检索结果-南通市图书馆

The Journal of Biomedical Research 2017年第3期31卷 248-255页

作者： Xunxian Liu zemin yao Department of Biochemistry Microbiology and ImmunologyOttawa Institute of Systems BiologyUniversity of OttawaOttawaOntario K1H 8M5Canada

Our recent studies with cultured retinal pigment epithelium cells suggested that overexpression of interleukin 17 receptor C（IL-17RC）,a phenomenon observed in peripheral blood and chorioretinal tissues with age-related macular degeneration（AMD）,was associated with altered activation of phosphatidylinositide 3-kinase（PI3K）,Akt,and glycogen synthase kinase 3（GSK3）.We wondered whether or not altered PI3 K,Akt,and GSK3 activities could be detected in peripheral blood mononuclear cells（PBMC） obtained from AMD patients.In the patients＇ PBMC,absent or reduced serine-phosphorylation of GSK3α or GSK3β was observed,which was accompanied with increased phosphorylation of GSK3 substrates（e.g.CCAAT enhancer binding protein a,insulin receptor substrate 1,and TAU）,indicative of enhanced GSK3 activation.In addition,decreased protein mass of PI3K85α and tyrosinephosphorylation of PI3K50α was present in PBMC of the AMD patients,suggesting impaired PI3 K activation.Moreover,abnormally lowered molecular weight forms of Akt and GSK3 were detected in PBMC of the AMD patients.These data demonstrate that despite the presence of high levels of IL-17 RC,Wnt-3a and vascular endothelial growth factor,the PI3K/Akt/GSK3 signaling pathway is insensitive to these stimuli in PBMC of the AMD patients.Thus,measurement of PI3K/Akt/GSK3 expression and activity in PBMC may serve as a surrogate biomarker for AMD.

关键词： phosphatidylinositide 3-kinase （PI3K） protein kinase B （PKB or Akt） glycogen synthase kinase 3（GSK3） age-related macular degeneration （AMD） peripheral blood mononuclear cells （PBMC）

Re-evaluating the role of epithelial-mesenchymal-transition in cancer progression

在线全文

学校读者我要写书评

暂无评论

The Journal of Biomedical Research 2018年第2期32卷 81-90页

作者： Andrew Sulaiman zemin yao Lisheng Wang Department of Biochemistry Microbiology and Immunology Faculty of Medicine University of Ottawa China-Canada Centre of Research for Digestive Diseases Ottawa Institute of Systems Biology University of Ottawa Regenerative Medicine Program Ottawa Hospital Research Institute

Epithelial-mesenchymal transition（EMT） and mesenchymal-epithelial transition（MET） are essential for embryonic development and also important in cancer progression. In a conventional model, epithelial-like cancer cells transit to mesenchymal-like tumor cells with great motility via EMT transcription factors; these mesenchymallike cells migrate through the circulation system, relocate to a suitable site and then convert back to an epithelial-like phenotype to regenerate the tumor. However, recent findings challenge this conventional model and support the existence of a stable hybrid epithelial/mesenchymal（E/M） tumor population. Hybrid E/M tumor cells exhibit both epithelial and mesenchymal properties, possess great metastatic and tumorigenic capacity and are associated with poorer patient prognosis. The hybrid E/M model and associated regulatory networks represent a conceptual change regarding tumor metastasis and organ colonization. It may lead to the development of novel treatment strategies to ultimately stop cancer progression and improve disease-free survival.

关键词： Epithelial-mesenchymal transition（EMT） mesenchymal-epithelial transition（MET） hybrid EMT/MET cancer metastasis

Postprandial hyperglycemia and postprandial hypertriglyceridemia in type 2 diabetes

在线全文

学校读者我要写书评

暂无评论

The Journal of Biomedical Research 2019年第1期33卷 1-16页

作者： Toru Hiyoshi Mutsunori Fujiwara zemin yao Division of Diabetes and Endocrinology Department of Internal Medicine Japanese Red Cross Medical Center Department of Laboratory Medicine Japanese Red Cross Medical Center Department of Biochemistry Microbiology and Immunology Ottawa Institute of Systems BiologyUniversity of Ottawa

