Lipopolysaccharide-activated microglial-induced neuroglial cell differentiation in bone marrow mesenchymal stem cells

作     者：Xiaoguang Luo Chunlin Ge Yan Ren Hongmei Yu Zhe Wu Qiushuang Wang Chaodong Zhang 

作者机构：Department of Neurology First Affiliated Hospital of China Medical University Shenyang 110001 Liaoning Province China Department of Hepatobiliary Surgery First Affiliated Hospital of China Medical University Shenyang 110001 Liaoning Province China 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2008年第3卷第3期

页      面：241-244页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071006[理学-神经生物学] 

主　　题：BV2 bone marrow mesenchymal stem cells microglia lipopolysaccharide 

摘      要：BACKGROUND： Microglia are very sensitive to environmental changes, often becoming activated by pathological conditions. Activated microglia can exert a dual role in injury and repair in various diseases of the central nervous system, including cerebral ischemia, Parkinson＇s disease, and Alzheimer＇s disease. OBJECTIVE： An immortal microglial cell line, BV2, was treated with varying concentrations of lipopolysaccharide （LPS） to induce a pathological situation. Supernatant was harvested and incubated with bone marrow mesenchymal stem cells and, concomitantly, bone marrow mesenchymal stem cell differentiation was observed. DESIGN： A controlled observation, in vitro experiment. SETTING： Department of Neurology, First Affiliated Hospital of China Medical University. MATERIALS： Five male 2-3-week-old Sprague Dawley rats were purchased from Animal Laboratory Center of China Medical University and included in this study. The protocol was performed in accordance with ethical guidelines for the use and care of animals. The microglial cell line BV2 was produced by Cell Research Institute of Chinese Academy of Sciences. LPS was produced by Sigma Company, USA. METHODS： This study was performed in the Central Laboratory of China Medical University from September 2006 to March 2007. Rat femoral and tibial bone marrow was collected for separation and primary culture of bone marrow mesenchymal stem cells. Bone marrow mesenchymal stem cell cultures were divided into 5 groups： control group, non-activated group, as well as low-, medium-, and high-dose LPS groups. In the control group, bone marrow mesenchymal stem cells were cultured with Dulbecco＇s modified Eagle＇s medium （DMEM） supplemented with fetal bovine serum （volume fraction 0. 1）. In the non-activated group, bone marrow mesenchymal stem cells were incubated with non-activated BV2 supernatant. In the low-, medium-, and high-dose LPS groups, bone marrow mesenchymal stem cells were incubated with LPS （0.01,0.1 and 1 μg/L, respecti