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乌梢蛇醇溶蛋白质提取工艺优化及其指纹图谱表征

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中成药 2024年第7期46卷 2177-2181页

作者：谢超宋美营王巧宇张林松刘莹曹云飞闻崇炜镇江市食品药品监督检验中心江苏镇江212050 江苏大学药学院江苏镇江212013

目的优化乌梢蛇醇溶蛋白质提取工艺,并对其进行指纹图谱表征。方法在单因素试验基础上,以料液比、乙醇体积分数、提取温度、提取时间为影响因素,提取率为评价指标,响应面法优化提取工艺,再进行FTIR、SDS-PAGE分析。结果最佳条件为料液比... 详细信息

目的优化乌梢蛇醇溶蛋白质提取工艺,并对其进行指纹图谱表征。方法在单因素试验基础上,以料液比、乙醇体积分数、提取温度、提取时间为影响因素,提取率为评价指标,响应面法优化提取工艺,再进行FTIR、SDS-PAGE分析。结果最佳条件为料液比1∶43.15,乙醇体积分数46.35%,提取温度37.53℃,提取时间250min,提取率为0.4072%。醇溶蛋白质在4000~400cm-1波数范围内有10个吸收峰,并且其种类丰富,分子量在11.19~158.10kDa范围内,其中26.48、19.67kDa为指纹图谱主峰。结论本实验可为乌梢蛇开发利用及分析鉴定提供依据。

关键词：乌梢蛇醇溶蛋白质提取工艺指纹图谱响应面法 FTIR SDS-PAGE

Real-time Tire Parameters Observer for Vehicle Dynamics Stability Control

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Chinese Journal of Mechanical Engineering 2010年第5期23卷 620-626页

作者： LI Liang LI Hongzhi zhang Xiaolong HE lin song Jian State Key Laboratory of Automotive Safety and Energy Tsinghua University Beijing 100084 China

The performance of the vehicle dynamics stability control system（DSC） is dominated by the accurate estimation of tire forces in *** characteristics of tire forces are determined by tire dynamic states and parameters,which vary in an obviously large scope along with different working ***,there have been many methods based on the nonlinear observer to estimate the tire force and dynamic parameters,but they were only used in off-line analysis because of the computation complexity and the dynamics differences of four tires in the steering maneuver conditions were not considered *** paper develops a novel algorithm to observe tire parameters in real-time controller for *** algorithm is based on the sensor-fusion technology with the signals of DSC sensors,and the tire parameters are estimated during a set of maneuver *** calibrated tire parameters in the control cycle are treated as the elementary states for vehicle dynamics observation,in which the errors between the calculated and the measured vehicle dynamics are used as the correcting factors for the tire parameter observing *** test process with a given acceleration following a straight line is used to validate the estimation method of the longitudinal stiffness;while the test process with a given steering angle is used to validate the estimated value of the cornering *** ground test result shows that the proposed algorithm can estimate the tire stiffness accurately with an acceptable computation cost for real-time controller only using DSC sensor *** proposed algorithm can be an efficient algorithm for estimating the tire dynamic parameters in vehicle dynamics stability control system,and can be used to improve the robustness of the DSC controller.

关键词： tire longitudinal stiffness cornering stiffness vehicle dynamics stability

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响应面法优化金钱白花蛇蛋白质的酸提工艺

粮食与油脂 2024年第3期37卷 68-71,81页

作者：母璇刘梦李靓颖张峻豪张林松闻崇炜江苏大学药学院江苏镇江212013 镇江市食品药品监督检验中心江苏镇江212050

以金钱白花蛇为原料,通过酸提法提取金钱白花蛇中的蛋白质,以蛋白质得率为指标,在单因素试验基础上通过响应面法优化提取工艺,并对提取物进行指纹图谱分析。结果表明:最佳酸提条件为醋酸钠浓度0.20mol/L、料液比1∶40(g/mL)、提取时间6 ... 详细信息

以金钱白花蛇为原料,通过酸提法提取金钱白花蛇中的蛋白质,以蛋白质得率为指标,在单因素试验基础上通过响应面法优化提取工艺,并对提取物进行指纹图谱分析。结果表明:最佳酸提条件为醋酸钠浓度0.20mol/L、料液比1∶40(g/mL)、提取时间6 h、提取温度45℃,在此条件下蛋白质得率为3.92%。指纹图谱分析表明,酸提法所得金钱白花蛇的蛋白质种类较丰富。