Postprandial glucose level is an independent risk factor for cardiovascular disease that exerts effects greater than glucose levels at fasting state, whereas increase in serum triglyceride level, under both fasting and postprandial conditions, contributes to the development of arteriosclerosis. Insulin resistance is a prevailing cause of abnormalities in postabsorptive excursion of blood glucose and postprandial lipid profile. Excess fat deposition renders a vicious cycle of hyperglycemia and hypertriglyceridemia in the postprandial state, and both of which are contributors to atherosclerotic change of vessels especially in patients with type 2 diabetes mellitus. Several therapeutic approaches for ameliorating each of these abnormalities have been attempted, including various antidiabetic agents or new compounds targeting lipid metabolism.

关键词： postprandial hyperglycemia postprandial hypertriglyceridemia Type 2 diabetes mellitus atherosclerosis

Efficacy of polyphenolic ingredients of Chinese herbs in treating dyslipidemia of metabolic syndromes

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Medicine 2014年第3期12卷 135-146页

作者： zemin yao Li Zhang Guang Ji Department of Biochemistry Microbiology and ImmunologyOttawa Institute of System BiologyUniversity of Ottawa Institute of Digestive Diseases Shanghai University of Traditional Chinese Medicine E-institute of Shanghai Municipal Education Commission Shanghai University of Traditional Chinese Medicine

There is an increasing interest and popularity of Chinese herbal medicine worldwide, which is accompanied by increasing concerns about its effectiveness and potential toxicity. Several ingredients, such as polyphenolic compounds berberine, flavonoids, and curcumin, have been studied extensively by using various animal models. Effectiveness of treatment and amelioration of metabolic syndromes, including insulin resistance and dyslipidemia, has been demonstrated. This review summarizes the major checkpoints and contributing factors in regulation of exogenous and endogenous lipid metabolism, with particular emphasis centered on triglyceride-rich and cholesterol-rich lipoproteins. Available experimental evidence demonstrating the lipid-lowering effect of berberine, flavonoids and curcumin in cell culture and animal models is compiled, and the strengths and shortcomings of experimental designs in these studies are discussed.

关键词： drugs, Chinese herbal metabolic syndromes dyslipidemias reviews

Expression of human hepatic lipase negatively impacts apolipoprotein A-Ⅰ production in primary hepatocytes from Lipc-null mice

在线全文

学校读者我要写书评

暂无评论

The Journal of Biomedical Research 2014年第3期28卷 201-212页

作者： Michelle Bamji-Mirza Wandong Zhang zemin yao Department of Biochemistry Microbiology & ImmunologyUniversity of Ottawa Department of Pathology and Laboratory Medicine University of Ottawa Human Health Therapeutics Portfolio Life Sciences DivisionNational Research Council Canada Ottawa Institute of Systems Biology University of Ottawa

This study aimed to examine whether expression of human hepatic lipase（hHL） exerted an intracellular effect on hepatic production of apolipoprotein（apo） A-I.The levels of secreted and cell-associated apoA-I were contrasted between primary hepatocytes isolated from Lipc-nuW and C57BL/6 mice,and between Lipc-nuW hepatocytes transfected with either hHL-encoding or control adenovirus.An HSPG-binding deficient hHL protein（hHLmt） was used to determine the impact of cell surface binding on HL action.Accumulation of apoA-I in conditioned media of primary hepatocytes isolated from Lipc-nuW mice was increased as compared to that from C57BL/6 mice.Metabolic labeling experiments showed that secretion of ＇＇S-apoA-I from Lipc-nuW cells was significantly higher than that from C57BL/6 cells.Expression of hHL in Lipc-nuW hepatocytes,through adenovirus-mediated gene transfer,resulted in decreased synthesis and secretion of ＇S-apoA-I,but not S-apoE,as compared with cells transfected with control adenovirus.Expression of HSPG-binding deficient hHLmt in Lipc-nuW cells also exerted an inhibitory effect on apoA-I production,even though hHLmt displayed impaired exit from the endoplasmic reticulum as compared with hHL.Subcellular fractionation revealed that expression of hHL or hHLmt led to increased microsome-association of apoA-I relative to non-transfected control.Expression of hHL negatively impacts hepatic production of apoA-I.

