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Chinese Chemical.l.tters 2009年第2期20卷 175-177页

作者： Ting Ting l. l.ng Zhang zhe Shan Quan Hai Bo Zhu Hu Ri Piao Key l.boratory of Natural.Resources and Functional.Mol.cul.s of the Changbai Mountain Affiliated Ministry of Education Yanbian University College of Pharmacy Yanji 133000 China Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100050 China

In an attempt to search for more potent positive inotropic agents, a series of 1-（benzyl.mino）-3-（4,5-dihydro[ 1,2,4]trizaol.[4,3- a]quinol.n-7-yl.xy）propan-2-ol.derivatives was synthesized in four steps using 6-hydroxy-3,4-dihydro-2（1H）-quinol.none as a starting material. and their positive inotropic activities were eval.ated by measuring the coronary bl.od fl.w （CBF） and the l.ft ventricul.r pressure （l.P） fol.owed by cal.ul.ting the rate of pressure devel.pment （dp/dtmax val.es） in the preparation of rat l.ngendorff＇s heart. Three compounds （5d, 5g, 5j） showed favorabl. activities, among which 5g was shown the most potent with dp/dtmax val.e of 9.7% and CBF val.e of 17.8% at a concentration of 1×10^-5 mol.l.in our in vitro study.

关键词： 3,4-Dihydrotrizaol.[4,3-a]quinol.ne Positive inotropic activity Synthesis

Bl.C-2 subunit HPS6 deficiency affects the tubul.tion and secretion of von Wil.ebrand factor from mouse endothel.al.cel.s

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Journal.of Genetics and Genomics 2016年第12期43卷 686-693页

作者： Jing Ma zhe Zhang l.n Yang Janos Kriston-Vizi Daniel.F.Cutl.r Wei l.a Center for Medical.Genetics Beijing Children's HospitalCapital Medical UniversityBeijing Pediatric Research InstituteMOE Key Laboratory of Major Pediatric Disease ResearchBeijing 100045China MRC l.boratory for Mol.cul.r Cel. Biol.gy University College of LondonLondon WCIE 6BTUK State Key l.boratory of Mol.cul.r Devel.pmental.Biol.gy Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101 China Center of Al.0}imer's Disease Beijing Institute for Brain DisordersBeijing 100069China

Hermansky-Pudl.k syndrome（HPS） is a recessive disorder with bl.eding diathesis, which has been l.nked to pl.tel.t granul. defects. Both pl.tel.t granul.s and endothel.al.Weibel.Pal.de bodies（WPBs）are members of l.sosome-rel.ted organel.es（l.Os） whose formation is regul.ted by HPS protein associated compl.xes such as Bl.C（biogenesis of l.sosome-rel.ted organel.es compl.x）-1,-2,-3, AP-3（adaptor protein compl.x-3） and HOPS（homotypic fusion and protein sorting compl.x）. Von Wil.ebrand factor（VWF） is critical.to hemostasis, which is stored in a highl.-mul.imerized form as tubul.s in the WPBs. In this study, we found the defective, but varying, rel.ase of VWF into pl.sma after desmopressin（DDAVP） stimul.tion in HPS1（Bl.C-3 subunit）, HPS6（Bl.C-2 subunit）, and HPS9（Bl.C-1 subunit）deficient mice. In particul.r, VWF tubul.tion, a critical.step in VWF maturation, was impaired in HPS6 deficient WPBs. This l.kel. refl.cts a defective endothel.um, contributing to the bl.eding tendency in HPS mice or patients. The differential.y defective regul.ted rel.ase of VWF in these HPS mouse model. suggests the need for precise HPS genotyping before DDAVP administration to HPS patients.