关键词：金钱白花蛇蛋白质酸提指纹

QTL-seq analysis of the seed size trait in grape provides new molecular insights on seedlessness

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Journal of Integrative Agriculture 2022年第10期21卷 2910-2925页

作者： WANG Li zhang song-lin JIAO Chen LI Zhi LIU Chong-huai WANG Xi-ping State Key Laboratory of Crop Stress Biology in Arid Areas/College of Horticulture Northwest A&F UniversityYangling 712100P.R.China College of Horticulture Hebei Agricultural UniversityBaoding 071001P.R.China Zhengzhou Fruit Research Institute Chinese Academy of Agricultural SciencesZhengzhou 450000P.R.China Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China of Ministry of Agriculture and Rural Affairs Northwest A&F UniversityYangling 712100P.R.China

Seedlessness in grape(Vitis vinifera)is an important commercial trait for both the fresh and drying ***,despite numerous studies,the mechanisms and key genes regulating grape seedlessness are mostly *** this study,we sequenced the genomes of the *** seeded cultivar‘Red Globe’,the seedless cultivar‘Centennial Seedless’,and the derived *** single nucleotide polymorphisms(SNPs)were identified by genome sequencing and analyzed using published transcriptome *** SNPs occurred in genes related to seed development,which were identified as protein kinases,transcription factors,and cytochrome P450 s and showed differential expression during ovule development in both seeded and seedless *** nonsynonymous SNP-associated genes were mainly involved in biological processes such as hormone balance,seed coat and endosperm development,reproductive organ development,oxidation and reduction,senescence and cell death.A potential quantitative trait locus(QTL)region associated with seed size was characterized based on the SNP-index,and expression analysis of candidate genes in the QTL region during ovule development in multiple seeded and seedless grape cultivars were *** SNPs were further subjected to SNa Pshot analysis and one SNP in G8 showed 67.5%efficiency in the grape progeny ***,the data obtained in this study shed light on the differences in seed development between seeded and seedless progeny at the genomic level,which provides valuable resources for future functional studies and grape breeding.

关键词： grape genome QTL-seq seedless SNP

Integrability and solutions of a nonsymmetric discrete Korteweg-de Vries equation

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Communications in Theoretical Physics 2024年第2期76卷 45-50页

作者： Maebel Mesfun Da-jun zhang song-lin Zhao Department of Mathematics Shanghai UniversityShanghai 200444China Newtouch Center for Mathematics of Shanghai University Shanghai 200444China Department of Applied Mathematics Zhejiang University of TechnologyHangzhou 310023China

In this paper,we present Lax pairs and solutions for a nonsymmetric lattice equation,which is a torqued version of the lattice potential Korteweg-de Vries *** nonsymmetric equation is special in the sense that it contains only one spacing parameter but consists of two consistent cubes with other integrable lattice *** such a multidimensionally consistent property we are able to derive its two Lax pairs and also construct solutions using B?cklund transformations.

关键词： discrete Korteweg-de Vries equation nonsymmetric multi-dimensional consistency Backlund transformation solution

Capacity Market Mechanism Design,Considering Network Optimization and Energy Transition

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CSEE Journal of Power and Energy Systems 2024年第4期10卷 1676-1687页

作者： Nan Shang Yihang song Xiang zhang You lin China Southern Power Grid Co. Ltd.Guangzhou 510663China Southern Methodist University Junkins 3086251 Airline Road DallasTX 75205US

The coordination of enrgy transition,fixed cost recovery,and sufficient generation supply leads to a new challenge for a traditional capacity market ***,in order to better match network expansion at the same time,it is crucial to redesign the capacity market mechanism considering system *** this paper,a novel capacity market mechanism is proposed considering spot market operations,network expansion,and energy transition,which can minimize the total cost of capacity investment,network expansion,and generation operations,while satisfying the energy transition constraints and topology ***,the capacity market mechanism co-ordinated with spot market operations is illustrated,in which the energy transition and network constraints are ***,a bi-level optimization model is established where the trade organizers minimize the total cost of both investment and operations,subject to the spot power market simultaneously minimizing the local dispatching *** numerical results of a test system show that more economical capacity portfolios can be obtained by constructing reasonable transmission lines,thereby obtaining a more optimal market cost.A detailed multi-scenario simulation is further analyzed to verify the effectiveness of the proposed market mechanism.