关键词： high density lipoprotein heparan sulphate proteoglycans ABCA1 endoplasmic reticulum adenovirus

Non-alcoholic fatty liver disease: a narrative review of genetics

在线全文

学校读者我要写书评

暂无评论

The Journal of Biomedical Research 2018年第6期32卷 389-400页

作者： Christopher J.Danford zemin yao Z.Gordon Jiang Division of Gastroenterology and Hepatology Beth Israel Deaconess Medical Center Department of Biochemistry Microbiology and Immunology Ottawa Institute of Systems Biology University of Ottawa

Non-alcoholic fatty liver disease(NAFLD) is now the most common cause of chronic liver diseases worldwide. It encompasses a spectrum of disorders ranging from isolated hepatic steatosis to nonalcoholic steatohepatitis(NASH),fibrosis, cirrhosis, and hepatocellular carcinoma. One of the key challenges in NAFLD is identifying which patients will progress. Epidemiological and genetic studies indicate a strong pattern of heritability that may explain some of the variability in NAFLD phenotype and risk of progression. To date, at least three common genetic variants in the PNPLA3, TM6 SF2, and GCKR genes have been robustly linked to NAFLD in the population. The function of these genes revealed novel pathways implicated in both the development and progression of NAFLD. In addition,candidate genes previously implicated in NAFLD pathogenesis have also been identified as determinants or modulators of NAFLD phenotype including genes involved in hepatocellular lipid handling, insulin resistance,inflammation, and fibrogenesis. This article will review the current understanding of the genetics underpinning the development of hepatic steatosis and the progression of NASH. These newly acquired insights may transform our strategy to risk-stratify patients with NAFLD and to identify new potential therapeutic targets.

关键词： NAFLD NASH genetics PNPLA3 TM6SF2 GCKR MBOAT7

Is vitamin D receptor a druggable target for non-alcoholic steatohepatitis?

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2020年第38期26卷 5812-5821页

作者： Ying Cao Xiang-Bing Shu zemin yao Guang Ji Li Zhang Institute of Digestive Diseases Longhua HospitalChina-Canada Center of Research for Digestive DiseasesShanghai University of Traditional Chinese MedicineShanghai 200032China Department of Geratology Baoshan Branch of Shuguang HospitalShanghai University of Traditional Chinese MedicineShanghai 201999China Department of Biochemistry Microbiology and ImmunologyUniversity of OttawaOttawa K1H8M5OntarioCanada

Nonalcoholic steatohepatitis(NASH)is a progressed stage of non-alcoholic fatty liver disease,and available therapeutic strategies for NASH are limited.Vitamin D receptor(VDR)is proposed as a druggable target for NASH due to the discovery of vitamin D deficiency in NASH patients.To date,vitamin D supplementation has not consistently conferred expected therapeutic benefits,raising the question of whether VDR can serve as a proper drug target for NASH.It is known that VDR can interact with other ligands such as bile acids in addition to vitamin D,and its expression can be induced by fatty acids,and insulin.It has also been shown that while activation of VDR in hepatic macrophages and hepatic stellate cells resulted in attenuation of hepatic inflammation and fibrosis,activation of VDR in hepatocytes could accelerate lipid accumulation.Thus,the multiplicity of VDR ligands,together with the cell type-specificity of VDR activation,must be taken into consideration in assessing the validity of VDR being a potential druggable target for NASH treatment.To this end,we have evaluated the relationship between VDR activation and various contributing factors,such as gut microbiota,bile acid,fatty acids,and insulin,in addition to vitamin D,with an expectation that a potential drug might be identified that can elicit VDR activation in a tissue-and/or cell type-specific manner and therefore achieving therapeutic benefits in NASH.

