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Distinctive quality control method for solid-state fermented Isaria cicadae from strain Ic-17-7 and application in a rat model of type 2 diabetes

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Chinese Journal of Natural Medicines 2021年第12期19卷 921-929页

作者： SUN Chang-Sheng WANG Yu-Qin ZHANG Zhong-Liang LIOU Yuligh zhou hong-hao Department of Clinical Pharmacology Xiangya HospitalCentral South UniversityChangsha 410008China Institute of Clinical Pharmacology Central South UniversityHunan Key Laboratory of PharmacogeneticsChangsha 410078China Engineering Research Center of Applied Technology of Pharmacogenomics Ministry of EducationChangsha 410078China National Clinical Research Center for Geriatric Disorders Changsha 410008China China Zhejiang Bioasia Life Science Institute Pinghu 314200China

This work was aimed to establish a quality control method for evaluating the effects on glucose and lipids of the fruiting body of Isaria cicadae Miquel from strain Ic-17-7(Ic-17-7fb) using a rat model of type 2 diabetes(T2DM). Random amplified polymorphic DNA, sequence-characterized amplified region, and high-performance liquid chromatography(HPLC) were used for the quality control of Ic-17-7fb. The pharmacological effects on streptozocin(STZ)-induced high fat diet(HFD)-fed Albino Wistar rats were evaluated. The rats underwent the following treatments: control, metformin, Ic-17-7fb(0.166 and 0.5 g·kg;) or without treatment. The fasting blood glucose(FBG), blood glucose, total cholesterol(TC), triglyceride(TG), high-density lipoprotein(HDL-c), and low-density lipoprotein(LDL-c) were measured. Ic-17-7fb amplified a single specific band by S11-2-F3 and S11-2-R3 primers. An HPLC-based quality and quantity method was established for industrial application. The contents of adenosine and N;-(2-hydroxyethyl) adenosine(HEA) of the cultivated Ic-17-7fb were analyzed. All of the validation lots of cultured Ic-17-7fb passed the quantity control of the training set(0.90 mg·g;of adenosine and 0.89 mg·g;of HEA). After two weeks of administration, the average FBG was 4.89 ± 0.42(control), 26.10 ± 5.77(model), 23.63 ± 6.15(metformin), 17.96 ± 9.36(Ic-17-7fb for 0.166 g·kg;), and 19.69 ±8.71 mmol·L;(Ic-17-7fb for 0.5 g·kg;). The FBG of Ic-17-7fb(0.166 g·kg;) treatment significantly reduced by 31.19%, compared with the model after two weeks of administration(P < 0.01). Metformin, Ic-17-7fb(0.166 g·kg;), and Ic-17-7fb(0.5 g·kg;) reduced TC, TG, HDL-c, and LDL-c compared with the T2DM model treatment at the 6 th week of treatment(P < 0.05). This study established the first quality standard for Ic-17-7fb, which can be effectively applied in the treatment of T2DM. The reliable quality control method and pharmacological effect will broaden its application space.

关键词： Isaria cicadae Quality control Adenosine HEA Type 2 diabetes model Blood glucose and lipid

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Panax notoginseng saponins prevent colitis-associated colorectal cancer development: the role of gut microbiota

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Chinese Journal of Natural Medicines 2020年第7期18卷 500-507页

作者： CHEN Ling CHEN Man-Yun Shao Li ZHANG Wei RAO Tai zhou hong-hao HUANG Wei-Hua Department of General Surgery Xiangya HospitalCentral South UniversityChangsha 410008China Department of Clinical Pharmacology Xiangya HospitalCentral South UniversityChangsha 410008China Institute of Clinical Pharmacology Hunan Key Laboratory of PharmacogeneticsCentral South UniversityChangsha 410078China National Clinical Research Center for Geriatric Disorders Xiangya HospitalCentral South UniversityChangsha 410008China Department of Pharmacognosy School of PharmacyHunan University of Chinese MedicineChangsha 410128China

Gut microbiota dysbiosis is a risk factor for colorectal cancer(CRC) in inflammatory bowel disease(IBD).In this study, the effects of Panax notoginseng saponins(PNS) on colitis-associated CRC progression were evaluated on an azoxymethane(AOM)/dextran sulfate sodium(DSS) mouse *** vivo, PNS significantly relieved AOM/DSS-induced colon tumorigenesis and development by reducing the disease activity index(DAI) scores and colon tumor *** 16S rRNA data of fecal samples showed that the microbiome community was obviously destructed, while PNS could recover the richness and diversity of gut ***, PNS could increase the abundance of Akkermansia *** was significantly decreased in model group and negatively correlated with the progression of ***, ginsenoside compound K(GC-K) was evaluated on the effects of human CRC cells,which was the main bio-transformed metabolite of PNS by gut *** data showed that PNS played important role in the prevention of the progression of CRC, due to their regulation on the microbiome balance and microbial bio-converted product with antiCRC activity.

