QTL-seq analysis of the seed size trait in grape provides new molecular insights on seedlessness

作     者：WANG Li ZHANG Song-lin JIAO Chen LI Zhi LIU Chong-huai WANG Xi-ping WANG Li;ZHANG Song-lin;JIAO Chen;LI Zhi;LIU Chong-huai;WANG Xi-ping

作者机构：State Key Laboratory of Crop Stress Biology in Arid Areas/College of HorticultureNorthwest A&F UniversityYangling 712100P.R.China College of HorticultureHebei Agricultural UniversityBaoding 071001P.R.China Zhengzhou Fruit Research InstituteChinese Academy of Agricultural SciencesZhengzhou 450000P.R.China Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China of Ministry of Agriculture and Rural AffairsNorthwest A&F UniversityYangling 712100P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2022年第21卷第10期

页      面：2910-2925页

核心收录：

学科分类：09[农学] 0902[农学-园艺学] 090201[农学-果树学] 

基　　金：supported by the National Natural Science Foundation of China (U1603234) the Program for Innovative Research Team of Grape Germplasm Resources and Breeding of Shaanxi, China (2013KCT25) the Chinese Universities Scientific Fund (Z109021571 and 2452019170) the Natural Science Foundation of Hebei, China (C2021204146) the Scientific Research Program of Hebei Educational Commission, China (QN2020232) 

主　　题：grape genome QTL-seq seedless SNP 

摘      要：Seedlessness in grape(Vitis vinifera)is an important commercial trait for both the fresh and drying ***,despite numerous studies,the mechanisms and key genes regulating grape seedlessness are mostly *** this study,we sequenced the genomes of the *** seeded cultivar‘Red Globe’,the seedless cultivar‘Centennial Seedless’,and the derived *** single nucleotide polymorphisms(SNPs)were identified by genome sequencing and analyzed using published transcriptome *** SNPs occurred in genes related to seed development,which were identified as protein kinases,transcription factors,and cytochrome P450 s and showed differential expression during ovule development in both seeded and seedless *** nonsynonymous SNP-associated genes were mainly involved in biological processes such as hormone balance,seed coat and endosperm development,reproductive organ development,oxidation and reduction,senescence and cell death.A potential quantitative trait locus(QTL)region associated with seed size was characterized based on the SNP-index,and expression analysis of candidate genes in the QTL region during ovule development in multiple seeded and seedless grape cultivars were *** SNPs were further subjected to SNa Pshot analysis and one SNP in G8 showed 67.5%efficiency in the grape progeny ***,the data obtained in this study shed light on the differences in seed development between seeded and seedless progeny at the genomic level,which provides valuable resources for future functional studies and grape breeding.