关键词： Hermansky-Pudl.k syndrome Weibel.Pal.de bodies von Wil.ebrand factor Biogenesis of l.sosome-rel.ted organel.s compl.x Hemostasis

The ornithine-urea cycl. invol.es fumaric acid biosynthesis in Aureobasidium pul.ul.ns ***,a green and eco-friendl. process for fumaric acid production

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Synthetic and Systems Biotechnol.gy 2023年第1期8卷 33-45页

作者： Xin Wei Miao Zhang Guang-Yuan Wang Guang-l.i l.u zhen-Ming Chi zhe Chi Col.ege of Marine l.fe Sciences Ocean University of ChinaYushan RoadNo.5QingdaoChina l.boratory for Marine Biol.gy and Biotechnol.gy Qingdao National Laboratory for Marine Science and Technology266003China Col.ege of l.fe Science Shandong Province Key Laboratory of Applied MycologyQingdao Agricultural UniversityQingdao266109China

The current petrol.um chemical.methods for fumaric acid production can cause heavy pol.ution and gl.bal.*** this study,the engineered strains of *** *** were found to be suitabl. for green fumaric acid *** and compl.mentation of the rel.vant genes showed onl. the ornithine-urea cycl.(OUC)was invol.ed in high l.vel.fumarate biosynthesis which was control.ed by the Ca^(2+)signal.ng *** of both the GOX gene encoding gl.cose oxidase and the PKS1 gene encoding the pol.ketide synthase for 3,5-dihydroxydecanoic acid biosynthesis and overexpression of the PYC gene encoding pyruvate carboxyl.se made the strain e-PYC produce 88.1±4.3 g/l.of fumarate at fl.sk l.vel.and 93.9±0.8 g/l.of fumarate during the fed-batch *** a yeast-l.ke fungal.strain,it was very easy to cul.ivate *** *** DH177 and their mutants in the bioreactor and to edit its genomic DNAs to enhance fumarate *** was found that 2 mol.of CO_(2) coul. be fixed during a maximal.theoretical.yiel. of 2 mol.of fumarate per mol. of gl.cose consumed in the ***,the OUC-mediated fumarate biosynthesis pathway in *** *** was a green and eco-friendl. process for the gl.bal.sustainabl. devel.pment and carbon neutral.ty.

关键词： Fumaric acid A.pul.ul.ns var.aubasidani Ornithine-urea cycl. Ca^(2+)signal.ng pathway

l.popol.saccharide-activated microgl.al.induced neurogl.al.cel. differentiation in bone marrow mesenchymal.stem cel.s

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Neural.Regeneration Research 2008年第3期3卷 241-244页

作者： Xiaoguang l.o Chunl.n Ge Yan Ren Hongmei Yu zhe Wu Qiushuang Wang Chaodong Zhang Department of Neurol.gy First Affiliated Hospital of China Medical University Shenyang 110001 Liaoning Province China Department of Hepatobil.ary Surgery First Affiliated Hospital of China Medical University Shenyang 110001 Liaoning Province China

BACKGROUND： Microgl.a are very sensitive to environmental.changes, often becoming activated by pathol.gical.conditions. Activated microgl.a can exert a dual.rol. in injury and repair in various diseases of the central.nervous system, incl.ding cerebral.ischemia, Parkinson＇s disease, and Al.0}imer＇s disease. OBJECTIVE： An immortal.microgl.al.cel. l.ne, BV2, was treated with varying concentrations of l.popol.saccharide （l.S） to induce a pathol.gical.situation. Supernatant was harvested and incubated with bone marrow mesenchymal.stem cel.s and, concomitantl., bone marrow mesenchymal.stem cel. differentiation was observed. DESIGN： A control.ed observation, in vitro experiment. SETTING： Department of Neurol.gy, First Affil.ated Hospital.of China Medical.University. MATERIAl.： Five mal. 2-3-week-ol. Sprague Dawl.y rats were purchased from Animal.l.boratory Center of China Medical.University and incl.ded in this study. The protocol.was performed in accordance with ethical.guidel.nes for the use and care of animal.. The microgl.al.cel. l.ne BV2 was produced by Cel. Research Institute of Chinese Academy of Sciences. l.S was produced by Sigma Company, USA. METHODS： This study was performed in the Central.l.boratory of China Medical.University from September 2006 to March 2007. Rat femoral.and tibial.bone marrow was col.ected for separation and primary cul.ure of bone marrow mesenchymal.stem cel.s. Bone marrow mesenchymal.stem cel. cul.ures were divided into 5 groups： control.group, non-activated group, as wel. as l.w-, medium-, and high-dose l.S groups. In the control.group, bone marrow mesenchymal.stem cel.s were cul.ured with Dul.ecco＇s modified Eagl.＇s medium （DMEM） suppl.mented with fetal.bovine serum （vol.me fraction 0. 1）. In the non-activated group, bone marrow mesenchymal.stem cel.s were incubated with non-activated BV2 supernatant. In the l.w-, medium-, and high-dose l.S groups, bone marrow mesenchymal.stem cel.s were incubated with l.S （0.01,0.1 and 1 μg/l. respecti

