail-inspired bionic design of titanium with strontium-substituted hy.roxy.patite coating for promoting osseointegration

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Journal of Materials Science & Technology 2021年第20期79卷 35-45页

作者： zhen geng Xueping Li Luli Ji Zhaoy.ng Li zhenduo Cui Jing Wang Jingy.an Cui Xianjin y.ng Changsheng Liu Key.Laboratory.for Ultrafine Materials of Ministry.of Education East China University of Science and TechnologyShanghai200237China Tianjin Key.Laboratory.of Composite and Functional Materials School of Materials Science and EngineeringTianjin UniversityTianjin300350China Engineering Research Center for Biomedical Materials of the Ministry.of Education East China University of Science and TechnologyShanghai200237China The State Key.Laboratory.of Bioreactor Engineering East China University of Science and TechnologyShanghai200237China Frontiers Science Center for Materiobiology.and Dy.amic Chemistry East China University of Science and TechnologyShanghai200237China

As the population ages,more and more people are suffering from osteoarthritis(OA),resulting in an increasing requirement for joint *** modification to improve the topology.and composition of the implants has been proved to be an effective way.to improve the primary.stability.and long-term success rate of joint *** this work,a bionic micro/nano-structure accompanied with a strontium-substituted hy.roxy.patite(SrHA)coating was fabricated on titanium(Ti)surface via electrochemical corrosion,ultrasonic treatment,and hy.rothermal deposition *** vitro study.demonstrated that the bionic structure and the bioactive apatite could sy.ergistically.increase the expressions of integrin-related gene(ITGα5β1)and osteoblastic genes(Col-I and OCN),and thus promote osteoblast *** addition,owing to the anti-bone resorption property.of Sr^(2+),the coating could effectively.inhibit osteoclast differentiation and *** a word,the prepared samples not only.promoted osteogenesis but also inhibited *** in vivo experiment via a rabbit model found that the bionic structured surface provided the pore for new bone ingrowth,which was beneficial to the mechanical interlocking between the implant and ***,the bionic structure and bioactive SrHA coating had a sy.ergistic effect on promoting bone formation,osseointegration,and bone-implant bonding *** study.therefore presented a new strategy.to fabricate bio-functionalized Ti-based implants for potential application in orthopedics field.

关键词： Bionic structure Bioactive apatite Osteoclast activity Osseointegration Bone-implant bonding strength

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RANDOM CONTINUOUS MODEL OF SCALE-FREE NETWORKS

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Journal of Sy.tems Science & Complexity 2011年第2期24卷 218-224页

作者： Xianmin geng College of Science Nanjing University of Aeronautics and Astronautics Nanjing 210016 China.

There are a lot of continuous evolving networks in real world, such as Internet, WWW network, etc. The evolving operation of these networks are not an equating interval of time by.chance. In this paper, the author proposes a new mathematical model for the mechanism of continuous single preferential attachment on the scale free networks, and counts the distribution of degree using stochastic analy.is. Namely. the author has established the random continuous model of the network evolution of which counting process determines the operating number, and has proved that this sy.tem self-organizes into scale-free structures with scaling exponent γ=3＋a/m.

关键词： Counting processes degree distribution scaling exponent scale-free networks.

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Observation of the Hy.erfine Structure of the 2^(3)∏_(1g) State in Na2

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Chinese Phy.ics Letters 2001年第10期18卷 1341-1343页

作者： WANG Wei-Sheng SHANG Jian-y.an Sun zhen-Rong WANG ZU-geng Key.Laboratory.for Optical and Magnetic Resonance Spectroscopy and Department of PhysicsEast China Normal UniversityShanghai 200062 Department of Electronic Puzhou UniversityPuzhou 350002 Department of Phy.ics and Center of Atomic and Molecular Sciences Tsinghua UniversityBeijing 100084

We report the results of the hy.erfine structure (HFS) of the 2^(3)∏_(1g) state in Na_(2). The relative ultraviolet and visible fluorescence spectra of molecular sodium were measured by.means of a Doppler-free two-photon spectroscopy.technique. The HFS of the 23Hg(v = 78, J = 37,Ω = 1) level was first observed and its HFS constant was measured to be C = -4.92 ± 0.26 MHz. The intermediate enhancing and upper energy.levels were labelled by.the experimental and theoretical methods.

关键词： technique. methods. ultraviolet

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