关键词： Capacity market energy transition network expansion spot market operation

Photostriction effect and electric properties of La-doped PMN-PT transparent ferroelectric ceramics

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International Journal of Smart and Nano Materials 2024年第2期15卷 296-311页

作者： Zhiqiang song Zhuo zhang Yirong Yu Dabin lin Department of Automation and Robotics Engineering School of AutomationWuxi UniversityWuxiChina School of Optoelectronic Engineering Xian Technological UniversityXi'anChina

Ferroelectric ceramics display a remarkable photostriction effect,which could facilitate the prevention of electromagnetic interference and promote the integration and miniaturization of actuator systems,indicating potential applications in glim-mer *** this work,highly transparent La-doped 0.9Pb(Mg1/3Nb2/3)O3-0.1PbTiO3(0.9PMN-0.1PT)ceramicswere synthesized using a solid-state reaction method in an oxyge-nated *** and SEM were used to analyze the phase structure of the dense samples,which displayed a pure perovskite structure,with no trace of a pyrochlore *** La doping,the phase transitiontemperature shows a decreasing trend,and its dielectric constant could reach up to 20,*** P-E loop of the samples was gradually refined into a straight line with an increase in the doping *** La doping,the transparency of the 0.9PMN-0.1PT cera-mics was significantly enhanced and reached up to 61%at the near-infrared ***,the photogenerated voltage and deformation of the La-doped 0.9PMN-0.1PT ceramics were investigated under ultraviolet light of various *** 20 s of irradiation at an intensity of 800 mW/cm²,the max-imum voltage and deformation,with values of 40 V and 250 nm,respectively,were both found in the 1%La-doped 0.9PMN-0.1PT ceramics.

关键词： Photostriction ferroelectric Raman piezoelectric

Global Spherically Symmetric Solutions for a Coupled Compressible Navier–Stokes/Allen–Cahn System

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Acta Mathematica Sinica,English Series 2024年第8期40卷 2037-2064页

作者： Chang Ming song Jian lin zhang Yuan Yuan WANG Department of Applied Mathematics School of Mathematics and Information ScienceZhongyuan University of TechnologyZhengzhou 450007P.R.China

In this paper,we consider the global spherically symmetric solutions for the initial boundary value problem of a coupled compressible Navier–Stokes/Allen–Cahn system which describes the motion of two-phase viscous compressible *** prove the existence and uniqueness of global classical solution,weak solution and strong solution under the assumption of spherically symmetry condition for initial dataρ0 without vacuum state.

关键词： Diffuse interface model global solutions Navier-Stokes equations Allen-Cahn equation spherically symmetry

Rheological Behaviors of Polymers with Nanoparticles Tethered at Each End

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Chinese Journal of Polymer Science 2024年第3期42卷 400-406,I0009页

作者： song-Qi zhang Wen-Qing Wang Jia-Ping lin Li-Quan Wang Shanghai Key Laboratory of Advanced Polymeric Materials Key Laboratory for Ultrafine Materials of Ministry of EducationSchool of Materials Science and EngineeringEast China University of Science and TechnologyShanghai 200237China

The polymer with nanoparticles tethered at each end is a unique model for unraveling the effect of chain ends on the polymer dynamics. We investigated the rheological behavior of this kind of polymer by using nonequilibrium molecular dynamics simulation. The effect of polymer lengths and nanoparticle radii on the complex moduli and viscosity was examined. The dependence of complex moduli on the frequency becomes less pronounced as the polymer is short or the nanoparticle is large. The shear thinning behavior was revealed for these systems, and the scaling exponent of complex viscosity with respect to the frequency was found to change from-1/2 to-3/4 as the polymer shortens or the nanoparticle enlarges. The rheological behavior was further explained by analyzing the mean square distance of nanoparticles. The simulation results were compared with the existing experimental finding, showing an agreement. The work provides information for understanding the chain end effect on polymer rheology.

关键词： Polymer rheology Modulus Viscosity Molecular dynamics