关键词： Vitamin D receptor Non-alcoholic steatohepatitis Vitamin D Bile acids Inflammation Lipid metabolism

Lipoprotein metabolism in nonalcoholic fatty liver disease

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The Journal of Biomedical Research 2013年第1期27卷 1-13页

作者： Zhenghui Gordon Jiang Simon C. Robson zemin yao Department of Medicine Beth Israel Deaconess Medical Center/Harvard Medical SchoolBostonMAUSA Division of Gastroenterology CLS612Beth Israel Deaconess Medical Center/Harvard Medical SchoolBostonMAUSA Department of Biochemistry Microbiology and ImmunologyOttawa Institute of Systems BiologyUniversity of OttawaOttawaOntario KIH 8M5Canada

Nonalcoholic fatty liver disease （NAFLD）, an pathologies characterized by fatty accumulation in escalating health problem worldwide, covers a spectrum of hepatocytes in early stages, with potential progression to liver inflammation, fibrosis, and failure. A close, yet poorly understood link exists between NAFLD and dyslipidemia, a constellation of abnormalities in plasma lipoproteins including triglyceride-rich very low density lipoproteins. Apolipoproteins are a group of primarily liver-derived proteins found in serum lipoproteins; they not only play an extracellular role in lipid transport between vital organs through circulation, but also play an important intracellu- lar role in hepatic lipoprotein assembly and secretion. The liver functions as the central hub for lipoprotein metab- olism, as it dictates lipoprotein production and to a significant extent modulates lipoprotein clearance. Lipoprotein metabolism is an integral component of hepatocellular lipid homeostasis and is implicated in the pathogenesis, potential diagnosis, and treatment of NAFLD.

关键词： nonalcoholic fatty liver disease （NAFLD） hepatic steatosis nonalcoholic steatohepatitis apolipo-protein lipoprotein metabolism very low density lipoprotein

Unraveling complexity of interconnected regulatory circuits in lipid metabolism

在线全文

学校读者我要写书评

暂无评论

The Journal of Biomedical Research 2014年第3期28卷 153-154页

作者： zemin yao Department of Biochemistry Microbiology and ImmunologyOttawa Institute of Systems BiologyUniversity of Ottawa

In this issue of Journal of Biomedical Research,3review articles are published that cover a broad range of topics addressing current understanding on regulation of nutrient metabolism through protein phosphatases,homeostatic regulation of cellular lipid droplets by small GTPases,and mechanisms by which hepatic assembly and secretion of triglyceride-rich lipoproteins are regulated.

关键词： Unraveling complexity of interconnected regulatory circuits in lipid metabolism

Ultrafast dynamics of photoexcited carriers and coherent phonons in ultrathin Bi;Te;thermoelectric films

在线全文

学校读者我要写书评

暂无评论

Science China(Physics,Mechanics & Astronomy) 2022年第1期65卷 71-77页

作者： zemin Xu Jiandong yao Guowei Yang Tianshu Lai State Key Laboratory of Optoelectronic Materials and Technologies School of PhysicsSun Yat-sen UniversityGuangzhou 510275China State Key Laboratory of Optoelectronic Materials and Technologies Nanotechnology Research CenterSchool of Materials Science&EngineeringSun Yat-sen UniversityGuangzhou 510275China

The ultrafast dynamics of photoexcited carriers and coherent phonons in ultrathin Bi;Te;thermoelectric films are studied through transient differential transmission spectroscopy.An ultralow frequency coherent optical phonon at 0.16 THz emerges,especially in ultrathin films,and it is ascribed to interlayer breathing modes.It can divide the ultrathin films into two groups which have outof-phase vibration along the normal of a film plane,causing a destructive interference between in-plane propagating thermal waves in the two groups of quintuple layers,and thus possibly reducing the thermal conductivity of the ultrathin films.The excitation power dependence of ultrafast dynamics reveals carrier-carrier scattering dominating thermalization,which provides a microscopic understanding of the reported high electrical conductivity and anomalously high power factor of ultrathin Bi;Te;films at room temperature.

关键词： electron-electron interactions coherent phonons carrier dynamics thermoelectric effects