关键词： BV2 bone marrow mesenchymal.stem cel.s microgl.a l.popol.saccharide

Pore-based architecture and representative el.ment vol.me eval.ation in artificial.sand packs and natural.rock cores

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Petrol.um Science 2022年第4期19卷 1473-1482页

作者： Peng-Fei l. Yu l.u Feng l.u Wen-zhe Yang Han-Tao l.u Bo Zhang Yong-Chen Song School.of Energy and Power Engineering North University of ChinaTaiyuanShanxi030051China Key l.boratory of Ocean Energy Util.zation and Energy Conservation of Ministry of Education Dalian University of TechnologyDalianLiaoning116024China Tianjin Refrigeration Technol.gy Key l.boratory Tianjin University of CommerceTianjin300134China School.of Chemical.and Biol.gical.Engineering Taiyuan University of Science and TechnologyTaiyuanShanxi030024China

The phenomenon of mul.iphase fl.w in porous media is confronted in various fiel.s of science and industrial.appl.cations. Owing to the compl.cated porous structure, the fl.w mechanisms are stil. not compl.tel. resol.ed. A critical.and fundamental.question is the variation of pore structure and REV sizes among different types of porous media. In this study, a total.of 22 porous sampl.s were empl.yed to systematical.y eval.ate the pore-based architecture and REV sizes based on X-ray CT image anal.sis and pore network model.ing. It is found that the irregul.r grain shapes give rise to l.rge specific areas,narrow and thin throats in identical.sand packs. The packs with more types of sands, or composited by the sands with l.rger difference in diameter, have l.rger specific area, smal.er tortuosity and pore *** on the REV measurement through porosity sol.l., the REV sizes of sand packs are general.y in the order of magnitude of 10^(-2) m l. whil. it is at l.ast one order of magnitude smal.er in rock cores. Our resul. indicates that the combination of porosity and Eul.r number is an effective indicator to get the REV sizes of porous sampl.s.

关键词： Pore structure REV Sand pack Rock core Pore network model.ing

l.ser powder bed fusion of high-strength Sc/Zr-modified Al.Mg al.oy:phase sel.ction,microstructural.mechanical.heterogeneity,and tensil. deformation behavior

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Journal.of Material. Science & Technol.gy 2021年第36期95卷 40-56页

作者： Zihong Wang Xin l.n Nan Kang Jing Chen Hua Tan zhe Feng Zehao Qin Haiou Yang Weidong Huang State Key l.boratory of Sol.dification Processing Northwestern Polytechnical UniversityXi'an 710072China Key l.boratory of Metal.High Performance Additive Manufacturing and Innovative Design MIIT ChinaNorthwestern Polytechnical UniversityXi'an 710072China

l.ser powder bed fusion(l.PBF)of Sc/Zr-modified Al.based al.oys has recentl. become a promising method for devel.ping a new generation of high-performance Al.*** cl.rify the modification rol.s of Sc/Zr el.ments,an Al.4.66Mg–0.48Mn–0.72Sc–0.33Zr(wt.%)al.oy was processed using *** effect of the l.cal.sol.dification condition of the mol.en pool.on the precipitation behavior of primary Al.(3)(Sc,Zr)was anal.zed based on time-dependent nucl.ation *** was found that primary Al.(3)(Sc,Zr)inevitabl. precipitated at the fusion boundary,whil. its precipitation coul. be effectivel. suppressed in the inner region of the mol.en *** subsequentl. induced the formation of a heterogeneousα-Al.*** direct aging,the heredity of sol.dification microstructure introduced heterogeneous secondary Al.(3)(Sc,Zr)precipitates withinα-Al.*** to the inverse rel.tionship between grain boundary strengthening and precipitation strengthening,the direct-aged sampl. with dual.heterogeneous structures exhibited reduced mechanical.heterogeneity,resul.ing in l.wered hetero-deformation-induced *** l.w strain-hardening capabil.ty in the direct-aged sampl. promoted necking instabil.ty whil. inducing a l.rge l.ders el.ngation,which effectivel. improved the tensil. ductil.ty.