关键词： Panax notoginseng saponins Gut microbiota Colorectal cancer Ginsenosides 16S rRNA gene sequencing

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Quantification of Panax notoginseng saponins metabolites in rat plasma with in vivo gut microbiota-mediated biotransformation by HPLC-MS/MS

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Chinese Journal of Natural Medicines 2019年第3期17卷 231-240页

作者： GUO Yin-Ping CHEN Man-Yun Shao Li ZHANG Wei RAO Tai zhou hong-hao HUANG Wei-Hua Department of Clinical Pharmacology Xiangya Hospital Central South University Institute of Clinical Pharmacology Hunan Key Laboratory of Pharmacogenetics Central South University Department of Pharmacognosy School of Pharmacy Hunan University of Chinese Medicine

Panax notoginseng saponins(PNS) are the major components of Panax notoginseng, with multiple pharmacological activities but poor oral bioavailability. PNS could be metabolized by gut microbiota in vitro, while the exact role of gut microbiota of PNS metabolism in vivo remains poorly understood. In this study, pseudo germ-free rat models were constructed by using broad-spectrum antibiotics to validate the gut microbiota-mediated transformation of PNS in vivo. Moreover, a high performance liquid chromatography-electrospray ionization tandem mass spectrometry(HPLC-ESI-MS/MS) was developed for quantitative analysis of four metabolites of PNS, including ginsenoside F1(GF1), ginsenoside Rh2(GRh2), ginsenoside compound K(GCK) and protopanaxatriol(PPT). The results showed that the four metabolites could be detected in the control rat plasma, while they could not be determined in pseudo germ-free rat plasma. The results implied that PNS could not be biotransformed effectively when gut microbiota was disrupted. In conclusion, gut microbiota plays an important role in biotransformation of PNS into metabolites in vivo.

关键词： Panax notoginseng Gut microbiota HPLC-MS/MS Saponins Ginsenoside compound K Protopanaxatriol

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Numerical Modeling of Response and Damage of Masonry Walls to Blast Loading

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Transactions of Tianjin University 2006年第B09期12卷 132-137页

作者： DEEKS Andrew School of Civil and Resource Engineering The University of Western AustraliaAustralia

To model the damage process of masonry walls under blast loading, a dynamic continuum damage material model is constructed for brick and mortar separately. The degradation of both the stiffness and strength are governed by a damage variable. By using the proposed material model, damage and fragmentation of a typical masonry wall under blast loading at different scaled distances is calculated. The hazard level of the masonry wall to blast loading is evaluated by analyzing the numerical results.

关键词： masonry blast loading fragmentation hazard level damage numerical modeling

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Translational regulation of DNA repair systems by eIF3a in cancer chemotherapeutic response

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中国药理学与毒理学杂志 2016年第10期30卷 1050-1051页

作者： CHEN Juan CUI Jia-jia ZHANG Jian-ting zhou hong-hao LIU Zhao-qian YIN Ji-ye Department of Clinical Pharmacology Xiangya HospitalCentral South University Institute of Clinical Pharmacology Central South University Hunan Key Laboratory of Pharmacogenetics Department of Pharmacology and Toxicology Indiana University School of Medicine IU Simon Cancer Center Indiana University School of Medicine

OBJECTIVE To investigate the role of e IF3a in the regulation of DNA repair pathways in cancer chemotherapeutic *** Immunohistochemistry was used to determine the expression of e IF3a in lung and breast cancer tissues followed by association analysis of e IF3a expression with patient′s response to *** overexpression and RNA interference knockdown of e IF3a were carried out in NIH3T3and H1299 cell lines,respectively,to determine the effect of altered e IF3a expression on cellular response to chemotherapeutic drugs by using MTT *** DNA repair capacity of these cells was evaluated by using host-cell reactivation,NHEJ and HR ***-time reverse transcriptase PCR and Western Blot analyses were carried out to determine the effect of e IF3a on the DNA repair genes by using cells with altered e IF3a *** e IF3a expression associates with response of lung and breast cancer patients to platinum and anthracycline.e IF3a knockdown or overexpression,respectively,increased and decreased the cellular resistance to cisplatin and anthracycline anticancer drugs,DNA repair activity,and expression of NER and NHEJ DNA repair *** e IF3a plays an important role in regulating the expression of NER and NHEJ DNA repair proteins which,in turn,contributes to cellular response to DNA-damaging anticancer drugs and patients′response to platinum and anthracycline chemotherapy.

关键词： eIF3a DNA repair translation regulation platinum anthracycline cancer chemotherapy

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LINC01094 drives the progression of diffuse gliomas and affects the sensitivity of temozolomide treatment

LINC01094 drives the progression of diffuse gliomas and affe...

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2019年中国肿瘤标志物学术大会暨第十三届肿瘤标志物青年科学家论坛

作者： Zou,Hecun zhou,hong-hao Institute of Life Sciences Chongqing Medical University Xiangya Hospital Central South University

Objective Diffuse gliomas are the most common type of primary brain tumors in central nervous system(CNS),according to the 2016 WHO classification of CNS tumors,diffuse gliomas are generally categorized into astrocytoma,oligodendroglioma,oligoastrocytoma,and glioblastoma [1].The standard care for newly diagnosed diffuse gliomas including surgical resection,radiation therapy,

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雌激素受体基因多态性与乳腺癌易感性研究进展

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生理科学进展 2008年第3期39卷 239-242页

作者：周琳周宏灏中南大学临床药理研究所长沙410078

雌激素受体(ER)是雌激素发挥作用的关键,雌激素受体基因存在遗传多态性,目前已对雌激素受体基因多态性与乳腺癌易感性进行了多项研究。本文就雌激素受体基因的多态性及其与乳腺癌发生的关系进行了综述。

关键词：乳腺癌雌激素受体基因多态性

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Laser‑Induced Highly Stable Conductive Hydrogels for Robust Bioelectronics

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Nano-Micro Letters 2025年第3期17卷 117-120页

作者： Yibo Li hao zhou Huayong Yang Kaichen Xu State Key Laboratory of Fluid Power and Mechatronic Systems School of Mechanical EngineeringZhejiang UniversityHangzhou 310058People’s Republic of China

Despite the promising progress in conductive hydrogels made with pure conducting polymer,great challenges remain in the interface adhesion and robustness in longterm *** address these challenges,*** Hwan Ko and Taek-Soo Kim’s team introduced a laserinduced phase separation and adhesion method for fabricating conductive hydrogels consisting of pure poly(3,4-ethylenedioxythiophene):polystyrene sulfonate on polymer *** laser-induced phase separation and adhesion treated conducting polymers can be selectively transformed into conductive hydrogels that exhibit wet conductivities of 101.4 S cm^(−1)with a spatial resolution down to 5μ***,they maintain impedance and charge-storage capacity even after 1 h of *** micropatterned electrode arrays demonstrate their potential in long-term in vivo signal recordings,highlighting their promising role in the field of bioelectronics.

关键词： Laser processing Conductive hydrogels Stable interface Bio-interfacing electrodes Bioelectronic application

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代谢组学及其在药理学中的进展

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中国药理学通报 2006年第11期22卷 1304-1309页

作者：何君周宏灏中南大学临床药理研究所湖南长沙410078

随着化学检测技术和数据处理技术的发展,代谢组学成为了基因功能研究的重要手段,该文对代谢组学的基本知识和基本技术进行了介绍,对其在药理学研究中的应用作了综述和展望。

关键词：代谢组学药理学

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黄酮类化合物对细胞色素P450 CYP1,2E1,3A4和19的影响

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药学学报 2007年第1期42卷 8-12页

作者：郑姣周宏灏中南大学临床药理研究所湖南长沙410078

黄酮类化合物广泛存在于蔬菜、坚果、水果和饮料及中草药中,可诱导或抑制多种细胞色素P450的活性。本篇综述主要集中回顾黄酮类化合物对于细胞色素P450CYP1,2E1,3A4和19的影响。归纳总结了该类物质抑制和诱导细胞色素P450的多种机制,如... 详细信息

黄酮类化合物广泛存在于蔬菜、坚果、水果和饮料及中草药中,可诱导或抑制多种细胞色素P450的活性。本篇综述主要集中回顾黄酮类化合物对于细胞色素P450CYP1,2E1,3A4和19的影响。归纳总结了该类物质抑制和诱导细胞色素P450的多种机制,如刺激特定的受体、稳定相关mRNA等。并总结了该类物质对细胞色素P450的影响体内和体外水平的研究结果并非总是一致的原因,如体内外的浓度的差异、基因和其他环境因素的影响。由于黄酮类化合物可通过影响细胞色素P450的活性影响药物代谢从而导致药物不良反应和药物相互作用,因此在将该类物质与其他药物合用时应高度重视。

关键词：黄酮类化合物细胞色素P450 诱导抑制

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