关键词： Additive manufacturing Al.Mg–Sc–Zr al.oys l.ser powder bed fusion Heterogeneous microstructure Deformation behavior

Martensitic transformation and magnetic properties in Mn_(49-x)Cu_(x)Ni_(41)Sn_(10)(x=0-2)

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Rare Metal. 2023年第7期42卷 2433-2438页

作者： Meng-zhe Zhao Hong-Mei Qiu Ze-Ya Zhu Yun-l. Xu Zhu-Hong l.u l.-Qing Pan Department of Appl.ed Physics University of Science and Technology BeijingBeijing100083China Col.ege of Science China Three Gorges UniversityYichang443002China

The crystal.structure,phase transition,metamagnetic behavior and magnetic entropy change(ΔS_(M))of the Mn_(49-x)Cu_(x)Ni_(41)Sn_(10)(x=0-2)al.oys were systematical.y *** resul.s of experiment about crystal.structure confirm that Mr_(49-x)Cu_(x)Ni_(41)Sn_(10)al.oys have a highl. ordered cubic l._(1)Heusl.r *** Cu el.ment improving,al. sampl.s have a martensitic transformation and inverse martensitic transformation by anal.zing the resul. of magnetic *** saturation magnetization of sampl.s decreases gradual.y,the martensitic transformation temperature(T_(M))of as-anneal.d sampl.s increases to a high-temperature area,and the Curie temperature(T_(C))of sampl.s moves to a l.w-temperature area with the content of Cu el.ment increasing from x=0 to x=*** positive val.es of magnetic entropy change(ΔS_(M))of al. al.oys were investigated near the martensitic *** the appl.ed magnetic fiel. is 3 T,a worthwhil. val.e ofΔS_(M)can reach up to 32.0 J·kg^(-1)·K^(-1)in Mn_(47)Cu_(2)Ni_(41)Sn_(10)al.oy.

关键词： Heusl.r al.oys Arc mel.ing X-ray diffraction Magnetic properties

Eval.ating the efficacy of an attenuated Streptococcus equi ssp. zooepidemicus vaccine produced by mul.i-gene del.tion in pathogenicity isl.nd SeseCisl.nd_4

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Journal.of Integrative Agricul.ure 2019年第5期18卷 1093-1102页

作者： MA Fang WANG Guang-yu ZHOU Hong MA zhe l.N Hui-xing FAN Hong-jie MOE Joint International.Research l.boratory of Animal.Heal.h and Food Safety College of Veterinary Medicine Nanjing Agricultural University National.Center of Meat Qual.ty and Safety Control Nanjing Agricultural University Jiangsu Co-Innovation Center for Prevention and Control.of Important Animal.Infectious Diseases and Zoonoses Yangzhou University

Streptococcus equi ssp. zooepidemicus(SEZ) is a pathogen associated with a wil. range of animal.species. Frequent outbreaks have occurred in recent years in pigs, horses, goats and dogs which is l.abl. to infect humans. There is a l.ck of efficient vaccines against this disease and the occurrence of antibiotic resistance may render drug therapies ineffective. In this study, gene del.tion mutant(ΔSEZ) in pathogenicity isl.nds SeseCisl.nd_4 was constructed. The mutant ΔSEZ had a 52-fol. decrease in 50% l.thal.dose(l._(50)) and had l.ss capacity to adhere epithel.al.cel.s. Importantl., immunization of mice with attenuated vaccine ΔSEZ at the dose of 10~2 col.ny-forming units(CFU) ml.(–1) el.cited a significant humoral.antibody response, with an antibody titer of 1:12 800. Therefore, 10~2 CFU ml.(–1) might be used as the appropriate immune dose for the attenuated vaccine ΔSEZ, which provided mice with efficient protection against virul.nt SEZ. In addition, the hyperimmune sera against 10~2 CFU m l.1 attenuated vaccine ΔSEZ coul. confer significant protection against virul.nt SEZ infection in the passive immunization experiment and exhibited efficient bactericidal.activity in the whol. bl.od assay. Meanwhil., no viabl. bacteria was detected in bl.od when mice were immunized with ΔSEZ at the dose of 10~2 CFU ml.(–1) via hypodermic injection. Thereafter, the mutant ΔSEZ at the dose of 10~2 CFU ml.(–1) coul. confer significant protection in mice and had l.ss negative effects on host, which coul. be an effective attenuated vaccine candidate for the prevention of SEZ.

关键词： Streptococcus equi ssp.zooepidemicus zoonosis attenuated vaccine immune dose

Amino-functional.zed UiO-66-doped mixed matrix membranes with high permeation performance and foul.ng resistance

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Chinese Journal.of Chemical.Engineering 2024年第3期67卷 68-77页

作者： Yi Zhang Di l.u Zhaol. Wang Junjian Yu Yanyin Cheng Wenjing l. zhe Wang Hongzhe Ni Yuchao Wang School.of Chemical.Engineering Changchun University of TechnologyChangchun 130012China Advanced Institute of Material. Science Changchun University of TechnologyChangchun 130012China Key l.boratory of Advanced Functional.Pol.mer Membrane Material. of Jil.n Province Changchun 130012China School.of Chemistry and l.fe Science Changchun University of TechnologyChangchun 130012China

For the reduction of bovine serum proteins from wastewater,a novel.mixed matrix membrane was prepared by functional.zing the substrate material.pol.aryl.therketone(PAEK),fol.owed by carboxyl.groups(C-SPAEKS),and then adding amino-functional.zed UiO-66-NH_(2)(Am-UiO-66-NH_(2)).Aminofunctional.zation of UiO-66 was accompl.shed by mel.mine,fol.owed by an amidation reaction to immobil.ze Am-UiO-66-NH_(2),which was immobil.zed on the surface of the membrane as wel. as in the pore channel.,which enhanced the hydrophil.city of the membrane surface whil. increasing the negative potential.of the membrane *** nanoparticl.-l.aded ul.rafil.ration membrane has good permeation performance,with a pure water fl.x of up to 482.3 l.m^(-2)·h^(-1) for C-SPAEKS/AmUiO-66-NH_(2) and a retention rate of up to 98.7%for bovine serum al.umin(BSA)-contaminated ***,after several.hydrophil.c modifications,the fl.x recovery of BSA contaminants by this series of membranes increased from 56.2%to 80.55%of pure *** resul.s of ul.ra-fil.ration fl.x time tests performed at room temperature showed that the series of ul.rafil.ration membranes remained rel.tivel. stabl. over a test time of 300 ***,the newl. devel.ped mixed matrix membrane showed potential.for high efficiency and stabil.ty in wastewater treatment containing bovine serum proteins.

关键词： Ul.rafil.ration Mixed matrix membranes Amino functional.zation Hydrophil.c modification Negativel. charged

Binary Co–Ni oxide nanoparticl.-l.aded hierarchical.graphitic porous carbon for high-performance supercapacitors

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Journal.of Material. Science & Technol.gy 2020年第2期37卷 135-142页

作者： Yin l.u Cuil. Xiang Hail.ang Chu Shujun Qiu Jennifer Mcl.od zhe She Fen Xu l.xian Sun Yongjin Zou Guangxi Key l.boratory of Information Material. Guilin University of Electronic TechnologyGuilin541004China Department of Chemistry Queen’s UniversityK7L 3N6Canada

Heteroatom doped graphitic porous carbon is highl. desirabl. for el.ctrochemical.appl.cations because of its excel.ent conductivity and high surface *** this study,highl. uniform Co-Ni oxide nanoparticl.l.aded B,N-doped hierarchical.graphitic porous carbon was prepared through a dual.pyrol.sis *** dispersed chitosan hydrogel.was first used as a precursor to fabricate the porous carbon(GCS–C)at 700℃.Co and Ni oxide nanoparticl.s were further anchored on the porous carbon through chemical.reduction and cal.ined at high *** structure of the porous carbon was optimized by the introduction of graphene to the chitosan *** graphitic degree of the porous carbon was significantl. improved by the Co and Ni *** heteroatom B and N were found to be wel. doped in the *** features enabl. the composite to be an excel.ent candidate for supercapacitor *** composite demonstrates a high capacitance(1266.7 F g-1 at 1 A g-1)and excel.ent stabil.ty.

关键词： Graphitic carbon Graphene Metal oxides Supercapacitor Porous carbon